Cited by 78Bioactive Compounds in PlantsExercise and Physiological ResponsesAdipose Tissue and Metabolism

Citrus Tangeretin Improves Skeletal Muscle Mitochondrial Biogenesis via Activating the AMPK-PGC1-α Pathway In Vitro and In Vivo: A Possible Mechanism for Its Beneficial Effect on Physical Performance

Kou, Guangning, Li, Zhenqing, Wu, Chao, Liu, Yang, Hu, Yan, Guo, Liya, Xu, Xiaoyu, Zhou, Zhiqin·2018·Journal of Agricultural and Food Chemistry

Abstract

Mitochondrial biogenesis is a key factor, which influences the function of skeletal muscle. Increasingly, flavonoids are reported to have the potential ability of regulating mitochondrial biogenesis. In this study, we investigated the effects of tangeretin, a polymethoxylated flavonoid isolated from mandarin fruits, on mitochondrial biogenesis and its underlying mechanisms. The tangeretin was obtained from the peel of "Dahongpao" tangerine by macroporous adsorptive resins combined with preparative-high performance liquid chromatography. The activity of mitochondrial biogenesis was explored by using mouse-derived C2C12 myoblasts and Kunming mice. Results showed that the purity of tangeretin obtained was 98.64%, and it could effectively activate mitochondrial biogenesis signaling pathway both at gene and at protein levels in C2C12 myoblasts. Animal experiments showed that tangeretin pretreatment could markedly improve exercise performance (the time of hanging wire and run to fatigue was obviously increased 1.6-fold and 2.1-fold in the high-dose tangeretin group, respectively), and the transmission electron microscopy, Western blotting, and immunohistochemistry further indicated that tangeretin increased mitochondria number and activated mitochondrial biogenesis signaling axis. Our findings suggest that tangeretin enhanced mitochondrial biogenesis via activating the AMPK-PGC1-α pathway, resulting in the improvement of exercise performance, and tangeretin may be a potentially novel mitochondria regulator in foods.

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Crossref · 10.1021/acs.jafc.8b04124

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Authors
Kou, G., Li, Z., Wu, C., Liu, Y., Hu, Y., Guo, L., Xu, X., & Zhou, Z.
Affiliations
Southwest University (CN)
Corresponding author
  • Xu, Xiaoyu
  • Zhou, Zhiqin
Venue
Journal of Agricultural and Food Chemistry
Published
2018-10-28
Volume
66
Issue
45
Pages
11917-11925
ISSN
  • 0021-8561 (ISSN-L)
  • 1520-5118
OpenAlex
W2898547892
Cited by
78
References
36
Citations by year
2026: 9 · 2025: 14 · 2024: 16 · 2023: 7 · 2022: 13 · 2021: 9 · 2020: 9 · 2019: 1
FWCI
4.20
Type
journal article
Language
en
Open access
closed
Locations
  • Journal of Agricultural and Food Chemistry (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Natural Science Foundation of China (31772260)
  • National Risk Assessment Program for Agricultural Products Quality and Safety (GJFP201801502)
  • Chongqing Program for Production of Late Maturing Citrus Fruits and Plums (2018-01-4)
  • Chongqing Graduate Student Research Innovation Project (CYB 18081)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Bioactive Compounds in Plants
  • Health Sciences › Medicine › Rehabilitation › Exercise and Physiological Responses
  • Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
Keywords
tangeretin, citrus flavonoids, mitochondrial biogenesis, skeletal muscle, exercise performance, C2C12 myoblasts, tangerine peel, flavonoid purification, fatigue resistance
MeSH terms
Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha, Animals, Organelle Biogenesis, Cell Differentiation, Citrus, Male, Mitochondria, Physical Conditioning, Animal, Plant Extracts, Muscle, Skeletal, Myoblasts, Flavones, Mice, AMP-Activated Protein Kinases

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