Immunotherapeutic effects of mangiferin mediated by the inhibition of oxidative stress to activated lymphocytes, neutrophils and macrophages
Abstract
The effect of mangiferin, a naturally occurring xanthone glucoside on cyclophosphamide-induced immunotoxicity and its mode of action in the immune system were investigated. To induce immunotoxicity, adult male Wistar rats were injected weekly with cyclophosphamide intraperitoneally at 100 mg/kg bodyweight. Mangiferin was injected intraperitoneally at 10 and 20 mg/kg daily for 14 days. Levamisole (3 mg/kg, i.p., daily for 14 days), a known immunostimulant that acts in immunosuppressive conditions was used as a standard drug. The effect of mangiferin on the primary immune response to ovalbumin (200 microg/rat, s.c.) was assessed at weekly intervals by measuring the serum ovalbumin-specific IgM levels. The organ weights and cellularity of spleen, thymus and bone marrow, haematology, T and B cell-dependent mitogen stimulation of splenocytes were assessed for the cellular response. Oxidative changes in lymphocytes, neutrophils and macrophages were measured at the end of the study. As well, the in vitro effect of mangiferin on cytotoxicity caused by H2O2 in primary lymphocytes was studied. The decrease in the lymphoid organ weights, cellular responses and antigen-specific IgM levels by cyclophosphamide treatment were significantly increased by repeated intraperitoneal administration of mangiferin. The enhanced lipid peroxidation and decreased catalase and superoxide dismutase activities found in lymphocytes, polymorphonuclear cells (PMN) and macrophages from cyclophosphamide treated rats were significantly ameliorated in mangiferin treated groups. The tissue injury caused by cyclophosphamide treatment was significantly suppressed by mangiferin as shown by the decrease in serum creatine phosphokinase (CPK) activity. In vitro experiments showed that pretreatment of lymphocytes with mangiferin protected from the toxicity induced by H2O2, further confirming the in vivo findings. From this study, it is evident that mangiferin exhibits an immunoprotective role mediated through the inhibition of reactive intermediate-induced oxidative stress in lymphocytes, neutrophils and macrophages.
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Mangiferin exerts an immunoprotective role via inhibition of reactive intermediate-induced oxidative stress in lymphocytes, neutrophils and macrophages.
“Thus, it was evident that mangiferin exerts an immunoprotective role via inhibition of reactive intermediate-induced oxidative stress in lymphocytes, neutrophils and macrophages (108).”
From Mangiferin: An effective therapeutic agent against several disorders (Review) (2018) · cites this paper as [108]
Mangiferin markedly suppresses tissue injury and immunotoxicity caused by cyclophosphamide treatment, through decreasing serum CPK activity and antigen-specific IgM levels.
“In addition, mangiferin markedly suppresses tissue injury and immunotoxicity caused by cyclophosphamide treatment, through decreasing serum CPK activity and antigen-specific IgM levels (108).”
From Mangiferin: An effective therapeutic agent against several disorders (Review) (2018) · cites this paper as [108]
Cites
PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT
Lowry, O. H., Rosebrough, N., Farr, A. L., & Randall, R. (1951) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)52451-6 · Cited by 315,771 · see citing works
Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction
Ohkawa, H., Ohishi, N., & Yagi, K. (1979) · Analytical Biochemistry · doi:10.1016/0003-2697(79)90738-3 · Cited by 29,460 · see citing works
Methods of Biochemical Analysis
(1956) · The Medical Journal of Australia · doi:10.5694/j.1326-5377.1956.tb56924.x · Cited by 4,489 · see citing works
Microtiter plate assay for superoxide dismutase using MTT reduction by superoxide.
Madesh, M., & Balasubramanian, K. A. (1998) · PubMed · Cited by 524 · see citing works
Effect of mangiferin on hyperglycemia and atherogenicity in streptozotocin diabetic rats
Muruganandan, S., Srinivasan, K., Gupta, S., Gupta, P. K., & Lal, J. (2005) · Journal of Ethnopharmacology · doi:10.1016/j.jep.2004.12.010 · Cited by 426 · see citing works
Self-citationCardiotoxicity of cytotoxic drugs
Schimmel, K. J. M., Richel, D. J., van den Brink, R. B. A., & Guchelaar, H. (2003) · Cancer Treatment Reviews · doi:10.1016/j.ctrv.2003.07.003 · Cited by 391 · see citing works
Martı́nez-Sánchez, G., Re, L., Giuliani, A., Sellés, A. J. N., Pérez-Davison, G., & León-Fernández, O. S. (2000) · Pharmacological Research · doi:10.1006/phrs.2000.0727 · Cited by 302 · see citing works
Guha, S., Ghosal, S., & Chattopadhyay, U. (2009) · Chemotherapy · doi:10.1159/000239478 · Cited by 275 · see citing works
Immunomodulatory activity of alcoholic extract of Mangifera indica L. in mice
Makare, N., Bodhankar, S., & Rangari, V. (2001) · Journal of Ethnopharmacology · doi:10.1016/s0378-8741(01)00326-9 · Cited by 258 · see citing works
Mangiferin protects the streptozotocin-induced oxidative damage to cardiac and renal tissues in rats
Muruganandan, S., Gupta, S., Kataria, M., Lal, J., & Gupta, P. K. (2002) · Toxicology · doi:10.1016/s0300-483x(02)00069-0 · Cited by 216 · see citing works
Self-citationAntidiabetic activity of a xanthone compound, mangiferin
Miura, T., Ichiki, H., Hashimoto, I., Iwamoto, N., Kao, M., Kubo, M., Ishihara, E., Komatsu, Y., Okada, M., Ishida, T., et al. (2001) · Phytomedicine · doi:10.1078/0944-7113-00009 · Cited by 206 · see citing works
Anti-herpes simplex virus activities of crude water extracts of Thai Medicinal Plants
Yoosook, C., Bunyapraphatsara, N., Boonyakiat, Y., & Kantasuk, C. (2000) · Phytomedicine · doi:10.1016/s0944-7113(00)80068-9 · Cited by 175 · see citing works
New Antidiabetic Compounds, Mangiferin and Its Glucoside.
Ichiki, H., Miura, T., Kubo, M., Ishihara, E., KOMATSU, Y., Tanigawa, K., & Okada, M. (1998) · Biological and Pharmaceutical Bulletin · doi:10.1248/bpb.21.1389 · Cited by 158 · see citing works
Leiro, J. M., Arranz, J. A., Yáñez, M., Ubeira, F. M., Sanmartín, M. L., & Orallo, F. (2004) · International Immunopharmacology · doi:10.1016/j.intimp.2004.03.002 · Cited by 152 · see citing works
Bounous, D. I., Campagnoli, R. P., & Brown, J. (1992) · Avian Diseases · doi:10.2307/1591566 · Cited by 151 · see citing works
Ballarin, L., Dall'Oro, M., Bertotto, D., et al. (2004) · Comparative Biochemistry and Physiology Part A Molecular & Integrative Physiology · doi:10.1016/j.cbpb.2004.02.019 · Cited by 138 · see citing works
Andreu, G. P., Delgado, R., Velho, J. A., Curti, C., & Vercesi, A. E. (2005) · European Journal of Pharmacology · doi:10.1016/j.ejphar.2005.03.007 · Cited by 122 · see citing works
Antiviral effect of mangiferin and isomangiferin on herpes simplex virus.
Zheng, M. S., & Lu, Z. (1990) · PubMed · Cited by 111 · see citing works
Garrido, G. (2004) · Pharmacological Research · doi:10.1016/j.phrs.2003.12.020 · Cited by 104 · see citing works
Oxidative DNA damage induced by a hydroperoxide derivative of cyclophosphamide
Murata, M., Suzuki, T., Midorikawa, K., Oikawa, S., & Kawanishi, S. (2004) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2004.05.009 · Cited by 84 · see citing works
Radioprotection by mangiferin in DBAxC57BL mice: a preliminary study
Jagetia, G. C., & Baliga, M. S. (2005) · Phytomedicine · doi:10.1016/j.phymed.2003.08.003 · Cited by 83 · see citing works
(1989) · PubMed · Cited by 79 · see citing works
Protective effect of dl-α-lipoic acid in cyclophosphamide induced oxidative injury in rat testis
Elangovan, S., Prahalathan, C., Mythili, Y., & Varalakshmi, P. (2004) · Reproductive Toxicology · doi:10.1016/j.reprotox.2004.06.015 · Cited by 79 · see citing works
(1993) · PubMed · Cited by 76 · see citing works
Kaufmann, S. H. E., Hahn, H., & Diamantstein, T. (1980) · The Journal of Immunology · doi:10.4049/jimmunol.125.3.1104 · Cited by 60 · see citing works
Krakowski, L., Krzyżanowski, J., Wrona, Z., et al. (1999) · Veterinary Immunology and Immunopathology · doi:10.1016/s0165-2427(99)00006-9 · Cited by 57 · see citing works
Mechanisms of adjuvancy: I?metal oxides as adjuvants
Naim, J. O. (1997) · Vaccine · doi:10.1016/s0264-410x(97)00016-9 · Cited by 54 · see citing works
Geodakyan, S. V., Воскобойникова, И. В., Tjukavkina, N. A., Kolhir, V. K., Kolesnik, Y. A., Zjuzin, V. A., Glyzin, V. I., & Sokolov, S. J. (1992) · Phytotherapy Research · doi:10.1002/ptr.2650060611 · Cited by 26 · see citing works
Jagetia, G. C., & Venkatesha, V. A. (2005) · International Congress Series · doi:10.1016/j.ics.2004.11.035 · Cited by 13 · see citing works
Separation of murine peritoneal macrophages using percoll density gradients
Vray, B., & Plasman, N. (1994) · Journal of Immunological Methods · doi:10.1016/0022-1759(94)90009-4 · Cited by 12 · see citing works
Kamanyi, A., Bopelet, M., Lontsi, D., et al. (1996) · Phytomedicine · doi:10.1016/s0944-7113(96)80044-4 · Cited by 12 · see citing works
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- Maehly, 1954, vol. 1, 357
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Mangiferin: A natural miracle bioactive compound against lifestyle related disorders
Imran, M., Arshad, M. S., Butt, M. S., Kwon, J.-H., Arshad, M. U., & Sultan, M. T. (2017) · Lipids in Health and Disease · doi:10.1186/s12944-017-0449-y · Cited by 366 · see citing works
Mangiferin: An effective therapeutic agent against several disorders (Review)
Du, S., Liu, H., Lei, T., Xie, X., Wang, H., He, X., Tong, R., & Wang, Y. (2018) · Molecular Medicine Reports · doi:10.3892/mmr.2018.9529 · Cited by 139 · see citing works
“In addition, mangiferin markedly suppresses tissue injury and immunotoxicity caused by cyclophosphamide treatment, through decreasing serum CPK activity and antigen‑specific IgM levels (108).”
Bhatia, H. S., Candelario-Jalil, E., de Oliveira, A. C. P., Olajide, O. A., Martínez-Sánchez, G., & Fiebich, B. L. (2008) · Archives of Biochemistry and Biophysics · doi:10.1016/j.abb.2008.06.017 · Cited by 95 · see citing works
- Authors
- Muruganandan, S., Lal, J., & Gupta, P. K.
- Affiliations
- Indian Veterinary Research Institute (IN)
- Corresponding author
- Muruganandan, S.
- Venue
- Toxicology
- Publisher
- Elsevier BV
- Published
- 2005-11
- Volume
- 215
- Issue
- 1-2
- Pages
- 57-68
- ISSN
- 0300-483X (ISSN-L)
- OpenAlex
- W2006119196
- Semantic Scholar
- 9885db4db2a622ac5b2d45e233c47b9235fad819
- Cited by
- 77
- References
- 31
- Citations by year
- 2026: 3 · 2025: 6 · 2024: 8 · 2023: 3 · 2022: 3 · 2021: 3 · 2020: 8 · 2019: 2 · 2018: 2 · 2017: 5 · 2015: 4 · 2014: 2 · 2013: 2 · 2012: 6
- FWCI
- 6.63
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Toxicology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Complementary and alternative medicine › Mangiferin and Mango Extracts
- Life Sciences › Immunology and Microbiology › Immunology › Immunotoxicology and immune responses
- Health Sciences › Medicine › Complementary and alternative medicine › Medicinal plant effects and applications
- Keywords
- mangiferin, immunoprotection, oxidative stress, lipid peroxidation, catalase, superoxide dismutase, lymphocytes, neutrophils, macrophages, hydrogen peroxide cytotoxicity, primary immune response, levamisole, creatine kinase, tissue damage
- MeSH terms
- Adjuvants, Immunologic, Animals, Antibody Formation, Body Weight, Cell Count, Cyclophosphamide, Hydrogen Peroxide, Lymphocytes, Lymphoid Tissue, Male, Neutrophils, Organ Size, Ovalbumin, Rats, Wistar, Macrophages, Peritoneal, Oxidative Stress, Xanthones, Rats
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