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Cited by 488Sleep and Wakefulness ResearchCircadian rhythm and melatoninSleep and Work-Related Fatigue

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Stress response genes protect against lethal effects of sleep deprivation in Drosophila

Shaw, Paul J., Tononi, Giulio, Greenspan, Ralph J., Robinson, Donald F.·2002·Nature
DOI 10.1038/417287a
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Abstract

Sleep is controlled by two processes: a homeostatic drive that increases during waking and dissipates during sleep, and a circadian pacemaker that controls its timing. Although these two systems can operate independently, recent studies indicate a more intimate relationship. To study the interaction between homeostatic and circadian processes in Drosophila, we examined homeostasis in the canonical loss-of-function clock mutants period (per(01)), timeless (tim(01)), clock (Clk(jrk)) and cycle (cyc(01)). cyc(01) mutants showed a disproportionately large sleep rebound and died after 10 hours of sleep deprivation, although they were more resistant than other clock mutants to various stressors. Unlike other clock mutants, cyc(01) flies showed a reduced expression of heat-shock genes after sleep loss. However, activating heat-shock genes before sleep deprivation rescued cyc(01) flies from its lethal effects. Consistent with the protective effect of heat-shock genes, was the observation that flies carrying a mutation for the heat-shock protein Hsp83 (Hsp83(08445)) showed exaggerated homeostatic response and died after sleep deprivation. These data represent the first step in identifying the molecular mechanisms that constitute the sleep homeostat.

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  • Continuous sleep deprivation kills rodents and flies within a period of days to weeks.

    “continuous sleep deprivation kills rodents and flies within a period of days to weeks (5, 6).”

    From Sleep Drives Metabolite Clearance from the Adult Brain (2013) · cites this paper as [6]

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Metadata

Authors
Shaw, P. J., Tononi, G., Greenspan, R. J., & Robinson, D. F.
Affiliations
  • Neurosciences Institute (US)
  • University of Wisconsin–Madison (US)
ORCID
Venue
Nature
Publisher
Springer Science and Business Media LLC
Published
2002-05
Volume
417
Issue
6886
Pages
287-291
ISSN
  • 0028-0836 (ISSN-L)
  • 1476-4687
DOI
10.1038/417287a
OpenAlex
W1987605400
Cited by
488
References
0
Citations by year
2026: 19 · 2025: 27 · 2024: 19 · 2023: 17 · 2022: 19 · 2021: 20 · 2020: 25 · 2019: 26 · 2018: 18 · 2017: 20 · 2016: 23 · 2015: 28 · 2014: 19 · 2013: 25 · 2012: 13
FWCI
8.89
Type
journal article
Language
en
Open access
closed
Licence
www.springer.com/tdm (tdm)
Locations
  • Nature (publishedVersion)
  • PubMed (publishedVersion)
  • RePEc: Research Papers in Economics (submittedVersion)
Funders
  • Neurosciences Research Foundation
  • Neurosciences Foundation
  • National Institutes of Health
Topics
  • Life Sciences › Neuroscience › Cognitive Neuroscience › Sleep and Wakefulness Research
  • Life Sciences › Neuroscience › Endocrine and Autonomic Systems › Circadian rhythm and melatonin
  • Social Sciences › Psychology › Experimental and Cognitive Psychology › Sleep and Work-Related Fatigue
Keywords
sleep deprivation, sleep homeostasis, circadian clock, cycle gene, period, TIMELESS, clocks, heat shock genes, Hsp83, stress response genes, lethality, Drosophila
MeSH terms
Animals, Circadian Rhythm, Darkness, Dehydration, Drosophila melanogaster, Female, Food Deprivation, Gene Expression Regulation, Genes, Lethal, Heat-Shock Proteins, Homeostasis, Light, Mutation, Nuclear Proteins, Sleep, Sleep Deprivation, Stress, Physiological, Wakefulness, Trans-Activators, Survival Rate, Genes, Insect, Oxidative Stress, Heat-Shock Response, Insect Proteins, Drosophila Proteins, Basic Helix-Loop-Helix Proteins, CLOCK Proteins, ARNTL Transcription Factors, Period Circadian Proteins

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