Fatty acid amide hydrolase is located preferentially in large neurons in the rat central nervous system as revealed by immunohistochemistry
Abstract
The distribution in the rat brain of fatty acid amide hydrolase (FAAH) an enzyme that catalyzes the hydrolysis of the endogenous cannabinoid anandamide was studied by immunohistochemistry. An immunopurified, polyclonal antibody to the C terminal region of FAAH was used in these studies. The large principal neurons, such as pyramidal cells in the cerebral cortex, the pyramidal cells the hippocampus, Purkinje cells in the cerebellar cortex and the mitral cells in the olfactory bulb, showed the strongest FAAH immunoreactivity. These FAAH-containing principal neurons except the mitral cells in the olfactory bulb are in close proximity with cannabinoid CB1 receptors as revealed by our previous immunohistochemical study. Moderately or lightly stained FAAH-containing neurons were also found in the amygdala, the basal ganglia, the deep cerebellar nuclei, the ventral posterior nuclei of the thalamus, the optic layer and the intermediate white layer of the superior colliculus and the red nucleus in the midbrain, and motor neurons of the spinal cord. These data demonstrate that FAAH is heterogeneously distributed and this distribution exhibits considerable, although not complete, overlap with the distribution of cannabinoid CB1 receptors in rat brain.
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Within amygdala subnuclei, FAAH is highly expressed on pyramidal neurons in the BLA, and to a significantly lesser extent in the central nucleus (CeA).
“Within amygdala subnuclei, FAAH is highly expressed on pyramidal neurons in the BLA, and to a significantly lesser extent in the central nucleus (CeA) [19,20,46,47].”
From Amygdala FAAH and anandamide: mediating protection and recovery from stress (2013) · cites this paper as [46]
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“Besides the brain, the spinal cord and dorsal root ganglia also express FAAH [12,37,38].”
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- Authors
- Tsou, K., Nogueron, M. I., Muthian, S., Sañudo-Peña, M. C., Hillard, C. J., Deutsch, D. G., & Walker, J. M.
- Affiliations
- Brown University (US)
- Medical College of Wisconsin (US)
- Stony Brook University (US)
- Corresponding author
- Tsou, Kang
- ORCID
- Hillard, Cecilia J
- Venue
- Neuroscience Letters
- Publisher
- Elsevier BV
- Published
- 1998-10
- Volume
- 254
- Issue
- 3
- Pages
- 137-140
- ISSN
- 0304-3940 (ISSN-L)
- OpenAlex
- W1997203123
- Cited by
- 240
- References
- 15
- Citations by year
- 2026: 3 · 2025: 2 · 2024: 4 · 2023: 5 · 2022: 3 · 2020: 2 · 2019: 4 · 2018: 2 · 2017: 6 · 2016: 13 · 2015: 10 · 2014: 8 · 2013: 10 · 2012: 9
- FWCI
- 6.74
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Neuroscience Letters (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (5k02mh001083-03, 5r01ns033247-08, 1r01da010536-01a1)
- National Institute of Mental Health (K02MH01083)
- National Institute on Drug Abuse (DA10536)
- National Institute of Neurological Disorders and Stroke (NS 33247)
- Topics
- Health Sciences › Medicine › Pharmacology › Cannabis and Cannabinoid Research
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Eicosanoids and Hypertension Pharmacology
- Keywords
- fatty acid amide hydrolase, anandamide, endocannabinoid system, CB1 receptor, neuronal distribution, pyramidal neurons, Purkinje cells, mitral cells, amygdala, basal ganglia, red nucleus, spinal motoneurons, rat brain
- MeSH terms
- Fatty Acid Amide Hydrolases, Amidohydrolases, Animals, Brain, Cerebellar Cortex, Hippocampus, Immunohistochemistry, Male, Neurons, Olfactory Bulb, Organ Specificity, Receptors, Drug, Rats, Sprague-Dawley, Pyramidal Cells, Neocortex, Receptors, Cannabinoid, Rats
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