A novel spreadsheet method for calculating the free serum concentrations of testosterone, dihydrotestosterone, estradiol, estrone and cortisol: With illustrative examples from male and female populations

Mazer, Norman A.

doi:10.1016/j.steroids.2009.01.008

Cited by 189Hormonal and reproductive studiesAdrenal Hormones and DisordersEstrogen and related hormone effects

A novel spreadsheet method for calculating the free serum concentrations of testosterone, dihydrotestosterone, estradiol, estrone and cortisol: With illustrative examples from male and female populations

Mazer, Norman A.·2009·Steroids

DOI 10.1016/j.steroids.2009.01.008

Open this paper in CoChat — free account

Abstract

In humans, testosterone (T), dihydrotestosterone (DHT), estradiol (E2), estrone (E1) and cortisol (C) bind to the serum proteins sex hormone-binding globulin (SHBG), albumin (Alb) and corticosteroid-binding globulin (CBG). Equilibrium dialysis is considered to be the "gold standard" for measuring the free concentrations of these steroids but is technically difficult and not widely available. Based on a mathematical model of the 5-ligand/3-protein binding equilibria, we developed a novel spreadsheet method for calculating the free and bioavailable (free+Alb-bound) concentrations of each steroid in terms of the total steroid and protein concentrations. The model uses 15 association constants K(SHBG-X), K(Alb-X), and K(CBG-X) (X=T, DHT, E2, E1 and C) that have been estimated from a systematic review of published binding studies. The computation of the free and bioavailable concentrations uses an iterative numerical method that can be readily programmed on a spreadsheet. The method is illustrated with six examples corresponding to young men (YM), old men (OM), obese men (Ob M), young women (YM), pregnant women in the 3rd trimester (Preg T3) and oophorectomized women on oral conjugated equine estrogens (CEE). The resulting free hormone concentrations for YM and YW fall within the normal references ranges obtained by equilibrium dialysis for all five hormones. The model also accounts for the competitive binding effects of high estrogen levels on the free T levels in Preg T3. This novel spreadsheet method provides a "user-friendly" approach for estimating the free concentrations of circulating sex hormones and cortisol in men and women.

Integrity signals

Registry

Crossref · 10.1016/j.steroids.2009.01.008

Cites

53 of 53 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Testosterone has the second highest association constant for SHBG (Ka 1-2 × 10^9 M−1 s−1), whereas DHT has the highest Ka for SHBG (2.69 × 10^9 M−1 s−1).
“Among steroid hormones, T has the second highest association constant (Ka), valued at 1–2 × 109 M−1 s−1, whereas DHT has the highest Ka for SHBG (2.69 × 109 M−1 s−1) [16].”
From Role of sex hormone-binding globulin in the free hormone hypothesis and the relevance of free testosterone in androgen physiology (2022) · cites this paper as [16]
The first category of free T equations was derived from the general law of mass action, with association constants for albumin and SHBG obtained from in vitro experiments by Scatchard analysis.
“First, several equations were derived from the general law of mass action. Association constants for albumin and SHBG used in these formulas were obtained from in vitro experiments by linearizing data through Scatchard analysis [16, 208, 212].”
From Role of sex hormone-binding globulin in the free hormone hypothesis and the relevance of free testosterone in androgen physiology (2022) · cites this paper as [16]
Free T algorithms can be divided into three categories: linear mass action, non-linear allosteric, and empirical bootstrap.
“A wide spectrum of algorithms for free T exists, which can broadly be divided into three categories (Table 6).”
From Role of sex hormone-binding globulin in the free hormone hypothesis and the relevance of free testosterone in androgen physiology (2022) · cites this paper as [16]
Circulating testosterone is predominantly bound to sex hormone binding globulin and albumin, with small percentages freely circulating or bound to corticosteroid binding globulin.
“Circulating T is predominantly bound to sex hormone binding globulin (SHBG) and albumin, with small percentages freely circulating or associated with corticosteroid binding globulin.”
From Challenges in Testosterone Measurement, Data Interpretation, and Methodological Appraisal of Interventional Trials (2016) · cites this paper as [10]
Testosterone levels were measured by a radioimmunoassay validated against liquid chromatography tandem mass spectrometry.
“Testosterone levels were measured by a radioimmunoassay that has been validated against liquid chromatography tandem mass spectrometry (13) and has a sensitivity of 10 ng/dL.”
From Testosterone induces erythrocytosis via increased erythropoietin and suppressed hepcidin: evidence for a new erythropoietin/hemoglobin set point (2014) · cites this paper as [13]

A Critical Evaluation of Simple Methods for the Estimation of Free Testosterone in Serum
Vermeulen, A., Verdonck, L., & Kaufman, J. M. (1999) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jcem.84.10.6079 · Cited by 3,824 · see citing works
Transport of steroid hormones: binding of 21 endogenous steroids to both testosterone-binding globulin and corticosteroid-binding globulin in human plasma
DUNN, J. F., NISULA, B. C., & RODBARD, D. (1981) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jcem-53-1-58 · Cited by 1,238 · see citing works
Sex‐hormone binding-globulin
ANDERSON, D. C. (1974) · Clinical Endocrinology · doi:10.1111/j.1365-2265.1974.tb03298.x · Cited by 1,127 · see citing works
Calculation of free and bound fractions of testosterone and estradiol-17β to human plasma proteins at body temperature
Södergard, R., Bäckström, T., Shanbhag, V., & Carstensen, H. (1982) · Journal of Steroid Biochemistry · doi:10.1016/0022-4731(82)90038-3 · Cited by 1,080 · see citing works
The Free Hormone Hypothesis: A Physiologically Based Mathematical Model*
MENDEL, C. M. (1989) · Endocrine Reviews · doi:10.1210/edrv-10-3-232 · Cited by 961 · see citing works
Transdermal testosterone treatment in women with impaired sexual function after oophorectomy.
Shifren, J. L., Braunstein, G. D., Simon, J. A., Casson, P. R., Buster, J. E., Redmond, G. P., Burki, R. E., Ginsburg, E. S., Rosen, R. C., Leiblum, S. R., et al. (2000) · New England Journal of Medicine · doi:10.1056/nejm200009073431002 · Cited by 954 · see citing works
Self-citation
HPA axis responses to laboratory psychosocial stress in healthy elderly adults, younger adults, and children: impact of age and gender
Kudielka, B. M., Buske-Kirschbaum, A., Hellhammer, D. H., & Kirschbaum, C. (2004) · Psychoneuroendocrinology · doi:10.1016/s0306-4530(02)00146-4 · Cited by 851 · see citing works
Leptin and Androgens in Male Obesity: Evidence for Leptin Contribution to Reduced Androgen Levels
Isidori, A. M., Caprio, M., Strollo, F., Moretti, C., Frajese, G., Isidori, A., & Fabbri, A. (1999) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jcem.84.10.6082 · Cited by 560 · see citing works
Effects of transdermal testosterone on bone and muscle in older men with low bioavailable testosterone levels
Kenny, A. M., Prestwood, K. M., Gruman, C. A., Marcello, K. M., & Raisz, L. G. (2001) · The Journals of Gerontology Series A: Biological Sciences and Medical Sciences · doi:10.1093/gerona/56.5.m266 · Cited by 456 · see citing works
Influence of some biological indexes on sex hormone-binding globulin and androgen levels in aging or obese males
Vermeulen, A. (1996) · Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jc.81.5.1821 · Cited by 455 · see citing works
The Apparent Free Testosterone Concentration, An Index of Androgenicity
Vermeulen, A., Stoica, T., & Verdonck, L. (1971) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jcem-33-5-759 · Cited by 388 · see citing works
Capacity of the Testosterone-Binding Globulin in Human Plasma and Influence of Specific Binding of Testosterone on Its Metabolic Clearance Rate
Vermeulen, A., Verdonck, L., Straeten, M. V. D., & ORIE, N. (1969) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jcem-29-11-1470 · Cited by 385 · see citing works
Measurement of Free Hormones in Blood
Ekins, R. P. (1990) · Endocrine Reviews · doi:10.1210/edrv-11-1-5 · Cited by 308 · see citing works
Measurement of Free Testosterone in Normal Women and Women with Androgen Deficiency: Comparison of Methods
Miller, K. K., Rosner, W., Lee, H., Hier, J., Sesmilo, G., Schoenfeld, D., Neubauer, G., & Klibanski, A. (2004) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jc.2003-030680 · Cited by 301 · see citing works
Sex hormone-binding globulin mediates steroid hormone signal transduction at the plasma membrane
Rosner, W., Hryb, D. J., Khan, M., Nakhla, A. M., & Romas, N. A. (1999) · The Journal of Steroid Biochemistry and Molecular Biology · doi:10.1016/s0960-0760(99)00070-9 · Cited by 195 · see citing works
Steroid hormone levels in pregnancy and 1 year postpartum using isotope dilution tandem mass spectrometry
Soldin, O. P., Guo, T., Weiderpass, E., Tractenberg, R. E., Hilakivi‐Clarke, L., & Soldin, S. J. (2005) · Fertility and Sterility · doi:10.1016/j.fertnstert.2005.02.045 · Cited by 186 · see citing works
Empirical estimation of free testosterone from testosterone and sex hormone-binding globulin immunoassays
Ly, L. P., & Handelsman, D. J. (2005) · European Journal of Endocrinology · doi:10.1530/eje.1.01844 · Cited by 180 · see citing works
Isolation and characterization of the testosterone-estradiol-binding globulin from human plasma. Use of a novel affinity column
Rosner, W., & Smith, R. N. (1975) · Biochemistry · doi:10.1021/bi00693a006 · Cited by 154 · see citing works
The plasma sex steroid binding protein (SBP or SHBG). A critical review of recent developments on the structure, molecular biology and function
Pétra, P. H. (1991) · The Journal of Steroid Biochemistry and Molecular Biology · doi:10.1016/0960-0760(91)90299-k · Cited by 147 · see citing works
Mathematical theory of cross-reactive radioimmunoassay and ligand-binding systems at equilibrium
Feldman, H. A., Rodbard, D., & Levine, D. M. (1972) · Analytical Biochemistry · doi:10.1016/0003-2697(72)90216-3 · Cited by 143 · see citing works
Errors in the Measurement of Plasma Free Testosterone 1
Rosner, W. (1997) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jcem.82.6.9999 · Cited by 140 · see citing works
The Free Hormone Hypothesis Distinction from the Free Hormone Transport Hypothesis
Mendel, C. M. (1992) · Journal of Andrology · doi:10.1002/j.1939-4640.1992.tb01639.x · Cited by 128 · see citing works
A mathematical comparison of techniques to predict biologically available testosterone in a cohort of 1072 men
Morris, P., Malkin, C., Channer, K., & Jones, T. (2004) · European Journal of Endocrinology · doi:10.1530/eje.0.1510241 · Cited by 122 · see citing works
Reversal of the hypogonadotropic hypogonadism of obese men by administration of the aromatase inhibitor testolactone
Zumoff, B., Miller, L. K., & Strain, G. W. (2003) · Metabolism · doi:10.1016/s0026-0495(03)00186-0 · Cited by 118 · see citing works
Enhanced cortisol production rates, free cortisol, and 11β-HSD-1 expression correlate with visceral fat and insulin resistance in men: effect of weight loss
Purnell, J. Q., Kahn, S. E., Samuels, M. H., Brandon, D., Loriaux, D. L., & Brunzell, J. D. (2009) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.90769.2008 · Cited by 112 · see citing works
Associations of Sex-Hormone-Binding Globulin (SHBG) with Non-SHBG-Bound Levels of Testosterone and Estradiol in Independently Living Men
de Ronde, W., van der Schouw, Y. T., Muller, M., Grobbee, D. E., Gooren, L. J., Pols, H. A. P., & de Jong, F. H. (2005) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jc.2004-0422 · Cited by 97 · see citing works
Steroid-protein interactions. Human corticosteroid binding globulin: some physicochemical properties and binding specificity
Mickelson, K. E., Forsthoefel, J., & Westphal, U. (1981) · Biochemistry · doi:10.1021/bi00524a047 · Cited by 95 · see citing works
Estradiol-Testosterone Binding Interactions and Free Plasma Estradiol under Physiological Conditions*
Moll, G., ROSENFIEL, R. L., & Helke, J. (1981) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jcem-52-5-868 · Cited by 92 · see citing works
Cortisol Levels, Binding, and Properties of Corticosteroid-Binding Globulin in the Serum of Primates*
Klosterman, L. L., Murai, J. T., & Siiteri, P. K. (1986) · Endocrinology · doi:10.1210/endo-118-1-424 · Cited by 89 · see citing works
A randomized, open-label, crossover study comparing the effects of oral versus transdermal estrogen therapy on serum androgens, thyroid hormones, and adrenal hormones in naturally menopausal women
Shifren, J. L., Desindes, S., McIlwain, M., Doros, G., & Mazer, N. A. (2007) · Menopause · doi:10.1097/gme.0b013e31803867a · Cited by 89 · see citing works
Self-citation
Interspecies variations of corticosteroid-binding globulin parameters
Gayrard, V., Alvinerie, M., & Toutain, P. (1996) · Domestic Animal Endocrinology · doi:10.1016/0739-7240(95)00042-9 · Cited by 83 · see citing works
Transport of protein-bound steroid hormones into liver in vivo.
Pardridge, W. M., & Mietus, L. J. (1979) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.1979.237.4.e367 · Cited by 79 · see citing works
Plasma Cortisol Transport and Primate Evolution
Pugeat, M., Chrousos, G. P., Nisula, B. C., Loriaux, D. L., Brandon, D. D., & LIPSETT, M. B. (1984) · Endocrinology · doi:10.1210/endo-115-1-357 · Cited by 79 · see citing works
The Endocrine Function of the Human Testis
(1973) · Elsevier eBooks · doi:10.1016/c2013-0-10910-5 · Cited by 75 · see citing works
Resolution of the Human Sex Hormone-binding Globulin Dimer Interface and Evidence for Two Steroid-binding Sites per Homodimer
Avvakumov, G. V., Grishkovskaya, I., Muller, Y. A., & Hammond, G. L. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.m106274200 · Cited by 74 · see citing works
Sex hormone-binding globulin/androgen-binding protein: Steroid-binding and dimerization domains
Hammond, G. L., & Bocchinfuso, W. P. (1995) · The Journal of Steroid Biochemistry and Molecular Biology · doi:10.1016/0960-0760(95)00110-l · Cited by 71 · see citing works
Interaction of Estrogen Therapy and Thyroid Hormone Replacement in Postmenopausal Women
Mazer, N. A. (2004) · Thyroid · doi:10.1089/105072504323024561 · Cited by 68 · see citing works
Self-citation
Longitudinal study of maternal plasma bioavailable testosterone and androstanediol glucuronide levels during pregnancy
Kerlan, V., Nahoul, K., Le Martelot, M., & Bercovici, J. (1994) · Clinical Endocrinology · doi:10.1111/j.1365-2265.1994.tb02478.x · Cited by 66 · see citing works
Maternal hormone levels among populations at high and low risk of testicular germ cell cancer
Zhang, Y., Graubard, B. I., Klebanoff, M. A., Ronckers, C. M., Stanczyk, F. Z., Longnecker, M. P., & McGlynn, K. A. (2005) · British Journal of Cancer · doi:10.1038/sj.bjc.6602545 · Cited by 45 · see citing works
An updated steroid benchmark set and its application in the discovery of novel nanomolar ligands of sex hormone-binding globulin
Cherkasov, A., Ban, F., Santos-Filho, O., Thorsteinson, N., Fallahi, M., & Hammond, G. L. (2008) · Journal of Medicinal Chemistry · doi:10.1021/jm7011485 · Cited by 42 · see citing works
INTERACTION BETWEEN HYDROCORTISONE-4-C14OR PROGESTERONE-4-C14AND SERUM ALBUMIN AS DEMONSTRATED BY ULTRACENTRIFUGATION AND ELECTROPHORESIS
Westphal, U. (1955) · Endocrinology · doi:10.1210/endo-57-4-456 · Cited by 37 · see citing works
Corticosteroid-binding globulin affects the relationship between circulating adiponectin and cortisol in men and women
Fernández‐Real, J. M., PUGEAT, M. M., López‐Bermejo, A., Bornet, H., & Ricart, W. (2005) · Metabolism · doi:10.1016/j.metabol.2004.11.015 · Cited by 32 · see citing works
Interactions of steroids with human plasma proteins
Slaunwhite, W. R., Rosenthal, H. E., & Sandberg, A. A. (1963) · Archives of Biochemistry and Biophysics · doi:10.1016/0003-9861(63)90116-4 · Cited by 27 · see citing works
THE PHYSICAL STATE OF TESTOSTERONE IN PLASMA
Vermeulen, A. (1973) · Elsevier eBooks · doi:10.1016/b978-0-12-380101-2.50013-x · Cited by 26 · see citing works
Corticosteroid-binding globulin
Westphal, U. (1983) · Molecular and Cellular Biochemistry · doi:10.1007/bf00673709 · Cited by 25 · see citing works
Validity of the calculation of non-sex hormone-binding globulin-bound estradiol from total testosterone, total estradiol and sex hormone-binding globulin concentrations in human serum
Belgorosky, A., Escobar, M. E., & Rivarola, M. A. (1987) · Journal of Steroid Biochemistry · doi:10.1016/0022-4731(87)91061-2 · Cited by 25 · see citing works
Binding Analysis of 1α- and 17α-Dihydrotestosterone Derivatives to Homodimeric Sex Hormone-Binding Globulin
Metzger, J., Schnitzbauer, A. A., Meyer, M., Söder, M., Cuilleron, C. Y., Hauptmann, H., Huber, E., & Luppa, P. B. (2003) · Biochemistry · doi:10.1021/bi035269k · Cited by 25 · see citing works
The temperature dependence of the binding of 5α- dihydrotestosterone, testosterone and estradiol to the sex hormone globulin (SHBG) of human plasma
Shanbhag, V. P., & Södergård, R. (1986) · Journal of Steroid Biochemistry · doi:10.1016/0022-4731(86)90118-4 · Cited by 24 · see citing works
Circulating Progesterone and Obesity in Men
Blanchette, S., Marceau, P., Biron, S., Brochu, G., & Tchernof, A. (2006) · Hormone and Metabolic Research · doi:10.1055/s-2006-925392 · Cited by 22 · see citing works
BINDING OF 5α-ANDROSTANE-3α,17β-DIOL TO HUMAN PLASMA PROTEINS
CLARK, A. F., & BIRD, C. E. (1973) · Journal of Endocrinology · doi:10.1677/joe.0.0570289 · Cited by 14 · see citing works
San Juan Capistrano
Jong, D. A., & James, A. (1977) · Pro Rege · Cited by 8 · see citing works
Steady-state pharmacokinetics of conjugated equine estrogens in healthy, postmenopausal women.
Mayer, P. R., Tse, S. Y. H., Sendi, M., Bourg, D., & Morrison, D. (2008) · PubMed · Cited by 7 · see citing works
Circulating concentrations of hemostatic factors and two "steroid sensitive proteins" during oral hormone replacement therapy in women with coronary heart disease
Pripp, U., Schenck-Gustafsson, K. G., Landgren, B.-M., & Carlström, K. (2004) · Scandinavian Journal of Clinical and Laboratory Investigation · doi:10.1080/00365510410002995 · Cited by 6 · see citing works

Flagged first, then by citations

Sorted by citations

Adverse Events Associated with Testosterone Administration
Basaria, S., Coviello, A. D., Travison, T. G., Storer, T. W., Farwell, W. R., Jette, A. M., Eder, R., Tennstedt, S., Ulloor, J., Zhang, A., et al. (2010) · New England Journal of Medicine · doi:10.1056/nejmoa1000485 · Cited by 1,494 · see citing works
Self-citation
Testosterone induces erythrocytosis via increased erythropoietin and suppressed hepcidin: evidence for a new erythropoietin/hemoglobin set point
Bachman, E., Travison, T. G., Basaria, S., Davda, M. N., Guo, W., Li, M., Connor Westfall, J., Bae, H., Gordeuk, V., & Bhasin, S. (2014) · The Journals of Gerontology Series A: Biological Sciences and Medical Sciences · doi:10.1093/gerona/glt154 · Cited by 426 · see citing works
Alterations in Gut Microbiome Composition and Barrier Function Are Associated with Reproductive and Metabolic Defects in Women with Polycystic Ovary Syndrome (PCOS): A Pilot Study
Lindheim, L., Bashir, M., Münzker, J., Trummer, C., Zachhuber, V., Leber, B., Horvath, A., Pieber, T. R., Gorkiewicz, G., Stadlbauer, V., et al. (2017) · PLOS ONE · doi:10.1371/journal.pone.0168390 · Cited by 416 · see citing works
“Free testosterone and free DHT were calculated from total testosterone/DHT and SHBG according to Mazer, assuming a blood albumin concentration of 6.2 μmol/l [28].”
Influential
A Reappraisal of Testosterone’s Binding in Circulation: Physiological and Clinical Implications
Goldman, A. L., Bhasin, S., Wu, F. C. W., Krishna, M., Matsumoto, A. M., & Jasuja, R. (2017) · Endocrine Reviews · doi:10.1210/er.2017-00025 · Cited by 414 · see citing works
Androgen therapy in women: a reappraisal: an Endocrine Society Clinical Practice Guideline
Wierman, M. E., Arlt, W., Basson, R., Davis, S. R., Miller, K. K., Murad, M. H., Rosner, W., & Santoro, N. (2014) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jc.2014-2260 · Cited by 363 · see citing works
Reference Ranges for Testosterone in Men Generated Using Liquid Chromatography Tandem Mass Spectrometry in a Community-Based Sample of Healthy Nonobese Young Men in the Framingham Heart Study and Applied to Three Geographically Distinct Cohorts
Bhasin, S., Pencina, M., Jasuja, G. K., Travison, T. G., Coviello, A., Orwoll, E., Wang, P. Y., Nielson, C., Wu, F., Tajar, A., et al. (2011) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jc.2010-3012 · Cited by 355 · see citing works
Male hypogonadism
Basaria, S. (2014) · The Lancet · doi:10.1016/s0140-6736(13)61126-5 · Cited by 328 · see citing works
Effects of Testosterone Administration for 3 Years on Subclinical Atherosclerosis Progression in Older Men With Low or Low-Normal Testosterone Levels: A Randomized Clinical Trial
Basaria, S., Harman, S. M., Travison, T. G., Hodis, H., Tsitouras, P., Budoff, M., Pencina, K. M., Vita, J., Dzekov, C., Mazer, N. A., et al. (2015) · JAMA · doi:10.1001/jama.2015.8881 · Cited by 268 · see citing works
Self-citation
Liquid chromatography tandem mass spectrometry for analysis of steroids in clinical laboratories
Kushnir, M. M., Rockwood, A. L., Roberts, W. L., Yue, B., Bergquist, J., & Meikle, A. W. (2010) · Clinical Biochemistry · doi:10.1016/j.clinbiochem.2010.07.008 · Cited by 226 · see citing works
Genetic determinants of serum testosterone concentrations in men
Ohlsson, C., Wallaschofski, H., Lunetta, K. L., Stolk, L., Perry, J. R. B., Koster, A., Petersen, A.-K., Eriksson, J., Lehtimäki, T., Huhtaniemi, I. T., et al. (2011) · PLoS Genetics · doi:10.1371/journal.pgen.1002313 · Cited by 224 · see citing works
Sex-specific estrogen levels and reference intervals from infancy to late adulthood determined by LC-MS/MS
Frederiksen, H., Johannsen, T. H., Andersen, S. E., Albrethsen, J., Landersoe, S. K., Petersen, J. H., Andersen, A. N., Vestergaard, E. T., Schorring, M. E., Linneberg, A., et al. (2020) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/clinem/dgz196 · Cited by 211 · see citing works
Effects of Polycystic Ovary Syndrome (PCOS), Sex Hormones, and Obesity on Circulating miRNA-21, miRNA-27b, miRNA-103, and miRNA-155 Expression
Murri, M., Insenser, M. R., Fernández‐Durán, E., San‐Millán, J. L., & Escobar-Morreale, H. F. (2013) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jc.2013-2218 · Cited by 188 · see citing works
Influence of sex and hormone status on circulating natriuretic peptides
Lam, C. S. P., Cheng, S., Choong, K., Larson, M. G., Murabito, J. M., Newton-Cheh, C., Bhasin, S., McCabe, E. L., Miller, K. K., Redfield, M. M., et al. (2011) · Journal of the American College of Cardiology · doi:10.1016/j.jacc.2011.03.042 · Cited by 181 · see citing works
Cardiac Natriuretic Peptides, Obesity, and Insulin Resistance: Evidence from Two Community-Based Studies
Khan, A. M., Cheng, S. C., Magnusson, M., Larson, M. G., Newton‐Cheh, C., McCabe, E. L., Coviello, A. D., Florez, J. C., Fox, C. S., Levy, D. A., et al. (2011) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jc.2011-1182 · Cited by 175 · see citing works
Effects of Testosterone Supplementation for 3-Years on Muscle Performance and Physical Function in Older Men
Storer, T. W., Basaria, S., Traustadottir, T., Harman, S. M., Pencina, K., Li, Z., Travison, T. G., Miciek, R., Tsitouras, P., Hally, K., et al. (2016) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jc.2016-2771 · Cited by 174 · see citing works
Clinical Meaningfulness of the Changes in Muscle Performance and Physical Function Associated With Testosterone Administration in Older Men With Mobility Limitation
Travison, T. G., Basaria, S., Storer, T. W., Jette, A. M., Miciek, R., Farwell, W. R., Choong, K., Lakshman, K., Mazer, N. A., Coviello, A. D., et al. (2011) · The Journals of Gerontology Series A: Biological Sciences and Medical Sciences · doi:10.1093/gerona/glr100 · Cited by 160 · see citing works
Self-citation
Free Testosterone Levels Are Associated with Mobility Limitation and Physical Performance in Community-Dwelling Men: The Framingham Offspring Study
Krasnoff, J. B., Basaria, S., Pencina, M. J., Jasuja, G. K., Vasan, R. S., Ulloor, J., Zhang, A., Coviello, A., Kelly-Hayes, M., D'Agostino, R. B., et al. (2010) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jc.2009-2680 · Cited by 152 · see citing works
Effect of Testosterone Supplementation With and Without a Dual 5α-Reductase Inhibitor on Fat-Free Mass in Men With Suppressed Testosterone Production: A Randomized Controlled Trial
Bhasin, S., Travison, T. G., Storer, T. W., Lakshman, K., Kaushik, M., Mazer, N. A., Ngyuen, A.-H., Davda, M. N., Jara, H., Aakil, A., et al. (2012) · JAMA · doi:10.1001/jama.2012.227 · Cited by 152 · see citing works
Self-citation
The Safety, Pharmacokinetics, and Effects of LGD-4033, a Novel Nonsteroidal Oral, Selective Androgen Receptor Modulator, in Healthy Young Men
Basaria, S. S., Collins, L. F., Dillon, E. L., Orwoll, K., Storer, T. W., Miciek, R., Ulloor, J., Zhang, A., Eder, R. V., Zientek, H., et al. (2010) · The Journals of Gerontology Series A · doi:10.1093/gerona/gls078 · Cited by 154 · see citing works
Hypogonadism in the Aging Male Diagnosis, Potential Benefits, and Risks of Testosterone Replacement Therapy
Surampudi, P. N., Wang, C., & Swerdloff, R. S. (2012) · International Journal of Endocrinology · doi:10.1155/2012/625434 · Cited by 151 · see citing works
Aging and the Male Reproductive System.
Kaufman, J., Lapauw, B., Mahmoud, A., T'sjoen, G., & Huhtaniemi, I. (2019) · Endocrine reviews · doi:10.1210/er.2018-00178 · Cited by 97 · see citing works
ORIGINAL ARTICLE: Accuracy of calculated free testosterone formulae in men
Ly, L. P., Sartorius, G., Hull, L., Leung, A., Swerdloff, R. S., Wang, C., & Handelsman, D. J. (2010) · Clinical Endocrinology · doi:10.1111/j.1365-2265.2010.03804.x · Cited by 172 · see citing works
Acupuncture for ovulation induction in polycystic ovary syndrome: a randomized controlled trial
Johansson, J., Redman, L. M., Veldhuis, P. P., Sazonova, A., Labrie, F., Holm, G., Johannsson, G., & Stener‐Victorin, E. (2013) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00039.2013 · Cited by 131 · see citing works
Testosterone, Dihydrotestosterone, and Incident Cardiovascular Disease and Mortality in the Cardiovascular Health Study
Shores, M. M., Biggs, M. L., Arnold, A. M., Smith, N. L., Longstreth, W. T., Kizer, J. R., Hirsch, C. H., Cappola, A. R., & Matsumoto, A. M. (2014) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jc.2013-3576 · Cited by 130 · see citing works
Vitamin D deficiency and low ionized calcium are linked with semen quality and sex steroid levels in infertile men
Jensen, M. B., Lawaetz, J. G., Andersson, A., Petersen, J. H., Nordkap, L., Bang, A. K., Ekbom, P., Joensen, U. N., Prætorius, L., Lundstrøm, P., et al. (2016) · Human Reproduction · doi:10.1093/humrep/dew152 · Cited by 122 · see citing works
Differential influence of peripheral and systemic sex steroids on skeletal muscle quality in pre‐ and postmenopausal women
Pöllänen, E., Sipilä, S., Alén, M., Ronkainen, P. H. A., Ankarberg‐Lindgren, C., Puolakka, J. O., Suominen, H., Hämäläinen, E. K., Turpeinen, U., Konttinen, Y. T., et al. (2011) · Aging Cell · doi:10.1111/j.1474-9726.2011.00701.x · Cited by 121 · see citing works
Testosterone but not estradiol level is positively related to muscle strength and physical performance independent of muscle mass: a cross-sectional study in 1489 older men
Auyeung, T. W., Lee, J. S. W., Kwok, T. C. Y., Leung, J. C. S., Ohlsson, C., Vandenput, L., Leung, P., & Woo, J. (2011) · European Journal of Endocrinology · doi:10.1530/eje-10-0952 · Cited by 109 · see citing works
The Effects of Injected Testosterone Dose and Age on the Conversion of Testosterone to Estradiol and Dihydrotestosterone in Young and Older Men
Lakshman, K. M., Kaplan, B., Travison, T. G., Basaria, S. S., Knapp, P. E., Singh, A. B., LaValley, M. P., Mazer, N. A., & Bhasin, S. (2010) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jc.2010-0102 · Cited by 103 · see citing works
Self-citation
Role of sex hormone-binding globulin in the free hormone hypothesis and the relevance of free testosterone in androgen physiology
Narinx, N., David, K., Walravens, J., Vermeersch, P., Claessens, F., Fiers, T., Lapauw, B., Antonio, L., & Vanderschueren, D. (2022) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-022-04562-1 · Cited by 96 · see citing works
Challenges in Testosterone Measurement, Data Interpretation, and Methodological Appraisal of Interventional Trials
Trost, L. W., & Mulhall, J. P. (2016) · The Journal of Sexual Medicine · doi:10.1016/j.jsxm.2016.04.068 · Cited by 98 · see citing works
Diagnosis and treatment of hypogonadism in men
Bhasin, S., & Basaria, S. S. (2011) · Best Practice & Research Clinical Endocrinology & Metabolism · doi:10.1016/j.beem.2010.12.002 · Cited by 89 · see citing works
Non-targeted profiling of circulating microRNAs in women with polycystic ovary syndrome (PCOS): effects of obesity and sex hormones
Murri, M., Insenser, M. R., Fernández‐Durán, E., San‐Millán, J. L., Luque‐Ramírez, M., & Escobar-Morreale, H. F. (2018) · Metabolism · doi:10.1016/j.metabol.2018.01.011 · Cited by 88 · see citing works
Biochemistry of the menopause
Honour, J. W. (2017) · Annals of Clinical Biochemistry International Journal of Laboratory Medicine · doi:10.1177/0004563217739930 · Cited by 84 · see citing works
A multi-step, dynamic allosteric model of testosterone's binding to sex hormone binding globulin
Zakharov, M. N., Bhasin, S., Travison, T. G., Xue, R., Ulloor, J., Vasan, R. S., Carter, E., Wu, F., & Jasuja, R. (2015) · Molecular and Cellular Endocrinology · doi:10.1016/j.mce.2014.09.001 · Cited by 81 · see citing works
Strength Training Induces Muscle Hypertrophy and Functional Gains in Black Prostate Cancer Patients Despite Androgen Deprivation Therapy
Hanson, E. D., Sheaff, A. K., Sood, S., Ma, L., Francis, J. D., Goldberg, A. P., & Hurley, B. F. (2012) · The Journals of Gerontology Series A · doi:10.1093/gerona/gls206 · Cited by 78 · see citing works
Free Testosterone: Pumping up the Tires or Ending the Free Ride?
Handelsman, D. J. (2017) · Endocrine Reviews · doi:10.1210/er.2017-00171 · Cited by 78 · see citing works
Prenatal and adult androgen activities in alcohol dependence
Lenz, B., Mühle, C., Braun, B., Weinland, C., Bouna-Pyrrou, P., Behrens, J., Kubis, S., Mikolaiczik, K., Muschler, M.-R., Saigali, S., et al. (2017) · Acta Psychiatrica Scandinavica · doi:10.1111/acps.12725 · Cited by 74 · see citing works
Sexual dimorphism in adipose tissue function as evidenced by circulating adipokine concentrations in the fasting state and after an oral glucose challenge
Luque‐Ramírez, M., Martínez-García, M. Á., Montes‐Nieto, R., Fernández‐Durán, E., Insenser, M. R., Alpañés, M., & Escobar-Morreale, H. F. (2013) · Human Reproduction · doi:10.1093/humrep/det097 · Cited by 73 · see citing works
Neuroprotective and neurotoxic outcomes of androgens and estrogens in an oxidative stress environment
Duong, P., Tenkorang, M. A. A., Trieu, J., McCuiston, C., Rybalchenko, N., & Cunningham, R. L. (2020) · Biology of Sex Differences · doi:10.1186/s13293-020-0283-1 · Cited by 72 · see citing works
Reproductive Aging in Men
Basaria, S. S. (2013) · Endocrinology and Metabolism Clinics of North America · doi:10.1016/j.ecl.2013.02.012 · Cited by 74 · see citing works
Androgens, health and sexuality in women and men
Wylie, K. R., REES, M. C. P., Hackett, G. I., Anderson, R. A., Bouloux, P.-M. G., Cust, M., Goldmeier, D., Kell, P. D., TERRY, T. R., TRINICK, T. R., et al. (2010) · Maturitas · doi:10.1016/j.maturitas.2010.07.011 · Cited by 72 · see citing works
Effects of aggressive encounters on plasma corticosteroid-binding globulin and its ligands in white-crowned sparrows
Charlier, T. D., Underhill, C., Hammond, G. L., & Soma, K. K. (2009) · Hormones and Behavior · doi:10.1016/j.yhbeh.2009.06.012 · Cited by 71 · see citing works
Maternal and female fetal testosterone levels are associated with maternal age and gestational weight gain
Kallak, T. K., Hellgren, C., Skalkidou, A., Sandelin-Francke, L., Ubhayasekhera, K., Bergquist, J., Axelsson, O., Comasco, E., Campbell, R. E., & Sundström Poromaa, I. (2017) · European Journal of Endocrinology · doi:10.1530/eje-17-0207 · Cited by 70 · see citing works
Adult digit ratio (2D:4D) is not related to umbilical cord androgen or estrogen concentrations, their ratios or net bioactivity
Lawson, L. P., Keelan, J. A., Jamnadass, E. S. L., Maybery, M. T., Hickey, M., & Whitehouse, A. (2015) · Early Human Development · doi:10.1016/j.earlhumdev.2014.12.011 · Cited by 70 · see citing works
Testosterone and dihydrotestosterone and incident ischaemic stroke in men in the C ardiovascular H ealth S tudy
Shores, M. M., Arnold, A. M., Biggs, M. L., Longstreth, W. T., Smith, N. L., Kizer, J. R., Cappola, A. R., Hirsch, C. H., Marck, B. T., & Matsumoto, A. M. (2014) · Clinical Endocrinology · doi:10.1111/cen.12452 · Cited by 71 · see citing works
Reassessing Free-Testosterone Calculation by Liquid Chromatography–Tandem Mass Spectrometry Direct Equilibrium Dialysis
Fiers, T., Wu, F., Moghetti, P., Vanderschueren, D., Lapauw, B., & Kaufman, J.-M. (2018) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jc.2017-02360 · Cited by 70 · see citing works
Accuracy of calculated free testosterone differs between equations and depends on gender and SHBG concentration
Hackbarth, J. S., Hoyne, J. B., Grebe, S. K. G., & Singh, R. (2010) · Steroids · doi:10.1016/j.steroids.2010.08.008 · Cited by 62 · see citing works
Long-Term Testosterone Supplementation in Older Men Attenuates Age-Related Decline in Aerobic Capacity
Traustadóttir, T., Harman, S. M., Tsitouras, P. D., Pencina, K. M., Li, Z., Travison, T. G., Eder, R. V., Miciek, R., McKinnon, J., Woodbury, E. A., et al. (2018) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jc.2017-01902 · Cited by 59 · see citing works
Risk Factors Associated With Cardiovascular Events During Testosterone Administration in Older Men With Mobility Limitation
Basaria, S. S., Davda, M. N., Travison, T. G., Ulloor, J., Singh, R., & Bhasin, S. (2012) · The Journals of Gerontology Series A · doi:10.1093/gerona/gls138 · Cited by 56 · see citing works
Relation of Visceral Adiposity to Circulating Natriuretic Peptides in Ambulatory Individuals
Cheng, S. C., Fox, C. S., Larson, M. G., Massaro, J. M., McCabe, E. L., Khan, A. M., Levy, D. A., Hoffmann, U., O’Donnell, C. J., Miller, K. K., et al. (2011) · The American Journal of Cardiology · doi:10.1016/j.amjcard.2011.05.033 · Cited by 56 · see citing works
Do low testosterone levels contribute to ill-health during male ageing?
Yeap, B. B., de Araújo, A. B., & Wittert, G. (2012) · Critical Reviews in Clinical Laboratory Sciences · doi:10.3109/10408363.2012.725461 · Cited by 53 · see citing works
Androgen Physiology, Pharmacology, and Abuse
Handelsman, D. J. (2015) · Elsevier eBooks · doi:10.1016/b978-0-323-18907-1.00138-4 · Cited by 52 · see citing works
The Salivary Microbiome in Polycystic Ovary Syndrome (PCOS) and Its Association with Disease-Related Parameters: A Pilot Study
Lindheim, L., Bashir, M., Münzker, J., Trummer, C., Zachhuber, V., Pieber, T. R., Gorkiewicz, G., & Obermayer-Pietsch, B. M. (2016) · Frontiers in Microbiology · doi:10.3389/fmicb.2016.01270 · Cited by 52 · see citing works
Metabolic Syndrome, Androgens, and Hypertension
Moulana, M., Lima, R., & Reckelhoff, J. F. (2011) · Current Hypertension Reports · doi:10.1007/s11906-011-0184-0 · Cited by 51 · see citing works
High Serum SHBG Predicts Incident Vertebral Fractures in Elderly Men
Vandenput, L., Mellström, D., Kindmark, A., Johansson, H., Lorentzon, M., Leung, J. C. S., Redlund-Johnell, I., Rosengren, B. E., Karlsson, M. K., Wáng, Y. X. J., et al. (2015) · Journal of Bone and Mineral Research · doi:10.1002/jbmr.2718 · Cited by 49 · see citing works
Testosterone imbalance may link depression and increased body weight in premenopausal women
Stanikova, D., Zsido, R. G., Luck, T., Pabst, A., Enzenbach, C., Bae, Y. J., Thiery, J., Ceglarek, U., Engel, C., Wirkner, K., et al. (2019) · Translational Psychiatry · doi:10.1038/s41398-019-0487-5 · Cited by 47 · see citing works
Preclinical challenges in steroid analysis of human samples
Ceglarek, U., Werner, M., Kortz, L., Körner, A., Kieß, W., Thiery, J., & Kratzsch, J. (2010) · The Journal of Steroid Biochemistry and Molecular Biology · doi:10.1016/j.jsbmb.2010.03.039 · Cited by 47 · see citing works
Soy Isoflavones for Reducing Bone Loss Study
Alekel, D. L., Genschel, U., Koehler, K. J., Hofmann, H., Loan, M. D. V., Beer, B. S., Hanson, L. N., Peterson, C. T., & Kurzer, M. S. (2014) · Menopause The Journal of The North American Menopause Society · doi:10.1097/gme.0000000000000280 · Cited by 47 · see citing works
Gynaecomastia in 786 adult men: clinical and biochemical findings
Mieritz, M. G., Christiansen, P., Jensen, M. B., Joensen, U. N., Nordkap, L., Olesen, I. A., Bang, A. K., Juul, A., & Jørgensen, N. (2017) · European Journal of Endocrinology · doi:10.1530/eje-16-0643 · Cited by 46 · see citing works
The heritability of circulating testosterone, oestradiol, oestrone and sex hormone binding globulin concentrations in men: the Framingham Heart Study
Travison, T. G., Zhuang, W. V., Lunetta, K. L., Karasik, D., Bhasin, S., Kiel, D. P., Coviello, A. D., & Murabito, J. M. (2014) · Clinical Endocrinology · doi:10.1111/cen.12260 · Cited by 41 · see citing works
Free testosterone and free dihydrotestosterone throughout the life span of men
Stárka, L., Pospíšilová, H., & Hill, M. (2009) · The Journal of Steroid Biochemistry and Molecular Biology · doi:10.1016/j.jsbmb.2009.05.008 · Cited by 41 · see citing works
Long-Term Testosterone Administration on Insulin Sensitivity in Older Men With Low or Low-Normal Testosterone Levels
Huang, G., Pencina, K. M., Li, Z., Basaria, S., Bhasin, S., Travison, T. G., Storer, T. W., Harman, S. M., & Tsitouras, P. (2018) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jc.2017-02545 · Cited by 40 · see citing works
Bone Density, Structure and Strength, and Their Determinants in Aging Sprint Athletes
Korhonen, M. T., Heinonen, A. O., SIEKKINEN, J., Isolehto, J., Alén, M., Kiviranta, I., & Suominen, H. (2012) · Medicine & Science in Sports & Exercise · doi:10.1249/mss.0b013e318267c954 · Cited by 41 · see citing works
Low Progesterone and Low Estradiol Levels Associate With Abdominal Aortic Aneurysms in Men
Ohlsson, C., Langenskiöld, M., Smidfelt, K., POUTANEN, M. H., Ryberg, H., Norlén, A., Nordanstig, J., Bergström, G. M. L., & Tivesten, Å. (2021) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/clinem/dgab867 · Cited by 41 · see citing works
Testosterone, Dihydrotestosterone, Sex Hormone Binding Globulin and Incident Diabetes among Older Men: the Cardiovascular Health Study
Joyce, K. E., Biggs, M. L., Djoussé, L., Ix, J. H., Kizer, J. R., Siscovick, D. S., Shores, M. M., Matsumoto, A. M., & Mukamal, K. J. (2016) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jc.2016-2623 · Cited by 40 · see citing works
Sex Hormone Binding Globulin: A Review of its Interactions With Testosterone and Age, and its Impact on Mortality in Men With Type 2 Diabetes
Ramachandran, S., Hackett, G. I., & Strange, R. C. (2019) · Sexual Medicine Reviews · doi:10.1016/j.sxmr.2019.06.006 · Cited by 40 · see citing works
Estradiol, but not testosterone, heightens cortisol-mediated negative feedback on pulsatile ACTH secretion and ACTH approximate entropy in unstressed older men and women
Sharma, A. N., Aoun, P., Wigham, J. R., Weist, S. M., & Veldhuis, J. D. (2014) · American Journal of Physiology-Regulatory, Integrative and Comparative Physiology · doi:10.1152/ajpregu.00551.2013 · Cited by 39 · see citing works
Free estradiol and sex hormone-binding globulin
Rosner, W. (2014) · Steroids · doi:10.1016/j.steroids.2014.08.005 · Cited by 38 · see citing works
Direct measurement of free estradiol in human serum by equilibrium dialysis–liquid chromatography–tandem mass spectrometry and reference intervals of free estradiol in women
Ray, J. A., Kushnir, M. M., Bunker, A. M., Rockwood, A. L., & Meikle, A. W. (2012) · Clinica Chimica Acta · doi:10.1016/j.cca.2012.02.028 · Cited by 38 · see citing works
Effects of Aerobic Exercise on Premenopausal Sex Hormone Levels: Results of the WISER Study, a Randomized Clinical Trial in Healthy, Sedentary, Eumenorrheic Women
Smith, A. J., Phipps, W. R., Arikawa, A. Y., O’Dougherty, M., Kaufman, B. C., Thomas, W. T. B., Schmitz, K. H., & Kurzer, M. S. (2011) · Cancer Epidemiology Biomarkers & Prevention · doi:10.1158/1055-9965.epi-10-1219 · Cited by 38 · see citing works
Relation between Sex Hormone Concentrations, Peripheral Arterial Disease, and Change in Ankle-Brachial Index: Findings from the Framingham Heart Study
Haring, R., Travison, T. G., Bhasin, S., Vasan, R. S., Wallaschofski, H., Davda, M. N., Coviello, A. D., & Murabito, J. M. (2011) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jc.2011-1068 · Cited by 37 · see citing works
Analysis of Hormone–Protein Binding in Solution by Ultrafast Affinity Extraction: Interactions of Testosterone with Human Serum Albumin and Sex Hormone Binding Globulin
Zheng, X., Bi, C., Brooks, M., & Hage, D. S. (2015) · Analytical Chemistry · doi:10.1021/acs.analchem.5b03007 · Cited by 36 · see citing works
The perinatal androgen to estrogen ratio and autistic-like traits in the general population: a longitudinal pregnancy cohort study
Jamnadass, E. S. L., Keelan, J. A., Lawson, L. P., Hickey, M., Maybery, M. T., & Whitehouse, A. (2015) · Journal of Neurodevelopmental Disorders · doi:10.1186/s11689-015-9114-9 · Cited by 35 · see citing works
Testosterone Attenuates Age-Related Fall in Aerobic Function in Mobility Limited Older Men With Low Testosterone
Storer, T. W., Bhasin, S., Travison, T. G., Pencina, K. M., Miciek, R., McKinnon, J., & Basaria, S. S. (2016) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jc.2015-4333 · Cited by 35 · see citing works
Circulating testosterone and SHBG concentrations are heritable in women: the Framingham Heart Study
Coviello, A. D., Zhuang, W. V., Lunetta, K. L., Bhasin, S., Ulloor, J., Zhang, A., Karasik, D., Kiel, D. P., Vasan, R. S., & Murabito, J. M. (2011) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jc.2011-0050 · Cited by 33 · see citing works
Serum Estradiol Levels Are Inversely Associated With Cortical Porosity in Older Men
Vandenput, L., Lorentzon, M., Sundh, D., Nilsson, M., Karlsson, M. K., Mellström, D., & Ohlsson, C. (2014) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jc.2014-1319 · Cited by 33 · see citing works
Circulating Estrone Levels Are Associated Prospectively With Diabetes Risk in Men of the Framingham Heart Study
Jasuja, G. K., Travison, T. G., Davda, M. N., Rose, A. J., Zhang, A., Kushnir, M. M., Rockwood, A. L., Meikle, W. A., Coviello, A. D., D’Agostino, R. B., et al. (2013) · Diabetes Care · doi:10.2337/dc12-2477 · Cited by 32 · see citing works
Green Tea Catechin Extract Supplementation Does Not Influence Circulating Sex Hormones and Insulin-Like Growth Factor Axis Proteins in a Randomized Controlled Trial of Postmenopausal Women at High Risk of Breast Cancer
Samavat, H., Wu, A. H., Ursin, G., Torkelson, C. J., Wang, R., Yu, M. C., Yee, D., Kurzer, M. S., & Yuan, J. (2019) · Journal of Nutrition · doi:10.1093/jn/nxy316 · Cited by 33 · see citing works
Dehydroepiandrosterone Sulfate and Free Testosterone but not Estradiol are Related to Muscle Strength and Bone Microarchitecture in Older Adults
Kong, S. H., Kim, J. H., Lee, J. H., Hong, A. R., Shin, C. S., & Cho, N. H. (2019) · Calcified Tissue International · doi:10.1007/s00223-019-00566-5 · Cited by 30 · see citing works
Muscles of the trunk and pelvis are responsive to testosterone administration: data from testosterone dose–response study in young healthy men
Tapper, J., Arver, S., Pencina, K. M., Martling, A., Blomqvist, L., Buchli, C., Li, Z., Gagliano‐Jucá, T., Travison, T. G., Huang, G. T.-W., et al. (2017) · Andrology · doi:10.1111/andr.12454 · Cited by 29 · see citing works
Effects of glucose ingestion on circulating inflammatory mediators: Influence of sex and weight excess
Escobar-Morreale, H. F., Martínez-García, M. Á., Montes‐Nieto, R., Fernández‐Durán, E., Temprano‐Carazo, S., & Luque‐Ramírez, M. (2016) · Clinical Nutrition · doi:10.1016/j.clnu.2016.01.015 · Cited by 29 · see citing works
Hypothalamic Gliosis by MRI and Visceral Fat Mass Negatively Correlate with Plasma Testosterone Concentrations in Healthy Men
Berkseth, K. E., Rubinow, K. B., Melhorn, S. J., Webb, M. F., Leon, M. R. B. D., Marck, B. T., Matsumoto, A. M., Amory, J. K., Page, S. T., & Schur, E. A. (2018) · Obesity · doi:10.1002/oby.22324 · Cited by 29 · see citing works
Sex hormones are negatively associated with vertebral bone marrow fat
Mistry, S., Woods, G. N., Sigurdsson, S. O., Ewing, S. K., Hue, T. F., Eiríksdóttir, G., Xu, K., Hilton, J. F., Kado, D. M., Gudnason, V. G., et al. (2017) · Bone · doi:10.1016/j.bone.2017.12.009 · Cited by 28 · see citing works
Circulating Sex Steroids and Vascular Calcification in Community-Dwelling Men: The Framingham Heart Study
Travison, T. G., O’Donnell, C. J., Bhasin, S., Massaro, J. M., Hoffmann, U., Vasan, R. S., D’Agostino, R. B., & Basaria, S. S. (2016) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jc.2015-4299 · Cited by 27 · see citing works
Effects of Testosterone Replacement on Electrocardiographic Parameters in Men: Findings from Two Randomized Trials
Gagliano‐Jucá, T., İçli, T. B., Pencina, K. M., Li, Z., Tapper, J., Huang, G. T.-W., Travison, T. G., Tsitouras, P. D., Harman, S. M., Storer, T. W., et al. (2016) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jc.2016-3669 · Cited by 26 · see citing works
Estrogen receptor 1 gene variants and estradiol activities in alcohol dependence
Mühle, C., Barry, B. K., Weinland, C., Kornhuber, J., & Lenz, B. (2019) · Progress in Neuro-Psychopharmacology and Biological Psychiatry · doi:10.1016/j.pnpbp.2019.01.008 · Cited by 27 · see citing works
Androgens, health and sexuality in women and men
Wylie, K. R., REES, M. C. P., Hackett, G. I., Anderson, R. A., Bouloux, P.-M. G., Cust, M., Goldmeier, D., Kell, P. D., TERRY, T. R., TRINICK, T. R., et al. (2010) · Human Fertility · doi:10.3109/14647273.2010.530966 · Cited by 26 · see citing works
Laboratory testing for secondary osteoporosis evaluation
Adler, R. A. (2012) · Clinical Biochemistry · doi:10.1016/j.clinbiochem.2012.01.024 · Cited by 26 · see citing works
New reference intervals for cortisol, cortisol binding globulin and free cortisol index in women using ethinyl estradiol
Panton, K. K., Mikkelsen, G., Irgens, W. Ø., Hovde, A. K., Killingmo, M. W., Øien, M. A., Thorsby, P. M., & Åsberg, A. (2019) · Scandinavian Journal of Clinical and Laboratory Investigation · doi:10.1080/00365513.2019.1622031 · Cited by 25 · see citing works
Allosterically Coupled Multisite Binding of Testosterone to Human Serum Albumin
Jayaraj, A., Schwanz, H. A., Spencer, D. J., Bhasin, S., Hamilton, J. A., Jayaram, B., Goldman, A. L., Krishna, M., Krishnan, M., Shah, A., et al. (2021) · Endocrinology · doi:10.1210/endocr/bqaa199 · Cited by 28 · see citing works
Reference intervals for free testosterone in adult men measured using a standardized equilibrium dialysis procedure
Jasuja, R., Pencina, K. M., Spencer, D. J., Peng, L., Privat, F., Dhillo, W. S., Jayasena, C. N., Hayes, F. J., Yeap, B. B., Matsumoto, A. M., et al. (2022) · Andrology · doi:10.1111/andr.13310 · Cited by 25 · see citing works
Serum androgens and risk of atrial fibrillation in older men: The Cardiovascular Health Study
Rosenberg, M. A., Shores, M. M., Matsumoto, A. M., Bůžková, P., Lange, L. A., Kronmal, R. A., Heckbert, S. R., & Mukamal, K. J. (2018) · Clinical Cardiology · doi:10.1002/clc.22965 · Cited by 25 · see citing works
Estradiol induces allosteric coupling and partitioning of sex-hormone-binding globulin monomers among conformational states
Jasuja, R., Spencer, D. J., Jayaraj, A., Peng, L., Krishna, M., Lawney, B. P., Patel, P., Jayaram, B., Thayer, K. M., Beveridge, D. L., et al. (2021) · iScience · doi:10.1016/j.isci.2021.102414 · Cited by 24 · see citing works
Evaluating Calculated Free Testosterone as a Predictor of Morbidity and Mortality Independent of Testosterone for Cross-sectional and 5-Year Longitudinal Health Outcomes in Older Men: The Concord Health and Ageing in Men Project
Hsu, B., Cumming, R. G., Blyth, F., Naganathan, V., Waite, L. M., Couteur, D. G. L., Seibel, M. J., & Handelsman, D. J. (2017) · The Journals of Gerontology Series A · doi:10.1093/gerona/glx170 · Cited by 24 · see citing works
Sex differences in serum steroid hormone levels during embryonic development in hen eggs
Wang, Y., Jin, G., Ma, M., & Xiang, X. (2019) · Poultry Science · doi:10.3382/ps/pez270 · Cited by 22 · see citing works
Elevated Levels of Estradiol in Human Immunodeficiency Virus–Infected Pregnant Women on Protease Inhibitor–Based Regimens
Balogun, K. A., Lenis, M. S. G., Papp, E., Loutfy, M. R., Yudin, M. H., MacGillivray, J., Walmsley, S. L., Silverman, M. E., & Serghides, L. (2017) · Clinical Infectious Diseases · doi:10.1093/cid/cix761 · Cited by 22 · see citing works
New Biomarkers to Evaluate Hyperandrogenemic Women and Hypogonadal Men
Karakas, S. E., & Surampudi, P. N. (2018) · Advances in clinical chemistry · doi:10.1016/bs.acc.2018.06.001 · Cited by 22 · see citing works
Effects of vitamin D on sex steroids, luteinizing hormone, and testosterone to luteinizing hormone ratio in 307 infertile men
Holt, R., Yahyavi, S. K., Kooij, I., Poulsen, N. N., Juul, A., Jørgensen, N., & Blomberg Jensen, M. (2024) · Andrology · doi:10.1111/andr.13505 · Cited by 21 · see citing works
Sex steroids and cardiovascular disease
Yeap, B. B. (2014) · Asian Journal of Andrology · doi:10.4103/1008-682x.122357 · Cited by 20 · see citing works
A Perspective on the Evolving Landscape in Male Reproductive Medicine
Bhasin, S. (2016) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jc.2015-3843 · Cited by 19 · see citing works

Calculation of free and bound fractions of testosterone and estradiol-17β to human plasma proteins at body temperature
Södergard, R., Bäckström, T., Shanbhag, V., & Carstensen, H. (1982) · Journal of Steroid Biochemistry · doi:10.1016/0022-4731(82)90038-3 · Cited by 1,080 · see citing works
An assay for sex hormone binding globulin based on affinity chromatography
Ratajczak, T., & Hähnel, R. (1976) · Clinica Chimica Acta · doi:10.1016/0009-8981(76)90138-8 · Cited by 8 · see citing works
A SIMPLE AND RAPID METHOD TO MEASURE NON-PROTEIN-BOUND FRACTIONS OF CORTISOL, TESTOSTERONE AND OESTRADIOL BY EQUILIBRIUM DIALYSIS: COMPARISON WITH CENTRIFUGAL FILTRATION
Kley, H. K., Bartmann, E., & Krüskemper, H. L. (1977) · European Journal of Endocrinology · doi:10.1530/acta.0.0850209 · Cited by 76 · see citing works
Simultaneous Determination of Free Testosterone and Testosterone Bound to Non-Sex-Hormone-Binding Globulin by Equilibrium Dialysis
Barini, A., Liberale, I., & Menini, E. (1993) · Clinical Chemistry · doi:10.1093/clinchem/39.6.936 · Cited by 5 · see citing works
Validity of free testosterone and free estradiol determinations in serum samples from postmenopausal women by theoretical calculations.
Rinaldi, S., Geay, A., Déchaud, H., Biessy, C., Zeleniuch‐Jacquotte, A., Akhmedkhanov, A., Shore, R. E., Ríboli, E., Toniolo, P. G., & Kaaks, R. (2002) · PubMed · Cited by 240 · see citing works

Authors: Mazer, N. A.
Affiliations: Boston University (US)
Corresponding author: Mazer, Norman A.
ORCID: Mazer, Norman A.
Venue: Steroids
Publisher: Elsevier BV
Published: 2009-06
Volume: 74
Issue: 6
Pages: 512-519
ISSN: 0039-128X (ISSN-L)
DOI: 10.1016/j.steroids.2009.01.008
OpenAlex: W2003710746
Cited by: 189
References: 53
Citations by year: 2026: 7 · 2025: 11 · 2024: 8 · 2023: 8 · 2022: 9 · 2021: 11 · 2020: 5 · 2019: 13 · 2018: 13 · 2017: 17 · 2016: 11 · 2015: 11 · 2014: 11 · 2013: 13 · 2012: 11
FWCI: 9.84
Type: journal article
Language: en
Open access: closed
Licence: www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations: Steroids (publishedVersion)
PubMed (publishedVersion)
Funders: National Institutes of Health (5p30ag031679-15)
National Institute on Aging (U01AG014369, R01AG031206, 1 P30 AG031679, 5UO1AG14369)
Topics: Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Hormonal and reproductive studies
Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Adrenal Hormones and Disorders
Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Estrogen and related hormone effects
Keywords: sex hormone-binding globulin, albumin, corticosteroid-binding globulin, testosterone, dihydrotestosterone, estradiol, estrone, cortisol, competitive binding, pregnancy, oophorectomy, conjugated equine estrogens, binding constant
MeSH terms: Adult, Aged, Algorithms, Estradiol, Estrone, Female, Humans, Hydrocortisone, Male, Mathematical Computing, Ovariectomy, Pregnancy, Pregnancy Trimester, Third, Reference Values, Dihydrotestosterone, Testosterone, Reproducibility of Results

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

A novel spreadsheet method for calculating the free serum concentrations of testosterone, dihydrotestosterone, estradiol, estrone and cortisol: With illustrative examples from male and female populations

Abstract

Integrity signals

Claims the paper makes

Claims other papers make about this one

Cites

Cited by

Related

Record

About these checks