Lipid recovery from wet oleaginous microbial biomass for biofuel production: A critical review
Open accessAbstract
Biological lipids derived from oleaginous microorganisms are promising precursors for renewable biofuel productions. Direct lipid extraction from wet cell-biomass is favored because it eliminates the need for costly dehydration. However, the development of a practical and scalable process for extracting lipids from wet cell-biomass is far from ready to be commercialized, instead, requiring intensive research and development to understand the lipid accessibility, mechanisms in mass transfer and establish robust lipid extraction approaches that are practical for industrial applications. This paper aims to present a critical review on lipid recovery in the context of biofuel productions with special attention to cell disruption and lipid mass transfer to support extraction from wet biomass.
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Ethanol as a solvent can provide a reasonable partition coefficient, forcing solute to migrate to the solvent phase.
“Ethanol as an ideal solvent used to extract total lipids could provide a reasonable partition co-efficiency, forcing the solute to migrate to the solvent phase [45].”
Combinations of hexane with alcohols such as ethanol and isopropanol have the potential to more selectively extract hydrophobic lipids while maintaining high extraction yields.
“Combinations of hexane with alcohols, such as ethanol and isopropanol, have the potential to more selectively extract hydrophobic lipids while maintaining high extraction yields of these components (31, 39, 40).”
From Catalytic Hydroprocessing of Single-Cell Oils to Hydrocarbon Fuels: Converting microbial lipids to fuels is a promising approach to replace fossil fuels (2021) · cites this paper as [31]
Dilute acid pretreatment is an effective, low cost, energy efficient and scalable means to recover lipids from wet microbial biomass.
“Researchers at the National Renewable Energy Laboratory (NREL) have shown that dilute acid pretreatment is an effective, low cost, energy efficient and scalable means to recover lipids from wet microbial biomass (31-34).”
From Catalytic Hydroprocessing of Single-Cell Oils to Hydrocarbon Fuels: Converting microbial lipids to fuels is a promising approach to replace fossil fuels (2021) · cites this paper as [31]
Various cell wall lysis methods have been applied to rupture microbial cells for lipid recovery, including high-pressure homogenisation, bead milling, ultrasound, pulsed electric field, osmotic shock, microwave, subcritical water hydrolysis, enzymatic hydrolysis, autolysis, and chemical hydrolysis.
“Various cell wall lysis methods have been applied for this application, such as highpressure homogenisation (HPH), bead milling, ultrasound, pulsed electric field (PEF), osmotic shock, microwave, subcritical water hydrolysis, enzymatic hydrolysis, autolysis and chemical hydrolysis (31).”
From Catalytic Hydroprocessing of Single-Cell Oils to Hydrocarbon Fuels: Converting microbial lipids to fuels is a promising approach to replace fossil fuels (2021) · cites this paper as [31]
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Production of microalgae with high lipid content and their potential as sources of nutraceuticals
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Advanced Mass Balance Characterization and Fractionation of Algal Biomass Composition
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- Authors
- Dong, T., Knoshaug, E. P., Pienkos, P. T., & Laurens, L. M. L.
- Affiliations
- National Laboratory of the Rockies (US)
- Corresponding author
- Laurens, Lieve M.L.
- Venue
- Applied Energy
- Publisher
- Elsevier BV
- Published
- 2016-09
- Volume
- 177
- Pages
- 879-895
- ISSN
- 0306-2619 (ISSN-L)
- OpenAlex
- W2442652975
- Cited by
- 349
- References
- 189
- Citations by year
- 2026: 24 · 2025: 26 · 2024: 38 · 2023: 29 · 2022: 39 · 2021: 39 · 2020: 45 · 2019: 49 · 2018: 34 · 2017: 24 · 2016: 1
- FWCI
- 9.77
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Applied Energy (publishedVersion, cc-by)
- RePEc: Research Papers in Economics (submittedVersion)
- OSTI OAI (U.S. Department of Energy Office of Scientific and Technical Information) (submittedVersion)
- Funders
- DOE Bioenergy Technology Office (DE-AC36-08GO28308)
- Topics
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Physical Sciences › Engineering › Biomedical Engineering › Biofuel production and bioconversion
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Keywords
- lipid extraction, wet biomass, oleaginous microorganisms, biofuel production, cell disruption, dehydration avoidance, industrial scalability
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