The DOI matches its Crossref record; 43 of the 43 references it cites were found in a registry or an index.
CuO Nanoparticle Interaction with Human Epithelial Cells: Cellular Uptake, Location, Export, and Genotoxicity
Abstract
The toxicity of CuO nanoparticles (NPs) to human lung epithelial (A549) cells was investigated in this study. CuO NPs (10-100 mg/L) had significant toxicity to A549 cells, whereas CuO bulk particles (BPs) showed much lower toxicity (24 h IC(50), 58 and 15 mg/L for CuO BPs and NPs, respectively). Transmission electron microscopic analysis demonstrated CuO NP entry into A549 cells and organelles, including lysosomes, mitochondria, and nucleus. Endocytosis was the primary pathway of CuO NPs uptake. CuO NPs (15 mg/L) induced mitochondrial depolarization, possibly mediated by reactive oxygen species (ROS) generation. Intracellular CuO NPs first generate ROS, which subsequently induces the expression of p38 and p53 and ultimately causes DNA damage (Comet assay). We confirm for the first time that the primary cytotoxic response is oxidative stress rather than DNA damage. A fraction of the CuO NPs was exported to the extracellular environment. In this study, centrifugal ultrafiltration tubes were successfully employed to determine the dissolved Cu(2+) from CuO NPs in the cell medium. Dissolved Cu(2+) ions contributed less than half of the total toxicity caused by CuO NPs, including ROS generation and DNA damage. This study provided useful data for understanding transport and toxicity of metal oxide NPs in human cells.
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Cites
Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT Method
Livak, K. J., & Schmittgen, T. D. (2001) · Methods · doi:10.1006/meth.2001.1262 · Cited by 180,860
A simple technique for quantitation of low levels of DNA damage in individual cells
Singh, N. P., McCoy, M. T., Tice, R. R., & Schneider, E. L. (1988) · Experimental Cell Research · doi:10.1016/0014-4827(88)90265-0 · Cited by 10,828
Cytotoxicity and Genotoxicity of Silver Nanoparticles in Human Cells
AshaRani, P. V., Low Kah Mun, G., Hande, M. P., & Valiyaveettil, S. (2009) · ACS Nano · doi:10.1021/nn800596w · Cited by 3,692
Xia, T., Kovochich, M., Liong, M., Mädler, L., Gilbert, B., Shi, H., Yeh, J. I., Zink, J. I., & Nel, A. E. (2008) · ACS Nano · doi:10.1021/nn800511k · Cited by 2,469
Chithrani, B. D., & Chan, W. C. W. (2007) · Nano Letters · doi:10.1021/nl070363y · Cited by 2,286
Xia, T., Kovochich, M., Brant, J., Hotze, M., Sempf, J., Oberley, T., Sioutas, C., Yeh, J. I., Wiesner, M. R., & Nel, A. E. (2006) · Nano Letters · doi:10.1021/nl061025k · Cited by 1,918
Karlsson, H. L., Cronholm, P., Gustafsson, J., & Möller, L. (2008) · Chemical Research in Toxicology · doi:10.1021/tx800064j · Cited by 1,480
Toxicity of Silver Nanoparticles to Chlamydomonas reinhardtii
Navarro, E., Piccapietra, F., Wagner, B., Marconi, F., Kaegi, R., Odzak, N., Sigg, L., & Behra, R. (2008) · Environmental Science & Technology · doi:10.1021/es801785m · Cited by 1,457
Geiser, M., Rothen-Rutishauser, B., Kapp, N., Schürch, S., Kreyling, W., Schulz, H., Semmler, M., Hof, V. I., Heyder, J., & Gehr, P. (2005) · Environmental Health Perspectives · doi:10.1289/ehp.8006 · Cited by 1,407
Yang, H., Liu, C., Yang, D., Zhang, H., & Xi, Z. (2009) · Journal of Applied Toxicology · doi:10.1002/jat.1385 · Cited by 1,087
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Behzadi, S., Serpooshan, V., Tao, W., Hamaly, M. A., Alkawareek, M. Y., Dreaden, E. C., Brown, D., Alkilany, A. M., Farokhzad, O. C., & Mahmoudi, M. (2017) · Chemical Society Reviews · doi:10.1039/c6cs00636a · Cited by 2,859
The Toxic Effects and Mechanisms of CuO and ZnO Nanoparticles
Chang, Y.-N., Zhang, M., Xia, L., Zhang, J., & Xing, G. (2012) · Materials · doi:10.3390/ma5122850 · Cited by 772
Reactive Oxygen Species-Related Nanoparticle Toxicity in the Biomedical Field
Yu, Z., Li, Q., Wang, J., Yu, Y., Wang, Y., Zhou, Q., & Li, P. (2020) · Nanoscale Research Letters · doi:10.1186/s11671-020-03344-7 · Cited by 709
Antimicrobial Nano-Agents: The Copper Age
Ermini, M. L., & Voliani, V. (2021) · ACS Nano · doi:10.1021/acsnano.0c10756 · Cited by 477
Toxicity of copper oxide nanoparticles: a review study
Naz, S., Gul, A., & Zia, M. (2020) · IET Nanobiotechnology · doi:10.1049/iet-nbt.2019.0176 · Cited by 447
Antimicrobial applications of copper
Vincent, M., Hartemann, P., & Engels-Deutsch, M. (2016) · International Journal of Hygiene and Environmental Health · doi:10.1016/j.ijheh.2016.06.003 · Cited by 384
Ivask, A., Juganson, K., Bondarenko, O., Mortimer, M., Aruoja, V., Kasemets, K., Blinova, I., Heinlaan, M., Slaveykova, V., & Kahru, A. (2014) · Nanotoxicology · doi:10.3109/17435390.2013.855831 · Cited by 332
Ma, X., Zhou, S., Xu, X., & Du, Q. (2022) · Frontiers in Surgery · doi:10.3389/fsurg.2022.905892 · Cited by 317
Karlsson, H. L., Cronholm, P., Hedberg, Y., Tornberg, M., De Battice, L., Svedhem, S., & Wallinder, I. O. (2013) · Toxicology · doi:10.1016/j.tox.2013.07.012 · Cited by 286
Size-dependent cellular uptake and localization profiles of silver nanoparticles
Wu, M., Guo, H., Liu, L., Liu, Y., & Xie, L. (2019) · International Journal of Nanomedicine · doi:10.2147/ijn.s201107 · Cited by 282
Synthesis, biomedical applications, and toxicity of CuO nanoparticles
Naz, S., Gul, A., Zia, M., & Javed, R. (2023) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-023-12364-z · Cited by 279
Biofabrication of nanoparticles: sources, synthesis, and biomedical applications
Kulkarni, D., Sherkar, R., Shirsathe, C., Sonwane, R., Varpe, N., Shelke, S., More, M. P., Pardeshi, S. R., Dhaneshwar, G., Junnuthula, V., et al. (2023) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2023.1159193 · Cited by 267
Semisch, A., Ohle, J., Witt, B., & Hartwig, A. (2014) · Particle and Fibre Toxicology · doi:10.1186/1743-8977-11-10 · Cited by 203
Yang, C., Uertz, J., Yohan, D., & Chithrani, B. D. (2014) · Nanoscale · doi:10.1039/c4nr02535k · Cited by 156
Toxic mechanisms of copper oxide nanoparticles in epithelial kidney cells
Thit, A., Selck, H., & Bjerregaard, H. F. (2015) · Toxicology in Vitro · doi:10.1016/j.tiv.2015.03.020 · Cited by 85
Metadata
- Authors
- Wang, Z., Li, N., Zhao, J., White, J. C., Qu, P., & Xing, B.
- Venue
- Chemical Research in Toxicology
- Publisher
- American Chemical Society (ACS)
- Published
- 2012-06-27
- Volume
- 25
- Issue
- 7
- Pages
- 1512-1521
- ISSN
- 0893-228X (ISSN-L)
- 1520-5010
- OpenAlex
- W2324506251
- Cited by
- 340
- References
- 43
- FWCI
- 11.00
- Type
- journal article
- Language
- en
- Open access
- closed
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