Comparative expression of the mouse Sox1, Sox2 and Sox3 genes from pre-gastrulation to early somite stages
Open accessAbstract
Whole mount in situ hybridisation was used to study the embryonic expression of the mouse HMG box-containing genes Sox1, Sox2 and Sox3 between 6.5 and 9.0 days post coitum (dpc). Sox2 and Sox3 are expressed in the epiblast and extraembryonic ectoderm of the egg cylinder, becoming restricted to the prospective neural plate and chorion at the onset of gastrulation. Sox3 is upregulated in the posterior ectoderm during late streak and neural plate stages and is concomitantly downregulated in the chorion. Sox1 transcripts are first detected in the neural fold ectoderm at the headfold stage. During early somitogenesis, all three genes are expressed in the neuroectoderm, and Sox2 and Sox3 are also expressed in the primitive streak ectoderm, gut endoderm and prospective sensory placodes.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Sox2 is expressed in both ectoderm and endoderm libraries.
“Similarly, Sox2 is expressed in both ectoderm and endoderm libraries corresponding to published expression patterns [38].”
From A systematic screen for genes expressed in definitive endoderm by Serial Analysis of Gene Expression (SAGE) (2007) · cites this paper as [38]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
In situ hybridization; a practical approach
(1993) · International Journal of Biochemistry · doi:10.1016/0020-711x(93)90093-t · Cited by 1,240 · see citing works
Staging of gastrulating mouse embryos by morphological landmarks in the dissecting microscope
Downs, K. M., & Davies, T. J. (1993) · Development · doi:10.1242/dev.118.4.1255 · Cited by 582 · see citing works
A comparison of the properties of Sox-3 with Sry and two related genes, Sox-1 and Sox-2
Collignon, J., Sockanathan, S., Hacker, A., Cohen‐Tannoudji, M., Norris, D. P., Rastan, S., Stevanović, M. J., Goodfellow, P. N., & Lovell-Badge, R. H. (1996) · Development · doi:10.1242/dev.122.2.509 · Cited by 496 · see citing works
Pevny, L., & Lovell-Badge, R. H. (1997) · Current Opinion in Genetics & Development · doi:10.1016/s0959-437x(97)80147-5 · Cited by 489 · see citing works
A role for SOX1 in neural determination
Pevny, L., Sockanathan, S., Placzek, M., & Lovell-Badge, R. H. (1998) · Development · doi:10.1242/dev.125.10.1967 · Cited by 475 · see citing works
Uwanogho, D., Rex, M., Cartwright, E. J., Pearl, G., Healy, C., Scotting, P. J., & Sharpe, P. T. (1995) · Mechanisms of Development · doi:10.1016/0925-4773(94)00299-3 · Cited by 391 · see citing works
Sox1 directly regulates the gamma -crystallin genes and is essential for lens development in micePreprint
Nishiguchi, S., Wood, H. B., Kondoh, H., Lovell-Badge, R. H., & Episkopou, V. (1998) · Genes & Development · doi:10.1101/gad.12.6.776 · Cited by 290 · see citing works
Self-citationGAL4 enhancer traps expressed in the embryo, larval brain, imaginal discs, and ovary of drosophila
Manseau, L. J., Baradaran, A., Brower, D. L., Budhu, A., Elefant, F., Phan, H., Philp, A. V., Yang, M., Glover, D. M., Kaiser, K., et al. (1997) · Developmental Dynamics · doi:10.1002/(sici)1097-0177(199707)209:3 · Cited by 241 · see citing works
Clonal Analysis of Cell Fate During Gastrulation and Early Neurulation in the Mouse
Lawson, K. A., & Pedersen, R. A. (2007) · Novartis Foundation symposium · doi:10.1002/9780470514221.ch2 · Cited by 177 · see citing works
Preventing the loss of competence for neural induction: HGF/SF, L5 and Sox-2
Streit, A., Sockanathan, S., Pérez, L., Rex, M., Scotting, P. J., Sharpe, P. T., Lovell-Badge, R. H., & Stern, C. D. (1997) · Development · doi:10.1242/dev.124.6.1191 · Cited by 159 · see citing works
Establishment and Organization of Germ Layers in the Gastrulating Mouse Embryo
Tam, P., & Beddington, R. S. P. (2007) · Novartis Foundation symposium · doi:10.1002/9780470514221.ch3 · Cited by 150 · see citing works
Rex, M., Uwanogho, D., Orme, A. T., Scotting, P. J., & Sharpe, P. T. (1997) · Mechanisms of Development · doi:10.1016/s0925-4773(97)00086-5 · Cited by 50 · see citing works
Characterisation and mapping of the human SOX14 gene
Arsic, N., Rajic, T., Stanojčić, S., Goodfellow, P. N., & Stevanović, M. J. (1998) · Cytogenetic and Genome Research · doi:10.1159/000015149 · Cited by 26 · see citing works
Ciba Foundation Symposium 165 ‐ Postimplantation Development in the Mouse
(2007) · Novartis Foundation symposium · doi:10.1002/9780470514221 · Cited by 17 · see citing works
Flagged first, then by citations
2 of the 16 references couldn’t be matched to a known paper.
Cited by
Sorted by citations
Multipotent cell lineages in early mouse development depend on SOX2 functionPreprint
Avilion, A. A., Nicolis, S. K., Pevny, L., Pérez, L., Vivian, N., & Lovell-Badge, R. H. (2003) · Genes & Development · doi:10.1101/gad.224503 · Cited by 2,372 · see citing works
“1B) and later in the ExE portion of the chorion (Fig. 1C–E; Wood and Episkopou 1999).”
BackgroundInfluentialDirected differentiation of embryonic stem cells into motor neurons.
Wichterle, H., Lieberam, I., Porter, J. A., & Jessell, T. (2002) · Cell · doi:10.1016/s0092-8674(02)00835-8 · Cited by 1,792 · see citing works
Conversion of embryonic stem cells into neuroectodermal precursors in adherent monoculture
Ying, Q.-L., Stavridis, M., Griffiths, D., Li, M., & Smith, A. (2003) · Nature Biotechnology · doi:10.1038/nbt780 · Cited by 1,504 · see citing works
“Sox1 is the earliest known specific marker of neuroectoderm in the mouse embry”
BackgroundSOX2 Functions to Maintain Neural Progenitor Identity
Graham, V., Khudyakov, J. I., Ellis, P., & Pevny, L. (2003) · Neuron · doi:10.1016/s0896-6273(03)00497-5 · Cited by 1,344 · see citing works
“To address the role of SOX2 in CNS progenitors, we al., 1999; Pevny et al., 1998; Uchikawa et al., 2003; Uwaperformed a detailed study of SOX2 protein expression nogho et al., 1995; Wood and Episkopou, 1999 ).”
The Sox Family of Transcription Factors: Versatile Regulators of Stem and Progenitor Cell Fate
Sarkar, A., & Hochedlinger, K. (2013) · Cell stem cell · doi:10.1016/j.stem.2012.12.007 · Cited by 915 · see citing works
Directed differentiation of telencephalic precursors from embryonic stem cells
Watanabe, K., Kamiya, D., Nishiyama, A., Katayama, T., Nozaki, S., Kawasaki, H., Watanabe, Y., Mizuseki, K., & Sasai, Y. (2005) · Nature Neuroscience · doi:10.1038/nn1402 · Cited by 784 · see citing works
Ellis, P., Fagan, B. M., Magness, S. T., Hutton, S. R., Taranova, O., Hayashi, S., McMahon, A. P., Rao, M. S., & Pevny, L. (2004) · Developmental Neuroscience · doi:10.1159/000082134 · Cited by 733 · see citing works
Sox2 deficiency causes neurodegeneration and impaired neurogenesis in the adult mouse brain
Ferri, L., Cavallaro, M., Braida, D., Cristofano, A. D., Canta, A., Vezzani, A., Ottolenghi, S., Pandolfi, P. P., Sala, M., Debiasi, S., et al. (2004) · Development · doi:10.1242/dev.01204 · Cited by 666 · see citing works
“In many different species, Sox2 is a marker of the nervous system from the beginning of its development (Uwanogho et al., 1995; Wood and Episkopou, 1999; Kishi et al., 2000; Kamachi et al., 2000; Avilion et al., 2003); an important role in early neural development is further suggested by…”
BackgroundDepletion of definitive gut endoderm in <i>Sox17</i> -null mutant mice
Kanai-Azuma, M., Kanai, Y., Gad, J. M., Tajima, Y., Taya, C., Kurohmaru, M., Sanai, Y., Yonekawa, H., Yazaki, K., Tam, P. P. L., et al. (2002) · Development · doi:10.1242/dev.129.10.2367 · Cited by 704 · see citing works
Vertebrate Cranial Placodes I. Embryonic Induction
Baker, C. V. H., & Bronner-Fraser, M. E. (2001) · Developmental Biology · doi:10.1006/dbio.2001.0156 · Cited by 589 · see citing works
Sox proteins: regulators of cell fate specification and differentiation
Kamachi, Y., & Kondoh, H. (2013) · Development · doi:10.1242/dev.091793 · Cited by 568 · see citing works
“…(i.e. the nasal, lens, otic and taste bud placodes, as well as the hypophyseal placode), which are the precursors of sensory tissues, develop from the cephalic ectoderm and express Sox2 as a key regulator (Okubo et al., 2006; Schlosser et al., 2008; Uchikawa et al., 2011; Wood and Episkopou, 1999).”
BackgroundRegulated Fluctuations in Nanog Expression Mediate Cell Fate Decisions in Embryonic Stem Cells
Kalmár, T., Lim, C. L., Hayward, P. C., Descalzo, S. M., Nichols, J., García‐Ojalvo, J., & Arias, A. M. (2009) · PLoS Biology · doi:10.1371/journal.pbio.1000149 · Cited by 541 · see citing works
Distinct Lineage Specification Roles for NANOG, OCT4, and SOX2 in Human Embryonic Stem Cells
Wang, Z., Oron, E., Nelson, B., Razis, S., & Ivanova, N. B. (2012) · Cell stem cell · doi:10.1016/j.stem.2012.02.016 · Cited by 545 · see citing works
“During gastrulation, Sox2 becomes restricted to the anterior half of the embryo while Sox3 is upregulated throughout the eEC (Wood and Episkopou, 1999).”
BackgroundSox2 is required for sensory organ development in the mammalian inner ear
Kiernan, A. E., Pelling, A. L., Leung, K. K. H., Tang, A. S. P., Bell, D. M., TEASE, C., Lovell-Badge, R. H., Steel, K. P., & Cheah, K. S. E. (2005) · Nature · doi:10.1038/nature03487 · Cited by 538 · see citing works
Wnt Signaling Mediates Self-Organization and Axis Formation in Embryoid Bodies
ten Berge, D., Koole, W., Fuerer, C., Fish, M., Eroglu, E., & Nusse, R. (2008) · Cell Stem Cell · doi:10.1016/j.stem.2008.09.013 · Cited by 477 · see citing works
“In contrast, Dkk1 protein delayed the induction of Brachyury, FoxA2, and Flk1 (Figure 3B), just as it delayed Wnt-pathway activation (Figures 2B and 2C) and promoted expression of Otx2, Pax6, and Sox1 (Figure 3B).”
BackgroundInfluentialEfficient gene modulation in mouse epiblast using a Sox2Cre transgenic mouse strain
Hayashi, S., Lewis, P. M., Pevny, L., & McMahon, A. P. (2002) · Mechanisms of Development · doi:10.1016/s0925-4773(03)00099-6 · Cited by 492 · see citing works
van den Brink, S., Baillie‐Johnson, P., Balayo, T., Hadjantonakis, A., Nowotschin, S., Turner, D. A., & Arias, A. M. (2014) · Development · doi:10.1242/dev.113001 · Cited by 461 · see citing works
“Acknowledgements We thank K. Niakan for the Sox17::GFP cell line, E. Davies for sharing data and J. Brickman, J. Briscoe, S. Mun ̃oz-Descalzo, J. Nichols, A. Perea-Gomez, C. Schröeter, T. Rodriguez and C. Stern for discussions and constructive criticisms.”
InfluentialGenetic Regulation of Pituitary Gland Development in Human and Mouse
Kelberman, D., Rizzoti, K., Lovell-Badge, R. H., Robinson, I. C. A. F., & Dattani, M. (2009) · Endocrine Reviews · doi:10.1210/er.2009-0008 · Cited by 471 · see citing works
“Sox3, along with Sox2, is expressed throughout the CNS with particularly high levels of expression noted in the ventral diencephalon, including the infundibulum and presumptive hypothalamus (140, 141).”
BackgroundSakai, Y., Meno, C., Fujii, H., Nishino, J., Shiratori, H., Saijoh, Y., Rossant, J., & Hamada, H. (2001) · Genes & Development · doi:10.1101/gad.851501 · Cited by 458 · see citing works
“5) Expression domains of Sox2 (a marker for neural tissue; Kamachi et al. 1998; Wood and Episkopou 1999) were markedly expanded in the caudal region of CYP26−/− embryos (Fig.”
BackgroundTada, S., Era, T., Furusawa, C., Sakurai, H., Nishikawa, S., Kinoshita, M., Nakao, K., Chiba, T., & Nishikawa, S.-I. (2005) · Development · doi:10.1242/dev.02005 · Cited by 456 · see citing works
“The expression of Sox17 and the cytokeratins Krt2-8, cytokeratin 18 and Krt1-19 (Owens and Lane, 2003) was maintained during further culture of Gsc+ECD+ population in Development 132 (19) Research article D ev el op m en t 4369Identification and characterization of mesendoderm SCM, whereas the expression of Gsc and Mi…”
BackgroundInfluentialFrom stem cells to neurons and glia: a Soxist's view of neural development
Wegner, M., & Stolt, C. C. (2005) · Trends in Neurosciences · doi:10.1016/j.tins.2005.08.008 · Cited by 426 · see citing works
Neural induction: old problem, new findings, yet more questions
Stern, C. D. (2005) · Development · doi:10.1242/dev.01794 · Cited by 421 · see citing works
Uchikawa, M., Ishida, Y., Takemoto, T., Kamachi, Y., & Kondoh, H. (2003) · Developmental Cell · doi:10.1016/s1534-5807(03)00088-1 · Cited by 397 · see citing works
Induction and specification of cranial placodes
Schlosser, G. (2006) · Developmental Biology · doi:10.1016/j.ydbio.2006.03.009 · Cited by 389 · see citing works
“This group of genes includes Sox2 and Sox3, which together with Sox1 comprise the SoxB1 subfamily of HMG box containing transcription factors, as well as homeobox transcription factors Irx1 to Irx6 (also known as Xiro or Ziro genes in Xenopus and zebrafish, respectively) related to Drosophila Iroquois (Collignon et al…”
BackgroundSpatial and temporal patterns of ERK signaling during mouse embryogenesis
Corson, L. B., Yamanaka, Y., Lai, K.-M. V., & Rossant, J. (2003) · Development · doi:10.1242/dev.00669 · Cited by 390 · see citing works
Generation of anterior foregut endoderm from human embryonic and induced pluripotent stem cells
Green, M. D., Chen, A. F., Nostro, M. C., D’Souza, S. L., Schaniel, C., Lemischka, I. R., Gouon–Evans, V., Keller, G. M., & Snoeck, H. W. (2011) · Nature Biotechnology · doi:10.1038/nbt.1788 · Cited by 386 · see citing works
“FOXA2 is also expressed in the notochord (mesoderm), and FOXA2 and SOX2 are co-expressed by the hindbrain floorplate (neurectoderm”
Nostro, M. C., Sarangi, F., Ogawa, S., Holtzinger, A., Corneo, B., Li, X., Micallef, S. J., Park, I., Basford, C., Wheeler, M. B., et al. (2011) · Development · doi:10.1242/dev.055236 · Cited by 386 · see citing works
Tremblay, K. D., Dunn, N. R., & Robertson, E. J. (2001) · Development · doi:10.1242/dev.128.18.3609 · Cited by 379 · see citing works
SOX2 in development and cancer biology
Novak, D., Hüser, L., Elton, J. J., Umansky, V., Altevogt, P., & Utikal, J. S. (2019) · Seminars in Cancer Biology · doi:10.1016/j.semcancer.2019.08.007 · Cited by 369 · see citing works
Neurogenesis in zebrafish – from embryo to adult
Viales, R. R., Strähle, U., & Scholpp, S. (2013) · Neural Development · doi:10.1186/1749-8104-8-3 · Cited by 374 · see citing works
“The neural ectoderm is specified by members of the SoxB1 family [52-56].”
BackgroundKelberman, D. (2006) · Journal of Clinical Investigation · doi:10.1172/jci28658 · Cited by 370 · see citing works
“5 dpc it is expressed throughout the brain, neural tube, sensory placodes, branchial arches, and gut endoderm (6).”
BackgroundSOX9 induces and maintains neural stem cells
Scott, C. E., Wynn, S. L., Sesay, A. K., Cruz, C., Cheung, M. C. H., Gómez‐Gaviro, M. V., Booth, S., Gao, B., Cheah, K. S. E., Lovell-Badge, R. H., et al. (2010) · Nature Neuroscience · doi:10.1038/nn.2646 · Cited by 356 · see citing works
“Notably, SOX2 is expressed in the epiblast, as well as by embryonic stem cells (ESCs), and subsequently in cells of the neuroepithelium and multipotent NSC”
BackgroundZappone, M., Galli, R., Catena, R., Meani, N., Biasi, S. D., Mattei, E., Tiveron, C., Vescovi, A. L., Lovell-Badge, R. H., Ottolenghi, S., et al. (2000) · Development · doi:10.1242/dev.127.11.2367 · Cited by 349 · see citing works
“7 and data not shown) are expressed, as expected, in both spinal cordand cortex-derived neurospheres, several dorsal telencephalic genes (BF1, Xuan et al., 1995; Otx1, Simeone et al., 1993, Frantz et al., 1994; Tbr2, Bulfone et al., 1999) are expressed specifically in cortex-, but not in spinal…”
BackgroundIntegration of spatial and single-cell transcriptomic data elucidates mouse organogenesis
Lohoff, T., Ghazanfar, S., Missarova, A. M., Koulena, N., Pierson, N. G., Griffiths, J. A., Bardot, E., Eng, C.-H. L., Tyser, R. C. V., Argelaguet, R., et al. (2021) · Nature Biotechnology · doi:10.1038/s41587-021-01006-2 · Cited by 349 · see citing works
Osumi, N., Shinohara, H. M., Numayama‐Tsuruta, K., & Maekawa, M. (2008) · Stem Cells · doi:10.1634/stemcells.2007-0884 · Cited by 345 · see citing works
Sox2 roles in neural stem cells
Pevny, L., & Nicolis, S. K. (2009) · The International Journal of Biochemistry & Cell Biology · doi:10.1016/j.biocel.2009.08.018 · Cited by 337 · see citing works
Kurimoto, K., Yabuta, Y., Ohinata, Y., Shigeta, M., Yamanaka, K., & Saitou, M. (2008) · Genes & Development · doi:10.1101/gad.1649908 · Cited by 338 · see citing works
“D, panels iii,iv; Wood and Episkopou 1999) would also be an effect of mesoderm-inducing activities.”
BackgroundOkada, Y., Shimazaki, T., Sobue, G., & Okano, H. (2004) · Developmental Biology · doi:10.1016/j.ydbio.2004.07.038 · Cited by 333 · see citing works
“By contrast, RT-PCR analysis on day 6 showed that expression of sox1 (a marker of undifferentiated neural cells; Pevny et al., 1998; Wood and Episkopou, 1999) mRNA was higher in EBs treated with low-RA on day 6 (Fig.”
BackgroundMethodThe status of Wnt signalling regulates neural and epidermal fates in the chick embryo
Wilson, S., Rydström, A., Trimborn, T., Willert, K., Nusse, R., Jessell, T. M., & Edlund, H. (2001) · Nature · doi:10.1038/35077115 · Cited by 323 · see citing works
SOX genes and neural progenitor identity
Pevny, L., & Placzek, M. (2005) · Current Opinion in Neurobiology · doi:10.1016/j.conb.2005.01.016 · Cited by 318 · see citing works
SOX3 is required during the formation of the hypothalamo-pituitary axis
Rizzoti, K., Brunelli, S., Carmignac, D. F., Thomas, P. Q., Robinson, I. C. A. F., & Lovell-Badge, R. H. (2004) · Nature Genetics · doi:10.1038/ng1309 · Cited by 313 · see citing works
de Putte, T. V., Maruhashi, M., Francis, A., Nelles, L., Kondoh, H., Huylebroeck, D., & Higashi, Y. (2003) · The American Journal of Human Genetics · doi:10.1086/346092 · Cited by 309 · see citing works
“Finally, the aberrant regulation of E-cadherin gene expression in the neural ectoderm and the decreased expression of the neural transcription factor Sox2 may be relevant to neurological problems in patients with Hirschsprung disease–mental retardation syndrome, because these genes, as well as SIP1 (Yamada et al. 2001…”
BackgroundInfluentialIdentification of Sox-2 regulatory region which is under the control of Oct-3/4-Sox-2 complex
Tomioka, M. (2002) · Nucleic Acids Research · doi:10.1093/nar/gkf435 · Cited by 307 · see citing works
A discrete period of FGF-induced Erk1/2 signalling is required for vertebrate neural specification
Stavridis, M. P., Lunn, J. S., Collins, B., & Storey, K. G. (2007) · Development · doi:10.1242/dev.02858 · Cited by 296 · see citing works
“This is indicated by the onset of expression of genes characteristic of neuroepithelium, which include members of the SoxB family of transcription factors (Pevny et al., 1998; Rex et al., 1997; Wood and Episkopou, 1999).”
BackgroundWhat Is Trophoblast? A Combination of Criteria Define Human First-Trimester Trophoblast
Lee, C. Q. E., Gardner, L., Turco, M. Y., Zhao, N., Murray, M. J., Coleman, N., Rossant, J., Hemberger, M., & Moffett, A. (2016) · Stem Cell Reports · doi:10.1016/j.stemcr.2016.01.006 · Cited by 291 · see citing works
Interplay of SOX and POU Factors in Regulation of the Nestin Gene in Neural Primordial Cells
Tanaka, S., Kamachi, Y., Tanouchi, A., Hamada, H., Jing, N., & Kondoh, H. (2004) · Molecular and Cellular Biology · doi:10.1128/mcb.24.20.8834-8846.2004 · Cited by 277 · see citing works
“Anti-group B1 SOX antibodies (with demonstrated preference for SOX1 and SOX3 over SOX2 and indicated as anti-SOX1/[2]/3 in the figures) were derived from a rabbit similarly immunized with the SOX3 C-terminal 15 peptides.”
BackgroundMethodInfluentialAubert, J., Stavridis, M. P., Tweedie, S., O’Reilly, M., Vierlinger, K., Li, M., Ghazal, P., Pratt, T., Mason, J. O., Roy, D. J., et al. (2003) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1734197100 · Cited by 248 · see citing works
Lineage choice and differentiation in mouse embryos and embryonic stem cells
Loebel, D. A. F., Watson, C. M., Young, R. A. D., & Tam, P. (2003) · Developmental Biology · doi:10.1016/s0012-1606(03)00390-7 · Cited by 245 · see citing works
“The Sry-related HMG box-containing transcription factor Sox1 is expressed during early neurogenesis, becoming progressively downregulated as cells exit from mitosis (Pevny et al., 1998; Wood and Episkopou, 1999).”
BackgroundSOX2 functions in adult neural stem cells
Episkopou, V. (2005) · Trends in Neurosciences · doi:10.1016/j.tins.2005.03.003 · Cited by 242 · see citing works
Self-citationde Jong, J., Stoop, H., Gillis, A., van Gurp, R., van de Geijn, G., de Boer, M., Hersmus, R., Saunders, P. T. K., Anderson, R., Oosterhuis, J. W., et al. (2008) · The Journal of Pathology · doi:10.1002/path.2332 · Cited by 241 · see citing works
“Besides ES cells, SOX2 is abundantly expressed in differentiating and differentiated cells and is found in endodermal cells of the primitive foregut [58], the developing inner ear [59], and the central nervous system [60].”
BackgroundDistinct Wnt-driven primitive streak-like populations reflect in vivo lineage precursors
Tsakiridis, A., Huang, Y., Blin, G., Skylaki, S., Wymeersch, F. J., Osorno, R., Economou, C. N., Karagianni, E., Zhao, S., Lowell, S., et al. (2014) · Development · doi:10.1242/dev.101014 · Cited by 230 · see citing works
“To this end, an EpiSC line was established from 46C ESCs that carry a GFP reporter within the neurectoderm-specific Sox1 locus (Wood and Episkopou, 1999; Ying et al., 2003).”
MethodSox2 and Jagged1 Expression in Normal and Drug-Damaged Adult Mouse Inner Ear
Oesterle, E. C., Campbell, S., Taylor, R., Forge, A., & Hume, C. R. (2007) · Journal of the Association for Research in Otolaryngology · doi:10.1007/s10162-007-0106-7 · Cited by 234 · see citing works
“SCs in developing mouse inner ear express Sox2 and Jagged1, but their expression patterns in the adult remain to be characterized (e.g., Wood and Episkopou 1999; Woods et al. 2004; Kiernan et al. 2005a, b, 2006).”
BackgroundGure, A. O., Stockert, E., Scanlan, M. J., Keresztes, R. S., Jäger, D., Altorki, N. K., Old, L. J., & Chen, Y. (2000) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.97.8.4198 · Cited by 234 · see citing works
Ragge, N., Lorenz, B., Schneider, A. S., Bushby, K. M. D., SANCTIS, L. D., de Sanctis, U., Salt, A., Collin, J. R. O., Vivian, A. J., Free, S. L., et al. (2005) · American Journal of Medical Genetics Part A · doi:10.1002/ajmg.a.30642 · Cited by 230 · see citing works
“Case 5 had severe sensorineural hearing loss consistent with the expression of Sox2 in the developing otic vesicle during mouse [Wood and Episkopou, 1999] and zebrafish development (zfin.org).”
InfluentialNeural Differentiation of Embryonic Stem Cells In Vitro: A Road Map to Neurogenesis in the Embryo
Abranches, E., Silva, M. R., Pradier, L., Schulz, H., Hummel, O., Henrique, D., & Bekman, E. (2009) · PLoS ONE · doi:10.1371/journal.pone.0006286 · Cited by 225 · see citing works
“In the mouse embryo, the initial population of specified neuroepithelial progenitors (NPs) is known to express various pan-neural genes, like sox1 and sox2 [4,5].”
BackgroundMutations in SOX2 cause anophthalmia-esophageal-genital (AEG) syndrome
Williamson, K. A., Hever, A. M., Rainger, J., Rogers, R. C., Magee, A. C., Fiedler, Z., Keng, W. T., Sharkey, F. H., McGill, N. I., Hill, C. J., et al. (2006) · Human Molecular Genetics · doi:10.1093/hmg/ddl064 · Cited by 223 · see citing works
Mori, T., Buffo, A., & Götz, M. (2005) · Current topics in developmental biology/Current Topics in Developmental Biology · doi:10.1016/s0070-2153(05)69004-7 · Cited by 212 · see citing works
Absence of Nodal signaling promotes precocious neural differentiation in the mouse embryo
Camus, A., Perea-Gómez, A., Moreau, A., & Collignon, J. (2006) · Developmental Biology · doi:10.1016/j.ydbio.2006.03.047 · Cited by 209 · see citing works
“, 1995) ; Sox1 (Wood and Episkopou, 1999) ; Vax1 (Hallonet et al.”
Genetic and functional differences between multipotent neural and pluripotent embryonic stem cells
D’Amour, K. A., & Gage, F. H. (2003) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1834200100 · Cited by 210 · see citing works
Genetics of Combined Pituitary Hormone Deficiency: Roadmap into the Genome Era
Fang, Q., George, A. S., Brinkmeier, M. L., Mortensen, A. H., Gergics, P., Cheung, L., Daly, A. Z., Ajmal, A., Millan, M. I. P., Ozel, A. B., et al. (2016) · Endocrine Reviews · doi:10.1210/er.2016-1101 · Cited by 208 · see citing works
“cluding the developing brain, pituitary, and the otic and nasal placodes (219, 220).”
BackgroundSchlosser, G. (2010) · International review of cytology · doi:10.1016/s1937-6448(10)83004-7 · Cited by 195 · see citing works
Sox3 Is Required for Gonadal Function, but Not Sex Determination, in Males and Females
Weiss, J., Meeks, J. J., Hurley, L., Raverot, G., Frassetto, A., & Jameson, J. L. (2003) · Molecular and Cellular Biology · doi:10.1128/mcb.23.22.8084-8091.2003 · Cited by 195 · see citing works
“Sox3 is also expressed in the brain from the earliest stages of development (46), preceding gonadal expression, suggesting a role for Sox3 in brain formation and cognitive function.”
BackgroundHitoshi, S., Seaberg, R. M., Koscik, C., Alexson, T. O., Kusunoki, S., KANAZAWA, I., Tsuji, S., & van der Kooy, D. (2004) · Genes & Development · doi:10.1101/gad.1208404 · Cited by 190 · see citing works
Evolution of bilaterian central nervous systems: a single origin?
Holland, L. Z., Carvalho, J. E., Escrivá, H., Laudet, V., Schubert, M., Shimeld, S. M., & Yu, J. (2013) · EvoDevo · doi:10.1186/2041-9139-4-27 · Cited by 186 · see citing works
Shelton, M., Metz, J., Liu, J., Carpenedo, R. L., Demers, S.-P., Stanford, W. L., & Skerjanc, I. S. (2014) · Stem Cell Reports · doi:10.1016/j.stemcr.2014.07.001 · Cited by 183 · see citing works
SOX2 Plays a Critical Role in the Pituitary, Forebrain, and Eye during Human Embryonic Development
Kelberman, D., de Castro, S. C., Huang, S., Crolla, J. A., Palmer, R. W., Gregory, J. W., Taylor, D. S. I., Cavallo, L., Faienza, M. F., Fischetto, R., et al. (2008) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jc.2007-2337 · Cited by 181 · see citing works
“5 is expressed throughout the central nervous system, sensory placodes, branchial arches, and gut endoderm, including the developing esophagus (1, 6, 7).”
BackgroundSOX family transcription factors involved in diverse cellular events during development
She, Z., & Yang, W. (2015) · European Journal of Cell Biology · doi:10.1016/j.ejcb.2015.08.002 · Cited by 174 · see citing works
Having it both ways: Sox protein function between conservation and innovation
Guth, S. I. E., & Wegner, M. (2008) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-008-8138-7 · Cited by 176 · see citing works
Sox3 expression in undifferentiated spermatogonia is required for the progression of spermatogenesis
Raverot, G., Weiss, J., Park, S. Y., Hurley, L., & Jameson, J. L. (2005) · Developmental Biology · doi:10.1016/j.ydbio.2005.04.013 · Cited by 173 · see citing works
“Group B1 Sox genes are expressed by progenitor cells throughout the developing central nervous system (CNS) (Collignon et al., 1996; Brunelli et al., 2003; Uchikawa et al., 1999; Wood and Episkopou, 1999 ).”
BackgroundSox1 acts through multiple independent pathways to promote neurogenesis
Kan, L., Israsena, N., Zhang, Z., Hu, M., Zhao, L.-R., Jalali, A., Sahni, V., & Kessler, J. A. (2004) · Developmental Biology · doi:10.1016/j.ydbio.2004.02.005 · Cited by 171 · see citing works
“Sox2 and Sox3 begin to be expressed at preimplantation and epiblast stages, respectively, and later become restricted to the neuroepithelium (Collignon et al., 1996; Wood and Episkopou, 1999).”
BackgroundFukuda, H., Takahashi, J. B., Watanabe, K., Hayashi, H., Morizane, A., Koyanagi, M., Sasai, Y., & Hashimoto, N. (2005) · Stem Cells · doi:10.1634/stemcells.2005-0137 · Cited by 167 · see citing works
Okuda, Y., Yoda, H., Uchikawa, M., Furutani‐Seiki, M., Takeda, H., Kondoh, H., & Kamachi, Y. (2006) · Developmental Dynamics · doi:10.1002/dvdy.20678 · Cited by 166 · see citing works
“In amniotes, the group B1 Sox genes, Sox1, Sox2, and Sox3, are widely expressed in the neural primordium of early embryos (Streit et al., 1997; Wood and Episkopou, 1999), and at later developmental stages in the ventricular layer of the CNS, where they function to maintain neural progenitor…”
BackgroundInfluentialKawauchi, S., Shou, J., Santos, R., Hébert, J. M., McConnell, S. K., Mason, I. J., & Calof, A. L. (2005) · Development · doi:10.1242/dev.02143 · Cited by 163 · see citing works
“…cells once the definitive OE structure has been established (Beites et al., 2005; Ellis et al., 2004; Graham et al., 2003; Kawauchi et al., 2004; Wood and Episkopou, 1999), we performed two sets of experiments to try to determine if a subpopulation of the Fgf8expressing cells in the NP are…”
MethodStevanović, M. J., Drakulić, D., Lazic, A., Ninković, D. S., Schwirtlich, M., & Mojsin, M. M. (2021) · Frontiers in Molecular Neuroscience · doi:10.3389/fnmol.2021.654031 · Cited by 158 · see citing works
“Further research has shown that SOX genes encode the family of diverse and well conserved TFs. Based on the structure, expression profiles, as well as the similarity between the proteins they encode, human SOX genes are divided into 8 groups, A to H (Table 1), with group B being further split into subgroups B1 (SOX1,…”
BackgroundInfluentialCatena, R., Tiveron, C., Ronchi, A. E., Porta, S., Ferri, L., Tatangelo, L., Cavallaro, M., Favaro, R., Ottolenghi, S., Reinbold, R. A., et al. (2004) · Journal of Biological Chemistry · doi:10.1074/jbc.m405514200 · Cited by 160 · see citing works
Nervous System Regionalization Entails Axial Allocation before Neural Differentiation
Metzis, V., Steinhauser, S., Pakanavicius, E., Gouti, M., Stamataki, D., Ivanovitch, K., Watson, T., Rayón, T., Mousavy-Gharavy, S. N., Lovell-Badge, R. H., et al. (2018) · Cell · doi:10.1016/j.cell.2018.09.040 · Cited by 153 · see citing works
“Motif enrichment predicted SoxB1 TF motifs (SOX1/2/3) in all three NPs (Figures S3A–S3C), consistent with their expression throughout the neuraxis (Kamachi andKondoh, 2013; Wood and Episkopou, 1999).”
BackgroundPevny, L., & Rao, M. S. (2003) · Trends in Neurosciences · doi:10.1016/s0166-2236(03)00169-3 · Cited by 159 · see citing works
Fgf receptor signaling plays a role in lens induction
Faber, S. C., Dimanlig, P. V., Makarenkova, H. P., Shirke, S., Ko, K. A., & Lang, R. A. (2001) · Development · doi:10.1242/dev.128.22.4425 · Cited by 159 · see citing works
Al-Mayhani, T. M. F., Ball, S. L. R., Zhao, J., Fawcett, J. W., Ichimura, K., Collins, P. V., & Watts, C. (2008) · Journal of Neuroscience Methods · doi:10.1016/j.jneumeth.2008.07.022 · Cited by 157 · see citing works
Mellough, C., Collin, J., Khazim, M., White, K., Sernagor, E., STEEL, D. H., & Lako, M. (2015) · Stem Cells · doi:10.1002/stem.2023 · Cited by 155 · see citing works
Rodríguez, T. A., Srinivas, S., Clements, M., Smith, J. C., & Beddington, R. S. P. (2005) · Development · doi:10.1242/dev.01847 · Cited by 154 · see citing works
“D ev el op m en t 2519AP patterning by the extra-embryonic ectoderm 2004), or, alternatively, is essential for maintaining the levels of Nodal signalling in the epiblast that are required for AVE migration to occur (Norris et al., 2002; Yamamoto et al., 2004).”
BackgroundMammalian sex—Origin and evolution of the Y chromosome and SRY
Waters, P. D., Wallis, M. C., & Graves, J. A. M. (2007) · Seminars in Cell and Developmental Biology · doi:10.1016/j.semcdb.2007.02.007 · Cited by 152 · see citing works
“Mouse Sox3 is first expressed in the developing brain and coninues throughout the development of brain and central nervous ystem (CNS) [93,94].”
Molecular basis of embryonic stem cell self-renewal: from signaling pathways to pluripotency network
Huang, G., Ye, S.-D., Zhou, X., Liu, D.-H., & Ying, Q. (2015) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-015-1833-2 · Cited by 153 · see citing works
“Sox2-null embryos die immediately after implantation [30, 31].”
Essential Role of Sox2 for the Establishment and Maintenance of the Germ Cell Line
Campolo, F., Gori, M., Favaro, R., Nicolis, S., Pellegrini, M., Botti, F., Rossi, P., Jannini, E., & Dolci, S. (2013) · Stem Cells · doi:10.1002/stem.1392 · Cited by 132 · see citing works
Chung, S., Shin, B., Hedlund, E., Pruszak, J., Ferree, A. W., Kang, U. J., Ole Isacson, A., & Kim, K. (2006) · Journal of Neurochemistry · doi:10.1111/j.1471-4159.2006.03841.x · Cited by 142 · see citing works
“…and the epiblast stage, respectively (Pevny et al. 1998), the onset of Sox1 expression correlates with the formation of neural plate, and its expression is down-regulated as neural cells exit the cell cycle and differentiate (Uwanogho et al. 1995; Pevny et al. 1998; Wood and Episkopou 1999).”
BackgroundTsukamoto, T., Inada, K., Tanaka, H., Mizoshita, T., Mihara, M., Ushijima, T., Yamamura, Y., Nakamura, S., & Tatematsu, M. (2004) · Journal of Cancer Research and Clinical Oncology · doi:10.1007/s00432-003-0519-6 · Cited by 140 · see citing works
“Those of group B1, including Sox1, Sox2, and Sox3, are important for gut development in mice (Wood and Episkopou 1999).”
BackgroundSchlosser, G. (2005) · Journal of Experimental Zoology Part B Molecular and Developmental Evolution · doi:10.1002/jez.b.21055 · Cited by 140 · see citing works
Nat, R., Nilbratt, M., Narkilahti, S., Winblad, B. G., Hovatta, O., & Nordberg, A. K. (2006) · Glia · doi:10.1002/glia.20463 · Cited by 139 · see citing works
“Pax6, nestin, and Sox1 are specific markers for this population (Wood and Episkopou, 1999).”
BackgroundMapping gene regulatory circuitry of Pax6 during neurogenesis
Thakurela, S., Tiwari, N., Schick, S., Garding, A., Ivánek, R., Berninger, B., & Tiwari, V. K. (2016) · Cell Discovery · doi:10.1038/celldisc.2015.45 · Cited by 131 · see citing works
Driver, E. C., & Kelley, M. W. (2020) · Development · doi:10.1242/dev.162263 · Cited by 139 · see citing works
“0, including in cells that give rise to the four non-prosensory regions of the mature cochlear duct (Gu et al., 2016; Mak et al., 2009; Steevens et al., 2019; Wood and Episkopou, 1999).”
BackgroundSoxB transcription factors specify neuroectodermal lineage choice in ES cells
Zhao, S., Nichols, J., Smith, A. G., & Li, M. (2004) · Molecular and Cellular Neuroscience · doi:10.1016/j.mcn.2004.08.002 · Cited by 137 · see citing works
Adachi, K., Nikaido, I., Ohta, H., Ohtsuka, S., Ura, H., Kadota, M., Wakayama, T., Ueda, H., & Niwa, H. (2013) · Molecules and Cells · doi:10.1016/j.molcel.2013.09.002 · Cited by 143 · see citing works
Hutton, S. R., & Pevny, L. (2011) · Developmental Biology · doi:10.1016/j.ydbio.2011.01.015 · Cited by 130 · see citing works
“Sox3, a SOXB1 homologue which is coexpressed with Sox2 in neocortical progenitor cells (Collignon et al., 1996; Uwanogho et al., 1995; Wood and Episkopou, 1999), shows a similarly dynamic regulation and its levels directly correlate with those of Sox2 (Fig.”
BackgroundShimozaki, K., Nakashima, K., Niwa, H., & Taga, T. (2003) · Development · doi:10.1242/dev.00476 · Cited by 134 · see citing works
“…next examined the expression of neural and non-neural marker genes in ZHTc6 cells described above cultured with or without Tc. Sox2 is one of the earliest known transcription factors to be expressed in ICM and its expression continues throughout neural tube development (Wood and Episkopou, 1999).”
BackgroundOverton, P. M., Meadows, L. A., Urban, J., & Russell, S. R. H. (2002) · Development · doi:10.1242/dev.129.18.4219 · Cited by 131 · see citing works
Sox2 Induces Neuronal Formation in the Developing Mammalian Cochlea
Puligilla, C., Dabdoub, A., Brenowitz, S. D., & Kelley, M. W. (2010) · Journal of Neuroscience · doi:10.1523/jneurosci.3852-09.2010 · Cited by 130 · see citing works
“In particular, members of the SoxB1 group (Sox1, Sox2, Sox3) of high-mobility-group type (HMG) box domain transcription factors have been shown to play a role in this transition in other systems (Collignon et al., 1996; Pevny et al., 1998; Wood and Episkopou, 1999).”
BackgroundGerm layer induction from embryonic stem cells
Gadue, P. J., Huber, T. L., Nostro, M. C., Kattman, S. J., & Keller, G. M. (2005) · Experimental Hematology · doi:10.1016/j.exphem.2005.06.009 · Cited by 129 · see citing works
“[85] targeted GFP to the sox1 locus, a gene expressed in the earliest stages of neuroectoderm commitment [108].”
BackgroundNeural stem cells: involvement in adult neurogenesis and CNS repair
Okano, H., & Sawamoto, K. (2008) · Philosophical Transactions of the Royal Society B Biological Sciences · doi:10.1098/rstb.2008.2264 · Cited by 130 · see citing works
“…Okano 1997; Kaneko et al. 2000; Okano et al. 2002, 2005), nestin (Hockfield & McKay 1985; Lendahl et al. 1990) and Sox proteins (Pevny et al. 1998; Wood & Episkopou 1999)), development of selective culture methods (clonal (Qian et al. 2000) and highdensity (Palmer et al. 1999) monolayer cultures…”
BackgroundNetwork plasticity of pluripotency transcription factors in embryonic stem cells
Filipczyk, A. A., Marr, C., Hastreiter, S., Feigelman, J. S., Schwarzfischer, M., Hoppe, P. S., Loeffler, D., Kokkaliaris, K. D., Endele, M., Schauberger, B., et al. (2015) · Nature Cell Biology · doi:10.1038/ncb3237 · Cited by 121 · see citing works
Sox2/Oct4: A delicately balanced partnership in pluripotent stem cells and embryogenesis
Rizzino, A., & Wuebben, E. L. (2016) · Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms · doi:10.1016/j.bbagrm.2016.03.006 · Cited by 128 · see citing works
- Authors
- Wood, H. B., & Episkopou, V.
- Affiliations
- Hammersmith Hospital (GB)
- Corresponding author
- Episkopou, Vasso
- Venue
- Mechanisms of Development
- Publisher
- Elsevier BV
- Published
- 1999-08
- Volume
- 86
- Issue
- 1-2
- Pages
- 197-201
- ISSN
- 0925-4773 (ISSN-L)
- OpenAlex
- W2055664397
- Cited by
- 550
- References
- 16
- Citations by year
- 2026: 7 · 2025: 10 · 2024: 15 · 2023: 5 · 2022: 17 · 2021: 16 · 2020: 11 · 2019: 15 · 2018: 18 · 2017: 23 · 2016: 14 · 2015: 31 · 2014: 29 · 2013: 24 · 2012: 30
- FWCI
- 2.61
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Mechanisms of Development (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Medical Research Council
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
- Keywords
- SOX1, SOX2, SOX3, gene expression, mouse embryo, gastrulation, neural plate, neural fold, somitogenesis, primitive streak, chorion, extraembryonic ectoderm, neuroectoderm
- MeSH terms
- Animals, Central Nervous System, DNA-Binding Proteins, Ectoderm, Embryo, Mammalian, Gastrula, High Mobility Group Proteins, Nuclear Proteins, Rhombencephalon, Transcription Factors, Gene Expression Regulation, Developmental, HMGB Proteins, Mice, SOXB1 Transcription Factors
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
