Cited by 70Advanced Proteomics Techniques and ApplicationsAdvanced Biosensing Techniques and ApplicationsAnalytical chemistry methods development

Specific Enrichment of Phosphoproteins Using Functionalized Multivalent Nanoparticles

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Hwang, Leekyoung, Ayaz-Guner, Serife, Gregorich, Zachery R., Cai, Wenxuan, Valeja, Santosh G., Jin, Song, Ge, Ying·2015·Journal of the American Chemical Society

Abstract

Analysis of protein phosphorylation remains a significant challenge due to the low abundance of phosphoproteins and the low stoichiometry of phosphorylation, which requires effective enrichment of phosphoproteins. Here we have developed superparamagnetic nanoparticles (NPs) whose surface is functionalized by multivalent ligand molecules that specifically bind to the phosphate groups on any phosphoproteins. These NPs enrich phosphoproteins from complex cell and tissue lysates with high specificity as confirmed by SDS-PAGE analysis with a phosphoprotein-specific stain and mass spectrometry analysis of the enriched phosphoproteins. This method enables universal and effective capture, enrichment, and detection of intact phosphoproteins toward a comprehensive analysis of the phosphoproteome.

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  1. [3]Nita-Lazar A Saito-Benz H White FM Proteomics 2008 8 4433 18846511 PMC2701620
  2. [10]Aubin-Tam M-E Hamad-Schifferli K Biomed. Mater 2008 3 034001 18689927

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Authors
Hwang, L., Ayaz-Guner, S., Gregorich, Z. R., Cai, W., Valeja, S. G., Jin, S., & Ge, Y.
Affiliations
  • University of Wisconsin–Madison (US)
  • Department of Chemistry, ‡Department of Cell and Regenerative Biology, §Molecular and Cellular Pharmacology Program, and ∥Human Proteomics Program, University of Wisconsin−Madison, Madison, Wisconsin 53719, United States
Corresponding author
  • Jin, Song
  • Ge, Ying
Venue
Journal of the American Chemical Society
Published
2015-02-11
Volume
137
Issue
7
Pages
2432-2435
ISSN
  • 0002-7863 (ISSN-L)
  • 1520-5126
OpenAlex
W2326828010
Cited by
70
References
36
Citations by year
2026: 4 · 2025: 1 · 2024: 6 · 2023: 4 · 2022: 6 · 2021: 3 · 2020: 9 · 2019: 6 · 2018: 8 · 2017: 10 · 2016: 9 · 2015: 4
FWCI
4.60
Type
journal article
Language
en
Open access
Locations
  • Journal of the American Chemical Society (publishedVersion)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion)
Funders
  • National Heart, Lung, and Blood Institute (R01 HL096971, R01 HL109810)
  • National Institute of Biomedical Imaging and Bioengineering (R21 EB013847)
Topics
  • Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Advanced Biosensing Techniques and Applications
  • Physical Sciences › Chemistry › Analytical Chemistry › Analytical chemistry methods development
Keywords
phosphoprotein enrichment, protein phosphorylation, superparamagnetic nanoparticles, multivalent ligands, phosphoproteome, sample preparation
MeSH terms
Ligands, Phosphoproteins, Magnetite Nanoparticles

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