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Novel Polyion Complex Micelles Entrapping Enzyme Molecules in the Core: Preparation of Narrowly-Distributed Micelles from Lysozyme and Poly(ethylene glycol)−Poly(aspartic acid) Block Copolymer in Aqueous Medium
Abstract
A core−shell-type supramolecular assembly, a polyion complex micelle, was prepared in this study from chicken egg white lysozyme and poly(ethylene glycol)−poly(aspartic acid) block copolymer (PEG-P(Asp)) through electrostatic interaction in aqueous medium. Lysozyme/PEG-P(Asp) micelles thus prepared had an extremely narrow distribution (μ 2 /Γ 2 < 0.04) with an average diameter of 47 nm in dynamic light scattering measurements. No precipitate formation was observed even after 1 month standing at ambient temperature, suggesting that the system is in a thermodynamic equilibrium state. The stoichiometry in terms of the molar ratio of Lys and Arg residues in lysozyme and Asp residues in PEG-P(Asp) was confirmed by dynamic and static light scattering as well as by laser-Doppler electrophoresis measurements. A change in the apparent molar mass of the micelle with varying PEG-P(Asp)/lysozyme ratio in the region with excess lysozyme agreed well with calculated values if a cooperative association mechanism is assumed to occur. The diffusion coefficient of lysozyme/PEG-P(Asp) micelles prepared at a stoichiometric mixing ratio showed neither angular nor concentration dependence, indicating their spherical shape with no secondary aggregate formation. A core−shell structure with a polyion complex core and a PEG corona was suggested from an extremely low absolute value of zeta-potential. The association numbers of lysozyme and PEG-P(Asp) in the stoichiometric micelle were calculated from the apparent molar mass and were determined to be 36 and 42 for lysozyme and PEG-P(Asp), respectively. Such PIC micelles entrapping enzymes in the core are expected to be useful as functional materials including carrier systems in drug delivery applications and a nanometric-scale reactor for enzymes.
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Kwon, G., Suwa, S., Yokoyama, M., Okano, T., Sakurai, Y., & Kataoka, K. (1994) · Journal of Controlled Release · doi:10.1016/0168-3659(94)90118-x · Cited by 409
Self-citationMicelles based on AB block copolymers of poly (ethylene oxide) and poly (beta.-benzyl L-aspartate)
Kwon, G., Naito, M., Yokoyama, M., Okano, T., Sakurai, Y., & Kataoka, K. (1993) · Langmuir · doi:10.1021/la00028a012 · Cited by 399
Self-citationKabanov, A. V., Chekhonin, V. P., Alakhov, V. Y., Batrakova, E. V., Lebedev, A. S., Melik-Nubarov, N. S., Arzhakov, S. A., Levashov, A. V., Morozov, G. V., Severin, E. S., et al. (1989) · FEBS Letters · doi:10.1016/0014-5793(89)81689-8 · Cited by 296
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Bae, Y., Fukushima, S., Harada, A., & Kataoka, K. (2003) · Angewandte Chemie International Edition · doi:10.1002/anie.200250653 · Cited by 1,329
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Lee, Y., Ishii, T., Cabral, H., Kim, H. J., Seo, J., Nishiyama, N., Oshima, H., Osada, K., & Kataoka, K. (2009) · Angewandte Chemie International Edition · doi:10.1002/anie.200900064 · Cited by 351
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Metadata
- Authors
- Harada, A., & Kataoka, K.
- Venue
- Macromolecules
- Publisher
- American Chemical Society (ACS)
- Published
- 1998-01-01
- Volume
- 31
- Issue
- 2
- Pages
- 288-294
- ISSN
- 0024-9297 (ISSN-L)
- 1520-5835
- OpenAlex
- W2065422398
- Cited by
- 482
- References
- 3
- FWCI
- 13.96
- Type
- journal article
- Language
- en
- Open access
- closed
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