In vivo prediction of the nutrient status of individual microalgal cells using Raman microspectroscopy
Abstract
An in vivo method for predicting the nutrient status of individual algal cells using Raman microspectroscopy is described. Raman spectra of cells using 780 nm laser excitation show enhanced bands mainly attributable to chlorophyll a and beta-carotene. The relative intensities of chlorophyll a and beta-carotene bands changed under nitrogen limitation, with chlorophyll a bands becoming less intense and beta-carotene bands more prominent. Although spectra from N-replete and N-starved cell populations varied, each distribution was distinct enough such that multivariate classification methods, such as partial least squares discriminant analysis, could accurately predict the nutrient status of the cells from the Raman spectral data.
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Temporally segmented modelling can be used to focus efforts on highly controlled growth and production phases in bioprocesses to develop models, terminating when a robust soft sensor model has been correlated to a CPP already in use (e.g. pH).
“Temporally segmented modelling can be used to focus efforts on highly controlled growth and production phases in bioprocesses to develop models [115], terminating when a robust soft sensor model has been correlated to a CPP already in use (e.g. pH).”
From Microalgal process-monitoring based on high-selectivity spectroscopy tools: status and future perspectives (2018) · cites this paper as [115]
The pattern of spectra and bands present in M. contortum and Chlamydomonas sp. conform to the spectra of green microalgae.
“The pattern of the spectra and the bands present in the two species conform to the spectra of the green microalgae [18,23,53,55].”
From Response to nutrient variation on lipid productivity in green microalgae captured using second derivative FTIR and Raman spectroscopy (2022) · cites this paper as [18]
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- Authors
- Heraud, P., Beardall, J., McNaughton, D., & Wood, B. R.
- Affiliations
- Monash University (AU)
- Corresponding author
- Heraud, Philip
- Venue
- FEMS Microbiology Letters
- Publisher
- Oxford University Press (OUP)
- Published
- 2007-10
- Volume
- 275
- Issue
- 1
- Pages
- 24-30
- ISSN
- 0378-1097 (ISSN-L)
- 1574-6968
- OpenAlex
- W1987151484
- Cited by
- 102
- References
- 34
- Citations by year
- 2026: 2 · 2025: 8 · 2024: 3 · 2023: 2 · 2022: 7 · 2021: 9 · 2020: 3 · 2019: 9 · 2018: 4 · 2017: 7 · 2016: 7 · 2015: 6 · 2014: 8 · 2013: 6 · 2012: 6
- FWCI
- 1.97
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- FEMS Microbiology Letters (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Spectroscopy Techniques in Biomedical and Chemical Research
- Physical Sciences › Chemistry › Analytical Chemistry › Spectroscopy and Chemometric Analyses
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Keywords
- microalgae, nutrient status, micro-Raman spectroscopy, chlorophyll a, beta-carotene, nitrogen limitation, nitrogen starvation, PLS-DA
- MeSH terms
- Chlorophyll A, Chlorophyta, Chlorophyll, Lasers, Nitrogen, Spectrum Analysis, Raman, beta Carotene
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