Cited by 157Cholesterol and Lipid MetabolismLipoproteins and Cardiovascular HealthLipid metabolism and disorders

Structural and metabolic heterogeneity of beta-very low density lipoproteins from cholesterol-fed dogs and from humans with type III hyperlipoproteinemia.

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Fainaru, M, Mahley, R W, Hamilton, R L, Innerarity, T L·1982·Journal of Lipid Research

Abstract

Cholesteryl ester-rich beta-very low density lipoproteins (beta-VLDL) are beta-migrating lipoproteins that accumulate in the d < 1.006 g/ml fraction of plasma from cholesterol-fed animals and from patients with Type III hyperlipoproteinemia. They can be separated from pre-beta-migrating very low density lipoproteins in the d 1.006 g/ml fraction by Geon-Pevikon block electrophoresis. The beta-VLDL have a general property of stimulating cholesteryl ester synthesis and accumulation in macrophages. In the present study, we demonstrated that beta-VLDL obtained from cholesterol-fed dogs fasted for 16 hr were heterogeneous and that two subpopulations of particles, referred to as Fractions I and II, could be isolated from the whole beta-VLDL fraction using gel filtration chromatography. These fractions of beta-VLDL were similar in that both were cholesteryl ester rich, had beta-electrophoretic mobility on Geon-Pevikon electrophoresis, and possessed the B and E apoproteins as major constituents. However, Fractions I and II differed in size, shape, electrophoretic mobility, chemical composition, and apoprotein B type. (Fraction I vs. Fraction II: size: 90 to 300 nm vs. 20 to 70 nm; shape: irregular with redundant surface vs. spherical; electrophoretic mobility on paper: origin vs. beta; chemical composition: rich in phospholipid and poor in protein vs. rich in protein and poor in triglycerides; apoprotein B types: equal amounts of the high and low molecular weight forms vs. predominant…

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Crossref · 10.1016/s0022-2275(20)38102-5

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  • Defective binding of apoE mutants to lipoprotein receptors causes impaired clearance of chylomicron and very low density lipoprotein remnants (β-VLDL), which accumulate in plasma and cause hypercholesterolemia and hypertriglyceridemia.

    “This defective binding causes impaired clearance of chylomicron and very low density lipoprotein remnants (β-VLDL) (5, 6), which accumulate in the plasma and cause both hypercholesterolemia and hypertriglyceridemia.”

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Authors
Fainaru, M., Mahley, R. W., Hamilton, R. L., & Innerarity, T. L.
Affiliations
Gladstone Institutes (US)
Venue
Journal of Lipid Research
Publisher
Elsevier BV
Published
1982-07
Volume
23
Issue
5
Pages
702-714
ISSN
0022-2275 (ISSN-L)
OpenAlex
W2336112818
Cited by
156
References
29
Citations by year
2023: 1 · 2020: 1
FWCI
34.22
Type
journal article
Language
en
Open access
Locations
  • Journal of Lipid Research (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Topics
  • Health Sciences › Medicine › Surgery › Cholesterol and Lipid Metabolism
  • Health Sciences › Medicine › Surgery › Lipoproteins and Cardiovascular Health
  • Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Lipid metabolism and disorders
Keywords
beta-VLDL, chylomicron remnants, type III hyperlipoproteinemia, apolipoprotein B, lipoprotein heterogeneity, cholesteryl ester synthesis, macrophages, retinol transport, lipoprotein clearance, fasting, lipoprotein metabolism, size exclusion chromatography
MeSH terms
Animals, Cells, Cultured, Cholesterol, Dietary, Chromatography, Gel, Dogs, Electrophoresis, Polyacrylamide Gel, Fasting, Female, Humans, Hyperlipoproteinemia Type III, Lipoproteins, VLDL, Male, Microscopy, Electron, Vitamin A, Mice

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