Cited by 108Circadian rhythm and melatoninSleep and Work-Related FatigueDietary Effects on Health

Abnormal expressions of circadian-clock and circadian clock-controlled genes in the livers and kidneys of long-term, high-fat-diet-treated mice

Hsieh, M-C, Yang, S-C, Tseng, H-L, Hwang, L-L, Chen, C-T, Shieh, K-R·2010·International Journal of Obesity

Abstract

Objectives Physiological and behavioral circadian rhythmicities are exhibited by all mammals and are generated by intracellular levels of circadian oscillators, which are composed of transcriptional/translational feedback loops involving a set of circadian-clock genes, such as Clock, Per1-3, Cry1-2, Bmal1, Dbp, E4BP4 and CK1varepsilon. These circadian-clock genes play important roles in regulating circadian rhythms and also energy homeostasis and metabolism. Determining whether obesity induced by high-fat diet affected the expressions of circadian-clock genes and their related genes in peripheral tissues, was the main focus of this study. To address this issue, we fed male C57BL/6 mice a high-fat diet for 11 months to induce obesity, hyperglycemic, hypercholesterolemic and hyperinsulinemic symptoms, and used quantitative real-time reverse transcription-PCR to measure gene expression levels.Results We found that the expressions of circadian-clock genes and circadian clock-controlled genes, including Per1-3, Cry1-2, Bmal1, Dbp, E4BP4, CK1varepsilon, PEPCK, PDK4 and NHE3, were altered in the livers and/or kidneys.Conclusions These results indicate that obesity induced by high-fat diet alters the circadian-clock system, and obesity and metabolic syndrome are highly correlated with the expressions of circadian-clock genes and their downstream, circadian clock-controlled genes.

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Crossref · 10.1038/ijo.2009.228

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  • A high-fat diet may alter the function of the circadian clock, with an altered period of the locomotor activity rhythm and changes in the rhythms of core clock genes in adipose and liver tissue.

    “Even a high-fat diet may alter the function of the circadian clock, with an altered period of the locomotor activity rhythm and changes in the rhythms of the core clock genes in adipose and liver tissue [35–40,49].”

    From Circadian control of glucose metabolism (2014) · cites this paper as [37]

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Authors
Hsieh, M.-C., Yang, S.-C., Tseng, H.-L., Hwang, L.-L., Chen, C.-T., & Shieh, K.-R.
Affiliations
  • Tzu Chi College of Technology (TW)
  • Tzu Chi University (TW)
  • Taipei Medical University (TW)
  • National Health Research Institutes (TW)
Corresponding author
Shieh, K-R
Venue
International Journal of Obesity
Published
2009-11-10
Volume
34
Issue
2
Pages
227-239
ISSN
  • 0307-0565 (ISSN-L)
  • 1476-5497
OpenAlex
W1970711394
Cited by
108
References
60
Citations by year
2026: 2 · 2025: 3 · 2024: 4 · 2023: 6 · 2022: 5 · 2021: 7 · 2020: 10 · 2019: 3 · 2018: 10 · 2017: 8 · 2016: 5 · 2015: 5 · 2014: 7 · 2013: 10 · 2012: 6
FWCI
3.44
Type
journal article
Language
en
Open access
closed
Locations
  • International Journal of Obesity (publishedVersion)
  • PubMed (publishedVersion)
Funders
National Science Council
Topics
  • Life Sciences › Neuroscience › Endocrine and Autonomic Systems › Circadian rhythm and melatonin
  • Social Sciences › Psychology › Experimental and Cognitive Psychology › Sleep and Work-Related Fatigue
  • Health Sciences › Medicine › Physiology › Dietary Effects on Health
Keywords
clock genes, high-fat diet, obesity, metabolic syndrome, liver, kidney, Bmal1, DBP, E4BP4, CK1ε, phosphoenolpyruvate carboxykinase, PDK4, NHE3, gene expression, energy homeostasis
MeSH terms
Animals, Body Weight, Circadian Rhythm, Dietary Fats, Gene Expression Regulation, Kidney, Liver, Male, Mice, Inbred C57BL, Obesity, Trans-Activators, Metabolic Syndrome, Mice, Circadian Rhythm Signaling Peptides and Proteins, CLOCK Proteins

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