The DOI matches its Crossref record; 7 of the 7 references it cites were found in a registry or an index.
Derivation and Expansion of PAX7-Positive Muscle Progenitors from Human and Mouse Embryonic Stem Cells
Open accessAbstract
Cell therapies treating pathological muscle atrophy or damage requires an adequate quantity of muscle progenitor cells (MPCs) not currently attainable from adult donors. Here, we generate cultures of approximately 90% skeletal myogenic cells by treating human embryonic stem cells (ESCs) with the GSK3 inhibitor CHIR99021 followed by FGF2 and N2 supplements. Gene expression analysis identified progressive expression of mesoderm, somite, dermomyotome, and myotome markers, following patterns of embryonic myogenesis. CHIR99021 enhanced transcript levels of the pan-mesoderm gene T and paraxial-mesoderm genes MSGN1 and TBX6; immunofluorescence confirmed that 91% ± 6% of cells expressed T immediately following treatment. By 7 weeks, 47% ± 3% of cells were MYH(+ve) myocytes/myotubes surrounded by a 43% ± 4% population of PAX7(+ve) MPCs, indicating 90% of cells had achieved myogenic identity without any cell sorting. Treatment of mouse ESCs with these factors resulted in similar enhancements of myogenesis. These studies establish a foundation for serum-free and chemically defined monolayer skeletal myogenesis of ESCs.
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Asymmetric self-renewal and commitment of satellite stem cells in muscle
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A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo
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Differentiation of pluripotent stem cells to muscle fiber to model Duchenne muscular dystrophy
Chal, J., Oginuma, M., Al Tanoury, Z., Gobert, B., Sumara, O., Hick, A., Bousson, F., Zidouni, Y., Mursch, C., Moncuquet, P., et al. (2015) · Nature Biotechnology · doi:10.1038/nbt.3297 · Cited by 427
Engineering human pluripotent stem cells into a functional skeletal muscle tissue
Rao, L., Qian, Y., Khodabukus, A., Ribar, T., & Bursac, N. (2018) · Nature Communications · doi:10.1038/s41467-017-02636-4 · Cited by 334
Chal, J., Al Tanoury, Z., Hestin, M., Gobert, B., Aivio, S., Hick, A., Cherrier, T., Nesmith, A. P., Parker, K. K., & Pourquié, O. (2016) · Nature Protocols · doi:10.1038/nprot.2016.110 · Cited by 299
Metadata
- Authors
- Shelton, M., Metz, J., Liu, J., Carpenedo, R. L., Demers, S.-P., Stanford, W. L., & Skerjanc, I. S.
- Venue
- Stem Cell Reports
- Publisher
- Elsevier BV
- Published
- 2014-09
- Volume
- 3
- Issue
- 3
- Pages
- 516-529
- ISSN
- 2213-6711 (ISSN-L)
- OpenAlex
- W2015703333
- Cited by
- 182
- References
- 7
- FWCI
- 6.33
- Type
- journal article
- Language
- en
- Open access
- gold
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- Shelton, M., Metz, J., Liu, J., Carpenedo, R. L., Demers, S.-P., Stanford, W. L., & Skerjanc, I. S. (2014). Derivation and Expansion of PAX7-Positive Muscle Progenitors from Human and Mouse Embryonic Stem Cells. Stem Cell Reports. https://doi.org/10.1016/j.stemcr.2014.07.001
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
