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Effects of Cell Culture Media on the Dynamic Formation of Protein−Nanoparticle Complexes and Influence on the Cellular Response

Maiorano, Gabriele, Sabella, Stefania, Sorce, Barbara, Brunetti, Virgilio, Malvindi, Maria Ada, Cingolani, Roberto, Pompa, Pier Paolo·2010·ACS Nano
DOI 10.1021/nn101557e
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Abstract

The development of appropriate in vitro protocols to assess the potential toxicity of the ever expanding range of nanoparticles represents a challenging issue, because of the rapid changes of their intrinsic physicochemical properties (size, shape, reactivity, surface area, etc.) upon dispersion in biological fluids. Dynamic formation of protein coating around nanoparticles is a key molecular event, which may strongly impact the biological response in nanotoxicological tests. In this work, by using citrate-capped gold nanoparticles (AuNPs) of different sizes as a model, we show, by several spectroscopic techniques (dynamic light scattering, UV-visible, plasmon resonance light scattering), that proteins-NP interactions are differently mediated by two widely used cellular media (i.e., Dulbecco Modified Eagle's medium (DMEM) and Roswell Park Memorial Institute medium (RPMI), supplemented with fetal bovine serum). We found that, while DMEM elicits the formation of a large time-dependent protein corona, RPMI shows different dynamics with reduced protein coating. Characterization of these nanobioentities was also performed by sodium dodecyl sulfate polyacrylamide gel electrophoresis and mass spectroscopy, revealing that the average composition of protein corona does not reflect the relative abundance of serum proteins. To evaluate the biological impact of such hybrid bionanostructures, several comparative viability assays onto two cell lines (HeLa and U937) were carried out in the…

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Metadata

Authors
Maiorano, G., Sabella, S., Sorce, B., Brunetti, V., Malvindi, M. A., Cingolani, R., & Pompa, P. P.
Venue
ACS Nano
Publisher
American Chemical Society (ACS)
Published
2010-11-17
Volume
4
Issue
12
Pages
7481-7491
ISSN
  • 1936-0851
  • 1936-086X
DOI
10.1021/nn101557e
Cited by
573
References
1
Language
en

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