Capillary Isotachophoresis-Nanoelectrospray Ionization-Selected Reaction Monitoring MS via a Novel Sheathless Interface for High Sensitivity Sample Quantification

Wang, Chenchen; Lee, Cheng S.; Smith, Richard D.; Tang, Keqi

doi:10.1021/ac401202c

Cited by 46Microfluidic and Capillary Electrophoresis ApplicationsMass Spectrometry Techniques and ApplicationsAdvanced Proteomics Techniques and Applications

Capillary Isotachophoresis-Nanoelectrospray Ionization-Selected Reaction Monitoring MS via a Novel Sheathless Interface for High Sensitivity Sample Quantification

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Wang, Chenchen, Lee, Cheng S., Smith, Richard D., Tang, Keqi·2013·Analytical Chemistry

DOI 10.1021/ac401202cPMC PMC3744340

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Abstract

A novel sheathless capillary isotachophoresis (CITP/CZE)-mass spectrometry (MS) interface featuring a large inner diameter (i.d.) separation capillary, and a detachable small i.d. porous electrospray ionization (ESI) emitter was developed in this study to simultaneously achieve large sample loading capacity and stable nanoESI operation. Crucial operating parameters, including sample loading volume, flow rate, and separation window, were systematically investigated to attain optimum CITP/CZE separation efficiency and MS detection sensitivity. The performance of CITP/CZE-nanoESI-MS using the new sheathless interface was evaluated for its achievable low limit of quantification (LOQ) by analyzing targeted peptides, leu-enkephalin and angiotensin II, spiked in a BSA tryptic digest matrix at different concentrations. A linear dynamic range spanning 4.5 orders of magnitude and a 10 pM LOQ with measurement reproducibility of the CV < 22% were obtained experimentally for both targeted peptides, representing a 5-fold sensitivity improvement as compared to using the sheath liquid interface developed previously.1.

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tITP requires optimization of both electrolyte concentrations and loading volume for efficient stacking of target analytes.
“tITP requires an optimization of both electrolyte concentrations and loading volume to enable efficient stacking of the target analytes13,27,65,120–122.”
From Recent trends of capillary electrophoresis‐mass spectrometry in proteomics research (2019) · cites this paper as [121]
cITP/CZE quantification of targeted BSA tryptic peptides had a quantitation limit of 10 pM and a coefficient of variation lower than 22%.
“They observed a quantitation limit of 10 pM with a CV lower than 22%121.”
From Recent trends of capillary electrophoresis‐mass spectrometry in proteomics research (2019) · cites this paper as [121]

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Authors: Wang, C., Lee, C. S., Smith, R. D., & Tang, K.
Affiliations: University of Maryland, College Park (US)
Pacific Northwest National Laboratory (US)
Corresponding author: Tang, Keqi
ORCID: Wang, Chenchen
Smith, Richard D.
Tang, Keqi
Venue: Analytical Chemistry
Publisher: American Chemical Society (ACS)
Published: 2013-07-08
Volume: 85
Issue: 15
Pages: 7308-7315
ISSN: 0003-2700 (ISSN-L)
1520-6882
DOI: 10.1021/ac401202c
PMC: PMC3744340
OpenAlex: W1995204488
Cited by: 46
References: 41
Citations by year: 2026: 2 · 2024: 1 · 2022: 1 · 2021: 1 · 2020: 5 · 2019: 2 · 2018: 4 · 2017: 7 · 2016: 8 · 2015: 5 · 2014: 9 · 2013: 1
FWCI: 4.22
Type: journal article
Language: en
Open access: green
www.ncbi.nlm.nih.gov
Locations: Analytical Chemistry (publishedVersion)
PubMed (publishedVersion)
PubMed Central (acceptedVersion)
Funders: National Institutes of Health (5p41gm103493-20)
National Cancer Institute (R21CA143177, R33CA155252)
National Institute of General Medical Sciences (8 P41 GM103493-10, R21GM103536, P41-GM103493)
Topics: Physical Sciences › Engineering › Biomedical Engineering › Microfluidic and Capillary Electrophoresis Applications
Physical Sciences › Chemistry › Spectroscopy › Mass Spectrometry Techniques and Applications
Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
Keywords: capillary isotachophoresis, capillary zone electrophoresis, nanoelectrospray ionization, electrospray emitter, multiple reaction monitoring, peptide quantification, leu-enkephalin, angiotensin II, limit of quantification, sample loading capacity, tryptic digest
MeSH terms: Amino Acid Sequence, Animals, Cattle, Humans, Peptides, Spectrometry, Mass, Electrospray Ionization, Nanotechnology, Isotachophoresis

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Capillary Isotachophoresis-Nanoelectrospray Ionization-Selected Reaction Monitoring MS via a Novel Sheathless Interface for High Sensitivity Sample Quantification

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