The DOI matches its Crossref record; 43 of the 43 references it cites were found in a registry or an index.
CuO Nanoparticle Interaction with Human Epithelial Cells: Cellular Uptake, Location, Export, and Genotoxicity
Abstract
The toxicity of CuO nanoparticles (NPs) to human lung epithelial (A549) cells was investigated in this study. CuO NPs (10-100 mg/L) had significant toxicity to A549 cells, whereas CuO bulk particles (BPs) showed much lower toxicity (24 h IC(50), 58 and 15 mg/L for CuO BPs and NPs, respectively). Transmission electron microscopic analysis demonstrated CuO NP entry into A549 cells and organelles, including lysosomes, mitochondria, and nucleus. Endocytosis was the primary pathway of CuO NPs uptake. CuO NPs (15 mg/L) induced mitochondrial depolarization, possibly mediated by reactive oxygen species (ROS) generation. Intracellular CuO NPs first generate ROS, which subsequently induces the expression of p38 and p53 and ultimately causes DNA damage (Comet assay). We confirm for the first time that the primary cytotoxic response is oxidative stress rather than DNA damage. A fraction of the CuO NPs was exported to the extracellular environment. In this study, centrifugal ultrafiltration tubes were successfully employed to determine the dissolved Cu(2+) from CuO NPs in the cell medium. Dissolved Cu(2+) ions contributed less than half of the total toxicity caused by CuO NPs, including ROS generation and DNA damage. This study provided useful data for understanding transport and toxicity of metal oxide NPs in human cells.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT Method
Livak, K. J., & Schmittgen, T. D. (2001) · Methods · doi:10.1006/meth.2001.1262 · Cited by 180,860
A simple technique for quantitation of low levels of DNA damage in individual cells
Singh, N. P., McCoy, M. T., Tice, R. R., & Schneider, E. L. (1988) · Experimental Cell Research · doi:10.1016/0014-4827(88)90265-0 · Cited by 10,828
Cytotoxicity and Genotoxicity of Silver Nanoparticles in Human Cells
AshaRani, P. V., Low Kah Mun, G., Hande, M. P., & Valiyaveettil, S. (2009) · ACS Nano · doi:10.1021/nn800596w · Cited by 3,692
Xia, T., Kovochich, M., Liong, M., Mädler, L., Gilbert, B., Shi, H., Yeh, J. I., Zink, J. I., & Nel, A. E. (2008) · ACS Nano · doi:10.1021/nn800511k · Cited by 2,469
Chithrani, B. D., & Chan, W. C. W. (2007) · Nano Letters · doi:10.1021/nl070363y · Cited by 2,286
Xia, T., Kovochich, M., Brant, J., Hotze, M., Sempf, J., Oberley, T., Sioutas, C., Yeh, J. I., Wiesner, M. R., & Nel, A. E. (2006) · Nano Letters · doi:10.1021/nl061025k · Cited by 1,918
Karlsson, H. L., Cronholm, P., Gustafsson, J., & Möller, L. (2008) · Chemical Research in Toxicology · doi:10.1021/tx800064j · Cited by 1,480
Toxicity of Silver Nanoparticles to Chlamydomonas reinhardtii
Navarro, E., Piccapietra, F., Wagner, B., Marconi, F., Kaegi, R., Odzak, N., Sigg, L., & Behra, R. (2008) · Environmental Science & Technology · doi:10.1021/es801785m · Cited by 1,457
Geiser, M., Rothen-Rutishauser, B., Kapp, N., Schürch, S., Kreyling, W., Schulz, H., Semmler, M., Hof, V. I., Heyder, J., & Gehr, P. (2005) · Environmental Health Perspectives · doi:10.1289/ehp.8006 · Cited by 1,407
Yang, H., Liu, C., Yang, D., Zhang, H., & Xi, Z. (2009) · Journal of Applied Toxicology · doi:10.1002/jat.1385 · Cited by 1,087
Size-dependent toxicity of metal oxide particles—A comparison between nano- and micrometer size
Karlsson, H. L., Gustafsson, J., Cronholm, P., & Möller, L. (2009) · Toxicology Letters · doi:10.1016/j.toxlet.2009.03.014 · Cited by 949
Transport into and out of the Nucleus
Macara, I. G. (2001) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.65.4.570-594.2001 · Cited by 905
Huang, M., Khor, E., & Lim, L.-Y. (2004) · Pharmaceutical Research · doi:10.1023/b:pham.0000016249.52831.a5 · Cited by 853
DNA damage response to different surface chemistry of silver nanoparticles in mammalian cells
Ahamed, M., Karns, M., Goodson, M., Rowe, J., Hussain, S. M., Schlager, J. J., & Hong, Y. (2008) · Toxicology and Applied Pharmacology · doi:10.1016/j.taap.2008.09.015 · Cited by 730
Inorganic and organic UV filters: Their role and efficacy in sunscreens and suncare products
Serpone, N., Dondi, D., & Albini, A. (2007) · Inorganica Chimica Acta · doi:10.1016/j.ica.2005.12.057 · Cited by 711
Surface-Charge-Dependent Cell Localization and Cytotoxicity of Cerium Oxide Nanoparticles
Asati, A., Santra, S., Kaittanis, C., & Perez, J. M. (2010) · ACS Nano · doi:10.1021/nn100816s · Cited by 701
DNA damaging potential of zinc oxide nanoparticles in human epidermal cells
Sharma, V., Shukla, R. K., Saxena, N., Parmar, D., Das, M., & Dhawan, A. (2009) · Toxicology Letters · doi:10.1016/j.toxlet.2009.01.008 · Cited by 633
Wang, L., Liu, Y., Li, W., Jiang, X., Ji, Y., Wu, X., Xu, L., Qiu, Y., Zhao, K., Wei, T., et al. (2011) · Nano Letters · doi:10.1021/nl103992v · Cited by 514
Giglia-Mari, G., Zotter, A., & Vermeulen, W. (2011) · Cold Spring Harbor Perspectives in Biology · doi:10.1101/cshperspect.a000745 · Cited by 472
Effects of rare earth oxide nanoparticles on root elongation of plants
Ma, Y., Kuang, L., He, X., Bai, W., Ding, Y., Zhang, Z., Zhao, Y., & Chai, Z. (2010) · Chemosphere · doi:10.1016/j.chemosphere.2009.10.050 · Cited by 466
Midander, K., Cronholm, P., Karlsson, H. L., Elihn, K., Möller, L., Leygraf, C., & Wallinder, I. O. (2009) · Small · doi:10.1002/smll.200801220 · Cited by 431
Akey, C., & Radermacher, M. (1993) · The Journal of cell biology · doi:10.1083/jcb.122.1.1 · Cited by 406
Eom, H.-J., & Choi, J. (2010) · Environmental Science & Technology · doi:10.1021/es1020668 · Cited by 357
Gunawan, C., Teoh, W. Y., Marquis, C. P., & Amal, R. (2011) · ACS Nano · doi:10.1021/nn2020248 · Cited by 349
Wang, Z., Li, J., Zhao, J., & Xing, B. (2011) · Environmental Science & Technology · doi:10.1021/es2010573 · Cited by 347
Self-citationSimon-Deckers, A., Gouget, B., Mayne-L’Hermite, M., Herlin-Boime, N., Reynaud, C., & Carrière, M. (2008) · Toxicology · doi:10.1016/j.tox.2008.09.007 · Cited by 324
Endo- and Exocytosis of Zwitterionic Quantum Dot Nanoparticles by Live HeLa Cells
Jiang, X., Röcker, C., Hafner, M., Brandholt, S., Dörlich, R. M., & Nienhaus, G. U. (2010) · ACS Nano · doi:10.1021/nn101277w · Cited by 299
pH-dependent toxicity of copper and uranium to a tropical freshwater alga (Chlorella sp.).
Franklin, N. M., Stauber, J. L., Markich, S. J., & Lim, R. P. (2000) · Aquatic Toxicology · doi:10.1016/s0166-445x(99)00042-9 · Cited by 273
Ultrahigh reactivity provokes nanotoxicity: Explanation of oral toxicity of nano-copper particles
MENG, H., CHEN, Z., XING, G., YUAN, H., CHEN, C., ZHAO, F., ZHANG, C., & ZHAO, Y. (2007) · Toxicology Letters · doi:10.1016/j.toxlet.2007.09.015 · Cited by 262
Nuclear pores and nuclear assembly
Vasu, S. K., & Forbes, D. J. (2001) · Current Opinion in Cell Biology · doi:10.1016/s0955-0674(00)00221-0 · Cited by 254
Tan, C., Lai, S., Wu, S., Hu, S., Zhou, L., Chen, Y., Wang, M., Zhu, Y., Lian, W., Peng, W., et al. (2010) · Journal of Medicinal Chemistry · doi:10.1021/jm1009296 · Cited by 244
Mu, Q., Broughton, D. L., & Yan, B. (2009) · Nano Letters · doi:10.1021/nl902647x · Cited by 239
Cell death during ischemia: relationship to mitochondrial depolarization and ROS generation
Levraut, J., Iwase, H., Shao, Z.-H., Vanden Hoek, T. L., & Schumacker, P. T. (2003) · American Journal of Physiology-Heart and Circulatory Physiology · doi:10.1152/ajpheart.00708.2002 · Cited by 225
Kim, J.-S., Yoon, T.-J., Yu, K.-N., Noh, M. S., Woo, M., Kim, B.-G., Lee, K.-H., Sohn, B.-H., Park, S.-B., Lee, J.-K., et al. (2006) · Journal of Veterinary Science · doi:10.4142/jvs.2006.7.4.321 · Cited by 212
Liu, Y. (2001) · Carcinogenesis · doi:10.1093/carcin/22.6.851 · Cited by 184
Wang, Y., Aker, W. G., Hwang, H.-M., Yedjou, C. G., Yu, H., & Tchounwou, P. B. (2011) · Science of The Total Environment · doi:10.1016/j.scitotenv.2011.07.039 · Cited by 172
Functional Assessment of Metal Oxide Nanoparticle Toxicity in Immune Cells
Maurer-Jones, M. A., Lin, Y.-S., & Haynes, C. L. (2010) · ACS Nano · doi:10.1021/nn9018834 · Cited by 171
C60 Binds to and Deforms Nucleotides
Zhao, X., Striolo, A., & Cummings, P. T. (2005) · Biophysical Journal · doi:10.1529/biophysj.105.064410 · Cited by 143
Nanosized Aluminum Altered Immune Function
Braydich‐Stolle, L. K., Speshock, J. L., Castle, A. B., Smith, M. J., Murdock, R. C., & Hussain, S. M. (2010) · ACS Nano · doi:10.1021/nn9016789 · Cited by 98
Kang, S. J., Kim, B. M., Lee, Y. J., Hong, S. H., & Chung, H. W. (2009) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2009.06.097 · Cited by 96
Cytotoxicity of Zinc Oxide Nanoparticles: Importance of Microenvironment
Yang, S., Liu, J.-H., Wang, J., Yuan, Y., Cao, A., Wang, H., Liu, Y., & Zhao, Y. (2010) · Journal of Nanoscience and Nanotechnology · doi:10.1166/jnn.2010.2491 · Cited by 77
Silica nanoconstruct cellular toleration threshold in vitro
Gustafson, H. H., Malugin, A., & Ghandehari, H. (2011) · Journal of Controlled Release · doi:10.1016/j.jconrel.2011.02.017 · Cited by 61
Zhang, C., Zhu, H., Yang, X., Lou, J.-S., Zhu, D., Lu, W. P., He, Q., & Yang, B. (2009) · Journal of Cancer Research and Clinical Oncology · doi:10.1007/s00432-009-0674-5 · Cited by 26
Cited by
Cellular uptake of nanoparticles: Journey inside the cell
Behzadi, S., Serpooshan, V., Tao, W., Hamaly, M. A., Alkawareek, M. Y., Dreaden, E. C., Brown, D., Alkilany, A. M., Farokhzad, O. C., & Mahmoudi, M. (2017) · Chemical Society Reviews · doi:10.1039/c6cs00636a · Cited by 2,859
The Toxic Effects and Mechanisms of CuO and ZnO Nanoparticles
Chang, Y.-N., Zhang, M., Xia, L., Zhang, J., & Xing, G. (2012) · Materials · doi:10.3390/ma5122850 · Cited by 772
Reactive Oxygen Species-Related Nanoparticle Toxicity in the Biomedical Field
Yu, Z., Li, Q., Wang, J., Yu, Y., Wang, Y., Zhou, Q., & Li, P. (2020) · Nanoscale Research Letters · doi:10.1186/s11671-020-03344-7 · Cited by 709
Antimicrobial Nano-Agents: The Copper Age
Ermini, M. L., & Voliani, V. (2021) · ACS Nano · doi:10.1021/acsnano.0c10756 · Cited by 477
Toxicity of copper oxide nanoparticles: a review study
Naz, S., Gul, A., & Zia, M. (2020) · IET Nanobiotechnology · doi:10.1049/iet-nbt.2019.0176 · Cited by 447
Metadata
- Authors
- Wang, Z., Li, N., Zhao, J., White, J. C., Qu, P., & Xing, B.
- Venue
- Chemical Research in Toxicology
- Publisher
- American Chemical Society (ACS)
- Published
- 2012-06-27
- Volume
- 25
- Issue
- 7
- Pages
- 1512-1521
- ISSN
- 0893-228X (ISSN-L)
- 1520-5010
- OpenAlex
- W2324506251
- Cited by
- 340
- References
- 43
- FWCI
- 11.00
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
