Alterations of miR-132 are novel diagnostic biomarkers in peripheral blood of schizophrenia patients
Abstract
Alterations in microRNAs (miRNAs) have been considered to have diagnostic implications in most diseases, but few studies have reported dysregulated miRNAs in schizophrenia (SCZ). In order to observe an association between miRNAs and SCZ, this study was designed to investigate expression profiling of miRNAs in peripheral blood mononuclear cells (PBMCs). miRNA microarray technology was employed to compare the expression of miRNAs in PBMCs from SCZ patients (n=105) and normal controls (n=130), and real-time quantitative polymerase chain reaction (QPCR) was used to analyze the results. Several important miRNA levels were examined before and after antipsychotic treatment in first-onset SCZ patients. In addition, an SCZ-like rat model was established using dizocilpine (MK-801), and miR-132 expression in PBMCs and whole-brain tissue from SCZ-like rats was studied using QPCR. In humans, dysregulated miRNAs were observed before treatment and QPCR verified that miR-132, miR-134, miR-1271, miR-664(⁎), miR-200c and miR-432 levels were significantly decreased (P<0.01 for all) in PBMCs of SCZ patients compared with healthy controls. After antipsychotic treatment there was a marked increase in miR-132 (P<0.01), miR-664(⁎) (P<0.05) and miR-1271 (P<0.05) levels in SCZ patients compared with the levels before treatment. In the animal assays, miR-132 levels declined in PBMCs and whole-brain tissues (both P<0.05) of the SCZ-like rats compared to controls. For the first time, our results suggest that miR-132 is a potential and superior biomarker in peripheral blood that will allow discrimination of SCZ patients from healthy controls.
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Antipsychotic treatment increases miR-132, miR-664, and miR-1271 compared with pre-treatment levels.
“antipsychotic treatment increases miR-132, miR-664, and miR-1271 compared with pre-treatment levels”
From The clinical use of epigenetics in psychiatry: a narrative review of epigenetic mechanisms, key candidate genes, and precision psychiatry (2025) · cites this paper as [37]
In schizophrenia, miR-132, miR-134, miR-1271, miR-664, miR-200c, and miR-432 are significantly decreased.
“In schizophrenia, miR-132, miR-134, miR-1271, miR-664, miR-200c, and miR-432 are significantly decreased”
From The clinical use of epigenetics in psychiatry: a narrative review of epigenetic mechanisms, key candidate genes, and precision psychiatry (2025) · cites this paper as [37]
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- Authors
- Yu, H.-C., Wu, J., Zhang, H.-X., Zhang, G.-L., Sui, J., Tong, W.-W., Zhang, X.-Y., Nie, L.-L., Duan, J.-H., Zhang, L.-R., & Lv, L.-X.
- Affiliations
- Henan Medical University (CN)
- Venue
- Progress in Neuro-Psychopharmacology and Biological Psychiatry
- Publisher
- Elsevier BV
- Published
- 2015-12
- Volume
- 63
- Pages
- 23-29
- ISSN
- 0278-5846 (ISSN-L)
- OpenAlex
- W224004219
- Cited by
- 71
- References
- 32
- Citations by year
- 2026: 4 · 2025: 8 · 2024: 9 · 2023: 10 · 2022: 6 · 2021: 6 · 2020: 7 · 2019: 6 · 2018: 4 · 2017: 3 · 2016: 7 · 2015: 1
- FWCI
- 4.08
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Progress in Neuro-Psychopharmacology and Biological Psychiatry (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Psychiatry and Mental health › Schizophrenia research and treatment
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › MicroRNA in disease regulation
- Life Sciences › Neuroscience › Biological Psychiatry › Tryptophan and brain disorders
- Keywords
- schizophrenia, miR-132, miRNA expression profiling, peripheral blood mononuclear cells, diagnostic biomarkers, antipsychotic treatment response, miR-134, miR-200c, miR-432, first-episode schizophrenia
- MeSH terms
- Adolescent, Adult, Animals, Exploratory Behavior, Female, Gene Expression Regulation, Humans, Interpersonal Relations, Leukocytes, Mononuclear, Male, Retrospective Studies, ROC Curve, Schizophrenia, Antipsychotic Agents, Biomarkers, Chi-Square Distribution, Dizocilpine Maleate, Rats, Sprague-Dawley, Excitatory Amino Acid Antagonists, Oligonucleotide Array Sequence Analysis, Gene Expression Profiling, MicroRNAs, Rats, Young Adult
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