Fucoidan Induces Apoptosis through Activation of Caspase-8 on Human Breast Cancer MCF-7 Cells
Abstract
Fucoidan is an active component of seaweed that has been shown to inhibit proliferation and induce apoptotic cell death in several tumor cells. However, the detailed mechanisms underlying this process have not yet been elucidated. In the present report, we investigated the effect of fucoidan on the induction of apoptosis in human breast cancer MCF-7 cells. Our data demonstrated that fucoidan reduced the viable cell number of MCF-7 cells in a dose- and time-dependent manner. In contrast, fucoidan did not affect the viable cell number of normal human mammary epithelial cells. Results from the apoptosis assay demonstrated that fucoidan induced internucleosomal DNA fragmentation, chromatin condensation, activation of caspase-7, -8, and -9, and cleavage of poly(ADP ribose) polymerase. Furthermore, expression of Bid was decreased, whereas truncated Bid was increased by fucoidan treatment. There was also a decline in cytosolic Bax and a striking increase of cytosolic cytochrome c. Caspase-8-specific inhibitor, z-ITED-fmk, canceled the cytotoxicity of fucoidan, activation of caspase-7, -8, and -9, and a series of changes in Bax, Bid, and cytochrome c. However, caspase-9-specific inhibitor exerted a moderate inhibitory effect on the cytotoxicity of fucoidan. These data indicated that fucoidan could induce apoptotic cell death through a caspase-8-dependent pathway in MCF-7 cells.
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Fucoidans induce apoptosis of HT-29, HCT116, HCT-15 colon cancer cells, MCF-7, SK-Mel-28, T-47D, and human promyeloid leukemic cell lines.
“Fucoidans were shown to induce apoptosis of some other cancer cells, for example HT-29, HCT116, and HCT-15 human colon cancer cells [42,43] as well as MCF-7 (breast adenocarcinoma) [44], melanoma SK-Mel-28, breast cancer T-47D [45], and human promyeloid leukemic cell lines [46].”
From Anticancer and Cancer Preventive Properties of Marine Polysaccharides: Some Results and Prospects (2013) · cites this paper as [44]
Fucoidan induces apoptosis in HT-29 colon cancer cells, MCF-7 human breast cancer cells, and HS-Sultan human lymphoma cells.
“Fucoidan was also reported to induce apoptosis in HT-29 colon cancer cells, 47 MCF-7 human breast cancer cells, 48 and HS-Sultan human lymphoma cells.49”
From Fucoidans from brown seaweeds: an update on structures, extraction techniques and use of enzymes as tools for structural elucidation (2013) · cites this paper as [48]
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- Authors
- Yamasaki-Miyamoto, Y., Yamasaki, M., Tachibana, H., & Yamada, K.
- Affiliations
- Kyushu University (JP)
- University of Miyazaki (JP)
- Corresponding author
- Yamasaki-Miyamoto, Yumi
- Venue
- Journal of Agricultural and Food Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2009-09-23
- Volume
- 57
- Issue
- 18
- Pages
- 8677-8682
- ISSN
- 0021-8561 (ISSN-L)
- 1520-5118
- OpenAlex
- W2052895145
- Cited by
- 181
- References
- 1
- Citations by year
- 2026: 6 · 2025: 7 · 2024: 4 · 2023: 11 · 2022: 7 · 2021: 15 · 2020: 12 · 2019: 24 · 2018: 6 · 2017: 8 · 2016: 7 · 2015: 12 · 2014: 19 · 2013: 16 · 2012: 10
- FWCI
- 13.59
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Agricultural and Food Chemistry (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Seaweed-derived Bioactive Compounds
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Cell death mechanisms and regulation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Natural product bioactivities and synthesis
- Keywords
- fucoidan, apoptosis, caspase-8, breast cancer, MCF-7 cells, Bid, Bax, cytochrome c, caspase-9, caspase-7, poly(ADP-ribose) polymerase cleavage, DNA fragmentation, z-VAD-fmk, seaweed polysaccharides, anticancer activity
- MeSH terms
- Antineoplastic Agents, Breast Neoplasms, Enzyme Activation, Enzyme Inhibitors, Humans, Poly(ADP-ribose) Polymerases, Polysaccharides, Apoptosis, Cytochromes c, Cell Line, Tumor, Caspase 8, Caspase Inhibitors
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