Cited by 86Advanced Proteomics Techniques and ApplicationsMass Spectrometry Techniques and ApplicationsMicrofluidic and Capillary Electrophoresis Applications

CE–MS for proteomics: Advances in interface development and application

Ramautar, Rawi, Heemskerk, Anthonius A.M., Hensbergen, Paul J., Deelder, André M., Busnel, Jean-Marc, Mayboroda, Oleg A.·2012·Journal of Proteomics

Abstract

Capillary electrophoresis-mass spectrometry (CE-MS) has emerged as a powerful technique for the analysis of proteins and peptides. Over the past few years, significant progress has been made in the development of novel and more effective interfaces for hyphenating CE to MS. This review provides an overview of these new interfacing techniques for coupling CE to MS, covering the scientific literature from January 2007 to December 2011. The potential of these new CE-MS interfacing techniques is demonstrated within the field of (clinical) proteomics, more specifically "bottom-up" proteomics, by showing examples of the analysis of various biological samples. The relevant papers on CE-MS for proteomics are comprehensively summarized in tables, including, e.g. information on sample type and pretreatment, interfacing and MS detection mode. Finally, general conclusions and future perspectives are provided.

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Crossref · 10.1016/j.jprot.2012.04.050

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Authors
Ramautar, R., Heemskerk, A. A. M., Hensbergen, P. J., Deelder, A. M., Busnel, J.-M., & Mayboroda, O. A.
Affiliations
Leiden University Medical Center (NL)
Corresponding author
Ramautar, Rawi
Venue
Journal of Proteomics
Publisher
Elsevier BV
Published
2012-07
Volume
75
Issue
13
Pages
3814-3828
ISSN
1874-3919 (ISSN-L)
OpenAlex
W2067978623
Cited by
86
References
94
Citations by year
2026: 2 · 2024: 1 · 2023: 3 · 2022: 5 · 2021: 3 · 2020: 5 · 2019: 4 · 2018: 5 · 2017: 3 · 2016: 9 · 2015: 9 · 2014: 16 · 2013: 17 · 2012: 4
FWCI
1.38
Type
journal article
Language
en
Open access
closed
Locations
  • Journal of Proteomics (publishedVersion)
  • PubMed (publishedVersion)
  • Data Archiving and Networked Services (DANS) (submittedVersion)
  • Leiden Repository (Leiden University) (submittedVersion)
Topics
  • Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
  • Physical Sciences › Chemistry › Spectroscopy › Mass Spectrometry Techniques and Applications
  • Physical Sciences › Engineering › Biomedical Engineering › Microfluidic and Capillary Electrophoresis Applications
Keywords
capillary electrophoresis-mass spectrometry, shotgun proteomics, clinical proteomics, protein analysis, peptide analysis, sample preparation, biological specimen
MeSH terms
Animals, Humans, Proteins, Mass Spectrometry, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Electrophoresis, Capillary, Proteomics

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