Citrus Tangeretin Improves Skeletal Muscle Mitochondrial Biogenesis via Activating the AMPK-PGC1-α Pathway In Vitro and In Vivo: A Possible Mechanism for Its Beneficial Effect on Physical Performance
Abstract
Mitochondrial biogenesis is a key factor, which influences the function of skeletal muscle. Increasingly, flavonoids are reported to have the potential ability of regulating mitochondrial biogenesis. In this study, we investigated the effects of tangeretin, a polymethoxylated flavonoid isolated from mandarin fruits, on mitochondrial biogenesis and its underlying mechanisms. The tangeretin was obtained from the peel of "Dahongpao" tangerine by macroporous adsorptive resins combined with preparative-high performance liquid chromatography. The activity of mitochondrial biogenesis was explored by using mouse-derived C2C12 myoblasts and Kunming mice. Results showed that the purity of tangeretin obtained was 98.64%, and it could effectively activate mitochondrial biogenesis signaling pathway both at gene and at protein levels in C2C12 myoblasts. Animal experiments showed that tangeretin pretreatment could markedly improve exercise performance (the time of hanging wire and run to fatigue was obviously increased 1.6-fold and 2.1-fold in the high-dose tangeretin group, respectively), and the transmission electron microscopy, Western blotting, and immunohistochemistry further indicated that tangeretin increased mitochondria number and activated mitochondrial biogenesis signaling axis. Our findings suggest that tangeretin enhanced mitochondrial biogenesis via activating the AMPK-PGC1-α pathway, resulting in the improvement of exercise performance, and tangeretin may be a potentially novel mitochondria regulator in foods.
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Tangeretin increases mitochondrial content.
“Similarly, tangeretin, a flavonoid from the peels of citrus fruits, increases mitochondrial content and activates mitochondrial biogenesis signaling axis via AMPK/PGC1α signaling pathway [99].”
From Mitochondrial Adaptation in Skeletal Muscle: Impact of Obesity, Caloric Restriction, and Dietary Compounds (2024) · cites this paper as [99]
Tangeretin activates the mitochondrial biogenesis signaling axis via the AMPK/PGC1α signaling pathway.
“Similarly, tangeretin, a flavonoid from the peels of citrus fruits, increases mitochondrial content and activates mitochondrial biogenesis signaling axis via AMPK/PGC1α signaling pathway [99].”
From Mitochondrial Adaptation in Skeletal Muscle: Impact of Obesity, Caloric Restriction, and Dietary Compounds (2024) · cites this paper as [99]
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Self-citation
- Authors
- Kou, G., Li, Z., Wu, C., Liu, Y., Hu, Y., Guo, L., Xu, X., & Zhou, Z.
- Affiliations
- Southwest University (CN)
- Corresponding author
- Xu, Xiaoyu
- Zhou, Zhiqin
- Venue
- Journal of Agricultural and Food Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2018-10-28
- Volume
- 66
- Issue
- 45
- Pages
- 11917-11925
- ISSN
- 0021-8561 (ISSN-L)
- 1520-5118
- OpenAlex
- W2898547892
- Cited by
- 78
- References
- 36
- Citations by year
- 2026: 9 · 2025: 14 · 2024: 16 · 2023: 7 · 2022: 13 · 2021: 9 · 2020: 9 · 2019: 1
- FWCI
- 4.20
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Agricultural and Food Chemistry (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of China (31772260)
- National Risk Assessment Program for Agricultural Products Quality and Safety (GJFP201801502)
- Chongqing Program for Production of Late Maturing Citrus Fruits and Plums (2018-01-4)
- Chongqing Graduate Student Research Innovation Project (CYB 18081)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Bioactive Compounds in Plants
- Health Sciences › Medicine › Rehabilitation › Exercise and Physiological Responses
- Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
- Keywords
- tangeretin, citrus flavonoids, mitochondrial biogenesis, skeletal muscle, exercise performance, C2C12 myoblasts, tangerine peel, flavonoid purification, fatigue resistance
- MeSH terms
- Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha, Animals, Organelle Biogenesis, Cell Differentiation, Citrus, Male, Mitochondria, Physical Conditioning, Animal, Plant Extracts, Muscle, Skeletal, Myoblasts, Flavones, Mice, AMP-Activated Protein Kinases
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