CoChat
Sign Up
Cited by 624

The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.

Cationic Polystyrene Nanosphere Toxicity Depends on Cell-Specific Endocytic and Mitochondrial Injury Pathways

Xia, Tian, Kovochich, Michael, Liong, Monty, Zink, Jeffrey I., Nel, Andre E.·2008·ACS Nano
DOI 10.1021/nn700256c
Open this paper in CoChat — free account

Abstract

The exponential increase in the number of new nanomaterials that are being produced increases the likelihood of adverse biological effects in humans and the environment. In this study we compared the effects of cationic nanoparticles in five different cell lines that represent portal-of-entry or systemic cellular targets for engineered nanoparticles. Although 60 nm NH(2)-labeled polystyrene (PS) nanospheres were highly toxic in macrophage (RAW 264.7) and epithelial (BEAS-2B) cells, human microvascular endothelial (HMEC), hepatoma (HEPA-1), and pheochromocytoma (PC-12) cells were relatively resistant to particle injury. While the death pathway in RAW 264.7 cells involves caspase activation, the cytotoxic response in BEAS-2B cells is more necrotic in nature. Using fluorescent-labeled NH(2)-PS, we followed the routes of particle uptake. Confocal microscopy showed that the cationic particles entered a LAMP-1 positive lysosomal compartment in RAW 264.7 cells from where the particles could escape by lysosomal rupture. A proton pump inhibitor interfered in this pathway. Subsequent deposition of the particles in the cytosol induced an increase in mitochondrial Ca(2+) uptake and cell death that could be suppressed by cyclosporin A (CsA). In contrast, NH(2)-PS toxicity in BEAS-2B cells did not involve the LAMP-1 endosomal compartment, stimulation of proton pump activity, or an increase in mitochondrial Ca(2+). Particles were taken up by caveolae, and their toxicity could be disrupted b…

Overview

Integrity signals

Registry

Crossref · 10.1021/nn700256c

Cites

2 of 2 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Verification

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Citations

Cites

Show all 2 references

Research works printed but not linked: 46

  1. Nel A., 2006, Science, 311, 622, 10.1126/science.1114397 · DOI · Paper page
  2. Colvin V. L., 2003, Nat. Biotechnol., 21, 1166, 10.1038/nbt875 · DOI
  3. Donaldson K., 2004, Occup. Environ. Med, 61, 727, 10.1136/oem.2004.013243 · DOI
  4. Oberdorster G., 2005, Environ. Health Perspect., 113, 823, 10.1289/ehp.7339 · DOI · Paper page
  5. Service R. F., 2004, Science, 304, 1732, 10.1126/science.304.5678.1732 · DOI
  6. Linse S., 2007, Proc. Natl. Acad. Sci. U.S.A., 104, 8691, 10.1073/pnas.0701250104 · DOI
  7. Oberdorster G., 2005, Part. Fibre Toxicol., 2, 8, 10.1186/1743-8977-2-8 · DOI · Paper page
  8. Xia T., 2006, Nano Lett., 6, 1794, 10.1021/nl061025k · DOI
  9. Hoet P. H., 2001, Toxicol. Appl. Pharmacol., 175, 184, 10.1006/taap.2001.9247 · DOI
  10. Nemmar A., 2003, Toxicol. Appl. Pharmacol., 186, 38, 10.1016/S0041-008X(02)00024-8 · DOI
  11. Chen C. Z., 2002, Biomaterials, 23, 3359, 10.1016/S0142-9612(02)00036-4 · DOI
  12. Boussif O., 1995, Proc. Natl. Acad. Sci. U.S.A., 92, 7297, 10.1073/pnas.92.16.7297 · DOI
  13. Sonawane N. D., 2002, J. Biol. Chem., 277, 5506, 10.1074/jbc.M110818200 · DOI
  14. Asokan A., 2002, J. Pharm. Sci., 91, 903, 10.1002/jps.10095 · DOI
  15. Hug H., 1999, Biochemistry, 38, 13906, 10.1021/bi9913395 · DOI
  16. Kirkham M., 2005, Biochim. Biophys. Acta, 1746, 349, 10.1016/j.bbamcr.2005.11.005 · DOI
  17. Sieczkarski S. B., 2002, J. Gen. Virol., 83, 1535, 10.1099/0022-1317-83-7-1535 · DOI
  18. Halestrap A. P., 2002, Biochimie, 84, 153, 10.1016/S0300-9084(02)01375-5 · DOI · Paper page
  19. Kim J. S., 2003, Biochem. Biophys. Res. Commun., 304, 463, 10.1016/S0006-291X(03)00618-1 · DOI
  20. Hao M., 2003, J. Allergy Clin. Immunol., 112, 905, 10.1016/j.jaci.2003.07.005 · DOI
  21. Polo S., 2006, Cell, 124, 897, 10.1016/j.cell.2006.02.025 · DOI
  22. Hong S., 2006, Bioconjugate Chem., 17, 728, 10.1021/bc060077y · DOI
  23. Reiners J. J., 2002, Cell Death Differ., 9, 934, 10.1038/sj.cdd.4401048 · DOI
  24. Terman A., 2006, IUBMB Life, 58, 531, 10.1080/15216540600904885 · DOI
  25. Terman A., 2006, Chem. Biol. Interact., 163, 29, 10.1016/j.cbi.2006.04.013 · DOI
  26. Szabadkai G., 2006, Biochim. Biophys. Acta, 1763, 442, 10.1016/j.bbamcr.2006.04.002 · DOI
  27. Rizzuto R., 2006, Physiol. Rev., 86, 369, 10.1152/physrev.00004.2005 · DOI
  28. Drab M., 2001, Science, 293, 2449, 10.1126/science.1062688 · DOI
  29. Peterson T. E., 2003, Arterioscler. Thromb. Vasc. Biol., 23, 1521, 10.1161/01.ATV.0000081743.35125.05 · DOI
  30. Goetz J. G., 2006, Biochem. Soc. Trans., 34, 370, 10.1042/BST0340370 · DOI
  31. Jiang Y. N., 2007, Cancer Cell Int., 7, 1, 10.1186/1475-2867-7-1 · DOI
  32. Daniel E. E., 2006, J. Cell Mol. Med., 10, 529, 10.1111/j.1582-4934.2006.tb00418.x · DOI
  33. Plotkowski M. C., 2002, Am. J. Respir. Cell Mol. Biol., 26, 617, 10.1165/ajrcmb.26.5.4489 · DOI
  34. Papadimitriou J. C., 1994, Virchows Arch., 424, 677, 10.1007/BF00195784 · DOI
  35. Akinc A., 2005, J. Gene Med., 7, 657, 10.1002/jgm.696 · DOI
  36. van der Aa M. A., 2007, Pharm. Res., 24, 1590, 10.1007/s11095-007-9287-3 · DOI
  37. von G. K., 2006, Mol. Ther., 14, 745, 10.1016/j.ymthe.2006.07.006 · DOI
  38. Rejman J., 2005, Mol. Ther., 12, 468, 10.1016/j.ymthe.2005.03.038 · DOI
  39. Florea B. I., 2002, AAPS Pharm. Sci., 4, E12, 10.1208/ps040312 · DOI
  40. Wiepz G. J., 2006, Methods Mol. Biol., 327, 179
  41. Hiura T. S., 2000, J. Immunol., 165, 2703, 10.4049/jimmunol.165.5.2703 · DOI
  42. Li N., 2000, J. Immunol., 165, 3393, 10.4049/jimmunol.165.6.3393 · DOI · Paper page
  43. Qhobosheane M., 2001, Analyst, 126, 1274, 10.1039/b101489g · DOI
  44. Udenfriend S., 1972, Science, 178, 871, 10.1126/science.178.4063.871 · DOI
  45. Zhou M., 2006, J. Neurophysiol., 95, 134, 10.1152/jn.00570.2005 · DOI
  46. Yuan X. M., 2002, Proc. Natl. Acad. Sci. U.S.A., 99, 6286, 10.1073/pnas.092135599 · DOI

Cited by

Sorted by citations

Metadata

Authors
Xia, T., Kovochich, M., Liong, M., Zink, J. I., & Nel, A. E.
Affiliations
  • Division of NanoMedicine, Department of Medicine
  • Department of Chemistry & Biochemistry
Venue
ACS Nano
Publisher
American Chemical Society (ACS)
Published
2007-12-27
Volume
2
Issue
1
Pages
85-96
ISSN
  • 1936-0851
  • 1936-086X
DOI
10.1021/nn700256c
Cited by
624
References
2
Language
en

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review