Ultrasensitive Sample Quantitation via Selected Reaction Monitoring Using CITP/CZE–ESI-Triple Quadrupole MS
Open accessAbstract
We demonstrate the direct coupling of transient capillary isotachophoresis/capillary zone electrophoresis (CITP/CZE) with a high-sensitivity triple quadrupole mass spectrometer operating in selected reaction monitoring (SRM) mode for sample quantitation. The capability of CITP/CZE for in situ sample enrichment and separation has been shown to significantly improve the analytical figures of merit. A linear dynamic range spanning 4 orders of magnitude was observed. An average signal-to-noise ratio (S/N) of 49.6 was observed for 50 amol of targeted peptide in the presence of a complex and much more abundant bovine serum albumin (BSA) digest. Correlation of variation (CV) of <10% for peak area was measured from triplicate sample analyses at 50 pM peptide concentration, showing good reproducibility of this online CITP/CZE-SRM mass spectrometry (MS) platform, and with limit of quantitation (LOQ) demonstrated to be well below 50 pM.
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tITP requires optimization of both electrolyte concentrations and loading volume for efficient stacking of target analytes.
“tITP requires an optimization of both electrolyte concentrations and loading volume to enable efficient stacking of the target analytes13,27,65,120–122.”
From Recent trends of capillary electrophoresis‐mass spectrometry in proteomics research (2019) · cites this paper as [120]
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Cites
On-line mass spectrometric detection for capillary zone electrophoresis
Olivares, J. A., Nhung, N. T., Yonker, C. R., & Smith, R. D. (1987) · Analytical Chemistry · doi:10.1021/ac00135a034 · Cited by 635 · see citing works
Self-citationImproved electrospray ionization interface for capillary zone electrophoresis-mass spectrometry
Smith, R. D., Barinaga, C. J., & Udseth, H. R. (1988) · Analytical Chemistry · doi:10.1021/ac00169a022 · Cited by 534 · see citing works
Self-citationKuzyk, M. A., Smith, D., Yang, J., Cross, T. J., Jackson, A. M., Hardie, D. B., Anderson, N. L., & Borchers, C. H. (2009) · Molecular & Cellular Proteomics · doi:10.1074/mcp.m800540-mcp200 · Cited by 515 · see citing works
Capillary zone electrophoresis-mass spectrometry using an electrospray ionization interface
Smith, R. D., Olivares, J. A., Nhung, N. T., & Udseth, H. R. (1988) · Analytical Chemistry · doi:10.1021/ac00156a013 · Cited by 393 · see citing works
Self-citationMoini, M. (2007) · Analytical Chemistry · doi:10.1021/ac0704560 · Cited by 322 · see citing works
Metabolome analysis by capillary electrophoresis–mass spectrometry
Monton, M. R. N., & Soga, T. (2007) · Journal of Chromatography A · doi:10.1016/j.chroma.2007.02.065 · Cited by 319 · see citing works
Kelly, R., Page, J. S., Luo, Q., et al. (2006) · Analytical Chemistry · doi:10.1021/ac061133r · Cited by 242 · see citing works
Lee, E. D., Mück, W. M., Henion, J. D., & Covey, T. R. (1988) · Journal of Chromatography A · doi:10.1016/s0021-9673(00)90575-2 · Cited by 209 · see citing works
Relevance and use of capillary coatings in capillary electrophoresis–mass spectrometry
Hühn, C., Ramautar, R., Wuhrer, M., & Somsen, G. W. (2009) · Analytical and Bioanalytical Chemistry · doi:10.1007/s00216-009-3193-y · Cited by 196 · see citing works
Busnel, J., Schoenmaker, B., Ramautar, R., Carrasco‐Pancorbo, A., Ratnayake, C. K., Feitelson, J. S., Chapman, J. D., Deelder, A. M., & Mayboroda, O. A. (2010) · Analytical Chemistry · doi:10.1021/ac102159d · Cited by 165 · see citing works
Recent advances of capillary electrophoresis in pharmaceutical analysis
Suntornsuk, L. (2010) · Analytical and Bioanalytical Chemistry · doi:10.1007/s00216-010-3741-5 · Cited by 164 · see citing works
Decoupling CE and ESI for a more robust interface with MS
Maxwell, E. J., Zhong, X., Zhang, H., van Zeijl, N., & Chen, D. D. Y. (2010) · Electrophoresis · doi:10.1002/elps.200900517 · Cited by 150 · see citing works
Isotachophoresis as an on-line concentration pretreatment technique in capillary electrophoresis
Stegehuis, D. S., Irthu, H., Tjaden, U. R., & van der Greef, J. (1991) · Journal of Chromatography A · doi:10.1016/s0021-9673(01)88860-9 · Cited by 148 · see citing works
Ramautar, R., Busnel, J., Deelder, A. M., & Mayboroda, O. A. (2011) · Analytical Chemistry · doi:10.1021/ac202407v · Cited by 129 · see citing works
Targeted mass spectrometry approaches for protein biomarker verification
Meng, Z., & Veenstra, T. D. (2011) · Journal of Proteomics · doi:10.1016/j.jprot.2011.04.011 · Cited by 107 · see citing works
CE‐MS in biomarker discovery, validation, and clinical application
Mischak, H., & Schanstra, J. P. (2010) · PROTEOMICS - CLINICAL APPLICATIONS · doi:10.1002/prca.201000058 · Cited by 102 · see citing works
Barbas, C., Moraes, E. P., & Villaseñor, A. (2011) · Journal of Pharmaceutical and Biomedical Analysis · doi:10.1016/j.jpba.2011.02.001 · Cited by 101 · see citing works
Heemskerk, A. A. M., Busnel, J., Schoenmaker, B., Derks, R. J. E., Klychnikov, O. I., Hensbergen, P. J., Deelder, A. M., & Mayboroda, O. A. (2012) · Analytical Chemistry · doi:10.1021/ac300641x · Cited by 100 · see citing works
Improving mass spectrometer sensitivity using a high-pressure electrodynamic ion funnel interface
Ibrahim, Y., Tang, K., Tolmachev, A. V., et al. (2006) · Journal of the American Society for Mass Spectrometry · doi:10.1016/j.jasms.2006.06.005 · Cited by 98 · see citing works
Self-citationKuhr, W. G. (1990) · Analytical Chemistry · doi:10.1021/ac00211a027 · Cited by 91 · see citing works
CE–MS for proteomics: Advances in interface development and application
Ramautar, R., Heemskerk, A. A. M., Hensbergen, P. J., Deelder, A. M., Busnel, J.-M., & Mayboroda, O. A. (2012) · Journal of Proteomics · doi:10.1016/j.jprot.2012.04.050 · Cited by 86 · see citing works
Mischak, H., Delles, C., Klein, J., & Schanstra, J. P. (2010) · Advances in Chronic Kidney Disease · doi:10.1053/j.ackd.2010.09.004 · Cited by 78 · see citing works
Fang, X., Li, Y., Wang, W., Song, T., Lee, C. S., DeVoe, D. L., & Balgley, B. M. (2007) · Analytical Chemistry · doi:10.1021/ac070611a · Cited by 72 · see citing works
Self-citationHaselberg, R., de Jong, G. J., & Somsen, G. W. (2010) · Analytica Chimica Acta · doi:10.1016/j.aca.2010.08.032 · Cited by 64 · see citing works
The application of CE‐MS in the trace analysis of environmental pollutants and food contaminants
Robledo, V. R., & Smyth, W. F. (2009) · Electrophoresis · doi:10.1002/elps.200800651 · Cited by 61 · see citing works
Li, Y., Wojcik, R., Dovic̀hi, N. J., & Champion, M. M. (2012) · Analytical Chemistry · doi:10.1021/ac300926h · Cited by 52 · see citing works
Analysis of endogenous glutathione‐adducts and their metabolites
Blair, I. A. (2009) · Biomedical Chromatography · doi:10.1002/bmc.1374 · Cited by 51 · see citing works
Verification of Biomarkers for Diabetic Retinopathy by Multiple Reaction Monitoring
Kim, K., Kim, S. J., Yu, H. G., Yu, J., Park, K. S., Jang, I., & Kim, Y. (2009) · Journal of Proteome Research · doi:10.1021/pr901013d · Cited by 50 · see citing works
An, Y., Cooper, J. W., Balgley, B. M., & Lee, C. S. (2006) · Electrophoresis · doi:10.1002/elps.200600093 · Cited by 50 · see citing works
Self-citationRangiah, K., Hwang, W., Mesaros, C., et al. (2011) · Bioanalysis · doi:10.4155/bio.11.42 · Cited by 49 · see citing works
Capillary electrophoresis–mass spectrometry: Recent trends in clinical proteomics
Desiderio, C., Rossetti, D. V., Iavarone, F., Messana, I., & Castagnola, M. (2010) · Journal of Pharmaceutical and Biomedical Analysis · doi:10.1016/j.jpba.2010.06.035 · Cited by 47 · see citing works
Recent food safety and food quality applications of CE‐MS
Ravelo‐Pérez, L. M., Asensio‐Ramos, M., Hernández‐Borges, J., & Rodríguez‐Delgado, M. Á. (2009) · Electrophoresis · doi:10.1002/elps.200800670 · Cited by 35 · see citing works
Jeong, J., Kim, S., & Park, S. (2012) · Electrophoresis · doi:10.1002/elps.201200005 · Cited by 34 · see citing works
Fang, X., Wang, W., Yang, L., Chandrasekaran, K., Kristián, T., Balgley, B. M., & Lee, C. S. (2008) · Electrophoresis · doi:10.1002/elps.200700609 · Cited by 33 · see citing works
Self-citationLiu, Y., Fu, X., Bai, Y., et al. (2011) · Analytical and Bioanalytical Chemistry · doi:10.1007/s00216-011-4659-2 · Cited by 28 · see citing works
Growing trend of CE at the omics level: The frontier of systems biology – An update
Ban, E., Park, S. H., Kang, M., Lee, H., Song, E. J., & Yoo, Y. S. (2011) · Electrophoresis · doi:10.1002/elps.201100344 · Cited by 27 · see citing works
CE‐MS and related techniques as a valuable tool in tumor biomarkers research
Simionato, A. V. C., Carrilho, E., & Tavares, M. F. M. (2010) · Electrophoresis · doi:10.1002/elps.200900671 · Cited by 22 · see citing works
Biomarker Analysis for Prostate Cancer Diagnosis Using LC–MS and CE–MS
Gamagedara, S., & Ma, Y. (2011) · Bioanalysis · doi:10.4155/bio.11.203 · Cited by 16 · see citing works
Flagged first, then by citations
Research works printed but not linked: 2
- [8]Ravelo-Perez LM Asensio-Ramos M Hernandez-Borges J Rodriguez-Delgado MA Electrophoresis 2009 30 1624 19360778
- [36]Kuhr WG Anal Chem 1990 62 403R 20527859
Cited by
Sorted by citations
Recent trends of capillary electrophoresis‐mass spectrometry in proteomics research
Gomes, F. P., & Yates, J. R. (2019) · Mass Spectrometry Reviews · doi:10.1002/mas.21599 · Cited by 112 · see citing works
CE‐MS‐based proteomics in biomarker discovery and clinical application
Pontillo, C., Filip, S., Borràs, D. M., Mullen, W., Vlahou, A., & Mischak, H. (2015) · PROTEOMICS – Clinical Applications · doi:10.1002/prca.201400115 · Cited by 77 · see citing works
Capillary zone electrophoresis-mass spectrometry for bottom-up proteomics
Zhang, Z., Qu, Y., & Dovichi, N. J. (2018) · TrAC Trends in Analytical Chemistry · doi:10.1016/j.trac.2018.08.008 · Cited by 61 · see citing works
CE–ESI‐MS for bottom‐up proteomics: Advances in separation, interfacing and applications
Heemskerk, A. A. M., Deelder, A. M., & Mayboroda, O. A. (2016) · Mass Spectrometry Reviews · doi:10.1002/mas.21432 · Cited by 61 · see citing works
Guo, X., Fillmore, T. L., Gao, Y., & Tang, K. (2016) · Analytical Chemistry · doi:10.1021/acs.analchem.5b04912 · Cited by 54 · see citing works
Self-citationWang, C., Lee, C. S., Smith, R. D., & Tang, K. (2013) · Analytical Chemistry · doi:10.1021/ac401202c · Cited by 46 · see citing works
Self-citationRecent advances of capillary electrophoresis-mass spectrometry instrumentation and methodology
Jiang, Y., He, M.-Y., Zhang, W.-J., Luo, P., Guo, D., Fang, X., & Xu, W. (2017) · Chinese Chemical Letters · doi:10.1016/j.cclet.2017.05.008 · Cited by 30 · see citing works
- Authors
- Wang, C., Lee, C. S., Smith, R. D., & Tang, K.
- Affiliations
- University of Maryland, College Park (US)
- Pacific Northwest National Laboratory (US)
- Corresponding author
- Tang, Keqi
- Venue
- Analytical Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2012-11-20
- Volume
- 84
- Issue
- 23
- Pages
- 10395-10403
- ISSN
- 0003-2700 (ISSN-L)
- 1520-6882
- PMC
- PMC3547498
- OpenAlex
- W2086757556
- Cited by
- 38
- References
- 38
- Citations by year
- 2024: 2 · 2020: 5 · 2019: 1 · 2018: 5 · 2017: 7 · 2016: 4 · 2015: 2 · 2014: 7 · 2013: 4 · 2012: 1
- FWCI
- 2.55
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Analytical Chemistry (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- U.S. Department of Energy
- Battelle
- National Institutes of Health (5p41gm103493-20)
- National Cancer Institute (R21CA143177, R33CA155252)
- National Institute of General Medical Sciences (8 P41 GM103493-10, R21GM103536, P41-GM103493)
- Pacific Northwest National Laboratory
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Microfluidic and Capillary Electrophoresis Applications
- Physical Sciences › Chemistry › Spectroscopy › Mass Spectrometry Techniques and Applications
- Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
- Keywords
- capillary isotachophoresis, capillary zone electrophoresis, multiple reaction monitoring, triple quadrupole mass spectrometry, peptide quantification, detection limit, signal-to-noise ratio
- MeSH terms
- Animals, Cattle, Humans, Peptide Fragments, Serum Albumin, Bovine, Reproducibility of Results, Electrophoresis, Capillary, Spectrometry, Mass, Electrospray Ionization, Isotachophoresis, Signal-To-Noise Ratio
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