Cited by 33Microbial Natural Products and BiosynthesisMicrobial Metabolic Engineering and BioproductionPlant biochemistry and biosynthesis

Heterologous production of cyanobacterial compounds

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Dhakal, Dipesh, Chen, Manyun, Luesch, Hendrik, Ding, Yousong·2021·Journal of Industrial Microbiology and Biotechnology

Abstract

Cyanobacteria produce a plethora of compounds with unique chemical structures and diverse biological activities. Importantly, the increasing availability of cyanobacterial genome sequences and the rapid development of bioinformatics tools have unraveled the tremendous potential of cyanobacteria in producing new natural products. However, the discovery of these compounds based on cyanobacterial genomes has progressed slowly as the majority of their corresponding biosynthetic gene clusters (BGCs) are silent. In addition, cyanobacterial strains are often slow-growing, difficult for genetic engineering, or cannot be cultivated yet, limiting the use of host genetic engineering approaches for discovery. On the other hand, genetically tractable hosts such as Escherichia coli, Actinobacteria, and yeast have been developed for the heterologous expression of cyanobacterial BGCs. More recently, there have been increased interests in developing model cyanobacterial strains as heterologous production platforms. Herein, we present recent advances in the heterologous production of cyanobacterial compounds in both cyanobacterial and noncyanobacterial hosts. Emerging strategies for BGC assembly, host engineering, and optimization of BGC expression are included for fostering the broader applications of synthetic biology tools in the discovery of new cyanobacterial natural products.

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Authors
Dhakal, D., Chen, M., Luesch, H., & Ding, Y.
Affiliations
  • University of Florida (US)
  • Department of Medicinal Chemistry, Center for Natural Products, Drug Discovery and Development, University of Florida , Gainesville, FL 31610, USA
Corresponding author
  • Luesch, Hendrik
  • Ding, Yousong
Venue
Journal of Industrial Microbiology and Biotechnology
Published
2021-01-25
Volume
48
Issue
3-4
ISSN
  • 1367-5435 (ISSN-L)
  • 1476-5535
OpenAlex
W3123060788
Cited by
33
References
171
Citations by year
2026: 7 · 2025: 8 · 2024: 2 · 2023: 5 · 2022: 8 · 2021: 2 · 2015: 1
FWCI
0.54
Type
journal article
Language
en
Open access
Locations
  • Journal of Industrial Microbiology & Biotechnology (publishedVersion, other-oa)
  • PubMed Central (publishedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health (R01CA172310, R35GM128742)
  • University of Florida
Topics
  • Health Sciences › Medicine › Pharmacology › Microbial Natural Products and Biosynthesis
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant biochemistry and biosynthesis
Keywords
cyanobacterial natural products, heterologous expression, biosynthetic gene clusters, host engineering, synthetic biology, Escherichia coli, yeast, actinobacteria
MeSH terms
Cyanobacteria, Animals, Biological Products, Multigene Family, Genetic Engineering, Humans

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