Glucose metabolism in mammalian cell culture: new insights for tweaking vintage pathways
Abstract
Cultured mammalian cells are major vehicles for producing therapeutic proteins, and energy metabolism in those cells profoundly affects process productivity. The characteristic high glucose consumption and lactate production of industrial cell lines as well as their adverse effects on productivity have been the target of both cell line and process improvement for several decades. Recent research advances have shed new light on regulation of glucose metabolism and its links to cell proliferation. This review highlights our current understanding in this area of crucial importance in bioprocessing and further discusses strategies for harnessing new findings toward process enhancement through the manipulation of cellular energy metabolism.
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Glycolysis is one of the most ancient metabolic pathways with a wide variety of allosteric and phosphorylation-based controls.
“Glycolysis is one of the most ancient metabolic pathways with a wide variety of allosteric and phosphorylation-based controls (Mulukutla et al., 2010; Icard and Lincet, 2012).”
From Fueling thought: Management of glycolysis and oxidative phosphorylation in neuronal metabolism (2018) · cites this paper as Mulukutla et al., 2010
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- Authors
- Mulukutla, B. C., Khan, S., Lange, A., & Hu, W.-S.
- Affiliations
- University of Minnesota (US)
- Corresponding author
- Hu, Wei-Shou
- Venue
- Trends in biotechnology
- Publisher
- Elsevier BV
- Published
- 2010-09
- Volume
- 28
- Issue
- 9
- Pages
- 476-484
- ISSN
- 0167-7799 (ISSN-L)
- OpenAlex
- W2005835980
- Cited by
- 132
- References
- 61
- Citations by year
- 2026: 3 · 2025: 4 · 2024: 7 · 2023: 7 · 2022: 8 · 2021: 10 · 2020: 14 · 2019: 11 · 2018: 11 · 2017: 6 · 2016: 7 · 2015: 9 · 2014: 5 · 2013: 16 · 2012: 8
- FWCI
- 0.81
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Trends in biotechnology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Viral Infectious Diseases and Gene Expression in Insects
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer, Hypoxia, and Metabolism
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Keywords
- glucose metabolism, mammalian cell culture, energy metabolism, therapeutic protein production, glucose utilization, lactic acid production, cell proliferation, process improvement, bioprocessing
- MeSH terms
- Animals, Glucose, Humans, Signal Transduction, Cell Culture Techniques, Systems Biology, Metabolic Networks and Pathways
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