Cited by 958Phytochemicals and Antioxidant ActivitiesAntioxidant Activity and Oxidative StressFree Radicals and Antioxidants

Antioxidant assays for plant and food components

Moon, Joon-Kwan, Shibamoto, Takayuki·2009·Journal of Agricultural and Food Chemistry

Abstract

Recently, research on natural antioxidants has become increasingly active in various fields. Accordingly, numerous articles on natural antioxidants, including polyphenols, flavonoids, vitamins, and volatile chemicals, have been published. Assays developed to evaluate the antioxidant activity of plants and food constituents vary. Therefore, to investigate the antioxidant activity of chemical(s), choosing an adequate assay based on the chemical(s) of interest is critical. There are two general types of assays widely used for different antioxidant studies. One is an assay associated with lipid peroxidations, including the thiobarbituric acid assay (TBA), malonaldehyde/high-performance liquid chromatography (MA/HPLC) assay, malonaldehyde/gas chromatography (MA/GC) assay, beta-carotene bleaching assay, and conjugated diene assay. Other assays are associated with electron or radical scavenging, including the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assay, ferric reducing/antioxidant power (FRAP) assay, ferrous oxidation-xylenol orange (FOX) assay, ferric thiocyanate (FTC) assay, and aldehyde/carboxylic acid (ACA) assay. In this review, assays used recently were selected for extended discussion, including discussion of the mechanisms underlying each assay and its application to various plants and foods.

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Crossref · 10.1021/jf803537k

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  • The aldehyde/carboxylic acid assay is convenient for evaluating the effects of antioxidants against slow oxidation phenomena occurring over prolonged periods of time, as shown in the shelf life of foods.

    “This assay is convenient for evaluating the effects of antioxidants against slow oxidation phenomena occurring over prolonged periods of time, as shown in the shelf life of foods [59].”

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Authors
Moon, J.-K., & Shibamoto, T.
Affiliations
University of California, Davis (US)
Corresponding author
Shibamoto, Takayuki
Venue
Journal of Agricultural and Food Chemistry
Published
2009-01-30
Volume
57
Issue
5
Pages
1655-1666
ISSN
  • 0021-8561 (ISSN-L)
  • 1520-5118
OpenAlex
W2049000879
Cited by
958
References
180
Citations by year
2026: 14 · 2025: 44 · 2024: 66 · 2023: 63 · 2022: 78 · 2021: 79 · 2020: 66 · 2019: 49 · 2018: 47 · 2017: 53 · 2016: 49 · 2015: 59 · 2014: 57 · 2013: 61 · 2012: 60
FWCI
37.31
Type
journal article
Language
en
Open access
closed
Locations
  • Journal of Agricultural and Food Chemistry (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Biochemistry › Phytochemicals and Antioxidant Activities
  • Health Sciences › Medicine › Biochemistry › Antioxidant Activity and Oxidative Stress
  • Physical Sciences › Chemistry › Organic Chemistry › Free Radicals and Antioxidants
Keywords
antioxidant activity assay, natural antioxidants, lipid peroxidation, free radical scavenging, DPPH assay, ABTS radical scavenging, FRAP, thiobarbituric acid assay, malondialdehyde, conjugated dienes, ferric thiocyanate assay, polyphenols, flavonoids, vitamins, plant constituents, food components
MeSH terms
Antioxidants, Chemistry Techniques, Analytical, Food Analysis, Plant Extracts, Plants

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