Cited by 132Viral Infectious Diseases and Gene Expression in InsectsCancer, Hypoxia, and MetabolismMicrobial Metabolic Engineering and Bioproduction

Glucose metabolism in mammalian cell culture: new insights for tweaking vintage pathways

Mulukutla, Bhanu Chandra, Khan, Salmaan, Lange, Alex, Hu, Wei-Shou·2010·Trends in biotechnology

Abstract

Cultured mammalian cells are major vehicles for producing therapeutic proteins, and energy metabolism in those cells profoundly affects process productivity. The characteristic high glucose consumption and lactate production of industrial cell lines as well as their adverse effects on productivity have been the target of both cell line and process improvement for several decades. Recent research advances have shed new light on regulation of glucose metabolism and its links to cell proliferation. This review highlights our current understanding in this area of crucial importance in bioprocessing and further discusses strategies for harnessing new findings toward process enhancement through the manipulation of cellular energy metabolism.

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Crossref · 10.1016/j.tibtech.2010.06.005

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Authors
Mulukutla, B. C., Khan, S., Lange, A., & Hu, W.-S.
Affiliations
University of Minnesota (US)
Corresponding author
Hu, Wei-Shou
Venue
Trends in biotechnology
Publisher
Elsevier BV
Published
2010-09
Volume
28
Issue
9
Pages
476-484
ISSN
0167-7799 (ISSN-L)
OpenAlex
W2005835980
Cited by
132
References
61
Citations by year
2026: 3 · 2025: 4 · 2024: 7 · 2023: 7 · 2022: 8 · 2021: 10 · 2020: 14 · 2019: 11 · 2018: 11 · 2017: 6 · 2016: 7 · 2015: 9 · 2014: 5 · 2013: 16 · 2012: 8
FWCI
0.81
Type
journal article
Language
en
Open access
closed
Locations
  • Trends in biotechnology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Viral Infectious Diseases and Gene Expression in Insects
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer, Hypoxia, and Metabolism
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
Keywords
glucose metabolism, mammalian cell culture, energy metabolism, therapeutic protein production, glucose utilization, lactic acid production, cell proliferation, process improvement, bioprocessing
MeSH terms
Animals, Glucose, Humans, Signal Transduction, Cell Culture Techniques, Systems Biology, Metabolic Networks and Pathways

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