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The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.

In Vitro Antioxidant and ex Vivo Protective Activities of Green and Roasted Coffee

Daglia, Maria, Papetti, Adele, Gregotti, Cesarina, Bertè, Francantonio, Gazzani, Gabriella·2000·Journal of Agricultural and Food Chemistry

Abstract

The antioxidant properties of green and roasted coffee, in relation to species (Coffea arabica and Coffea robusta) and degree of roasting (light, medium, dark), were investigated. These properties were evaluated by determining the reducing substances (RS) of coffee and its antioxidant activity (AA) in vitro (model system beta-carotene-linoleic acid) and ex vivo as protective activity (PA) against rat liver cell microsome lipid peroxidation measured as TBA-reacting substances. RS of C. robustasamples were found to be significantly higher when compared to those of C. arabica samples (p < 0.001). AA for green coffee samples were slightly higher than for the corresponding roasted samples while PA was significantly lower in green coffee compared to that of all roasted samples (p < 0.001). Extraction with three different organic solvents (ethyl acetate, ethyl ether, and dichloromethane) showed that the most protective compounds are extracted from acidified dark roasted coffee solutions with ethyl acetate. The analysis of acidic extract by gel filtration chromatography (GFC) gave five fractions. Higher molecular mass fractions were found to possess antioxidant activity while the lower molecular mass fractions showed protective activity. The small amounts of these acidic, low molecular mass protective fractions isolated indicate that they contain very strong protective agents.

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Metadata

Authors
Daglia, M., Papetti, A., Gregotti, C., Bertè, F., & Gazzani, G.
Venue
Journal of Agricultural and Food Chemistry
Published
2000-04-18
Volume
48
Issue
5
Pages
1449-1454
ISSN
  • 0021-8561 (ISSN-L)
  • 1520-5118
OpenAlex
W2286829829
Cited by
288
References
3
FWCI
3.92
Type
journal article
Language
en
Open access
closed

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