The DOI matches its Crossref record; 54 of the 54 references it cites were found in a registry or an index.
Dextran-Coated Iron Oxide Nanoparticles as Biomimetic Catalysts for Localized and pH-Activated Biofilm Disruption
Open accessAbstract
Biofilms are surface-attached bacterial communities embedded within an extracellular matrix that create localized and protected microenvironments. Acidogenic oral biofilms can demineralize the enamel-apatite on teeth, causing dental caries (tooth decay). Current antimicrobials have low efficacy and do not target the protective matrix and acidic pH within the biofilm. Recently, catalytic nanoparticles were shown to disrupt biofilms but lacked a stabilizing coating required for clinical applications. Here, we report dextran-coated iron oxide nanoparticles termed nanozymes (Dex-NZM) that display strong catalytic (peroxidase-like) activity at acidic pH values, target biofilms with high specificity, and prevent severe caries without impacting surrounding oral tissues in vivo . Nanoparticle formulations were synthesized with dextran coatings (molecular weights from 1.5 to 40 kDa were used), and their catalytic performance and bioactivity were assessed. We found that 10 kDa dextran coating provided maximal catalytic activity, biofilm uptake, and antibiofilm properties. Mechanistic studies indicated that iron oxide cores are the source of catalytic activity, whereas dextran on the nanoparticle surface provided stability without blocking catalysis. Dextran-coating facilitated NZM incorporation into exopolysaccharides (EPS) structure and binding within biofilms, which activated hydrogen peroxide (H 2 O 2 ) for localized bacterial killing and EPS-matrix breakdown. Surprisingly, dextran coating enhanced selectivity toward biofilms while avoiding binding to gingival cells. Furthermore, Dex-NZM/H 2 O 2 treatment significantly reduced the onset and severity of caries lesions (vs control or either Dex-NZM or H 2 O 2 alone) without adverse effects on gingival tissues or oral microbiota diversity in vivo . Therefore, dextran-coated nanozymes have potential as an alternative treatment to control tooth decay and possibly other biofilm-associated diseases.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Flemming, H.-C., & Wingender, J. (2010) · Nature Reviews Microbiology · doi:10.1038/nrmicro2415 · Cited by 10,137
Intrinsic peroxidase-like activity of ferromagnetic nanoparticles
Gao, L., Zhuang, J., Nie, L., Zhang, J., Zhang, Y., Gu, N., Wang, T., Feng, J., Yang, D., Perrett, S., et al. (2007) · Nature Nanotechnology · doi:10.1038/nnano.2007.260 · Cited by 7,483
Self-citationVos, T., Allen, C., Arora, M., Barber, R. M., Bhutta, Z. A., Brown, A., Carter, A., Casey, D. C., Charlson, F. J., Chen, A. Z., et al. (2016) · The Lancet · doi:10.1016/s0140-6736(16)31678-6 · Cited by 7,253
Self-citationNanomaterials with enzyme-like characteristics (nanozymes): next-generation artificial enzymes
Wei, H., & Wang, E. (2013) · Chemical Society Reviews · doi:10.1039/c3cs35486e · Cited by 3,779
Pitts, N. B., Zero, D. T., Marsh, P. D., Ekstrand, K., Weintraub, J. A., Ramos-Gomez, F., Tagami, J., Twetman, S., Tsakos, G., & Ismail, A. (2017) · Nature Reviews Disease Primers · doi:10.1038/nrdp.2017.30 · Cited by 1,823
Targeting microbial biofilms: current and prospective therapeutic strategies
Koo, H., Allan, R. N., Howlin, R. P., Stoodley, P., & Hall-Stoodley, L. (2017) · Nature Reviews Microbiology · doi:10.1038/nrmicro.2017.99 · Cited by 1,800
Self-citationGlobal Burden of Untreated Caries: A Systematic Review and Metaregression
Kassebaum, N. J., Bernabé, E., Dahiya, M., Bhandari, B., Murray, C. J. L., & Marcenes, W. (2015) · Journal of Dental Research · doi:10.1177/0022034515573272 · Cited by 1,679
Recent advances in iron oxide nanocrystal technology for medical imaging☆
COROT, C., ROBERT, P., IDEE, J., & PORT, M. (2006) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2006.09.013 · Cited by 1,465
The Challenge of Treating Biofilm-associated Bacterial Infections
del Pozo, J. L., & Patel, R. (2007) · Clinical Pharmacology & Therapeutics · doi:10.1038/sj.clpt.6100247 · Cited by 671
Tassa, C., Shaw, S. Y., & Weissleder, R. (2011) · Accounts of Chemical Research · doi:10.1021/ar200084x · Cited by 653
Jung, C. W., & Jacobs, P. (1995) · Magnetic Resonance Imaging · doi:10.1016/0730-725x(95)00024-b · Cited by 622
Falsetta, M. L., Klein, M. I., Colonne, P. M., Scott-Anne, K., Gregoire, S., Pai, C.-H., Gonzalez-Begne, M., Watson, G., Krysan, D. J., Bowen, W. H., et al. (2014) · Infection and Immunity · doi:10.1128/iai.00087-14 · Cited by 561
Self-citationHernández-Sierra, J. F., Ruiz, F., Cruz Pena, D. C., Martínez-Gutiérrez, F., Martínez, A. E., de Jesús Pozos Guillén, A., Tapia-Pérez, H., & Martínez Castañón, G. (2008) · Nanomedicine: Nanotechnology, Biology and Medicine · doi:10.1016/j.nano.2008.04.005 · Cited by 557
The Exopolysaccharide Matrix: A Virulence Determinant of Cariogenic Biofilm
Koo, H., Falsetta, M. L., & Klein, M. I. (2013) · Journal of Dental Research · doi:10.1177/0022034513504218 · Cited by 523
Self-citationXiao, J., Klein, M. I., Falsetta, M. L., Lu, B., Delahunty, C. M., Yates, J. R., Heydorn, A., & Koo, H. (2012) · PLoS Pathogens · doi:10.1371/journal.ppat.1002623 · Cited by 514
Self-citationKoo, H., Xiao, J., Klein, M. I., & Jeon, J. G. (2010) · Journal of Bacteriology · doi:10.1128/jb.01649-09 · Cited by 508
Self-citationEnzyme Mimicry for Combating Bacteria and Biofilms
Chen, Z., Wang, Z., Ren, J., & Qu, X. (2018) · Accounts of Chemical Research · doi:10.1021/acs.accounts.8b00011 · Cited by 499
Nanomaterials in preventive dentistry
Hannig, M., & Hannig, C. (2010) · Nature Nanotechnology · doi:10.1038/nnano.2010.83 · Cited by 414
The complex oral microflora of high‐risk individuals and groups and its role in the caries process
Beighton, D. (2005) · Community Dentistry and Oral Epidemiology · doi:10.1111/j.1600-0528.2005.00232.x · Cited by 360
Horev, B., Klein, M. I., Hwang, G., Li, Y., Kim, D., Koo, H., & Benoit, D. S. W. (2015) · ACS Nano · doi:10.1021/nn507170s · Cited by 340
Self-citationGao, L., Liu, Y., Kim, D., Li, Y., Hwang, G., Naha, P. C., Cormode, D. P., & Koo, H. (2016) · Biomaterials · doi:10.1016/j.biomaterials.2016.05.051 · Cited by 324
Self-citationBesinis, A., De Peralta, T., Tredwin, C. J., & Handy, R. D. (2015) · ACS Nano · doi:10.1021/nn505015e · Cited by 273
Synthesis of extracellular dextran by cariogenic bacteria and its presence in human dental plaque
Gibbons, R. J., & Banghart, S. B. (1967) · Archives of Oral Biology · doi:10.1016/0003-9969(67)90137-9 · Cited by 267
Gao, L., Giglio, K. M., Nelson, J. L., Sondermann, H., & Travis, A. J. (2014) · Nanoscale · doi:10.1039/c3nr05422e · Cited by 265
Self-citationvan Schooneveld, M. M., Vucic, E., Koole, R., Zhou, Y., Stocks, J., Cormode, D. P., Tang, C. Y., Gordon, R. E., Nicolay, K., Meijerink, A., et al. (2008) · Nano Letters · doi:10.1021/nl801596a · Cited by 256
Self-citationdos Santos, V. E., Filho, A. V., Ribeiro Targino, A. G., Pelagio Flores, M. A., Galembeck, A., Caldas, A. F., & Rosenblatt, A. (2014) · Journal of Dentistry · doi:10.1016/j.jdent.2014.05.017 · Cited by 229
Decomposing phenol by the hidden talent of ferromagnetic nanoparticles
Zhang, J., Zhuang, J., Gao, L., Zhang, Y., Gu, N., Feng, J., Yang, D., Zhu, J., & Yan, X. (2008) · Chemosphere · doi:10.1016/j.chemosphere.2008.05.050 · Cited by 228
Self-citationSafety and technique of ferumoxytol administration for MRI
Vasanawala, S. S., Nguyen, K.-L., Hope, M. D., Bridges, M. D., Hope, T. A., Reeder, S. B., & Bashir, M. R. (2016) · Magnetic Resonance in Medicine · doi:10.1002/mrm.26151 · Cited by 207
Targeted iron oxide nanoparticles for the enhancement of radiation therapy
Hauser, A. K., Mitov, M. I., Daley, E. F., McGarry, R. C., Anderson, K. W., & Hilt, J. Z. (2016) · Biomaterials · doi:10.1016/j.biomaterials.2016.07.032 · Cited by 204
Emerging Biomedical Applications of Enzyme-Like Catalytic Nanomaterials
Cormode, D. P., Gao, L., & Koo, H. (2018) · Trends in Biotechnology · doi:10.1016/j.tibtech.2017.09.006 · Cited by 199
Self-citationNanoparticles and the control of oral infections
Allaker, R. P., & Memarzadeh, K. (2014) · International Journal of Antimicrobial Agents · doi:10.1016/j.ijantimicag.2013.11.002 · Cited by 190
Klein, M. I., Duarte, S., Xiao, J., Mitra, S., Foster, T. H., & Koo, H. (2009) · Applied and Environmental Microbiology · doi:10.1128/aem.01299-08 · Cited by 154
Self-citationGarg, B., Bisht, T., & Ling, Y. (2015) · Molecules · doi:10.3390/molecules200814155 · Cited by 150
Kim, D., Liu, Y., Benhamou, R. I., Sánchez, H., Simón‐Soro, Á., Li, Y. H., Hwang, G., Fridman, M., Andes, D. R., & Koo, H. (2018) · The ISME Journal · doi:10.1038/s41396-018-0113-1 · Cited by 144
Self-citationNaha, P. C., Zaki, A. A., Hecht, E. M., Chorny, M., Chhour, P., Blankemeyer, E. R., Yates, D. M., Witschey, W. R. T., Litt, H. I., Tsourkas, A., et al. (2014) · Journal of Materials Chemistry B · doi:10.1039/c4tb01159g · Cited by 133
Self-citationGuggenheim, B., Gmür, R., Galicia, J. C., Stathopoulou, P. G., Benakanakere, M. R., Meier, A., Thurnheer, T., & Kinane, D. F. (2009) · BMC Microbiology · doi:10.1186/1471-2180-9-280 · Cited by 122
Nanotechnology and Its Role in Caries Therapy
Hannig, M., & Hannig, C. (2012) · Advances in Dental Research · doi:10.1177/0022034512450446 · Cited by 121
Sensing H2O2 with layer-by-layer assembled Fe3O4–PDDA nanocomposite film
Zhang, L., Zhai, Y., Gao, N., Wen, D., & Dong, S. (2008) · Electrochemistry Communications · doi:10.1016/j.elecom.2008.05.022 · Cited by 112
Unterweger, H., Janko, C., Schwarz, M. A., Dézsi, L., Urbanics, R., Matuszak, J., Őrfi, E., Fülöp, T., Bäuerle, T., Szebeni, J., et al. (2017) · International Journal of Nanomedicine · doi:10.2147/ijn.s138108 · Cited by 108
Fujimaki, M., Koo, H., Smith, A. M. V., Kopec, L. K., Scott-Anne, K., Cury, J. A., & Bowen, W. H. (2004) · Carbohydrate Research · doi:10.1016/j.carres.2004.05.031 · Cited by 103
Self-citationNanosilver coated orthodontic brackets:in vivoantibacterial properties and ion release
Metin-Gürsoy, G., Taner, L., & Akca, G. (2016) · European Journal of Orthodontics · doi:10.1093/ejo/cjv097 · Cited by 99
Naha, P. C., Lau, K. C., Hsu, J. C., Hajfathalian, M., Mian, S., Chhour, P., Uppuluri, L., McDonald, E. S., Maidment, A. D. A., & Cormode, D. P. (2016) · Nanoscale · doi:10.1039/c6nr02618d · Cited by 95
Self-citationRodent model in caries research
Bowen, W. H. (2012) · Odontology · doi:10.1007/s10266-012-0091-0 · Cited by 78
Naha, P. C., Chhour, P., & Cormode, D. P. (2015) · Toxicology in Vitro · doi:10.1016/j.tiv.2015.05.022 · Cited by 70
Self-citationProduction and purification of the multifunctional enzyme horseradish peroxidase
Oliver Spadiut, A., & Herwig, C. (2013) · Pharmaceutical Bioprocessing · doi:10.4155/pbp.13.23 · Cited by 59
Cormode, D. P., Sánchez-Gaytán, B. L., Mieszawska, A. J., Fayad, Z. A., & Mulder, W. J. M. (2013) · NMR in Biomedicine · doi:10.1002/nbm.2909 · Cited by 56
Self-citationFacile Method for the Site‐Specific, Covalent Attachment of Full‐Length IgG onto Nanoparticles
Hui, J. Z., Zaki, A. A., Cheng, Z., Popik, V. V., Zhang, H., Prak, E. T. L., & Tsourkas, A. (2014) · Small · doi:10.1002/smll.201303629 · Cited by 54
Hwang, G., Koltisko, B., Jin, X., & Koo, H. (2017) · ACS Applied Materials & Interfaces · doi:10.1021/acsami.7b11558 · Cited by 52
Self-citationIonescu, A. C., Brambilla, E., Travan, A., Marsich, E., Donati, I., Gobbi, P., Turco, G., Lenarda, R. D., Cadenaro, M., Paoletti, S., et al. (2015) · Journal of Dentistry · doi:10.1016/j.jdent.2015.10.006 · Cited by 35
de Sousa, F. F. O., Ferraz, C. L. H., Rodrigues, L. K. A., Nojosa, J. S., & Yamauti, M. (2014) · Current Drug Delivery · doi:10.2174/156720181106141202115157 · Cited by 26
He, Y., Peterson, B. W., Ren, Y., van der Mei, H. C., & Busscher, H. J. (2013) · Clinical Oral Investigations · doi:10.1007/s00784-013-1097-x · Cited by 21
Do Catalytic Nanoparticles Offer an Improved Therapeutic Strategy to Combat Dental biofilms?
Gao, L., & Koo, H. (2017) · Nanomedicine · doi:10.2217/nnm-2016-0400 · Cited by 20
Self-citationvan Tilborg, G. A. F., Cormode, D. P., Jarzyna, P. A., van der Toorn, A., van der Pol, S. M. A., van Bloois, L., Fayad, Z. A., Storm, G., Mulder, W. J. M., de Vries, H. E., et al. (2012) · Bioconjugate Chemistry · doi:10.1021/bc200543k · Cited by 13
Self-citationFattahian, Y., Riahi‐Madvar, A., Mirzaee, R., Asadikaram, G., & Rahbar, M. R. (2017) · Computational Biology and Chemistry · doi:10.1016/j.compbiolchem.2017.07.003 · Cited by 6
Cited by
Yang, B., Chen, Y., & Shi, J. (2019) · Advanced Materials · doi:10.1002/adma.201901778 · Cited by 601
Bioadhesion in the oral cavity and approaches for biofilm management by surface modifications
Sterzenbach, T., Helbig, R., Hannig, C., & Hannig, M. (2020) · Clinical Oral Investigations · doi:10.1007/s00784-020-03646-1 · Cited by 212
Cariogenic Biofilm: Pathology-Related Phenotypes and Targeted Therapy
Chen, X., Daliri, E. B.-M., Tyagi, A., & Oh, D.-H. (2021) · Microorganisms · doi:10.3390/microorganisms9061311 · Cited by 50
Metadata
- Authors
- Naha, P. C., Liu, Y., Hwang, G., Huang, Y., Gubara, S., Jonnakuti, V., Simon-Soro, A., Kim, D., Gao, L., Koo, H., & Cormode, D. P.
- Venue
- ACS Nano
- Publisher
- American Chemical Society (ACS)
- Published
- 2019-01-14
- Volume
- 13
- Issue
- 5
- Pages
- 4960-4971
- ISSN
- 1936-0851 (ISSN-L)
- 1936-086X
- OpenAlex
- W2909347085
- Cited by
- 367
- References
- 54
- FWCI
- 13.98
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
