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Cited by 263

The DOI matches its Crossref record; 45 of the 45 references it cites were found in a registry or an index.

Nanolithographic Control of the Spatial Organization of Cellular Adhesion Receptors at the Single-Molecule Level

Open access
Schvartzman, Mark, Palma, Matteo, Sable, Julia, Abramson, Justin, Hu, Xian, Sheetz, Michael P., Wind, Shalom J.·2011·Nano Letters
DOI 10.1021/nl104378f
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Abstract

The ability to control the placement of individual molecules promises to enable a wide range of applications and is a key challenge in nanoscience and nanotechnology. Many biological interactions, in particular, are sensitive to the precise geometric arrangement of proteins. We have developed a technique which combines molecular-scale nanolithography with site-selective biochemistry to create biomimetic arrays of individual protein binding sites. The binding sites can be arranged in heterogeneous patterns of virtually any possible geometry with a nearly unlimited number of degrees of freedom. We have used these arrays to explore how the geometric organization of the extracellular matrix (ECM) binding ligand RGD (Arg-Gly-Asp) affects cell adhesion and spreading. Systematic variation of spacing, density, and cluster size of individual integrin binding sites was used to elicit different cell behavior. Cell spreading assays on arrays of different geometric arrangements revealed a dramatic increase in spreading efficiency when at least four liganded sites were spaced within 60 nm or less, with no dependence on global density. This points to the existence of a minimal matrix adhesion unit for fibronectin defined in space and stoichiometry. Developing an understanding of the ECM geometries that activate specific cellular functional complexes is a critical step toward controlling cell behavior. Potential practical applications range from new therapeutic treatments to the rational design of tissue scaffolds that can optimize healing without scarring. More broadly, spatial control at the single-molecule level can elucidate factors controlling individual molecular interactions and can enable synthesis of new systems based on molecular-scale architectures.

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Metadata

Authors
Schvartzman, M., Palma, M., Sable, J., Abramson, J., Hu, X., Sheetz, M. P., & Wind, S. J.
Venue
Nano Letters
Publisher
American Chemical Society (ACS)
Published
2011-02-14
Volume
11
Issue
3
Pages
1306-1312
ISSN
  • 1530-6984 (ISSN-L)
  • 1530-6992
DOI
10.1021/nl104378f
OpenAlex
W2077465579
Cited by
263
References
45
FWCI
8.31
Type
journal article
Language
en
Open access
green

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