Antioxidant assays for plant and food components
Abstract
Recently, research on natural antioxidants has become increasingly active in various fields. Accordingly, numerous articles on natural antioxidants, including polyphenols, flavonoids, vitamins, and volatile chemicals, have been published. Assays developed to evaluate the antioxidant activity of plants and food constituents vary. Therefore, to investigate the antioxidant activity of chemical(s), choosing an adequate assay based on the chemical(s) of interest is critical. There are two general types of assays widely used for different antioxidant studies. One is an assay associated with lipid peroxidations, including the thiobarbituric acid assay (TBA), malonaldehyde/high-performance liquid chromatography (MA/HPLC) assay, malonaldehyde/gas chromatography (MA/GC) assay, beta-carotene bleaching assay, and conjugated diene assay. Other assays are associated with electron or radical scavenging, including the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assay, ferric reducing/antioxidant power (FRAP) assay, ferrous oxidation-xylenol orange (FOX) assay, ferric thiocyanate (FTC) assay, and aldehyde/carboxylic acid (ACA) assay. In this review, assays used recently were selected for extended discussion, including discussion of the mechanisms underlying each assay and its application to various plants and foods.
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The aldehyde/carboxylic acid assay is convenient for evaluating the effects of antioxidants against slow oxidation phenomena occurring over prolonged periods of time, as shown in the shelf life of foods.
“This assay is convenient for evaluating the effects of antioxidants against slow oxidation phenomena occurring over prolonged periods of time, as shown in the shelf life of foods [59].”
From Antioxidant and Anti-Inflammatory Activities of Essential Oils: A Short Review (2010) · cites this paper as [59]
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Carocho, M., & Ferreira, I. C. F. R. (2013) · Food and Chemical Toxicology · doi:10.1016/j.fct.2012.09.021 · Cited by 1,661 · see citing works
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“Trolox® equivalent antioxidant capacity (TEAC) or ABTS [2,2-Azinobis(3-ethylbenzothiazoline-6 sulphonic acid)] This method relies on the reduction of the blue-green cation radical of ABTS+•, by measuring the reduction of the radical cation as the percentage of inhibition of absorbance at 734 nm.”
BackgroundMethodInfluentialNatural and synthetic antioxidants: An updated overview
Augustyniak, A., Bartosz, G., Čipak, A., Duburs, G., Horáková, L., Łuczaj, W., Majekova, M., Odysseos, A. D., Rackova, L., Skrzydlewska, E., et al. (2010) · Free Radical Research · doi:10.3109/10715762.2010.508495 · Cited by 319 · see citing works
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- Authors
- Moon, J.-K., & Shibamoto, T.
- Affiliations
- University of California, Davis (US)
- Corresponding author
- Shibamoto, Takayuki
- Venue
- Journal of Agricultural and Food Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2009-01-30
- Volume
- 57
- Issue
- 5
- Pages
- 1655-1666
- ISSN
- 0021-8561 (ISSN-L)
- 1520-5118
- OpenAlex
- W2049000879
- Cited by
- 958
- References
- 180
- Citations by year
- 2026: 14 · 2025: 44 · 2024: 66 · 2023: 63 · 2022: 78 · 2021: 79 · 2020: 66 · 2019: 49 · 2018: 47 · 2017: 53 · 2016: 49 · 2015: 59 · 2014: 57 · 2013: 61 · 2012: 60
- FWCI
- 37.31
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Agricultural and Food Chemistry (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Biochemistry › Phytochemicals and Antioxidant Activities
- Health Sciences › Medicine › Biochemistry › Antioxidant Activity and Oxidative Stress
- Physical Sciences › Chemistry › Organic Chemistry › Free Radicals and Antioxidants
- Keywords
- antioxidant activity assay, natural antioxidants, lipid peroxidation, free radical scavenging, DPPH assay, ABTS radical scavenging, FRAP, thiobarbituric acid assay, malondialdehyde, conjugated dienes, ferric thiocyanate assay, polyphenols, flavonoids, vitamins, plant constituents, food components
- MeSH terms
- Antioxidants, Chemistry Techniques, Analytical, Food Analysis, Plant Extracts, Plants
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