Heterologous production of cyanobacterial compounds
Open accessAbstract
Cyanobacteria produce a plethora of compounds with unique chemical structures and diverse biological activities. Importantly, the increasing availability of cyanobacterial genome sequences and the rapid development of bioinformatics tools have unraveled the tremendous potential of cyanobacteria in producing new natural products. However, the discovery of these compounds based on cyanobacterial genomes has progressed slowly as the majority of their corresponding biosynthetic gene clusters (BGCs) are silent. In addition, cyanobacterial strains are often slow-growing, difficult for genetic engineering, or cannot be cultivated yet, limiting the use of host genetic engineering approaches for discovery. On the other hand, genetically tractable hosts such as Escherichia coli, Actinobacteria, and yeast have been developed for the heterologous expression of cyanobacterial BGCs. More recently, there have been increased interests in developing model cyanobacterial strains as heterologous production platforms. Herein, we present recent advances in the heterologous production of cyanobacterial compounds in both cyanobacterial and noncyanobacterial hosts. Emerging strategies for BGC assembly, host engineering, and optimization of BGC expression are included for fostering the broader applications of synthetic biology tools in the discovery of new cyanobacterial natural products.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
These factors can collectively reduce pathway efficiency and restrict product yields.
“These factors can collectively reduce pathway efficiency and restrict product yields [33].”
From Cyanobacteria as a Photosynthetic Chassis for Metabolic Pathway Engineering with Heterologous Gene Expression (2026) · cites this paper as [33]
These features have contributed to growing interest in using Anabaena sp. PCC 7120 as a chassis organism for synthetic biology and metabolic pathway engineering research.
“These features have contributed to growing interest in using Anabaena sp. PCC 7120 as a chassis organism for synthetic biology and metabolic pathway engineering research [33,75].”
From Cyanobacteria as a Photosynthetic Chassis for Metabolic Pathway Engineering with Heterologous Gene Expression (2026) · cites this paper as [33]
These developments have significantly advanced the capacity to design and implement engineered metabolic pathways in cyanobacterial hosts.
“These developments have significantly advanced the capacity to design and implement engineered metabolic pathways in cyanobacterial hosts [33].”
From Cyanobacteria as a Photosynthetic Chassis for Metabolic Pathway Engineering with Heterologous Gene Expression (2026) · cites this paper as [33]
Construction of heterologous metabolic pathways in cyanobacteria generally involves coordinated expression of multiple genes encoding enzymes responsible for sequential biochemical reactions.
“The construction of heterologous metabolic pathways in cyanobacteria generally involves the coordinated expression of multiple genes that encode enzymes responsible for sequential biochemical reactions [33].”
From Cyanobacteria as a Photosynthetic Chassis for Metabolic Pathway Engineering with Heterologous Gene Expression (2026) · cites this paper as [33]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products.
Datsenko, K., & Wanner, B. (2000) · Proceedings of the National Academy of Sciences of the United States of America · doi:10.1073/pnas.120163297 · Cited by 15,902 · see citing works
Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression
Qi, L. S., Larson, M. H., Gilbert, L. A., Doudna, J. A., Weissman, J. S., Arkin, A. P., & Lim, W. A. (2013) · Cell · doi:10.1016/j.cell.2013.02.022 · Cited by 5,016 · see citing works
Wang, M., Carver, J. J., Phelan, V. V., Sanchez, L. M., Garg, N., Peng, Y., Nguyen, D. D., Watrous, J., Kapono, C. A., Luzzatto-Knaan, T., et al. (2016) · Nature Biotechnology · doi:10.1038/nbt.3597 · Cited by 4,629 · see citing works
antiSMASH 5.0: updates to the secondary metabolite genome mining pipeline
Blin, K., Shaw, S., Steinke, K., Villebro, R., Ziemert, N., Lee, S. Y., Medema, M. H., & Weber, T. (2019) · Nucleic Acids Research · doi:10.1093/nar/gkz310 · Cited by 2,915 · see citing works
Recombinant protein expression in Escherichia coli: advances and challenges
Rosano, G. L., & Ceccarelli, E. A. (2014) · Frontiers in Microbiology · doi:10.3389/fmicb.2014.00172 · Cited by 2,556 · see citing works
Compilation and analysis ofEscherichia colipromoter DNA sequences
Hawley, D. K., & McClure, W. R. (1983) · Nucleic Acids Research · doi:10.1093/nar/11.8.2237 · Cited by 2,217 · see citing works
Automated design of synthetic ribosome binding sites to control protein expression
Salis, H. M., Mirsky, E. A., & Voigt, C. A. (2009) · Nature Biotechnology · doi:10.1038/nbt.1568 · Cited by 1,844 · see citing works
Cyanobacteria secondary metabolites—the cyanotoxins
Carmichael, W. W. (1992) · Journal of Applied Bacteriology · doi:10.1111/j.1365-2672.1992.tb01858.x · Cited by 1,457 · see citing works
A computational framework to explore large-scale biosynthetic diversity
Navarro-Muñoz, J. C., Mojica, N. S., Mullowney, M. W., Kautsar, S. A., Tryon, J. H., Parkinson, E. I., Santos, E. L. C. D. L., Yeong, M., Cruz‐Morales, P., Abubucker, S., et al. (2019) · Nature Chemical Biology · doi:10.1038/s41589-019-0400-9 · Cited by 1,032 · see citing works
Biology and Applications of CRISPR Systems: Harnessing Nature’s Toolbox for Genome Engineering
Wright, A. V., Nuñez, J. K., & Doudna, J. A. (2016) · Cell · doi:10.1016/j.cell.2015.12.035 · Cited by 1,001 · see citing works
A new enzyme superfamily — the phosphopantetheinyl transferases
Lambalot, R. H., Gehring, A. M., Flugel, R. S., Zuber, P., LaCelle, M. G., Marahiel, M. A., Reid, R. C., Khosla, C., & Walsh, C. T. (1996) · Chemistry & Biology · doi:10.1016/s1074-5521(96)90181-7 · Cited by 845 · see citing works
Biosynthesis of Complex Polyketides in a Metabolically Engineered Strain of E. coli
Pfeifer, B. A., Admiraal, S. J., Gramajo, H. C., Cane, D. E., & Khosla, C. (2001) · Science · doi:10.1126/science.1058092 · Cited by 751 · see citing works
Molecular Mechanisms Underlying Nonribosomal Peptide Synthesis: Approaches to New Antibiotics
Sieber, S. A., & Marahiel, M. A. (2005) · Chemical Reviews · doi:10.1021/cr0301191 · Cited by 594 · see citing works
Schmidt, E. W., Nelson, J. T., Rasko, D. A., Sudek, S., Eisen, J. A., Haygood, M. G., & Ravel, J. (2005) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0501424102 · Cited by 567 · see citing works
MIBiG 2.0: a repository for biosynthetic gene clusters of known function
Kautsar, S. A., Blin, K., Shaw, S., Navarro-Muñoz, J. C., Terlouw, B. R., van der Hooft, J. J. J., van Santen, J. A., Tracanna, V., Duran, H. G. S., Andreu, V. P., et al. (2019) · Nucleic Acids Research · doi:10.1093/nar/gkz882 · Cited by 562 · see citing works
Gomez‐Escribano, J. P., & Bibb, M. J. (2010) · Microbial Biotechnology · doi:10.1111/j.1751-7915.2010.00219.x · Cited by 556 · see citing works
Engineered riboregulators enable post-transcriptional control of gene expression
Isaacs, F. J., Dwyer, D. J., Ding, C., Pervouchine, D. D., Cantor, C. R., & Collins, J. J. (2004) · Nature Biotechnology · doi:10.1038/nbt986 · Cited by 547 · see citing works
Genome-minimized Streptomyces host for the heterologous expression of secondary metabolism
Komatsu, M., Uchiyama, T., Ōmura, S., Cane, D. E., & Ikeda, H. (2010) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0914833107 · Cited by 502 · see citing works
A new genome-mining tool redefines the lasso peptide biosynthetic landscape
Tietz, J. I., Schwalen, C. J., Patel, P. S., Maxson, T., Saint‐Vincent, P. M. B., Tai, H.-C., Zakai, U. I., & Mitchell, D. A. (2017) · Nature Chemical Biology · doi:10.1038/nchembio.2319 · Cited by 490 · see citing works
Synthetic biology to access and expand nature's chemical diversity
Smanski, M. J., Zhou, H. L., Claesen, J., Shen, B., Fischbach, M. A., & Voigt, C. A. (2016) · Nature Reviews Microbiology · doi:10.1038/nrmicro.2015.24 · Cited by 482 · see citing works
Borujeni, A. E., Channarasappa, A. S., & Salis, H. M. (2013) · Nucleic Acids Research · doi:10.1093/nar/gkt1139 · Cited by 445 · see citing works
The Natural Products Atlas: An Open Access Knowledge Base for Microbial Natural Products Discovery
van Santen, J. A., Jacob, G., Singh, A. L., Aniebok, V., Balunas, M. J., Bunsko, D., Neto, F. C., Castaño-Espriu, L., Chang, C., Clark, T. N., et al. (2019) · ACS Central Science · doi:10.1021/acscentsci.9b00806 · Cited by 421 · see citing works
Kaur, J., Kumar, A., & Kaur, J. (2017) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2017.08.080 · Cited by 412 · see citing works
Potential of industrial biotechnology with cyanobacteria and eukaryotic microalgae
Wijffels, R. H., Kruse, O., & Hellingwerf, K. J. (2013) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2013.04.004 · Cited by 404 · see citing works
The type I fatty acid and polyketide synthases: a tale of two megasynthases
Smith, S., & Tsai, S. (2007) · Natural Product Reports · doi:10.1039/b603600g · Cited by 397 · see citing works
CyanoMetDB, a comprehensive public database of secondary metabolites from cyanobacteria
Jones, M. R., Pinto, E., Torres, M. D. A., Dörr, F., Mazur‐Marzec, H., Szubert, K., Tartaglione, L., Dell’Aversano, C., Miles, C. O., Beach, D. G., et al. (2021) · Water Research · doi:10.1016/j.watres.2021.117017 · Cited by 378 · see citing works
Beld, J., Sonnenschein, E. C., Vickery, C. R., Noel, J. P., & Burkart, M. D. (2013) · Natural Product Reports · doi:10.1039/c3np70054b · Cited by 360 · see citing works
Kuehnbaum, N. L., & Britz‐McKibbin, P. (2013) · Chemical Reviews · doi:10.1021/cr300484s · Cited by 359 · see citing works
Huang, H.-H., Camsund, D., Lindblad, P. C., & Heidorn, T. (2010) · Nucleic Acids Research · doi:10.1093/nar/gkq164 · Cited by 351 · see citing works
Recombinant pharmaceuticals from microbial cells: a 2015 update
Sánchez‐García, L., Martín, L., Mangues, R., Ferrer‐Miralles, N., Vázquez, E., & Villaverde, A. (2016) · Microbial Cell Factories · doi:10.1186/s12934-016-0437-3 · Cited by 341 · see citing works
Promoter engineering: Recent advances in controlling transcription at the most fundamental level
Blazeck, J., & Alper, H. S. (2012) · Biotechnology Journal · doi:10.1002/biot.201200120 · Cited by 331 · see citing works
Natural Products from Cyanobacteria: Focus on Beneficial Activities
Demay, J., Bernard, C., Reinhardt, A., & Marie, B. (2019) · Marine Drugs · doi:10.3390/md17060320 · Cited by 331 · see citing works
Natural Product Biosynthetic Diversity and Comparative Genomics of the Cyanobacteria
Dittmann, E., Gugger, M. F., Sivonen, K., & Fewer, D. P. (2015) · Trends in Microbiology · doi:10.1016/j.tim.2015.07.008 · Cited by 326 · see citing works
Endolithic Cyanobacteria in Halite Rocks from the Hyperarid Core of the Atacama Desert
Wierzchoś, J., Ascaso, C., & McKay, C. P. (2006) · Astrobiology · doi:10.1089/ast.2006.6.415 · Cited by 321 · see citing works
Cpf1 Is a Versatile Tool for CRISPR Genome Editing Across Diverse Species of Cyanobacteria
Ungerer, J., & Pakrasi, H. B. (2016) · Scientific Reports · doi:10.1038/srep39681 · Cited by 308 · see citing works
Synthetic biology of cyanobacteria: unique challenges and opportunities
Berla, B. M., Saha, R., Immethun, C. M., Maranas, C. D., Moon, T. S., & Pakrasi, H. B. (2013) · Frontiers in Microbiology · doi:10.3389/fmicb.2013.00246 · Cited by 305 · see citing works
Ortega, M. A., & van der Donk, W. A. (2016) · Cell chemical biology · doi:10.1016/j.chembiol.2015.11.012 · Cited by 295 · see citing works
Mass spectrometry searches using MASST
Wang, M., Jarmusch, A. K., Vargas, F., Aksenov, A. A., Gauglitz, J. M., Weldon, K., Petras, D., da Silva, R., Quinn, R., Melnik, A. V., et al. (2020) · Nature Biotechnology · doi:10.1038/s41587-019-0375-9 · Cited by 281 · see citing works
A global assembly line for cyanobactins
Donia, M. S., Ravel, J., & Schmidt, E. W. (2008) · Nature Chemical Biology · doi:10.1038/nchembio.84 · Cited by 279 · see citing works
Zeng, X. L., Zhang, P., He, W., Chu, Q., Chen, S., Tao, L., Wang, Y., Tan, Y. C., Gao, D., Wang, B., et al. (2017) · Nucleic Acids Research · doi:10.1093/nar/gkx1026 · Cited by 273 · see citing works
Cyanobacterial biofuel production
Machado, I. M. P., & Atsumi, S. (2012) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2012.03.005 · Cited by 271 · see citing works
Genomes to natural products PRediction Informatics for Secondary Metabolomes (PRISM)
Skinnider, M. A., Dejong, C. A., Rees, P. N., Johnston, C. W., Li, H., Webster, A., Wyatt, M. A., & Magarvey, N. A. (2015) · Nucleic Acids Research · doi:10.1093/nar/gkv1012 · Cited by 268 · see citing works
Natural combinatorial peptide libraries in cyanobacterial symbionts of marine ascidians
Donia, M. S., Hathaway, B. J., Sudek, S., Haygood, M. G., Rosovitz, M. J., Ravel, J., & Schmidt, E. W. (2006) · Nature Chemical Biology · doi:10.1038/nchembio829 · Cited by 261 · see citing works
Heterologous expression of bacterial natural product biosynthetic pathways
Huo, L., Hug, J. J., Fu, C., Bian, X., Zhang, Y., & Müller, R. (2019) · Natural Product Reports · doi:10.1039/c8np00091c · Cited by 248 · see citing works
Wendt, K. E., Ungerer, J., Cobb, R. E., Zhao, H., & Pakrasi, H. B. (2016) · Microbial Cell Factories · doi:10.1186/s12934-016-0514-7 · Cited by 232 · see citing works
Total biosynthesis of antitumor nonribosomal peptides in Escherichia coli
Watanabe, K., Hotta, K., Praseuth, A. P., Koketsu, K., Migita, A., Boddy, C. N. C., Wang, C. C. C., Oguri, H., & Oikawa, H. (2006) · Nature Chemical Biology · doi:10.1038/nchembio803 · Cited by 223 · see citing works
Myronovskyi, M., Rosenkränzer, B., Nadmid, S., Pujić, P., Normand, P., & Luzhetskyy, A. N. (2018) · Metabolic Engineering · doi:10.1016/j.ymben.2018.09.004 · Cited by 222 · see citing works
New applications of synthetic biology tools for cyanobacterial metabolic engineering
Santos-Merino, M., Singh, A. K., & Ducat, D. C. (2019) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2019.00033 · Cited by 222 · see citing works
Shi, S., Liang, Y., Zhang, M. M., Ang, E. L., & Zhao, H. (2015) · Metabolic Engineering · doi:10.1016/j.ymben.2015.10.011 · Cited by 219 · see citing works
CRISPR/Cas9 advances engineering of microbial cell factories
Jakočiūnas, T., Jensen, M. K., & Keasling, J. D. (2015) · Metabolic Engineering · doi:10.1016/j.ymben.2015.12.003 · Cited by 218 · see citing works
RiPP antibiotics: biosynthesis and engineering potential
Hudson, G. A., & Mitchell, D. A. (2018) · Current Opinion in Microbiology · doi:10.1016/j.mib.2018.02.010 · Cited by 218 · see citing works
Transcription termination and anti‐termination in E. coli
Nudler, E., & Gottesman, M. E. (2002) · Genes to Cells · doi:10.1046/j.1365-2443.2002.00563.x · Cited by 212 · see citing works
Curran, K. A., Karim, A. S., Gupta, A. M., & Alper, H. S. (2013) · Metabolic Engineering · doi:10.1016/j.ymben.2013.07.001 · Cited by 209 · see citing works
Modular control of multiple pathways using engineered orthogonal T7 polymerases
Temme, K., Hill, R. M., Segall-Shapiro, T. H., Moser, F., & Voigt, C. A. (2012) · Nucleic Acids Research · doi:10.1093/nar/gks597 · Cited by 192 · see citing works
Gordon, G. C., Korosh, T. C., Cameron, J. C., Markley, A. L., Begemann, M. B., & Pfleger, B. F. (2016) · Metabolic Engineering · doi:10.1016/j.ymben.2016.07.007 · Cited by 189 · see citing works
Cyanogate: a modular cloning suite for engineering cyanobacteria based on the plant moclo syntax
Vasudevan, R., Gale, G. A. R., Schiavon, A. A., Puzorjov, A., Malin, J., Gillespie, M. D., Vavitsas, K., Zulkower, V., Wang, B., Howe, C. J., et al. (2019) · Plant Physiology · doi:10.1104/pp.18.01401 · Cited by 189 · see citing works
Broad-host-range vector system for synthetic biology and biotechnology in cyanobacteria
Taton, A., Unglaub, F., Wright, N. E., Zeng, W. Y., Paz-Yepes, J., Brahamsha, B., Palenik, B., Peterson, T. C., Haerizadeh, F., Golden, S. S., et al. (2014) · Nucleic Acids Research · doi:10.1093/nar/gku673 · Cited by 186 · see citing works
Isolation of Chlorine-Containing Antibiotic From the Freshwater Cyanobacterium Scytonema hofmanni
Mason, C. P., Edwards, K. R., Carlson, R. E., Pignatello, J. J., Gleason, F. K., & Wood, J. M. (1982) · Science · doi:10.1126/science.6800032 · Cited by 184 · see citing works
CRISPR/Cas system for yeast genome engineering: advances and applications
Šťovíček, V., Holkenbrink, C., & Borodina, I. (2017) · FEMS Yeast Research · doi:10.1093/femsyr/fox030 · Cited by 177 · see citing works
Long, P. F., Dunlap, W. C., Battershill, C. N., & Jaspars, M. (2005) · ChemBioChem · doi:10.1002/cbic.200500210 · Cited by 176 · see citing works
Wide-dynamic-range promoters engineered for cyanobacteria
Huang, H.-H., & Lindblad, P. C. (2013) · Journal of Biological Engineering · doi:10.1186/1754-1611-7-10 · Cited by 172 · see citing works
DP4-AI automated NMR data analysis: straight from spectrometer to structure
Howarth, A., Ermanis, K., & Goodman, J. M. (2020) · Chemical Science · doi:10.1039/d0sc00442a · Cited by 170 · see citing works
Linington, R. G., Clark, B. R., Trimble, E. E., Almanza, A., Ureña, L.-D., Kyle, D. E., & Gerwick, W. H. (2008) · Journal of Natural Products · doi:10.1021/np8003529 · Cited by 167 · see citing works
Englund, E., Liang, F., & Lindberg, P. (2016) · Scientific Reports · doi:10.1038/srep36640 · Cited by 166 · see citing works
Biological targets and mechanisms of action of natural products from marine cyanobacteria
Salvador‐Reyes, L. A., & Luesch, H. (2015) · Natural Product Reports · doi:10.1039/c4np00104d · Cited by 165 · see citing works
Self-citationCyanobacterial metabolic engineering for biofuel and chemical production
Oliver, N. J., Rabinovitch-Deere, C. A., Carroll, A. L., Nozzi, N. E., Case, A. E., & Atsumi, S. (2016) · Current Opinion in Chemical Biology · doi:10.1016/j.cbpa.2016.08.023 · Cited by 165 · see citing works
Na, D., & Lee, D. (2010) · Bioinformatics · doi:10.1093/bioinformatics/btq458 · Cited by 162 · see citing works
Genetic platforms for heterologous expression of microbial natural products
Zhang, J. J., Tang, X., & Moore, B. S. (2019) · Natural Product Reports · doi:10.1039/c9np00025a · Cited by 162 · see citing works
Enhanced limonene production in cyanobacteria reveals photosynthesis limitations
Wang, X., Liu, W., Xin, C., Zheng, Y., Cheng, Y., Sun, S., Li, R., Zhu, X., Dai, S. Y., Rentzepis, P. M., et al. (2016) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1613340113 · Cited by 161 · see citing works
The Escherichia coli RNA polymerase α subunit: structure and function
Ebright, R. H. (1995) · Current Opinion in Genetics & Development · doi:10.1016/0959-437x(95)80008-5 · Cited by 159 · see citing works
Ribosomal Synthesis of Tricyclic Depsipeptides in Bloom‐Forming Cyanobacteria
Ziemert, N., Ishida, K., Liaimer, A., Hertweck, C., & Dittmann, E. (2008) · Angewandte Chemie International Edition · doi:10.1002/anie.200802730 · Cited by 157 · see citing works
Mazard, S., Penesyan, A., Ostrowski, M. D., Paulsen, I. T., & Egan, S. (2016) · Marine Drugs · doi:10.3390/md14050097 · Cited by 154 · see citing works
Genome Mining as New Challenge in Natural Products Discovery
Albarano, L., Esposito, R., Ruocco, N., & Costantini, M. (2020) · Marine Drugs · doi:10.3390/md18040199 · Cited by 152 · see citing works
Ramaswamy, A. V., Sorrels, C. M., & Gerwick, W. H. (2007) · Journal of Natural Products · doi:10.1021/np0704250 · Cited by 145 · see citing works
Nakahira, Y., Ogawa, A., Asano, H., Oyama, T., & Tozawa, Y. (2013) · Plant and Cell Physiology · doi:10.1093/pcp/pct115 · Cited by 143 · see citing works
Synergistic allelochemicals from a freshwater cyanobacterium
Leão, P. N., Pereira, A. R., Liu, W., Ng, J., Pevzner, P. A., Dorrestein, P. C., König, G. M., Vasconcelos, V. M., & Gerwick, W. H. (2010) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0914343107 · Cited by 142 · see citing works
Advances in exploring the therapeutic potential of marine natural products
Liang, X., Luo, D., & Luesch, H. (2019) · Pharmacological Research · doi:10.1016/j.phrs.2019.104373 · Cited by 130 · see citing works
Self-citationTransformation-associated recombination (TAR) cloning for genomics studies and synthetic biology
Kouprina, N., & Larionov, V. (2016) · Chromosoma · doi:10.1007/s00412-016-0588-3 · Cited by 127 · see citing works
Ma, A. T., Schmidt, C. M., & Golden, J. W. (2014) · Applied and Environmental Microbiology · doi:10.1128/aem.01697-14 · Cited by 126 · see citing works
Pereira, A. R., Kale, A. J., Fenley, A. T., Byrum, T., Debonsi, H. M., Gilson, M. K., Valeriote, F. A., Moore, B. S., & Gerwick, W. H. (2012) · ChemBioChem · doi:10.1002/cbic.201200007 · Cited by 125 · see citing works
Ribosomal Synthesis of Tricyclic Depsipeptides in Bloom‐Forming Cyanobacteria
Ziemert, N., Ishida, K., Liaimer, A., Hertweck, C., & Dittmann, E. (2008) · Angewandte Chemie · doi:10.1002/ange.200802730 · Cited by 125 · see citing works
Kaczmarzyk, D., Cengic, I., Yao, L., & Hudson, E. P. (2018) · Metabolic Engineering · doi:10.1016/j.ymben.2017.11.014 · Cited by 120 · see citing works
Niu, T., Lin, G., Xie, L.-R., Wang, Z.-Q., Xing, W., Zhang, J., & Zhang, C. (2018) · ACS Synthetic Biology · doi:10.1021/acssynbio.8b00437 · Cited by 119 · see citing works
Choi, H., Mascuch, S. J., Villa, F. A., Byrum, T., Teasdale, M. E., Smith, J. E., Preskitt, L. B., Rowley, D. C., Gerwick, L. G., & Gerwick, W. H. (2012) · Chemistry & Biology · doi:10.1016/j.chembiol.2012.03.014 · Cited by 117 · see citing works
Engineering cyanobacteria for production of terpenoids
Lin, P.-C., & Pakrasi, H. B. (2018) · Planta · doi:10.1007/s00425-018-3047-y · Cited by 109 · see citing works
Antifungal Compounds from Cyanobacteria
Shishido, T. K., Humisto, A., Jokela, J., Liu, L., Wahlsten, M., Tamrakar, A., Fewer, D. P., Permi, P., Andreote, A. P. D., Fiore, M. F., et al. (2015) · Marine Drugs · doi:10.3390/md13042124 · Cited by 108 · see citing works
Brahamsha, B., & Haselkorn, R. (1991) · Journal of Bacteriology · doi:10.1128/jb.173.8.2442-2450.1991 · Cited by 108 · see citing works
Tang, W., & van der Donk, W. A. (2012) · Biochemistry · doi:10.1021/bi300255s · Cited by 105 · see citing works
Combinatorial pathway optimization for streamlined metabolic engineering
Jeschek, M., Gerngross, D., & Panke, S. (2017) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2017.06.014 · Cited by 104 · see citing works
Direct Pathway Cloning (DiPaC) to unlock natural product biosynthetic potential
Greunke, C., Duell, E. R., D’Agostino, P. M., Glöckle, A., Lamm, K., & Gulder, T. A. M. (2018) · Metabolic Engineering · doi:10.1016/j.ymben.2018.03.010 · Cited by 104 · see citing works
Tsukada, K., Shinki, S., Kaneko, A., Murakami, K., Irie, K., Murai, M., Miyoshi, H., Dan, S., Kawaji, K., Hayashi, H., et al. (2020) · Nature Communications · doi:10.1038/s41467-020-15664-4 · Cited by 103 · see citing works
Approaches and Recent Developments for the Commercial Production of Semi-synthetic Artemisinin
Kung, S. H., Lund, S., Murarka, A., McPhee, D. J., & Paddon, C. J. (2018) · Frontiers in Plant Science · doi:10.3389/fpls.2018.00087 · Cited by 101 · see citing works
Wang, B., Eckert, C. A., Maness, P., & Yu, J. (2017) · ACS Synthetic Biology · doi:10.1021/acssynbio.7b00297 · Cited by 99 · see citing works
Promoter engineering strategies for the overproduction of valuable metabolites in microbes
Jin, L.-Q., Jin, W.-R., Ma, Z.-C., Shen, Q., Cai, X., Liu, Z., & Zheng, Y. (2019) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-019-10172-y · Cited by 99 · see citing works
Liu, D., & Pakrasi, H. B. (2018) · Microbial Cell Factories · doi:10.1186/s12934-018-0897-8 · Cited by 98 · see citing works
Promoter Architecture and Promoter Engineering in Saccharomyces cerevisiae
Tang, H., Wu, Y., Deng, J., Chen, N., Zheng, Z., Wei, Y., Luo, X., & Keasling, J. D. (2020) · Metabolites · doi:10.3390/metabo10080320 · Cited by 97 · see citing works
Replication of the broad host range plasmid RSF1010: requirement for three plasmid-encoded proteins.
Scherzinger, E., Bagdasarian, M., Scholz, P., Lurz, R., Rückert, B., & Bagdasarian, M. (1984) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.81.3.654 · Cited by 95 · see citing works
Ziemert, N., Ishida, K., Weiz, A. R., Hertweck, C., & Dittmann, E. (2010) · Applied and Environmental Microbiology · doi:10.1128/aem.02858-09 · Cited by 95 · see citing works
Nitrogen economy of endolithic microbial communities in hot and cold deserts
Friedmann, E. I., & Kibler, A. P. (1980) · Microbial Ecology · doi:10.1007/bf02010548 · Cited by 92 · see citing works
Heterologous production of small molecules in the optimized Streptomyces hosts
Myronovskyi, M., & Luzhetskyy, A. N. (2019) · Natural Product Reports · doi:10.1039/c9np00023b · Cited by 92 · see citing works
Frigaard, N., Sakuragi, Y., & Bryant, D. A. (2004) · Humana Press eBooks · doi:10.1385/1-59259-799-8:325 · Cited by 91 · see citing works
Transcriptional regulation with CRISPR-Cas9: principles, advances, and applications
Didovyk, A., Borek, B., Tsimring, L., & Hasty, J. (2016) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2016.06.003 · Cited by 90 · see citing works
Editing streptomycete genomes in the CRISPR/Cas9 age
Alberti, F., & Corre, C. (2019) · Natural Product Reports · doi:10.1039/c8np00081f · Cited by 90 · see citing works
Bu, Q., Yu, P., Wang, J., Li, Z., Chen, X.-A., Mao, X., & Li, Y. (2019) · Microbial Cell Factories · doi:10.1186/s12934-019-1055-7 · Cited by 89 · see citing works
Ding, M., Yan, H.-F., Li, L., Zhai, F., Shang, L., Yin, Z., & Yuan, Y. (2014) · PLoS ONE · doi:10.1371/journal.pone.0109348 · Cited by 88 · see citing works
Ongley, S. E., Bian, X., Zhang, Y., Chau, R., Gerwick, W. H., Müller, R., & Neilan, B. A. (2013) · ACS Chemical Biology · doi:10.1021/cb400189j · Cited by 86 · see citing works
Highly Diverse Cyanobactins in Strains of the Genus Anabaena
Leikoski, N., Fewer, D. P., Jokela, J., Wahlsten, M., Rouhiainen, L., & Sivonen, K. (2009) · Applied and Environmental Microbiology · doi:10.1128/aem.01061-09 · Cited by 86 · see citing works
D'Agostino, P. M., & Gulder, T. A. M. (2018) · ACS Synthetic Biology · doi:10.1021/acssynbio.8b00151 · Cited by 84 · see citing works
Ng, A. H., Berla, B. M., & Pakrasi, H. B. (2015) · Applied and Environmental Microbiology · doi:10.1128/aem.01349-15 · Cited by 83 · see citing works
Brilisauer, K., Rapp, J., Rath, P., Schöllhorn, A., Bleul, L., Weiß, E., Stahl, M., Grond, S., & Forchhammer, K. (2019) · Nature Communications · doi:10.1038/s41467-019-08476-8 · Cited by 83 · see citing works
Leão, T., Castelão, G., Korobeynikov, A., Monroe, E. A., Podell, S. B., Glukhov, E., Allen, E. E., Gerwick, W. H., & Gerwick, L. G. (2017) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1618556114 · Cited by 82 · see citing works
Photosynthetic Production of Sunscreen Shinorine Using an Engineered Cyanobacterium
Yang, G., Cozad, M. A., Holland, D. A., Zhang, Y., Luesch, H., & Ding, Y. (2018) · ACS Synthetic Biology · doi:10.1021/acssynbio.7b00397 · Cited by 82 · see citing works
Self-citationHijacking CRISPR-Cas for high-throughput bacterial metabolic engineering: advances and prospects
Mougiakos, I., Bosma, E. F., Ganguly, J., John van der, O. O. S. T., & van Kranenburg, R. (2018) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2018.01.002 · Cited by 82 · see citing works
Seo, S. W., Yang, J., Cho, H., Yang, J., Kim, S., Park, J. M., Kim, S., & Jung, G. Y. (2014) · Scientific Reports · doi:10.1038/srep04515 · Cited by 78 · see citing works
Engineering of a green‐light inducible gene expression system in S ynechocystis sp. PCC 6803
Abe, K., Miyake, K., Nakamura, M., Kojima, K., Ferri, S. R., Ikebukuro, K., & Sode, K. (2013) · Microbial Biotechnology · doi:10.1111/1751-7915.12098 · Cited by 76 · see citing works
Katoch, M., Mazmouz, R., Chau, R., Pearson, L. A., Pickford, R., & Neilan, B. A. (2016) · Applied and Environmental Microbiology · doi:10.1128/aem.01632-16 · Cited by 76 · see citing works
The plasticity of cyanobacterial carbon metabolism
Xiong, W., Cano, M., Wang, B., Douchi, D., & Yu, J. (2017) · Current Opinion in Chemical Biology · doi:10.1016/j.cbpa.2017.09.004 · Cited by 73 · see citing works
Nguyen, C. T., Dhakal, D., Pham, V. T. T., Nguyen, H. T., & Sohng, J. K. (2020) · Microorganisms · doi:10.3390/microorganisms8040616 · Cited by 73 · see citing works
Self-citationKim, W. J., Lee, S.-M., Um, Y., Sim, S. J., & Woo, H. M. (2017) · Frontiers in Plant Science · doi:10.3389/fpls.2017.00293 · Cited by 70 · see citing works
Zhang, Y., Chen, M., Bruner, S. D., & Ding, Y. (2018) · Frontiers in Microbiology · doi:10.3389/fmicb.2018.01801 · Cited by 70 · see citing works
Self-citationVideau, P., Wells, K. N., Singh, A. J., Gerwick, W. H., & Philmus, B. (2016) · ACS Synthetic Biology · doi:10.1021/acssynbio.6b00038 · Cited by 66 · see citing works
Progress and challenges in engineering cyanobacteria as chassis for light‐driven biotechnology
Hitchcock, A., Hunter, C. N., & Canniffe, D. P. (2019) · Microbial Biotechnology · doi:10.1111/1751-7915.13526 · Cited by 66 · see citing works
Heterologous Production of 4-O-Demethylbarbamide, a Marine Cyanobacterial Natural Product
Kim, E. J., Lee, J. H., Choi, H., Pereira, A. R., Ban, Y. H., Yoo, Y. J., Kim, E., Park, J. W., Sherman, D. H., Gerwick, W. H., et al. (2012) · Organic Letters · doi:10.1021/ol302575h · Cited by 65 · see citing works
Marine Cyanobacteria: A Source of Lead Compounds and their Clinically-Relevant Molecular Targets
Tan, L. T., & Phyo, M. Y. (2020) · Molecules · doi:10.3390/molecules25092197 · Cited by 65 · see citing works
Shang, J., Zhang, Z., Yin, X., Chen, M., Hao, F., Wang, K., Feng, J., Xu, H., Yin, Y., Tang, H., et al. (2017) · Environmental Microbiology · doi:10.1111/1462-2920.13972 · Cited by 64 · see citing works
Self-citationGeerts, D., Bovy, A. G., de Vrieze, G., Borrias, M., & Weisbeek, P. J. (1995) · Microbiology · doi:10.1099/13500872-141-4-831 · Cited by 62 · see citing works
Harvey, C. J. B., Puglisi, J. D., Pande, V. S., Cane, D. E., & Khosla, C. (2012) · Journal of the American Chemical Society · doi:10.1021/ja304682q · Cited by 59 · see citing works
Weiz, A. R., Ishida, K., Makower, A. K., Ziemert, N., Hertweck, C., & Dittmann, E. (2011) · Chemistry & Biology · doi:10.1016/j.chembiol.2011.09.011 · Cited by 59 · see citing works
Onai, K., Morishita, M., Kaneko, T., Tabata, S., & Ishiura, M. (2003) · Molecular Genetics and Genomics · doi:10.1007/s00438-003-0953-9 · Cited by 57 · see citing works
Thuấn, N. H., An, T. T., Shrestha, A., Canh, N. X., Sohng, J. K., & Dhakal, D. (2019) · Frontiers in Chemistry · doi:10.3389/fchem.2019.00604 · Cited by 56 · see citing works
Self-citationEngineering Saccharomyces cerevisiae for production of simvastatin
Bond, C. M., & Tang, Y. (2018) · Metabolic Engineering · doi:10.1016/j.ymben.2018.09.005 · Cited by 55 · see citing works
Bishé, B., Taton, A., & Golden, J. W. (2019) · iScience · doi:10.1016/j.isci.2019.09.002 · Cited by 55 · see citing works
Emerging molecular biology tools and strategies for engineering natural product biosynthesis
Xu, W., Klumbys, E., Ang, E. L., & Zhao, H. (2019) · Metabolic Engineering Communications · doi:10.1016/j.mec.2019.e00108 · Cited by 55 · see citing works
In Vitro and In Vivo Osteogenic Activity of Largazole
Lee, S., Kwak, H. B., Pi, S., You, H., Byeon, S. R., Ying, Y., Luesch, H., Hong, J., & Kim, S. H. (2011) · ACS Medicinal Chemistry Letters · doi:10.1021/ml1002794 · Cited by 54 · see citing works
Self-citationCummings, M., Peters, A. D., Whitehead, G. F. S., Menon, B. R. K., Micklefield, J., Webb, S. J., & Eriko Takano, A. (2019) · PLoS Biology · doi:10.1371/journal.pbio.3000347 · Cited by 53 · see citing works
Cyanobacterial sigma factors: Current and future applications for biotechnological advances
Srivastava, A., Summers, M. L., & Sobotka, R. (2020) · Biotechnology Advances · doi:10.1016/j.biotechadv.2020.107517 · Cited by 49 · see citing works
A tightly inducible riboswitch system in Synechocystis sp. PCC 6803
Ohbayashi, R., Akai, H., Yoshikawa, H., Hess, W. R., & Watanabe, S. (2016) · The Journal of General and Applied Microbiology · doi:10.2323/jgam.2016.02.002 · Cited by 48 · see citing works
Yan, F., Burgard, C., Popoff, A., Zaburannyi, N., Zipf, G., Maier, J., Bernauer, H. S., Wenzel, S. C., & Müller, R. (2018) · Chemical Science · doi:10.1039/c8sc02046a · Cited by 48 · see citing works
Park, S.-H., Lee, K., Jang, J. W., & Hahn, J. (2018) · ACS Synthetic Biology · doi:10.1021/acssynbio.8b00388 · Cited by 47 · see citing works
Fundamental CRISPR-Cas9 tools and current applications in microbial systems
Tian, P., Wang, J., Shen, X., Rey, J. F., Yuan, Q., & Yan, Y. (2017) · Synthetic and Systems Biotechnology · doi:10.1016/j.synbio.2017.08.006 · Cited by 46 · see citing works
Jin, H., Wang, Y., Idoine, A., & Bhaya, D. (2018) · Frontiers in Microbiology · doi:10.3389/fmicb.2018.01662 · Cited by 46 · see citing works
Sebesta, J., & Peebles, C. A. (2020) · Metabolic Engineering Communications · doi:10.1016/j.mec.2019.e00117 · Cited by 46 · see citing works
Fewer, D. P., Österholm, J., Rouhiainen, L., Jokela, J., Wahlsten, M., & Sivonen, K. (2011) · Applied and Environmental Microbiology · doi:10.1128/aem.05993-11 · Cited by 45 · see citing works
Biomolecules from Fossilized Hot Spring Sinters: Implications for the Search for Life on Mars
Teece, B. L., George, S. C., Djokic, T., Campbell, K. A., Ruff, S. W., & Kranendonk, M. J. V. (2020) · Astrobiology · doi:10.1089/ast.2018.2018 · Cited by 43 · see citing works
[1] Transposon mutagenesis of heterocyst-forming filamentous cyanobacteria
Cohen, M. F., Meeks, J. C., Cai, Y. A., & Wölk, C. P. (1998) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/s0076-6879(98)97003-3 · Cited by 42 · see citing works
Heterologous production of non-ribosomal peptide LLD-ACV in Saccharomyces cerevisiae
Siewers, V., Chen, X., Huang, L., Zhang, J., & Nielsen, J. (2009) · Metabolic Engineering · doi:10.1016/j.ymben.2009.08.002 · Cited by 42 · see citing works
Directing the Heterologous Production of Specific Cyanobacterial Toxin Variants
Liu, T., Mazmouz, R., Ongley, S. E., Chau, R., Pickford, R., Woodhouse, J. N., & Neilan, B. A. (2017) · ACS Chemical Biology · doi:10.1021/acschembio.7b00181 · Cited by 42 · see citing works
Regulatory Tools for Controlling Gene Expression in Cyanobacteria
Gordon, G. C., & Pfleger, B. F. (2018) · Advances in experimental medicine and biology · doi:10.1007/978-981-13-0854-3_12 · Cited by 40 · see citing works
Comprehensive database of secondary metabolites from cyanobacteriaPreprint
Jones, M. R., Pinto, E., Torres, M. D. A., et al. (2020) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2020.04.16.038703 · Cited by 40 · see citing works
Heterologous Expression of Cryptomaldamide in a Cyanobacterial Host
Taton, A., Ecker, A. K., Diaz, B., Moss, N. A., Anderson, B., Reher, R., Leão, T., Simkovsky, R., Dorrestein, P. C., Gerwick, L. G., et al. (2020) · ACS Synthetic Biology · doi:10.1021/acssynbio.0c00431 · Cited by 39 · see citing works
Cai, W., Chen, Q.-Y., Dang, L. H., & Luesch, H. (2017) · ACS Medicinal Chemistry Letters · doi:10.1021/acsmedchemlett.7b00192 · Cited by 36 · see citing works
Self-citationEngineering actinomycetes for biosynthesis of macrolactone polyketides
Dhakal, D., Sohng, J. K., & Pandey, R. P. (2019) · Microbial Cell Factories · doi:10.1186/s12934-019-1184-z · Cited by 36 · see citing works
Self-citationEngineered Production of Hapalindole Alkaloids in the Cyanobacterium Synechococcus sp. UTEX 2973
Knoot, C. J., Khatri, Y., Hohlman, R. M., Sherman, D. H., & Pakrasi, H. B. (2019) · ACS Synthetic Biology · doi:10.1021/acssynbio.9b00229 · Cited by 35 · see citing works
Montaser, R., Paul, V. J., & Luesch, H. (2013) · Organic Letters · doi:10.1021/ol401396u · Cited by 34 · see citing works
Self-citationAl‐Awadhi, F. H., Salvador‐Reyes, L. A., Elsadek, L. A., Ratnayake, R., Chen, Q.-Y., & Luesch, H. (2020) · ACS Chemical Neuroscience · doi:10.1021/acschemneuro.0c00093 · Cited by 34 · see citing works
Self-citationVogel, A. I. M., Lale, R. R., & Hohmann‐Marriott, M. F. (2017) · Journal of Biological Engineering · doi:10.1186/s13036-017-0061-8 · Cited by 33 · see citing works
Development of a cyanobacterial heterologous polyketide production platform
Roulet, J., Taton, A., Golden, J. W., Arabolaza, A. L., Burkart, M. D., & Gramajo, H. C. (2018) · Metabolic Engineering · doi:10.1016/j.ymben.2018.07.013 · Cited by 31 · see citing works
A sustainable route to produce the scytonemin precursor using Escherichia coli
Malla, S., & Sommer, M. O. A. (2014) · Green Chemistry · doi:10.1039/c4gc00118d · Cited by 30 · see citing works
Videau, P., Wells, K. N., Singh, A. J., Eiting, J., Proteau, P. J., & Philmus, B. (2020) · ACS Synthetic Biology · doi:10.1021/acssynbio.9b00334 · Cited by 27 · cited 5× · see citing works
“…Gram-negative bacterium E. coli (Ongley et al., 2013; Schmidt et al., 2005), the Gram-positive bacterium Streptomyces venezuelae (Kim et al., 2012), genetically amenable model cyanobacterial strains (Taton et al., 2020; Videau et al., 2016, 2020; Yang et al., 2018), and yeast (Park et al., 2019).”
BackgroundMethodInfluentialInsecticidal compounds of the biofilm‐forming cyanobacterium Fischerella sp. (ATCC 43239)
Becher, P. G., & Jüttner, F. (2005) · Environmental Toxicology · doi:10.1002/tox.20113 · Cited by 26 · see citing works
Scaffold‐fused riboregulators for enhanced gene activation in Synechocystis sp. PCC 6803
Sakai, Y., Abe, K., Nakashima, S., Ellinger, J. J., Ferri, S. R., Sode, K., & Ikebukuro, K. (2015) · MicrobiologyOpen · doi:10.1002/mbo3.257 · Cited by 25 · see citing works
Al‐Awadhi, F. H., Gao, B., Rezaei, M. A., Kwan, J. C., Li, C., Ye, T., Paul, V. J., & Luesch, H. (2018) · Journal of Medicinal Chemistry · doi:10.1021/acs.jmedchem.8b00885 · Cited by 24 · see citing works
Self-citationOverview of Regulatory Strategies and Molecular Elements in Metabolic Engineering of Bacteria
Wang, T., Ma, X., Du, G., & Chen, J. (2012) · Molecular Biotechnology · doi:10.1007/s12033-012-9514-y · Cited by 23 · see citing works
Kansara, S. G., & Sukhodolets, M. V. (2011) · PLoS ONE · doi:10.1371/journal.pone.0018990 · Cited by 20 · see citing works
Yang, G., Zhang, Y., Lee, N. K., Cozad, M. A., Kearney, S. E., Luesch, H., & Ding, Y. (2017) · Scientific Reports · doi:10.1038/s41598-017-12244-3 · Cited by 20 · see citing works
Self-citationUeno, K., Sakai, Y., Shono, C., Sakamoto, I., Tsukakoshi, K., Hihara, Y., Sode, K., & Ikebukuro, K. (2017) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-017-8570-4 · Cited by 19 · see citing works
Coalition of Biology and Chemistry for Ameliorating Antimicrobial Drug Discovery
Dhakal, D., & Sohng, J. K. (2017) · Frontiers in Microbiology · doi:10.3389/fmicb.2017.00734 · Cited by 18 · see citing works
Self-citationMishra, R., Dhakal, D., Han, J. M., Lim, H. N., Jung, H. J., Yamaguchi, T., & Sohng, J. K. (2019) · Molecules · doi:10.3390/molecules24020244 · Cited by 18 · see citing works
Self-citationLiu, T., Mazmouz, R., Pearson, L. A., & Neilan, B. A. (2019) · ACS Synthetic Biology · doi:10.1021/acssynbio.9b00068 · Cited by 18 · see citing works
Improving the induction fold of riboregulators for cyanobacteria
Sakamoto, I., Abe, K., Kawai, S., Tsukakoshi, K., Sakai, Y., Sode, K., & Ikebukuro, K. (2018) · RNA Biology · doi:10.1080/15476286.2017.1422470 · Cited by 11 · see citing works
Wells, K. N., Videau, P., Nelson, D., Eiting, J., & Philmus, B. J. (2018) · FEMS Microbiology Letters · doi:10.1093/femsle/fny164 · Cited by 8 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Harnessing the potential: Advances in cyanobacterial natural product research and biotechnology
Baunach, M., Guljamow, A., Miguel-Gordo, M., & Dittmann, E. (2024) · Natural Product Reports · doi:10.1039/d3np00045a · Cited by 66 · see citing works
Robles-Bañuelos, B., Durán-Riveroll, L. M., Rangel‐López, E., Pérez-López, H. I., & González‐Maya, L. (2022) · Molecules · doi:10.3390/molecules27154814 · Cited by 54 · see citing works
Xu, Y., Du, X., Yu, X., Jiang, Q., Zheng, K., Xu, J., & Wang, P. (2022) · Marine Drugs · doi:10.3390/md20060341 · Cited by 49 · see citing works
Discovery and Heterologous Expression of Microginins from Microcystis aeruginosa LEGE 91341
Eusébio, N., Castelo‐Branco, R., Sousa, D., Preto, M., D’Agostino, P. M., Gulder, T. A. M., & Leão, P. N. (2022) · ACS Synthetic Biology · doi:10.1021/acssynbio.2c00389 · Cited by 32 · see citing works
Chen, M., Dhakal, D., Eckhardt, C. W., Luesch, H., & Ding, Y. (2025) · Natural Product Reports · doi:10.1039/d5np00009b · Cited by 21 · see citing works
Self-citationBeyond Soil-Dwelling Actinobacteria: Fantastic Antibiotics and Where to Find Them
Santos‐Aberturas, J., & Vior, N. M. (2022) · Antibiotics · doi:10.3390/antibiotics11020195 · Cited by 20 · see citing works
Taton, A., Rohrer, S., Diaz, B., Reher, R., Caraballo‐Rodríguez, A. M., Pierce, M. L., Dorrestein, P. C., Gerwick, L. G., Gerwick, W. H., & Golden, J. W. (2022) · ACS Chemical Biology · doi:10.1021/acschembio.2c00347 · Cited by 17 · see citing works
Incorporation and modification of fatty acids in cyanobacterial natural products biosynthesis
Leão, P. N., Martins, T. P., Abt, K., Reis, J. P. A., Figueiredo, S. A. C., Castelo‐Branco, R., & Freitas, S. (2023) · Chemical Communications · doi:10.1039/d3cc00136a · Cited by 17 · see citing works
Lee, H., Nihan, K., & Kwon, Y. R. (2025) · Marine Drugs · doi:10.3390/md23060233 · Cited by 17 · see citing works
Application of Synthetic Biology Approaches to High-Yield Production of Mycosporine-like Amino Acids
Singh, V. K., Jha, S., Rana, P., Gupta, A., Singh, A. P., Kumari, N., Mishra, S., Singh, P. R., Jaiswal, J., & Sinha, R. P. (2023) · Fermentation · doi:10.3390/fermentation9070669 · Cited by 15 · see citing works
Mishra, R., Kaur, P., Soni, R., Madan, A., Agarwal, P., & Singh, G. (2024) · Plant Physiology and Biochemistry · doi:10.1016/j.plaphy.2024.108744 · Cited by 17 · see citing works
Dhakal, D., Kallifidas, D., Chen, M., Kokkaliari, S., Chen, Q.-Y., Paul, V. J., Ding, Y., & Luesch, H. (2023) · Organic Letters · doi:10.1021/acs.orglett.3c00462 · Cited by 11 · see citing works
Self-citationPhilmus, B. J., Avalon, N. E., Ding, Y., Doering, D. T., Eustáquio, A. S., Gerwick, W. H., Luesch, H., Orjala, J., Sutherland, S., Taton, A., et al. (2025) · Natural Product Reports · doi:10.1039/d5np00016e · Cited by 9 · see citing works
Self-citationDhakal, D., Kokkaliari, S., Rubin, G. M., Paul, V. J., Ding, Y., & Luesch, H. (2022) · Journal of Natural Products · doi:10.1021/acs.jnatprod.2c00782 · Cited by 6 · see citing works
Self-citationDeliberations on Natural Products and Future Directions in the Pharmaceutical Industry
Buntin, K., Ertl, P., Hoepfner, D., Krastel, P., Oakeley, E. J., Pistorius, D., Schuhmann, T., Wong, J., & Petersen, F. (2021) · CHIMIA International Journal for Chemistry · doi:10.2533/chimia.2021.620 · Cited by 5 · see citing works
Antimicrobial activity of Cyanobacteria-derived compounds
Gómez-Espinoza, O., Núñez-Montero, K., & Barrientos, L. (2022) · Elsevier eBooks · doi:10.1016/b978-0-12-821491-6.00006-5 · Cited by 5 · see citing works
Introduction to the Special Issue: “Natural Product Discovery and Development in the Genomic Era”
Baltz, R. H., Ikeda, H., Katz, L., Kim, E., van Wezel, G. P., & Wright, G. D. (2015) · Journal of Industrial Microbiology & Biotechnology · doi:10.1007/s10295-015-1715-5 · Cited by 5 · see citing works
He, H., Gao, K., Mao, B., Shi, G., Li, Y., & Deng, X. (2025) · Biotechnology Journal · doi:10.1002/biot.70162 · Cited by 4 · see citing works
Ma, B., Bu, Y., Huang, J., Liu, Y., Guo, Z., Yu, H., Liang, T., & Wang, D. (2025) · FEMS Microbiology Letters · doi:10.1093/femsle/fnaf102 · Cited by 4 · see citing works
Shen, B., Tang, Y., Baltz, R. H., & González, R. (2021) · Journal of Industrial Microbiology & Biotechnology · doi:10.1093/jimb/kuab030 · Cited by 3 · see citing works
Multichassis Expression of Cyanobacterial and Other Bacterial Biosynthetic Gene Clusters
Dhakal, D., Eckhardt, C. W., Jiang, Y., Luesch, H., & Ding, Y. (2025) · ACS Synthetic Biology · doi:10.1021/acssynbio.5c00390 · Cited by 2 · see citing works
Self-citationHarnessing engineered cyanobacteria for next-generation therapeutics
Bhardwaj, A., & Mishra, A. K. (2026) · Archives of Microbiology · doi:10.1007/s00203-026-04790-0 · Cited by 2 · see citing works
Göbner, L., Gulder, T. A. M., & D’Agostino, P. M. (2026) · Essays in Biochemistry · doi:10.1042/ebc20250044 · Cited by 2 · see citing works
Walshe, J., & Saha, S. K. (2026) · Current Issues in Molecular Biology · doi:10.3390/cimb48060638 · Cited by 1 · see citing works
“The construction of heterologous metabolic pathways in cyanobacteria generally involves the coordinated expression of multiple genes that encode enzymes responsible for sequential biochemical reactions [33].”
InfluentialExploring genetic tools for cyanobacteria and their application for biofuels production
Kufryk, G. I. (2022) · Elsevier eBooks · doi:10.1016/b978-0-323-91202-0.00005-1 · Cited by 1 · see citing works
Das, B., Prusty, A., Dutta, S., Maulik, A., Dahat, Y., Kumar, D., & Tripathy, S. (2024) · Heliyon · doi:10.1016/j.heliyon.2024.e36048 · Cited by 1 · see citing works
Metabolomics: An Approach to Improve Production of Cyanobacterial Natural Products
Singh, V. K., Jha, S., Rana, P., Singh, A. P., Tripathi, R., Kumari, N., Häder, D., & Sinha, R. P. (2025) · Springer eBooks · doi:10.1007/978-981-96-7067-3_19 · Cited by 1 · see citing works
Mangrove cyanobacterial diversity as a source of bioactive natural products
Roy, A. R., Basu, S., Villalobos, S. D. L. S., & Mukherjee, J. (2026) · Current Research in Microbial Sciences · doi:10.1016/j.crmicr.2026.100557 · Cited by 1 · see citing works
Liu, H., Ma, H., Ren, Z., Liu, J., Liu, J., Chen, J., Ma, J., Qi, R., Lin, Z., & Qi, R. (2025) · Journal of environmental chemical engineering · doi:10.1016/j.jece.2025.119957
Lian, X.-D., Lee, J., Kang, S., Lee, J., Guan, Y., Lee, M.-K., Jung, K., & Li, Z. (2026) · Algal Research · doi:10.1016/j.algal.2026.104663
Jayasundara, S., Ali, T., Adeyemi, B., Krishnamoorthy, B., Henard, C. A., Chapman, K. D., & Skellam, E. (2026) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.64898/2026.05.15.725566
Anabaena , a multicellular model for cyanobacterial cell biology and biotechnology
Flores, E., Luque, I., & López‐Igual, R. (2026) · Journal of Bacteriology · doi:10.1128/jb.00376-26
- Authors
- Dhakal, D., Chen, M., Luesch, H., & Ding, Y.
- Affiliations
- University of Florida (US)
- Department of Medicinal Chemistry, Center for Natural Products, Drug Discovery and Development, University of Florida , Gainesville, FL 31610, USA
- Corresponding author
- Luesch, Hendrik
- Ding, Yousong
- Venue
- Journal of Industrial Microbiology and Biotechnology
- Publisher
- Oxford University Press (OUP)
- Published
- 2021-01-25
- Volume
- 48
- Issue
- 3-4
- ISSN
- 1367-5435 (ISSN-L)
- 1476-5535
- PMC
- PMC8210676
- OpenAlex
- W3123060788
- Cited by
- 33
- References
- 171
- Citations by year
- 2026: 7 · 2025: 8 · 2024: 2 · 2023: 5 · 2022: 8 · 2021: 2 · 2015: 1
- FWCI
- 0.54
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Journal of Industrial Microbiology & Biotechnology (publishedVersion, other-oa)
- PubMed Central (publishedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (R01CA172310, R35GM128742)
- University of Florida
- Topics
- Health Sciences › Medicine › Pharmacology › Microbial Natural Products and Biosynthesis
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant biochemistry and biosynthesis
- Keywords
- cyanobacterial natural products, heterologous expression, biosynthetic gene clusters, host engineering, synthetic biology, Escherichia coli, yeast, actinobacteria
- MeSH terms
- Cyanobacteria, Animals, Biological Products, Multigene Family, Genetic Engineering, Humans
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review