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Cited by 262Microbial Metabolites in Food BiotechnologyGut microbiota and healthProbiotics and Fermented Foods

Xylo-oligosaccharide (XOS) in combination with inulin modulates both the intestinal environment and immune status in healthy subjects, while XOS alone only shows prebiotic properties

Open access
Lecerf, Jean-Michel, Dépeint, Flore, Clerc, Elise, Dugenet, Yann, Niamba, Claude N., Rhazi, Larbi, Cayzeele, Amélie, Abdelnour, Ghenwa +6 more·2012·British Journal of Nutrition
DOI 10.1017/s0007114511007252
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Abstract

The purpose of the present study was to establish the prebiotic effect of a new xylo-oligosaccharide (XOS) and of an inulin-and-XOS mixture (INU–XOS) and to determine their effect on endotoxaemia (lipopolysaccharides (LPS)) and immune parameters. In this randomised, parallel, placebo-controlled, double-blind study, sixty healthy volunteers were randomly assigned to three groups, receiving either 5 g XOS, INU–XOS (3 g inulin +1 g XOS) or an equivalent weight of wheat maltodextrin (placebo) during 4 weeks. Faecal samples were collected to assess the effects of these products on microbiota, as well as SCFA composition, enzymatic activities and secretory IgA production. Circulating LPS was measured in plasma samples, and whole blood was incubated with LPS to measure cytokine expression. Consumption of XOS alone increased the faecal concentrations of Bifidobacterium and butyrate and activities of α-glucosidase and β-glucuronidase, while decreasing the concentrations of acetate and p-cresol. Consumption of XOS in combination with inulin did not decrease the concentrations of acetate and p-cresol, but increased in addition the faecal concentrations of total SCFA and propionate. Furthermore, consumption of XOS in combination with inulin decreased LPS concentrations in blood and attenuated LPS-induced increases in gene expression in IL-1β and LPS-induced decreases in gene expression in IL-13 in blood. In conclusion, consumption of XOS alone or in combination with inulin results in beneficial albeit different changes in the intestinal microbiome on a high-fat diet. In addition, consumption of XOS in combination with inulin attenuates the proinflammatory effects of a high-fat diet in the blood of healthy subjects.

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Crossref · 10.1017/s0007114511007252

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  • Few in vitro or in vivo studies have examined the interactive beneficial microbiome or human health effects of combining inulin with other prebiotic or fiber substrates.

    “Few in vitro or in vivo studies have examined the interactive beneficial microbiome or human health effects of combining inulin with other prebiotic or fiber substates, though these interactions are likely to be very influential on their behavior in the gut.”

    From Botanicals impact the bifidogenic effect and metabolic outputs of <i>in vitro</i> fiber fermentation by gut-derived microbiota in individual-specific ways (2025) · cites this paper as Lecerf et al., 2012

    The Discussion reports beneficial microbiome and immune effects from combining inulin with XOS and calls this, to the authors’ knowledge, the first clinical study showing anti-inflammatory potential from a mixture of prebiotic fibres. That supports the existence of a relevant study and a narrower claim of clinical novelty, but it does not establish that few in vitro or in vivo studies have examined inulin combined with other prebiotic or fibre substrates generally. (LLM, conf=0.84)

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  • Both prior studies indicate potentially beneficial effects of combining inulin and other prebiotic fibers, ranging from increased SCFAs to attenuation of pro-inflammatory responses.

    “Both of these studies indicate potentially beneficial effects of combining inulin and other prebiotic fibers ranging from increased SCFAs (Koecher et al., 2014) to attenuation of pro-inflammatory responses (Lecerf et al., 2012).”

    From Botanicals impact the bifidogenic effect and metabolic outputs of <i>in vitro</i> fiber fermentation by gut-derived microbiota in individual-specific ways (2025) · cites this paper as Lecerf et al., 2012

  • Research efforts to assess the interactive microbiome or health effect of multiple microbiome-active fibers is still in its infancy.

    “However, research efforts to assess the interactive microbiome or health effect of multiple microbiome-active fibers is still in its infancy.”

    From Botanicals impact the bifidogenic effect and metabolic outputs of <i>in vitro</i> fiber fermentation by gut-derived microbiota in individual-specific ways (2025) · cites this paper as Lecerf et al., 2012

    The Abstract describes a study of XOS combined with inulin, and the Discussion compares its effects with prior studies. Neither section says that research on the interactive effects of multiple microbiome-active fibers is still in its infancy. Searches for limited research on fiber combinations found no passage making that broader assessment. (LLM, conf=0.72)

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Cites

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Research works printed but not linked: 4

  1. Roberfroid MB, Bornet F, Bouley C, et al. (1995) Colonic microflora: nutrition and health. Summary and conclusions of an International Life Sciences Institute (ILSI) [Europe] workshop held in Barcelona, Spain. Nutr Rev 53, 127-130.
  2. Cloetens L (2009) In vivo evaluation of the gastrointestinal effects of arabinoxylan-oligosaccharides in healthy subjects. PhD Thesis, Katholieke Universiteit Leuven.
  3. Pool-Zobel BL (2005) Inulin-type fructans and reduction in colon cancer risk: review of experimental and human data. Br J Nutr 93, Suppl. 1, S73 -S90.
  4. Gibson GR & Macfarlane GT (1995) Human colonic bacteria: role in nutrition, physiology and pathology. In Human Colo- nic Bacteria: Role in Nutrition, Physiology and Pathology, pp. 75 -100 [GR Gibson and GT Macfarlane, editors]. Boca Raton, FL: CRC Press.

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Authors
Lecerf, J.-M., Dépeint, F., Clerc, E., Dugenet, Y., Niamba, C. N., Rhazi, L., Cayzeele, A., Abdelnour, G., Jaruga, A., Younes, H., Jacobs, H., Lambrey, G., Abdelnour, A. M., & Pouillart, P. R.
Affiliations
  • Institut Pasteur de Lille (FR)
  • Institut polytechnique LaSalle Beauvais (FR)
  • BioActor (Netherlands) (NL)
  • Centre Hospitalier de Beauvais (FR)
Corresponding author
Lecerf, Jean-Michel
ORCID
Venue
British Journal of Nutrition
Publisher
Cambridge University Press (CUP)
Published
2012-01-23
Volume
108
Issue
10
Pages
1847-1858
ISSN
  • 0007-1145 (ISSN-L)
  • 1475-2662
DOI
10.1017/s0007114511007252
OpenAlex
W2014368597
Cited by
262
References
6
Citations by year
2026: 6 · 2025: 15 · 2024: 9 · 2023: 11 · 2022: 26 · 2021: 25 · 2020: 27 · 2019: 22 · 2018: 17 · 2017: 25 · 2016: 24 · 2015: 23 · 2014: 18 · 2013: 9 · 2012: 4
FWCI
15.15
Type
journal article
Language
en
Open access
Licence
www.cambridge.org/core/terms (unspecified)
Locations
  • British Journal Of Nutrition (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Nursing › Nutrition and Dietetics › Microbial Metabolites in Food Biotechnology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
Keywords
xylooligosaccharides, inulin, prebiotics, gut microbiota, short-chain fatty acids, endotoxemia, lipopolysaccharide, secretory IgA, Bifidobacterium, butyrate, high-fat diet, inflammatory cytokines, IL-1β, IL-13, p-cresol, alpha-glucosidase, beta-glucuronidase, immune status
MeSH terms
Adolescent, Animals, Bifidobacterium, Double-Blind Method, Drug Combinations, Feces, Female, Humans, Inulin, Male, Oligosaccharides, Dietary Supplements, Young Adult, Prebiotics

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