Cited by 899CRISPR and Genetic EngineeringAnimal Genetics and ReproductionRNA regulation and disease

Heritable genome editing in C. elegans via a CRISPR-Cas9 system

Open access
Friedland, Ari E, Tzur, Yonatan B, Esvelt, Kevin M, Colaiácovo, Monica P, Church, George M, Calarco, John A·2013·Nature Methods

Abstract

We report the use of clustered, regularly interspaced, short palindromic repeats (CRISPR)-associated endonuclease Cas9 to target genomic sequences in the Caenorhabditis elegans germ line using single-guide RNAs that are expressed from a U6 small nuclear RNA promoter. Our results demonstrate that targeted, heritable genetic alterations can be achieved in C. elegans, providing a convenient and effective approach for generating loss-of-function mutants.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1038/nmeth.2532

Cites

25 of 25 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Authors
Friedland, A. E., Tzur, Y. B., Esvelt, K. M., Colaiácovo, M. P., Church, G. M., & Calarco, J. A.
Affiliations
Harvard University (US)
Corresponding author
  • Church, George M
  • Calarco, John A
Venue
Nature Methods
Published
2013-06-30
Volume
10
Issue
8
Pages
741-743
ISSN
  • 1548-7091 (ISSN-L)
  • 1548-7105
OpenAlex
W2133702185
Cited by
899
References
25
Citations by year
2026: 19 · 2025: 24 · 2024: 29 · 2023: 36 · 2022: 54 · 2021: 69 · 2020: 66 · 2019: 57 · 2018: 108 · 2017: 84 · 2016: 109 · 2015: 91 · 2014: 111 · 2013: 41 · 2012: 1
FWCI
52.51
Type
journal article
Language
en
Open access
Locations
  • Nature Methods (publishedVersion)
  • PubMed (publishedVersion)
  • Europe PMC (PubMed Central) (acceptedVersion)
  • PubMed Central (acceptedVersion)
  • Digital Access to Scholarship at Harvard (DASH) (Harvard University) (submittedVersion, cc-by)
Funders
  • National Institutes of Health (DP5OD009153)
  • National Human Genome Research Institute (P50HG005550)
  • National Institute of General Medical Sciences (R01GM072551)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Animal Genetics and Reproduction
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA regulation and disease
Keywords
CRISPR/Cas9, genome editing, Caenorhabditis elegans, germline, heritable genetic modification, single guide RNA, RNA polymerase III, loss-of-function mutants, targeted mutagenesis
MeSH terms
Animals, Genetic Engineering, Promoter Regions, Genetic, RNA, Small Nuclear, Genome, Caenorhabditis elegans, Zebrafish Proteins, Inverted Repeat Sequences, RNA, Small Untranslated

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review