The DOI matches its Crossref record; 31 of the 31 references it cites were found in a registry or an index.
Multiplexed Nanoflares: mRNA Detection in Live Cells
Open accessAbstract
We report the development of the multiplexed nanoflare, a nanoparticle agent that is capable of simultaneously detecting two distinct mRNA targets inside a living cell. These probes are spherical nucleic acid (SNA) gold nanoparticle (Au NP) conjugates consisting of densely packed and highly oriented oligonucleotide sequences, many of which are hybridized to a reporter with a distinct fluorophore label and each complementary to its corresponding mRNA target. When multiplexed nanoflares are exposed to their targets, they provide a sequence specific signal in both extra- and intracellular environments. Importantly, one of the targets can be used as an internal control, improving detection by accounting for cell-to-cell variations in nanoparticle uptake and background. Compared to single-component nanoflares, these structures allow one to determine more precisely relative mRNA levels in individual cells, improving cell sorting and quantification.
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Metadata
- Authors
- Prigodich, A. E., Randeria, P. S., Briley, W. E., Kim, N. J., Daniel, W. L., Giljohann, D. A., & Mirkin, C. A.
- Venue
- Analytical Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2012-01-30
- Volume
- 84
- Issue
- 4
- Pages
- 2062-2066
- ISSN
- 0003-2700 (ISSN-L)
- 1520-6882
- OpenAlex
- W2137158680
- Cited by
- 285
- References
- 31
- FWCI
- 10.92
- Type
- journal article
- Language
- en
- Open access
- green
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