Cited by 66Microbial Natural Products and BiosynthesisMicrobial Metabolic Engineering and BioproductionPlant biochemistry and biosynthesis

Assessment of Anabaena sp. Strain PCC 7120 as a Heterologous Expression Host for Cyanobacterial Natural Products: Production of Lyngbyatoxin A

Videau, Patrick, Wells, Kaitlyn N., Singh, Arun J., Gerwick, William H., Philmus, Benjamin·2016·ACS Synthetic Biology

Abstract

Cyanobacteria are well-known producers of natural products of highly varied structure and biological properties. However, the long doubling times, difficulty in establishing genetic methods for marine cyanobacteria, and low compound titers have hindered research into the biosynthesis of their secondary metabolites. While a few attempts to heterologously express cyanobacterial natural products have occurred, the results have been of varied success. Here, we report the first steps in developing the model freshwater cyanobacterium Anabaena sp. strain PCC 7120 (Anabaena 7120) as a general heterologous expression host for cyanobacterial secondary metabolites. We show that Anabaena 7120 can heterologously synthesize lyngbyatoxin A in yields comparable to those of the native producer, Moorea producens, and detail the design and use of replicative plasmids for compound production. We also demonstrate that Anabaena 7120 recognizes promoters from various biosynthetic gene clusters from both free-living and obligate symbiotic marine cyanobacteria. Through simple genetic manipulations, the titer of lyngbyatoxin A can be improved up to 13-fold. The development of Anabaena 7120 as a general heterologous expression host enables investigation of interesting cyanobacterial biosynthetic reactions and genetic engineering of their biosynthetic pathways.

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Crossref · 10.1021/acssynbio.6b00038

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  • Videau et al. expressed lyngbyatoxin A biosynthetic genes in Anabaena sp. PCC 7120 and demonstrated production of lyngbyatoxin A at levels comparable to those reported for the native producer, Moorea producens; titres could be improved through genetic and promoter-level optimisation.

    “Videau et al. expressed the lyngbyatoxin A biosynthetic genes in Anabaena sp. PCC 7120 and demonstrated production of lyngbyatoxin A at levels comparable to those reported for the native producer, Moorea producens; importantly, the study also showed that titres could be improved through genetic and promoter-level optimisation [182].”

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Authors
Videau, P., Wells, K. N., Singh, A. J., Gerwick, W. H., & Philmus, B.
Affiliations
  • Oregon State University (US)
  • Scripps Institution of Oceanography (US)
Corresponding author
Philmus, Benjamin
Venue
ACS Synthetic Biology
Published
2016-06-01
Volume
5
Issue
9
Pages
978-988
ISSN
2161-5063 (ISSN-L)
OpenAlex
W2364077778
Cited by
66
References
57
Citations by year
2026: 9 · 2025: 6 · 2024: 1 · 2023: 7 · 2022: 5 · 2021: 5 · 2020: 8 · 2019: 10 · 2018: 10 · 2017: 5
FWCI
6.74
Type
journal article
Language
en
Open access
closed
Locations
  • ACS Synthetic Biology (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • College of Pharmacy, Oregon State University
  • Medical Research Foundation, Oregon Health and Science University (1415)
Topics
  • Health Sciences › Medicine › Pharmacology › Microbial Natural Products and Biosynthesis
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant biochemistry and biosynthesis
Keywords
Anabaena sp. PCC 7120, heterologous expression, cyanobacterial natural products, lyngbyatoxin A, cyanobacterial secondary metabolites, biosynthetic gene clusters, promoter identification
MeSH terms
Cyanobacteria, Bacterial Proteins, Biological Products, Genes, Bacterial, Lyngbya Toxins, Plasmids, Promoter Regions, Genetic, Anabaena, Biosynthetic Pathways

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