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PCR-based diagnostics for infectious diseases: uses, limitations, and future applications in acute-care settings

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Yang, Samuel, Rothman, Richard E·2004·The Lancet Infectious Diseases
DOI 10.1016/s1473-3099(04)01044-8
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Abstract

Summary Molecular diagnostics are revolutionising the clinical practice of infectious disease. Their effects will be significant in acute-care settings where timely and accurate diagnostic tools are critical for patient treatment decisions and outcomes. PCR is the most well-developed molecular technique up to now, and has a wide range of already fulfilled, and potential, clinical applications, including specific or broad-spectrum pathogen detection, evaluation of emerging novel infections, surveillance, early detection of biothreat agents, and antimicrobial resistance profiling. PCR-based methods may also be cost effective relative to traditional testing procedures. Further advancement of technology is needed to improve automation, optimise detection sensitivity and specificity, and expand the capacity to detect multiple targets simultaneously (multiplexing). This review provides an up-to-date look at the general principles, diagnostic value, and limitations of the most current PCR-based platforms as they evolve from bench to bedside.

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Cited by

See all 1,112

Metadata

Authors
Yang, S., & Rothman, R. E.
Venue
The Lancet Infectious Diseases
Publisher
Elsevier BV
Published
2004-06
Volume
4
Issue
6
Pages
337-348
ISSN
1473-3099 (ISSN-L)
DOI
10.1016/s1473-3099(04)01044-8
OpenAlex
W2167903799
Cited by
1,112
References
4
FWCI
12.92
Type
journal article
Language
en
Open access
bronze
Licence
  • www.elsevier.com/tdm/userlicense/1.0/ (tdm)
  • www.elsevier.com/legal/tdmrep-license (tdm)

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This page
CoChat. (2026). Citation check: PCR-based diagnostics for infectious diseases: uses, limitations, and future applications in acute-care settings. https://app.cochat.ai/papers/10.1016/s1473-3099(04)01044-8
The paper
Yang, S., & Rothman, R. E. (2004). PCR-based diagnostics for infectious diseases: uses, limitations, and future applications in acute-care settings. The Lancet Infectious Diseases. https://doi.org/10.1016/s1473-3099(04)01044-8

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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.

Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.