Fucoidan present in brown algae induces apoptosis of human colon cancer cells
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BACKGROUND: Fucoidan is a sulfated polysaccharide found in brown algae; it has been shown to exhibit a number of biological effects, including anti-tumor effects. In this study, we evaluated the effects of fucoidan on apoptosis in HT-29 and HCT116 human colon cancer cells. METHODS: HT-29 and HCT116 cells were cultured with various concentrations of fucoidan (0 - 20 microg/mL). Apoptosis was assayed via Hoechst staining and Annexin V staining followed by flow cytometric analysis. Western blot analyses and JC-1 staining were conducted to determine the levels of apoptosis-regulating proteins and mitochondrial membrane permeability, respectively. RESULTS: Fucoidan induced substantial reductions in viable cell numbers and apoptosis of HT-29 and HCT116 cells in a dose-dependent manner. In HT-29 cells, fucoidan also increased the levels of cleaved caspases-8, -9, -7, and -3, and cleaved poly (ADP-ribose) polymerase (PARP) levels. The levels of the X-linked inhibitor of apoptosis protein and survivin were attenuated in the fucoidan-treated cells. Fucoidan was also shown to enhance mitochondrial membrane permeability, as well as the cytochrome c and Smac/Diablo release from the mitochondria. Fucoidan increased the levels of the Bak and truncated Bid proteins, but reduced the levels of Mcl-1. Additionally, fucoidan increased the levels of the tumor necrosis factor-related apoptosis-inducing ligand, Fas and death receptor 5 proteins. The caspase-8 and -9 inhibitors Z-IETD-FMK and Z-LEHD-FMK induced a reduction in fucoidan-mediated apoptosis. Caspase-8 inhibitor inhibited the fucoidan-induced cleavage of Bid, caspases-9 and -3, and PARP. CONCLUSION: The findings of this study indicate that fucoidan induces apoptosis in HT-29 and HCT116 human colon cancer cells, and that this phenomenon is mediated via both the death receptor-mediated and mitochondria-mediated apoptotic pathways. These results suggest that fucoidan may prove useful in the development of a colon cancer-preventive protocol.
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Fucoidans induce apoptosis of HT-29, HCT116, HCT-15 colon cancer cells, MCF-7, SK-Mel-28, T-47D, and human promyeloid leukemic cell lines.
“Fucoidans were shown to induce apoptosis of some other cancer cells, for example HT-29, HCT116, and HCT-15 human colon cancer cells [42,43] as well as MCF-7 (breast adenocarcinoma) [44], melanoma SK-Mel-28, breast cancer T-47D [45], and human promyeloid leukemic cell lines [46].”
From Anticancer and Cancer Preventive Properties of Marine Polysaccharides: Some Results and Prospects (2013) · cites this paper as [43]
Fucoidan induces apoptosis in HT-29 colon cancer cells, MCF-7 human breast cancer cells, and HS-Sultan human lymphoma cells.
“Fucoidan was also reported to induce apoptosis in HT-29 colon cancer cells, 47 MCF-7 human breast cancer cells, 48 and HS-Sultan human lymphoma cells.49”
From Fucoidans from brown seaweeds: an update on structures, extraction techniques and use of enzymes as tools for structural elucidation (2013) · cites this paper as [47]
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- Authors
- Kim, E. J., Park, S. Y., Lee, J.-Y., & Park, J. H. Y.
- Affiliations
- Hallym University (KR)
- Corresponding author
- Park, Jung Han Yoon
- Venue
- BMC Gastroenterology
- Published
- 2010-08-22
- Volume
- 10
- Issue
- 1
- Pages
- 96-96
- ISSN
- 1471-230X (ISSN-L)
- PMC
- PMC2931458
- OpenAlex
- W2160738153
- Cited by
- 275
- References
- 50
- Citations by year
- 2026: 9 · 2025: 7 · 2024: 14 · 2023: 18 · 2022: 25 · 2021: 20 · 2020: 19 · 2019: 24 · 2018: 15 · 2017: 20 · 2016: 17 · 2015: 23 · 2014: 18 · 2013: 15 · 2012: 14
- FWCI
- 22.52
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- BMC Gastroenterology (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- National Research Foundation
- Hallym University
- Ministry of Knowledge Economy
- National Research Foundation of Korea
- Ministry of Education, Science and Technology
- Topics
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Seaweed-derived Bioactive Compounds
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Cell death mechanisms and regulation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Natural product bioactivities and synthesis
- Keywords
- fucoidan, brown algae, apoptosis, colon cancer, HT-29 cells, HCT116 cells, caspase activation, mitochondrial membrane permeability, cytochrome c release, Smac/DIABLO, Bak, Bid, Mcl-1, Fas, DR5, death receptor-mediated apoptosis, mitochondrial apoptosis, colon cancer prevention
- MeSH terms
- Adenocarcinoma, Phaeophyceae, Antineoplastic Agents, Cell Membrane Permeability, Cell Survival, Colonic Neoplasms, Humans, Phytotherapy, Plant Extracts, Polysaccharides, Apoptosis, HT29 Cells, Proto-Oncogene Proteins c-bcl-2, Cytochromes c, Cell Line, Tumor, Cell Proliferation
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