CE–MS for proteomics: Advances in interface development and application
Abstract
Capillary electrophoresis-mass spectrometry (CE-MS) has emerged as a powerful technique for the analysis of proteins and peptides. Over the past few years, significant progress has been made in the development of novel and more effective interfaces for hyphenating CE to MS. This review provides an overview of these new interfacing techniques for coupling CE to MS, covering the scientific literature from January 2007 to December 2011. The potential of these new CE-MS interfacing techniques is demonstrated within the field of (clinical) proteomics, more specifically "bottom-up" proteomics, by showing examples of the analysis of various biological samples. The relevant papers on CE-MS for proteomics are comprehensively summarized in tables, including, e.g. information on sample type and pretreatment, interfacing and MS detection mode. Finally, general conclusions and future perspectives are provided.
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- Authors
- Ramautar, R., Heemskerk, A. A. M., Hensbergen, P. J., Deelder, A. M., Busnel, J.-M., & Mayboroda, O. A.
- Affiliations
- Leiden University Medical Center (NL)
- Corresponding author
- Ramautar, Rawi
- Venue
- Journal of Proteomics
- Publisher
- Elsevier BV
- Published
- 2012-07
- Volume
- 75
- Issue
- 13
- Pages
- 3814-3828
- ISSN
- 1874-3919 (ISSN-L)
- OpenAlex
- W2067978623
- Cited by
- 86
- References
- 94
- Citations by year
- 2026: 2 · 2024: 1 · 2023: 3 · 2022: 5 · 2021: 3 · 2020: 5 · 2019: 4 · 2018: 5 · 2017: 3 · 2016: 9 · 2015: 9 · 2014: 16 · 2013: 17 · 2012: 4
- FWCI
- 1.38
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Proteomics (publishedVersion)
- PubMed (publishedVersion)
- Data Archiving and Networked Services (DANS) (submittedVersion)
- Leiden Repository (Leiden University) (submittedVersion)
- Topics
- Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
- Physical Sciences › Chemistry › Spectroscopy › Mass Spectrometry Techniques and Applications
- Physical Sciences › Engineering › Biomedical Engineering › Microfluidic and Capillary Electrophoresis Applications
- Keywords
- capillary electrophoresis-mass spectrometry, shotgun proteomics, clinical proteomics, protein analysis, peptide analysis, sample preparation, biological specimen
- MeSH terms
- Animals, Humans, Proteins, Mass Spectrometry, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Electrophoresis, Capillary, Proteomics
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