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Mechanism of Charging and Supercharging Molecules in Electrospray Ionization
Open accessAbstract
The origin of the extent of charging and the mechanism by which multiply charged ions are formed in electrospray ionization have been hotly debated for over a decade. Many factors can affect the number of charges on an analyte ion. Here, we investigate the extent of charging of poly(propyleneimine) dendrimers (generations 3.0 and 5.0), cytochrome c, poly(ethylene glycol)s, and 1,n-diaminoalkanes formed from solutions of different composition. We demonstrate that in the absence of other factors, the surface tension of the electrospray droplet late in the desolvation process is a significant factor in determining the overall analyte charge. For poly(ethylene glycol)s, 1,n-diaminoalkanes, and poly(propyleneimine) dendrimers electrosprayed from single-component solutions, there is a clear relationship between the analyte charge and the solvent surface tension. Addition of m-nitrobenzyl alcohol (m-NBA) into electrospray solutions increases the charging when the original solution has a lower surface tension than m-NBA, but the degree of charging decreases when this compound is added to water, which has a higher surface tension. Similarly, the charging of cytochrome c ions formed from acidified denaturing solutions generally increases with increasing surface tension of the least volatile solvent. For the dendrimers investigated, there is a strong correlation between the average charge state of the dendrimer and the Rayleigh limiting charge calculated for a droplet of the same size as the analyte molecule and with the surface tension of the electrospray solvent. A bimodal charge distribution is observed for larger dendrimers formed from water/m-NBA solutions, suggesting the presence of more than one conformation in solution. A similar correlation is found between the extent of charging for 1,n-diaminoalkanes and the calculated Rayleigh limiting charge. These results provide strong evidence that multiply charged organic ions are formed by the charged residue mechanism. A significantly smaller extent of charging for both dendrimers and 1,n-diaminoalkanes would be expected if the ion evaporation mechanism played a significant role.
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Cites
CRC Handbook of Chemistry and Physics
(2016) · CRC Press eBooks · doi:10.1201/9781315380476 · Cited by 13,921
Electrospray Ionization for Mass Spectrometry of Large Biomolecules
Fenn, J. B., Mann, M., Meng, C. K., Wong, S. F., & Whitehouse, C. M. (1989) · Science · doi:10.1126/science.2675315 · Cited by 7,634
Nist chemistry webbook, nist standard reference database number 69
Linstorm, P. (1998) · Medical Entomology and Zoology · Cited by 4,606
Dole, M., Mack, L. L., Hines, R. L., Mobley, R. C., Ferguson, L. D., & Alice, M. B. (1968) · The Journal of Chemical Physics · doi:10.1063/1.1670391 · Cited by 1,613
Physical and thermodynamic properties of pure chemicals: data compilation
(1990) · Choice Reviews Online · doi:10.5860/choice.27-3319 · Cited by 1,292
On the evaporation of small ions from charged droplets
Iribarne, J. V., & Thomson, B. A. (1976) · The Journal of Chemical Physics · doi:10.1063/1.432536 · Cited by 1,281
Int. J. Mass Spectrom. Ion Processes
(1990) · International Journal of Mass Spectrometry and Ion Processes · doi:10.1016/0168-1176(90)80022-u · Cited by 1,003
Electron Capture Dissociation for Structural Characterization of Multiply Charged Protein Cations
Zubarev, R. A., Horn, D. M., Fridriksson, E. K., Kelleher, N. L., Kruger, N. A., Lewis, M. A., Carpenter, B. K., & McLafferty, F. W. (2000) · Analytical Chemistry · doi:10.1021/ac990811p · Cited by 986
Increased thermal stability of proteins in the presence of sugars and polyols
Back, J. F., Oakenfull, D., & Smith, M. B. (1979) · Biochemistry · doi:10.1021/bi00590a025 · Cited by 757
Probing conformational changes in proteins by mass spectrometry
Chowdhury, S. K., Katta, V., & Chait, B. T. (1990) · Journal of the American Chemical Society · doi:10.1021/ja00180a074 · Cited by 646
Fernandez de la Mora, J. (2000) · Analytica Chimica Acta · doi:10.1016/s0003-2670(99)00601-7 · Cited by 528
Ion formation from charged droplets: Roles of geometry, energy, and time
Fenn, J. B. (1993) · Journal of the American Society for Mass Spectrometry · doi:10.1016/1044-0305(93)85014-o · Cited by 518
Some Developments in Nuclear Magnetic Resonance of Solids
Chmelka, B. F., & Pines, A. (1989) · Science · doi:10.1126/science.246.4926.71 · Cited by 379
Some tenets pertaining to electrospray ionization mass spectrometry
Cole, R. B. (2000) · Journal of Mass Spectrometry · doi:10.1002/1096-9888(200007)35:7<763::aid-jms16>3.0.co;2-# · Cited by 365
On the mechanisms by which the charged droplets produced by electrospray lead to gas phase ions
Kebarle, P., & Peschke, M. (2000) · Analytica Chimica Acta · doi:10.1016/s0003-2670(99)00598-x · Cited by 352
Schnier, P. D., Gross, D. S., & Williams, E. R. (1995) · Journal of the American Society for Mass Spectrometry · doi:10.1016/1044-0305(95)00532-3 · Cited by 258
Self-citationSze, S. K., Ge, Y., Oh, H., & McLafferty, F. W. (2002) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.251691898 · Cited by 250
Konermann, L., & Douglas, D. J. (1997) · Biochemistry · doi:10.1021/bi971266u · Cited by 239
Supercharged Protein and Peptide Ions Formed by Electrospray Ionization
Iavarone, A. T., Jurchen, J. C., & Williams, E. R. (2001) · Analytical Chemistry · doi:10.1021/ac001251t · Cited by 229
Self-citationIavarone, A. T., Jurchen, J. C., & Williams, E. R. (2000) · Journal of the American Society for Mass Spectrometry · doi:10.1016/s1044-0305(00)00169-0 · Cited by 199
Self-citationProton Transfer Reactivity of Large Multiply Charged Ions
Williams, E. R. (1996) · Journal of Mass Spectrometry · doi:10.1002/(sici)1096-9888(199608)31:8<831::aid-jms392>3.0.co;2-7 · Cited by 195
Self-citationHigh-resolution tandem FT mass spectrometry above 10 kDa
McLafferty, F. W. (1994) · Accounts of Chemical Research · doi:10.1021/ar00047a009 · Cited by 194
Effects of Anions on the Positive Ion Electrospray Ionization Mass Spectra of Peptides and Proteins
Mirza, U. A., & Chait, B. T. (1994) · Analytical Chemistry · doi:10.1021/ac00090a017 · Cited by 188
Structure and Conformation of DAB Dendrimers in Solution via Multidimensional NMR Techniques
Chai, M., Niu, Y., Youngs, W. J., & Rinaldi, P. L. (2001) · Journal of the American Chemical Society · doi:10.1021/ja002824m · Cited by 163
Desolvation of ions and molecules in thermospray mass spectrometry
Schmelzeisen-Redeker, G., Bütfering, L., & Röllgen, F. W. (1989) · International Journal of Mass Spectrometry and Ion Processes · doi:10.1016/0168-1176(89)85004-9 · Cited by 163
Origin and number of charges observed on multiply-protonated native proteins produced by ESI
Felitsyn, N. M., Peschke, M., & Kebarle, P. (2002) · International Journal of Mass Spectrometry · doi:10.1016/s1387-3806(02)00588-2 · Cited by 157
Reid, G. E., Wu, J., Chrisman, P. A., Wells, J. M., & McLuckey, S. A. (2001) · Analytical Chemistry · doi:10.1021/ac0101095 · Cited by 155
Jockusch, R. A., Schnier, P. D., Price, W. D., Strittmatter, E. F., Demirev, P. A., & Williams, E. R. (1997) · Analytical Chemistry · doi:10.1021/ac960804q · Cited by 136
Self-citationUnit resolution mass spectra of 112 kDa molecules with 3 Da accuracy
Kelleher, N. L., Senko, M. W., Siegel, M. M., & McLafferty, F. W. (1997) · Journal of the American Society for Mass Spectrometry · doi:10.1016/s1044-0305(97)00060-3 · Cited by 122
Supercharging in electrospray ionization: effects on signal and charge
Iavarone, A. T., & Williams, E. R. (2002) · International Journal of Mass Spectrometry · doi:10.1016/s1387-3806(02)00587-0 · Cited by 121
Self-citationCole, R. B., & Harrata, A. K. (1993) · Journal of the American Society for Mass Spectrometry · doi:10.1016/1044-0305(93)85016-q · Cited by 118
Declustering and fragmentation of protein ions from an electrospray ion source
Thomson, B. A. (1997) · Journal of the American Society for Mass Spectrometry · doi:10.1016/s1044-0305(97)00129-3 · Cited by 82
Hydration of gas-phase ions formed by electrospray ionization
Rodriguez‐Cruz, S. E., Klassen, J. S., & Williams, E. R. (1999) · Journal of the American Society for Mass Spectrometry · doi:10.1016/s1044-0305(99)00068-9 · Cited by 81
Self-citationSolvent Dependence of the Hydrodynamical Volume of Dendrimers with a Rubicene Core
Backer, S. D., Prinzie, Y., Verheijen, W., Smet, M., Desmedt, K., Dehaen, W., & Schryver, F. C. D. (1998) · The Journal of Physical Chemistry A · doi:10.1021/jp9734336 · Cited by 71
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Hall, Z., Politis, A., Bush, M. F., Smith, L. J., & Robinson, C. V. (2012) · Journal of the American Chemical Society · doi:10.1021/ja2096859 · Cited by 267
Top‐down proteomics in health and disease: Challenges and opportunities
Gregorich, Z. R., & Ge, Y. (2014) · PROTEOMICS · doi:10.1002/pmic.201300432 · Cited by 217
Valeja, S. G., Kaiser, N. K., Xian, F., Hendrickson, C. L., Rouse, J. C., & Marshall, A. G. (2011) · Analytical Chemistry · doi:10.1021/ac202429c · Cited by 67
Fragmentation of Integral Membrane Proteins in the Gas Phase
Skinner, O. S., Catherman, A. D., Early, B. P., Thomas, P. M., Compton, P. D., & Kelleher, N. L. (2014) · Analytical Chemistry · doi:10.1021/ac500864w · Cited by 29
Metadata
- Authors
- Iavarone, A. T., & Williams, E. R.
- Venue
- Journal of the American Chemical Society
- Publisher
- American Chemical Society (ACS)
- Published
- 2003-02-01
- Volume
- 125
- Issue
- 8
- Pages
- 2319-2327
- ISSN
- 0002-7863 (ISSN-L)
- 1520-5126
- OpenAlex
- W1992276902
- Cited by
- 324
- References
- 34
- FWCI
- 3.79
- Type
- journal article
- Language
- en
- Open access
- green
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