Ex Vivo Culture of Primary Intestinal Stem Cells in Collagen Gels and Foams
Abstract
Three-dimensional ex vivo cell culture systems have been of great interest to tissue engineering and regenerative medicine. In vitro intestinal epithelium culture models are normally employed to mimics the physiological realistic environment for the study of normal and aberrant intestinal epithelium. In this study, we demonstrated the applicability to use collagen I based gels for the culture of primary intestinal stem cells. The resulting three-dimensional cell culture systems supported the long-term proliferation and differentiation of encapsulated intestinal stem cells. We further confirmed the feasibility of collagen I foams as cell carriers for the delivery of intestinal stem cells. These ex vivo systems will facilitate the research of intestinal stem-cell-based regenerative therapy for enteric disorders and diseases.
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Natural matrices can be recombinantly produced from proteins or polysaccharides to address Matrigel's batch-to-batch variability.
“Natural matrices can be recombinantly produced from proteins or polysaccharides to address the batch-to-batch variability of Matrigel61.”
From Organoids (2022) · cites this paper as [61]
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- Authors
- Peng, H., Poovaiah, N., Forrester, M., Cochran, E., & Wang, Q.
- Affiliations
- Harbin Medical University (CN)
- Iowa State University (US)
- Corresponding author
- Wang, Qun
- Venue
- ACS Biomaterials Science & Engineering
- Publisher
- American Chemical Society (ACS)
- Published
- 2014-12-02
- Volume
- 1
- Issue
- 1
- Pages
- 37-42
- ISSN
- 2373-9878 (ISSN-L)
- OpenAlex
- W2322967688
- Cited by
- 55
- References
- 35
- Citations by year
- 2026: 1 · 2025: 4 · 2024: 4 · 2023: 10 · 2022: 3 · 2021: 2 · 2020: 4 · 2019: 7 · 2018: 13 · 2017: 3 · 2016: 1 · 2015: 3
- FWCI
- 2.31
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- ACS Biomaterials Science & Engineering (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Office of the President, Iowa State University
- Topics
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Hydrogels: synthesis, properties, applications
- Keywords
- intestinal stem cells, ex vivo culture, collagen I gel, 3D cell culture, intestinal epithelium, cell encapsulation, cell carriers, stem cell delivery, stem cell proliferation and differentiation
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