Cited by 95Mangiferin and Mango ExtractsInflammatory mediators and NSAID effectsNeuroinflammation and Neurodegeneration Mechanisms

Mangiferin inhibits cyclooxygenase-2 expression and prostaglandin E2 production in activated rat microglial cells

Bhatia, Harsharan S., Candelario-Jalil, Eduardo, de Oliveira, Antonio C. Pinheiro, Olajide, Olumayokun A., Martínez-Sánchez, Gregorio, Fiebich, Bernd L.·2008·Archives of Biochemistry and Biophysics

Abstract

Mangiferin, a naturally occurring glucosylxanthone, has potent antioxidant and anti-inflammatory properties, as demonstrated in several reports. However, very limited information is available on the effects of this natural polyphenol on microglial activation. Thus, the aim of this study was to examine whether mangiferin is able to reduce prostaglandin E(2) (PGE(2)) and 8-iso-prostaglandin F(2alpha) (8-iso-PGF(2alpha)) production by lipopolysaccharide (LPS)-activated primary rat microglia. Microglial cells were stimulated with 10ng/ml of LPS in the presence or absence of different concentrations of mangiferin (1-50 microM). After 24h incubation, culture media were collected to measure the production of PGE(2) and 8-iso-PGF(2alpha) using enzyme immunoassays. Protein levels of cyclooxygenase (COX)-1 and COX-2 were studied by immunoblotting after 24h of incubation with LPS. Mangiferin potently reduced LPS-induced PGE(2) synthesis and the formation of 8-iso-PGF(2alpha). Interestingly, mangiferin dose-dependently reduced LPS-induced COX-2 protein synthesis without modifying COX-2 transcription. This was due to a decrease in COX-2 transcript stability. However, mangiferin did not modify LPS-mediated phosphorylation of p38 mitogen-activated protein kinase (p38 MAPK), a key factor involved in enhancing COX-2 mRNA stability and COX-2 translation in primary microglia. Mangiferin had no effects on LPS-induced expression of inducible nitric oxide synthase (iNOS) or TNF-alpha production. T…

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Crossref · 10.1016/j.abb.2008.06.017

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  • By decreasing COX-2 transcript stability, mangiferin potently reduces LPS-induced PGE-2 synthesis and 8-iso-PGF2alpha production.

    “By decreasing COX-2 transcript stability, mangiferin potently reduces LPS-induced prostaglandin E2 (PGE-2) synthesis and 8-iso-prostaglandin F2α (8-iso-PGF2α) production (72).”

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Authors
Bhatia, H. S., Candelario-Jalil, E., de Oliveira, A. C. P., Olajide, O. A., Martínez-Sánchez, G., & Fiebich, B. L.
Affiliations
University of Freiburg (DE)
Corresponding author
Fiebich, Bernd L.
Venue
Archives of Biochemistry and Biophysics
Publisher
Elsevier BV
Published
2008-09
Volume
477
Issue
2
Pages
253-258
ISSN
0003-9861 (ISSN-L)
OpenAlex
W2042159604
Cited by
95
References
52
Citations by year
2026: 1 · 2025: 1 · 2024: 2 · 2023: 9 · 2022: 3 · 2021: 7 · 2020: 4 · 2019: 8 · 2018: 3 · 2017: 4 · 2016: 8 · 2015: 6 · 2014: 11 · 2013: 8 · 2012: 5
FWCI
12.69
Type
journal article
Language
en
Open access
closed
Locations
  • Archives of Biochemistry and Biophysics (publishedVersion)
  • PubMed (publishedVersion)
  • University of Huddersfield Repository (University of Huddersfield) (submittedVersion)
Funders
  • American Heart Association
  • Alexander von Humboldt-Stiftung
  • Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
Topics
  • Health Sciences › Medicine › Complementary and alternative medicine › Mangiferin and Mango Extracts
  • Health Sciences › Medicine › Pharmacology › Inflammatory mediators and NSAID effects
  • Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
Keywords
mangiferin, microglial activation, cyclooxygenase-2, prostaglandin E2, 8-isoprostane, lipopolysaccharide, p38 MAPK, neuroinflammation, cerebral protection, inducible nitric oxide synthase, TNF-α
MeSH terms
Animals, Animals, Newborn, Brain, Cells, Cultured, Dose-Response Relationship, Drug, Lipopolysaccharides, Dinoprostone, Signal Transduction, Rats, Sprague-Dawley, Microglia, Oxidative Stress, Xanthones, Rats, Cyclooxygenase 2

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