Assessment of Anabaena sp. Strain PCC 7120 as a Heterologous Expression Host for Cyanobacterial Natural Products: Production of Lyngbyatoxin A
Abstract
Cyanobacteria are well-known producers of natural products of highly varied structure and biological properties. However, the long doubling times, difficulty in establishing genetic methods for marine cyanobacteria, and low compound titers have hindered research into the biosynthesis of their secondary metabolites. While a few attempts to heterologously express cyanobacterial natural products have occurred, the results have been of varied success. Here, we report the first steps in developing the model freshwater cyanobacterium Anabaena sp. strain PCC 7120 (Anabaena 7120) as a general heterologous expression host for cyanobacterial secondary metabolites. We show that Anabaena 7120 can heterologously synthesize lyngbyatoxin A in yields comparable to those of the native producer, Moorea producens, and detail the design and use of replicative plasmids for compound production. We also demonstrate that Anabaena 7120 recognizes promoters from various biosynthetic gene clusters from both free-living and obligate symbiotic marine cyanobacteria. Through simple genetic manipulations, the titer of lyngbyatoxin A can be improved up to 13-fold. The development of Anabaena 7120 as a general heterologous expression host enables investigation of interesting cyanobacterial biosynthetic reactions and genetic engineering of their biosynthetic pathways.
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Videau et al. expressed lyngbyatoxin A biosynthetic genes in Anabaena sp. PCC 7120 and demonstrated production of lyngbyatoxin A at levels comparable to those reported for the native producer, Moorea producens; titres could be improved through genetic and promoter-level optimisation.
“Videau et al. expressed the lyngbyatoxin A biosynthetic genes in Anabaena sp. PCC 7120 and demonstrated production of lyngbyatoxin A at levels comparable to those reported for the native producer, Moorea producens; importantly, the study also showed that titres could be improved through genetic and promoter-level optimisation [182].”
From Cyanobacteria as a Photosynthetic Chassis for Metabolic Pathway Engineering with Heterologous Gene Expression (2026) · cites this paper as [182]
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Functions, Structures, and Engineering of the Teleocidin Biosynthetic Enzymes
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“…A biosynthetic genes in Anabaena sp. PCC 7120 and demonstrated production of lyngbyatoxin A at levels comparable to those reported for the native producer, Moorea producens ; importantly, the study also showed that titres could be improved through genetic and promoter-level optimisation [182].”
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- Authors
- Videau, P., Wells, K. N., Singh, A. J., Gerwick, W. H., & Philmus, B.
- Affiliations
- Oregon State University (US)
- Scripps Institution of Oceanography (US)
- Corresponding author
- Philmus, Benjamin
- Venue
- ACS Synthetic Biology
- Publisher
- American Chemical Society (ACS)
- Published
- 2016-06-01
- Volume
- 5
- Issue
- 9
- Pages
- 978-988
- ISSN
- 2161-5063 (ISSN-L)
- OpenAlex
- W2364077778
- Cited by
- 66
- References
- 57
- Citations by year
- 2026: 9 · 2025: 6 · 2024: 1 · 2023: 7 · 2022: 5 · 2021: 5 · 2020: 8 · 2019: 10 · 2018: 10 · 2017: 5
- FWCI
- 6.74
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- ACS Synthetic Biology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- College of Pharmacy, Oregon State University
- Medical Research Foundation, Oregon Health and Science University (1415)
- Topics
- Health Sciences › Medicine › Pharmacology › Microbial Natural Products and Biosynthesis
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant biochemistry and biosynthesis
- Keywords
- Anabaena sp. PCC 7120, heterologous expression, cyanobacterial natural products, lyngbyatoxin A, cyanobacterial secondary metabolites, biosynthetic gene clusters, promoter identification
- MeSH terms
- Cyanobacteria, Bacterial Proteins, Biological Products, Genes, Bacterial, Lyngbya Toxins, Plasmids, Promoter Regions, Genetic, Anabaena, Biosynthetic Pathways
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