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Cited by 97Single-cell and spatial transcriptomicsMolecular Biology Techniques and ApplicationsNeuroscience and Neuropharmacology Research

The DOI matches its Crossref record; 107 of the 107 references it cites were found in a registry or an index.

Single-Cell Gene Expression Analysis: Implications for Neurodegenerative and Neuropsychiatric Disorders

Ginsberg, Stephen D., Elarova, Irina, Ruben, Marc, Tan, Fengzhu, Counts, Scott E., Eberwine, James H., Trojanowski, John Q., Hemby, Scott E., Mufson, Elliott J., Che, Shaoli·2004·Neurochemical Research
DOI 10.1023/b:nere.0000023593.77052.f7
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Abstract

Technical and experimental advances in microaspiration techniques, RNA amplification, quantitative real-time polymerase chain reaction (qPCR), and cDNA microarray analysis have led to an increase in the number of studies of single-cell gene expression. In particular, the central nervous system (CNS) is an ideal structure to apply single-cell gene expression paradigms. Unlike an organ that is composed of one principal cell type, the brain contains a constellation of neuronal and noneuronal populations of cells. A goal is to sample gene expression from similar cell types within a defined region without potential contamination by expression profiles of adjacent neuronal subpopulations and noneuronal cells. The unprecedented resolution afforded by single-cell RNA analysis in combination with cDNA microarrays and qPCR-based analyses allows for relative gene expression level comparisons across cell types under different experimental conditions and disease states. The ability to analyze single cells is an important distinction from global and regional assessments of mRNA expression and can be applied to optimally prepared tissues from animal models as well as postmortem human brain tissues. This focused review illustrates the potential power of single-cell gene expression studies within the CNS in relation to neurodegenerative and neuropsychiatric disorders such as Alzheimer's disease (AD) and schizophrenia, respectively.

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Crossref · 10.1023/b:nere.0000023593.77052.f7

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107 of 107 references verified0 citation errors · 0 not checkable

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  • Purifying cell populations or performing single-cell transcriptional analyses can identify important changes not apparent in whole-tissue transcriptomes.

    “Purifying cell populations or carrying out transcriptional analyses on single cells158–163 can identify important changes that are not apparent in whole-tissue transcriptomes141,142.”

    From Systems biology and gene networks in neurodevelopmental and neurodegenerative disorders (2015) · cites this paper as [158]

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Research works printed but not linked: 3

  1. Van Deerlin, V. M. D., Ginsberg, S. D., Lee, V. M-Y., and Trojanowski, J. Q. (2002). The use of fixed human post mortem brain tissue to study mRNA expression in neurodegenerative diseases: applications of microdissection and mRNA amplification. Pages 201–235, in Microarrays for the neurosciences: an essential guide, Geschwind, D. H. and Gregg, J. P, (eds.), MIT Press: Boston.
  2. Van Deerlin, V. M. D., Ginsberg, S. D., Lee, V. M-Y., and Trojanowski, J. Q. (2000). Fixed post mortem brain tissue for mRNA expression analysis in neurodegenerative diseases. Pages 118–128, in Geschwind, D. H. (ed.). DNA microarrays: the new frontier in gene discovery and gene expression analysis, Society for Neuroscience: Washington, DC.
  3. Ginsberg, S. D. 2001. Gene expression profiling using single cell microdissection combined with cDNA microarrays. Pages 61–70, in Geschwind, D. H. (ed.). DNA microarrays: the new frontier in gene discovery and gene expression analysis., Society for Neuroscience Press, Washington DC.

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Metadata

Authors
Ginsberg, S. D., Elarova, I., Ruben, M., Tan, F., Counts, S. E., Eberwine, J. H., Trojanowski, J. Q., Hemby, S. E., Mufson, E. J., & Che, S.
Affiliations
  • New York University (US)
  • Nathan Kline Institute for Psychiatric Research (US)
  • Rush University Medical Center (US)
  • University of Pennsylvania (US)
  • Emory University (US)
Corresponding author
Ginsberg, Stephen D.
ORCID
Venue
Neurochemical Research
Publisher
Springer Science and Business Media LLC
Published
2004-06
Volume
29
Issue
6
Pages
1053-1064
ISSN
  • 0364-3190 (ISSN-L)
  • 1573-6903
DOI
10.1023/b:nere.0000023593.77052.f7
OpenAlex
W2111247490
Cited by
97
References
107
Citations by year
2024: 1 · 2023: 2 · 2022: 1 · 2021: 2 · 2020: 1 · 2019: 2 · 2018: 2 · 2017: 1 · 2015: 5 · 2014: 4 · 2013: 5 · 2012: 2
FWCI
4.75
Type
journal article
Language
en
Open access
closed
Licence
Locations
  • Neurochemical Research (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health
  • National Institute on Aging (AG9900, AG 10161, AG-14449, AG09215, P01AG014449, AG021661, R01AG021661, P30AG010124, P01AG009215, AG09466, AG-10124, P30AG010161, AG 10668, R01AG043375, P01AG009466, R01AG010668)
  • National Institute of Mental Health (MH 43880)
  • National Cancer Institute (R21CA094520, CA94520)
  • National Institute of Neurological Disorders and Stroke (NS43939, R01NS043939)
  • NIH Clinical Center
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Single-cell and spatial transcriptomics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Molecular Biology Techniques and Applications
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
Keywords
single-cell gene expression, neurodegenerative diseases, neuropsychiatric disorders, Alzheimer's disease, schizophrenia, central nervous system, neuronal subpopulations, postmortem brain, animal models, gene expression profiling
MeSH terms
Cell Physiological Phenomena, Humans, Neurons, Receptors, Dopamine, Schizophrenia, Gene Expression, Prosencephalon, Neurodegenerative Diseases, Oligonucleotide Array Sequence Analysis, Gene Expression Profiling

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