Heritable genome editing in C. elegans via a CRISPR-Cas9 system
Open accessAbstract
We report the use of clustered, regularly interspaced, short palindromic repeats (CRISPR)-associated endonuclease Cas9 to target genomic sequences in the Caenorhabditis elegans germ line using single-guide RNAs that are expressed from a U6 small nuclear RNA promoter. Our results demonstrate that targeted, heritable genetic alterations can be achieved in C. elegans, providing a convenient and effective approach for generating loss-of-function mutants.
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The CRISPR/Cas system has been successfully applied to human cells and several model organisms for genome editing.
“Recently, the CRISPR/Cas system has been successfully applied to human cells19,20,21,22 and several model organisms for genome editing.23,24,25,26”
From The CRISPR/Cas9 system facilitates clearance of the intrahepatic HBV templates in vivo (2014) · cites this paper as [23]
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Recent Progress in CRISPR/Cas9 Technology
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How CRISPR-Cas System Could Be Used to Combat Antimicrobial Resistance
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Genome engineering using the CRISPR/Cas system
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SapTrap, a Toolkit for High-Throughput CRISPR/Cas9 Gene Modification in Caenorhabditis elegans
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RNA Interference in Caenorhabditis elegans
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Precise Gene Deletion and Replacement Using the CRISPR/Cas9 System in Human Cells
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Optimization of CRISPR/Cas9 genome editing in cotton by improved sgRNA expression
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- Authors
- Friedland, A. E., Tzur, Y. B., Esvelt, K. M., Colaiácovo, M. P., Church, G. M., & Calarco, J. A.
- Affiliations
- Harvard University (US)
- Corresponding author
- Church, George M
- Calarco, John A
- Venue
- Nature Methods
- Published
- 2013-06-30
- Volume
- 10
- Issue
- 8
- Pages
- 741-743
- ISSN
- 1548-7091 (ISSN-L)
- 1548-7105
- PMC
- PMC3822328
- OpenAlex
- W2133702185
- Cited by
- 899
- References
- 25
- Citations by year
- 2026: 19 · 2025: 24 · 2024: 29 · 2023: 36 · 2022: 54 · 2021: 69 · 2020: 66 · 2019: 57 · 2018: 108 · 2017: 84 · 2016: 109 · 2015: 91 · 2014: 111 · 2013: 41 · 2012: 1
- FWCI
- 52.51
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature Methods (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Digital Access to Scholarship at Harvard (DASH) (Harvard University) (submittedVersion, cc-by)
- Funders
- National Institutes of Health (DP5OD009153)
- National Human Genome Research Institute (P50HG005550)
- National Institute of General Medical Sciences (R01GM072551)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Animal Genetics and Reproduction
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA regulation and disease
- Keywords
- CRISPR/Cas9, genome editing, Caenorhabditis elegans, germline, heritable genetic modification, single guide RNA, RNA polymerase III, loss-of-function mutants, targeted mutagenesis
- MeSH terms
- Animals, Genetic Engineering, Promoter Regions, Genetic, RNA, Small Nuclear, Genome, Caenorhabditis elegans, Zebrafish Proteins, Inverted Repeat Sequences, RNA, Small Untranslated
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