IFT–Cargo Interactions and Protein Transport in Cilia

Lechtreck, Karl F.

doi:10.1016/j.tibs.2015.09.003

Cited by 316Genetic and Kidney Cyst DiseasesCystic Fibrosis Research AdvancesHereditary Neurological Disorders

IFT–Cargo Interactions and Protein Transport in Cilia

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Lechtreck, Karl F.·2015·Trends in Biochemical Sciences

DOI 10.1016/j.tibs.2015.09.003PMC PMC4661101

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Abstract

The motile and sensory functions of cilia and flagella are indispensable for human health. Cilia assembly requires a dedicated protein shuttle, intraflagellar transport (IFT), a bidirectional motility of multi-megadalton protein arrays along ciliary microtubules. IFT functions as a protein carrier delivering hundreds of distinct proteins into growing cilia. IFT-based protein import and export continue in fully grown cilia and are required for ciliary maintenance and sensing. Large ciliary building blocks might depend on IFT to move through the transition zone, which functions as a ciliary gate. Smaller, freely diffusing proteins, such as tubulin, depend on IFT to be concentrated or removed from cilia. As I discuss here, recent work provides insights into how IFT interacts with its cargoes and how the transport is regulated.

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Progress has been made in understanding interactions between specific IFT particle polypeptides and specific ciliary cargo proteins or motor subunits during anterograde or retrograde IFT, as well as how IFT motor activity is regulated.
“Likewise, progress has been made towards understanding the interactions between specific IFT particle polypeptides and specific ciliary cargo proteins or motor subunits during anterograde or retrograde IFT, as well as how IFT motor activity is regulated 24, 48–52.”
From Cellular signalling by primary cilia in development, organ function and disease (2019) · cites this paper as [24]
The sections on cargo binding and known IFT binding sites describe specific interactions, including IFT81/IFT74 with tubulin and IFT46 with an adaptor for outer dynein arms. The building-blocks section identifies the motors for anterograde and retrograde transport, but the paper does not substantively explain how IFT motor activity is regulated or establish the claimed interactions with specific motor subunits. Page numbers were unavailable in our copy. (LLM, conf=0.82)
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The intraflagellar transport system moves along axonemal microtubules to carry specific ciliary cargo proteins into or out of the organelle.
“and the intraflagellar transport (IFT) system that zips up and down axonemal microtubules to mediate the transport of specific ciliary cargo proteins into or out of the organelle 24, 25 (Figure 1).”
From Cellular signalling by primary cilia in development, organ function and disease (2019) · cites this paper as [24]

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Authors: Lechtreck, K. F.
Affiliations: University of Georgia (US)
Corresponding author: Lechtreck, Karl F.
ORCID: Lechtreck, Karl F.
Venue: Trends in Biochemical Sciences
Publisher: Elsevier BV
Published: 2015-12
Volume: 40
Issue: 12
Pages: 765-778
ISSN: 0968-0004 (ISSN-L)
DOI: 10.1016/j.tibs.2015.09.003
PMC: PMC4661101
OpenAlex: W1809267070
Cited by: 316
References: 104
Citations by year: 2026: 8 · 2025: 17 · 2024: 24 · 2023: 35 · 2022: 28 · 2021: 37 · 2020: 42 · 2019: 32 · 2018: 32 · 2017: 35 · 2016: 24 · 2015: 1 · 2014: 1
FWCI: 3.94
Type: journal article
Language: en
Open access: green
www.ncbi.nlm.nih.gov
Licence: www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations: Trends in Biochemical Sciences (publishedVersion)
PubMed (publishedVersion)
PubMed Central (acceptedVersion)
Funders: National Institute of General Medical Sciences (R01GM110413, GM110413)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic and Kidney Cyst Diseases
Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Cystic Fibrosis Research Advances
Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Hereditary Neurological Disorders
Keywords: intraflagellar transport, cilia, flagella, transition zone, ciliary microtubules, ciliary assembly, transport regulation
MeSH terms: Animals, Cilia, Humans, Microtubules, Proteins, Protein Transport

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IFT–Cargo Interactions and Protein Transport in Cilia

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