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Microchip Device for Cell Lysis, Multiplex PCR Amplification, and Electrophoretic Sizing

Waters, Larry C., Jacobson, Stephen C., Kroutchinina, Natalia, Khandurina, Julia, Foote, Robert S., Ramsey, J. Michael·1998·Analytical Chemistry
DOI 10.1021/ac970642d
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Abstract

The steps of cell lysis, multiplex PCR amplification, and electrophoretic analysis are executed sequentially on a monolithic microchip device. The entire microchip is thermally cycled to lyse cells and to amplify DNA, and the products are then analyzed using a sieving medium for size separation and an intercalating dye for fluorescence detection. Using a standard PCR protocol, a 500-base pair (bp) region of bacteriophage lambda DNA and 154-, 264-, 346-, 410-, and 550-bp regions of E. coli genomic and plasmid DNAs are amplified. The electrophoretic analysis of the products is executed in <3 min following amplification using hydroxyethyl cellulose or poly(dimethylacrylamide) sieving gels. Product sizing is demonstrated by proportioning the amplified product with a DNA sizing ladder.

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Metadata

Authors
Waters, L. C., Jacobson, S. C., Kroutchinina, N., Khandurina, J., Foote, R. S., & Ramsey, J. M.
Venue
Analytical Chemistry
Publisher
American Chemical Society (ACS)
Published
1998-01-01
Volume
70
Issue
1
Pages
158-162
ISSN
  • 0003-2700 (ISSN-L)
  • 1520-6882
DOI
10.1021/ac970642d
OpenAlex
W2079112687
Cited by
405
References
0
FWCI
35.10
Type
journal article
Language
en
Open access
closed

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CoChat. (2026). Citation check: Microchip Device for Cell Lysis, Multiplex PCR Amplification, and Electrophoretic Sizing. https://app.cochat.ai/papers/10.1021/ac970642d
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Waters, L. C., Jacobson, S. C., Kroutchinina, N., Khandurina, J., Foote, R. S., & Ramsey, J. M. (1998). Microchip Device for Cell Lysis, Multiplex PCR Amplification, and Electrophoretic Sizing. Analytical Chemistry. https://doi.org/10.1021/ac970642d

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