Assembly and aggregation properties of synthetic Alzheimer's A4/beta amyloid peptide analogs.
Open accessAbstract
The amyloid A4 or beta peptide is a major component of extracellular amyloid deposits that are a characteristic feature of Alzheimer's disease. We synthesized a series of peptide analogs of the A4/beta peptide which are progressively longer at their carboxyl termini, including 42- and 39-residue peptides which represent the major forms of the A4/beta peptide in senile plaque and the hereditary cerebral hemorrhage with amyloidosis form, respectively. All peptides tested, beta 1-28 through beta 1-42, formed amyloid-like fibrils and previously unreported thin sheet-like structures which stained with thioflavin T and Congo Red. The solubility of beta 1-42 and shorter peptides was pH and concentration dependent, with a broad insolubility profile in the pH range of 3.5-6.5 and at concentrations above 0.75 mg/ml. Only peptides of 42 residues or longer were significantly insoluble at pH 7.4. beta 1-47 and beta 1-52 peptides are highly insoluble in aqueous media but are soluble at 40 mg/ml in the alpha helix-promoting solvent, 1,1,1,3,3,3-hexafluoro-2-propanol. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed that the beta 1-42 peptide migrates as a series of higher molecular mass aggregates whereas shorter peptides migrate as monomers. Aggregation is also dependent on pH, peptide concentration, and time of incubation in aqueous medium. These results indicate that the length of the hydrophobic carboxyl terminus of the A4/beta peptide is important in determining the s…
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Aβ1-42 forms insoluble amyloid fibrils more rapidly than Aβ1-40.
“A{31-42 has been shown to form insoluble amyloid fibrils more rapidly than A{31-40 (10)(11)(12)(13).”
From Amyloid β Protein (Aβ) in Alzheimeri's Disease Brain (1995) · cites this paper as [11]
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- Authors
- Burdick, D., Soreghan, B., Kwon, M., Kosmoski, J., Knauer, M., Henschen, A., Yates, J., Cotman, C., & Glabe, C.
- Affiliations
- University of California, Irvine (US)
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1992-01
- Volume
- 267
- Issue
- 1
- Pages
- 546-554
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2130144581
- Cited by
- 1,014
- References
- 42
- Citations by year
- 2026: 7 · 2025: 8 · 2024: 14 · 2023: 21 · 2022: 14 · 2021: 29 · 2020: 20 · 2019: 22 · 2018: 28 · 2017: 27 · 2016: 30 · 2015: 29 · 2014: 37 · 2013: 29 · 2012: 31
- FWCI
- 28.77
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Biological Chemistry (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (1r35ag007918-01, 5r01hd021379-14)
- National Institute on Aging (AG07918)
- Eunice Kennedy Shriver National Institute of Child Health and Human Development (HD21379)
- Topics
- Health Sciences › Medicine › Physiology › Alzheimer's disease research and treatments
- Health Sciences › Medicine › Psychiatry and Mental health › Dementia and Cognitive Impairment Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Prion Diseases and Protein Misfolding
- Keywords
- amyloid-beta, Alzheimer's disease, peptide analogues, amyloid fibril formation, peptide aggregation, solubility, pH dependence, Congo red staining, thioflavin T staining, amyloid plaques, hexafluoroisopropanol, amyloid deposition
- MeSH terms
- Alzheimer Disease, Amino Acid Sequence, Amyloid, Chromatography, High Pressure Liquid, Electrophoresis, Polyacrylamide Gel, Hydrogen-Ion Concentration, Kinetics, Microscopy, Electron, Molecular Sequence Data, Peptides, Mass Spectrometry, Amyloid beta-Peptides
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