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Cited by 165Connexins and lens biologyPancreatic function and diabetesIon channel regulation and function

The DOI matches its Crossref record; 62 of the 62 references it cites were found in a registry or an index.

Biophysical properties of gap junctions between freshly dispersed pairs of mouse pancreatic beta cells

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Pérez-Armendariz, M., Roy, C., Spray, D.C., Bennett, M.V.·1991·Biophysical Journal
DOI 10.1016/s0006-3495(91)82200-7PMC PMC1281120
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Abstract

Coupling between beta cells through gap junctions has been postulated as a principal mechanism of electrical synchronization of glucose-induced activity throughout the islet of Langerhans. We characterized junctional conductance between isolated pairs of mouse pancreatic beta cells by whole-cell recording with two independent patch-clamp circuits. Most pairs were coupled (67%, n = 155), although the mean junctional conductance (gj) (215 +/- 110 pS) was lower than reported in other tissues. Coupling could be recorded for long periods, up to 40 min. Voltage imposed across the junctional or nonjunctional membranes had no effect on gj. Up to several hours of treatment to increase intracellular cAMP levels did not affect gj. Electrically coupled pairs did not show transfer of the dye Lucifer yellow. Octanol (2 mM) reversibly decreased gj. Lower concentrations of octanol (0.5 mM) and heptanol (0.5 mM) than required to uncouple beta cells decreased voltage-dependent K+ and Ca2+ currents in nonjunctional membranes. Although gj recorded in these experiments would be expected to be provided by current flowing through only a few channels of the unitary conductance previously reported for other gap junctions, no unitary junctional currents were observed even during reversible suppression of gj by octanol. This result suggests either that the single channel conductance of gap junction channels between beta cells is smaller than in other tissues (less than 20 pS) or that the small mean conductance is due to transitions between open and closed states that are too rapid or too slow to be resolved.

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Metadata

Authors
Pérez-Armendariz, M., Roy, C., Spray, D. C., & Bennett, M. V.
Affiliations
Albert Einstein College of Medicine (US)
ORCID
Venue
Biophysical Journal
Publisher
Elsevier BV
Published
1991-01
Volume
59
Issue
1
Pages
76-92
ISSN
0006-3495 (ISSN-L)
DOI
10.1016/s0006-3495(91)82200-7
PMC
PMC1281120
OpenAlex
W2009143060
Cited by
165
References
62
Citations by year
2026: 2 · 2025: 1 · 2024: 2 · 2023: 3 · 2022: 3 · 2021: 3 · 2020: 2 · 2019: 6 · 2018: 3 · 2017: 8 · 2016: 3 · 2015: 4 · 2014: 10 · 2013: 8 · 2012: 5
FWCI
2.91
Type
journal article
Language
en
Open access
Licence
Locations
  • Biophysical Journal (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health (5r01ns016524-13, 5r29dk038529-05, 5p01ns007512-31)
  • National Institute of Diabetes and Digestive and Kidney Diseases (DK-38529)
  • National Institute of Neurological Disorders and Stroke (NS16524, NS-07512)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Connexins and lens biology
  • Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Pancreatic function and diabetes
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion channel regulation and function
Keywords
gap junctions, pancreatic beta cells, electrical coupling, junctional conductance, heptanol, cyclic AMP, voltage-dependent potassium current, voltage-dependent calcium current, single-channel conductance, mice
MeSH terms
Animals, Cell Membrane, Cells, Cultured, Electric Conductivity, Electrophysiology, Fluorescent Dyes, Freeze Fracturing, Intercellular Junctions, Islets of Langerhans, Isoquinolines, Membrane Potentials, Mice, Inbred Strains, Microscopy, Electron, Mice

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