Brain and muscle Arnt-like protein-1 (BMAL1), a component of the molecular clock, regulates adipogenesis

Shimba, Shigeki; Ishii, Norimasa; Ohta, Yuki; Ohno, Toshiharu; Watabe, Yuichi; Hayashi, Mitsuaki; Wada, Taira; Aoyagi, Toshinori; Tezuka, Masakatsu

doi:10.1073/pnas.0502383102

Cited by 536Circadian rhythm and melatoninAdipose Tissue and MetabolismPeroxisome Proliferator-Activated Receptors

Brain and muscle Arnt-like protein-1 (BMAL1), a component of the molecular clock, regulates adipogenesis

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Shimba, Shigeki, Ishii, Norimasa, Ohta, Yuki, Ohno, Toshiharu, Watabe, Yuichi, Hayashi, Mitsuaki, Wada, Taira, Aoyagi, Toshinori +1 more·2005·Proceedings of the National Academy of Sciences

DOI 10.1073/pnas.0502383102PMC PMC1189312

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Abstract

Brain and muscle Arnt-like protein-1 (BMAL1; also known as MOP3 or Arnt3) is a transcription factor known to regulate circadian rhythm. Here, we established its involvement in the control of adipogenesis and lipid metabolism activity in mature adipocytes. During adipose differentiation in 3T3-L1 cells, the level of BMAL1 mRNA began to increase 4 days after induction and was highly expressed in differentiated cells. In white adipose tissues isolated from C57BL/6J mice, BMAL1 was predominantly expressed in a fraction containing adipocytes, as compared with the stromal-vascular fraction. BMAL1 knockout mice embryonic fibroblast cells failed to be differentiated into adipocytes. Importantly, adding BMAL1 back by adenovirus gene transfer restored the ability of BMAL1 knockout mice embryonic fibroblast cells to differentiate. Knock-down of BMAL1 expression in 3T3-L1 cells by an RNA interference technique allowed the cells to accumulate only minimum amounts of lipid droplets in the cells. Adenovirus-mediated expression of BMAL1 in 3T3-L1 adipocytes resulted in induction of several factors involved in lipogenesis. The promoter activity of these genes was stimulated in a BMAL1-dependent manner. Interestingly, expression of these factors showed clear circadian rhythm in mice adipose tissue. Furthermore, overexpression of BMAL1 in adipocytes increased lipid synthesis activity. These results indicate that BMAL1, a master regulator of circadian rhythm, also plays important roles in the regulation of adipose differentiation and lipogenesis in mature adipocytes.

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Knockout of Bmal1 alone in C57BL/6J mice showed that isolated mouse embryonic fibroblast (MEF) cells could not successfully differentiate into adipocytes.
“Knockout of Bmal1 alone in C57BL/6J mice showed that isolated mouse embryonic fibroblast (MEF) cells could not successfully differentiate into adipocytes (Shimba et al., 2005).”
From The impact of the circadian timing system on cardiovascular and metabolic function (2012) · cites this paper as Shimba et al., 2005
The presence of high Bmal1 mRNA levels in adipocytes increased lipogenesis activity, further suggesting that Bmal1 is involved in regulating lipid metabolism.
“The presence of high Bmal1 mRNA levels in adipocytes increased lipogenesis activity, further suggesting that Bmal1 is involved in regulating lipid metabolism.”
From The impact of the circadian timing system on cardiovascular and metabolic function (2012) · cites this paper as Shimba et al., 2005
Restoration of Bmal1 function by adenovirus gene transfer into the initial Bmal1-deficient mice restored adipocyte differentiation and lipid accumulation in the MEF cells.
“Further, restoration of Bmal1 function by adenovirus gene transfer into the initial Bmal1-deficient mice restored adipocyte differentiation and lipid accumulation in the MEF cells.”
From The impact of the circadian timing system on cardiovascular and metabolic function (2012) · cites this paper as Shimba et al., 2005

A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
Bradford, M. M. (1976) · Analytical Biochemistry · doi:10.1016/0003-2697(76)90527-3 · Cited by 255,302 · see citing works
Coordination of circadian timing in mammals
Reppert, S. M., & Weaver, D. R. (2002) · Nature · doi:10.1038/nature00965 · Cited by 4,379 · see citing works
Molecular Bases for Circadian Clocks
Dunlap, J. C. (1999) · Cell · doi:10.1016/s0092-8674(00)80566-8 · Cited by 2,900 · see citing works
Obesity and Metabolic Syndrome in Circadian Clock Mutant Mice
Turek, F. W., Joshu, C., Kohsaka, A., Lin, E., Ivanova, G., McDearmon, E., Laposky, A., Losee-Olson, S., Easton, A., Jensen, D. R., et al. (2005) · Science · doi:10.1126/science.1108750 · Cited by 2,485 · see citing works
Obesity and the regulation of energy balance
Spiegelman, B. M., & Flier, J. S. (2001) · Cell · doi:10.1016/s0092-8674(01)00240-9 · Cited by 2,398 · see citing works
Mop3 is an essential component of the master circadian pacemaker in mammals.
Bunger, M. K., Wilsbacher, L. D., Moran, S. M., Clendenin, C., Radcliffe, L. A., Hogenesch, J. B., Simon, M. C., Takahashi, J. S., & Bradfield, C. A. (2000) · Cell · doi:10.1016/s0092-8674(00)00205-1 · Cited by 1,621 · see citing works
Adipose Tissue as an Endocrine Organ
Ahima, R. S., & Flier, J. S. (2000) · Trends in Endocrinology & Metabolism · doi:10.1016/s1043-2760(00)00301-5 · Cited by 1,608 · see citing works
Time zones: a comparative genetics of circadian clocks
Young, M. W., & Kay, S. A. (2001) · Nature Reviews Genetics · doi:10.1038/35088576 · Cited by 1,143 · see citing works
BMAL1 and CLOCK, two essential components of the circadian clock, are involved in glucose homeostasis
Rudic, R. D., McNamara, P., Curtis, A.-M., Boston, R. C., Panda, S., Hogenesch, J. B., & FitzGerald, G. A. (2004) · PLoS Biology · doi:10.1371/journal.pbio.0020377 · Cited by 1,059 · see citing works
Regulation of clock and NPAS2 DNA binding by the redox state of NAD cofactors
Rutter, J., Reick, M., Wu, L. C., & McKnight, S. L. (2001) · Science · doi:10.1126/science.1060698 · Cited by 1,059 · see citing works
ADD1/SREBP1 promotes adipocyte differentiation and gene expression linked to fatty acid metabolism.Preprint
Kim, J. B., & Spiegelman, B. M. (1996) · Genes & Development · doi:10.1101/gad.10.9.1096 · Cited by 1,026 · see citing works
Mammalian circadian biology: elucidating genome-wide levels of temporal organization
Lowrey, P. L., & Takahashi, J. S. (2004) · Annual Review of Genomics and Human Genetics · doi:10.1146/annurev.genom.5.061903.175925 · Cited by 976 · see citing works
Overproduction of cholesterol and fatty acids causes massive liver enlargement in transgenic mice expressing truncated SREBP-1a.
Shimano, H., Horton, J. D., Hammer, R. E., Shimomura, I., Brown, M. S., & Goldstein, J. L. (1996) · Journal of Clinical Investigation · doi:10.1172/jci118951 · Cited by 810 · see citing works
The basic-helix–loop–helix-PAS orphan MOP3 forms transcriptionally active complexes with circadian and hypoxia factors
Hogenesch, J. B., Gu, Y.-Z., Jain, S. R., & Bradfield, C. A. (1998) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.95.10.5474 · Cited by 781 · see citing works
Differential expression of exons 1a and 1c in mRNAs for sterol regulatory element binding protein-1 in human and mouse organs and cultured cells.
Shimomura, I., Shimano, H., Horton, J. D., Goldstein, J. L., & Brown, M. S. (1997) · Journal of Clinical Investigation · doi:10.1172/jci119247 · Cited by 760 · see citing works
ADD1/SREBP1 activates PPARγ through the production of endogenous ligand
Kim, J. B., Wright, H. M., Wright, M., & Spiegelman, B. M. (1998) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.95.8.4333 · Cited by 670 · see citing works
Two Tandem Binding Sites for Sterol Regulatory Element Binding Proteins Are Required for Sterol Regulation of Fatty-acid Synthase Promoter
Magaña, M., & Osborne, T. F. (1996) · Journal of Biological Chemistry · doi:10.1074/jbc.271.51.32689 · Cited by 336 · see citing works
The Orphan Nuclear Receptor Rev-Erbα Is a Peroxisome Proliferator-activated Receptor (PPAR) γ Target Gene and Promotes PPARγ-induced Adipocyte Differentiation
Fontaine, C., Dubois, G., Duguay, Y., Helledie, T., Vu‐Dac, N., Gervois, P. P., Soncin, F., Mandrup, S., Fruchart, J., Fruchart‐Najib, J., et al. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m304664200 · Cited by 248 · see citing works
BMAL1-dependent circadian oscillation of nuclear CLOCK: posttranslational events induced by dimerization of transcriptional activators of the mammalian clock systemPreprint
Kondratov, R. V., Chernov, M. V., Kondratova, A. A., Gorbacheva, V., Gudkov, A. V., & Antoch, M. P. (2003) · Genes & Development · doi:10.1101/gad.1099503 · Cited by 238 · see citing works
Overexpression of Sterol Regulatory Element-binding Protein-1a in Mouse Adipose Tissue Produces Adipocyte Hypertrophy, Increased Fatty Acid Secretion, and Fatty Liver
Horton, J. D., Shimomura, I., Ikemoto, S., Bashmakov, Y. K., & Hammer, R. E. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m306540200 · Cited by 229 · see citing works
Identification of Glycerol-3-phosphate Acyltransferase as an Adipocyte Determination and Differentiation Factor 1- and Sterol Regulatory Element-binding Protein-responsive Gene
Ericsson, J., Jackson, S. M., Kim, J. B., Spiegelman, B. M., & Edwards, P. A. (1997) · Journal of Biological Chemistry · doi:10.1074/jbc.272.11.7298 · Cited by 227 · see citing works
cDNA Cloning and Tissue-Specific Expression of a Novel Basic Helix–Loop–Helix/PAS Protein (BMAL1) and Identification of Alternatively Spliced Variants with Alternative Translation Initiation Site Usage
Ikeda, M., & Nomura, M. (1997) · Biochemical and Biophysical Research Communications · doi:10.1006/bbrc.1997.6371 · Cited by 220 · see citing works
Progressive arthropathy in mice with a targeted disruption of theMop3/Bmal-1 locus
Bunger, M. K., Walisser, J. A., Sullivan, R., Manley, P. A., Moran, S. M., Kalscheur, V. L., Colman, R. J., & Bradfield, C. A. (2005) · genesis · doi:10.1002/gene.20102 · Cited by 207 · see citing works
Induction of Rev-ErbA alpha, an orphan receptor encoded on the opposite strand of the alpha-thyroid hormone receptor gene, during adipocyte differentiation
Chawla, A., & Lazar, M. A. (1993) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)85415-7 · Cited by 198 · see citing works
Circadian variations of blood sugar and plasma insulin levels in man
Malherbe, C., Marc De Gasparo, B., Hertogh, R. D., & Hoem, J. J. (1969) · Diabetologia · doi:10.1007/bf00427978 · Cited by 167 · see citing works
The orphan receptor Rev-erb α gene is a target of the circadian clock pacemaker
Triqueneaux, G., Thénot, S., Kakizawa, T., Antoch, M. P., Safi, R., Takahashi, J. S., Delaunay, F., & Laudet, V. (2004) · Journal of Molecular Endocrinology · doi:10.1677/jme.1.01554 · Cited by 166 · see citing works
Transcriptionally Active Heterodimer Formation of an Arnt-like PAS Protein, Arnt3, with HIF-1a, HLF, and Clock
Takahata, S., Sogawa, K., Kobayashi, A., Ema, M., Mimura, J., Ozaki, N., & Fujii‐Kuriyama, Y. (1998) · Biochemical and Biophysical Research Communications · doi:10.1006/bbrc.1998.9012 · Cited by 153 · see citing works
Diurnal Patterns of Triglycerides, Free Fatty Acids, Blood Sugar, and Insulin during Carbohydrate-Induction in Man and Their Modification by Nocturnal Suppression of Lipolysis
Schlierf, G., & Dorow, E. (1973) · Journal of Clinical Investigation · doi:10.1172/jci107235 · Cited by 148 · see citing works
Transcription Regulation within the Circadian Clock: The E-box and Beyond
Hardin, P. E. (2004) · Journal of Biological Rhythms · doi:10.1177/0748730404268052 · Cited by 148 · see citing works
SREBP-1 Interacts with Hepatocyte Nuclear Factor-4α and Interferes with PGC-1 Recruitment to Suppress Hepatic Gluconeogenic Genes
Yamamoto, T., Shimano, H., Nakagawa, Y., Ide, T., Yahagi, N., Matsuzaka, T., Nakakuki, M., Takahashi, A., Suzuki, H., Sone, H., et al. (2004) · Journal of Biological Chemistry · doi:10.1074/jbc.m310333200 · Cited by 143 · see citing works
Adenovirus-Mediated Overexpression of Sterol Regulatory Element Binding Protein-1c Mimics Insulin Effects on Hepatic Gene Expression and Glucose Homeostasis in Diabetic Mice
Bécard, D., Hainault, I., Azzout‐Marniche, D., Bertry-Coussot, L., Ferré, P., & Foufelle, F. (2001) · Diabetes · doi:10.2337/diabetes.50.11.2425 · Cited by 133 · see citing works
Sterol regulation of acetyl coenzyme A carboxylase promoter requires two interdependent binding sites for sterol regulatory element binding proteins
Magana, M. M., Lin, S. S., Dooley, K. A., & Osborne, T. F. (1997) · Journal of Lipid Research · doi:10.1016/s0022-2275(20)37181-9 · Cited by 124 · see citing works
Sterol Regulatory Element-binding Protein-1c Mimics the Negative Effect of Insulin on Phosphoenolpyruvate Carboxykinase (GTP) Gene Transcription
Chakravarty, K., Leahy, P. S., Bécard, D., Hakimi, P., Foretz, M., Ferré, P., Foufelle, F., & Hanson, R. W. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.m103310200 · Cited by 92 · see citing works
Circadian Periodicity in Some Physicochemical Parameters of Circulating Blood
Seaman, G. V. F., ENGEL, R., SWANK, R. L., & HISSEN, W. (1965) · Nature · doi:10.1038/207833a0 · Cited by 90 · see citing works
SREBP-1c and Sp1 Interact to Regulate Transcription of the Gene for Phosphoenolpyruvate Carboxykinase (GTP) in the Liver
Chakravarty, K., Wu, S.-Y., Chiang, C.-M., Samols, D. R., & Hanson, R. W. (2004) · Journal of Biological Chemistry · doi:10.1074/jbc.m309905200 · Cited by 69 · see citing works
Alteration of the 4-sphingenine scaffolds of ceramides in keratinocyte-specific Arnt-deficient mice affects skin barrier function
Takagi, S., Tojo, H., Tomita, S., Sano, S., Itami, S., Hara, M., Inoue, S., Horie, K., Kondoh, G., Hosokawa, K., et al. (2003) · Journal of Clinical Investigation · doi:10.1172/jci200318513 · Cited by 59 · see citing works
Regulation of adiponectin receptor R1 and R2 gene expression in adipocytes of C57BL/6 mice
Blüher, M., Faßhauer, M., Kralisch, S., Schön, M. R., Krohn, K., & Paschke, R. (2005) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2005.02.081 · Cited by 53 · see citing works
Circadian Variation of the Serum Glucose and Immunoreactive Insulin Levels
Gagliardino, J. J., & HERNÉNDEZ, R. E. (1971) · Endocrinology · doi:10.1210/endo-88-6-1529 · Cited by 49 · see citing works
Characteristics of Circadian Gene Expressions in Mice White Adipose Tissue and 3T3-L1 Adipocytes
Aoyagi, T., Shimba, S., & Tezuka, M. (2005) · Journal of Health Science · doi:10.1248/jhs.51.21 · Cited by 37 · see citing works
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Adipocyte differentiation from the inside out
Rosen, E. D., & MacDougald, O. A. (2006) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2066 · Cited by 2,607 · see citing works
Transcriptional control of adipocyte formation
Farmer, S. R. (2006) · Cell Metabolism · doi:10.1016/j.cmet.2006.07.001 · Cited by 1,836 · see citing works
Molecular components of the mammalian circadian clock
Ko, C. H., & Takahashi, J. S. (2006) · Human Molecular Genetics · doi:10.1093/hmg/ddl207 · Cited by 1,657 · see citing works
The genetics of mammalian circadian order and disorder: implications for physiology and disease
Takahashi, J. S., Hong, H.-K., Ko, C. H., & McDearmon, E. L. (2008) · Nature Reviews Genetics · doi:10.1038/nrg2430 · Cited by 1,611 · see citing works
High-Fat Diet Disrupts Behavioral and Molecular Circadian Rhythms in Mice
Kohsaka, A., Laposky, A. D., Ramsey, K. M., Estrada, C., Joshu, C., Kobayashi, Y., Turek, F. W., & Bass, J. (2007) · Cell Metabolism · doi:10.1016/j.cmet.2007.09.006 · Cited by 1,528 · see citing works
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Ghaben, A. L., & Scherer, P. E. (2019) · Nature Reviews Molecular Cell Biology · doi:10.1038/s41580-018-0093-z · Cited by 1,395 · see citing works
Early aging and age-related pathologies in mice deficient in BMAL1, the core componentof the circadian clock
Kondratov, R. V., Kondratova, A. A., Gorbacheva, V. Y., Vykhovanets, O. V., & Antoch, M. P. (2006) · Genes & Development · doi:10.1101/gad.1432206 · Cited by 1,188 · see citing works
Physiological significance of a peripheral tissue circadian clock
Lamia, K. A., Storch, K.-F., & Weitz, C. J. (2008) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0806717105 · Cited by 1,048 · see citing works
The Meter of Metabolism
Green, C. B., Takahashi, J. S., & Bass, J. (2008) · Cell · doi:10.1016/j.cell.2008.08.022 · Cited by 902 · see citing works
New developments in adipogenesis
Lefterova, M. I., & Lazar, M. A. (2009) · Trends in Endocrinology and Metabolism · doi:10.1016/j.tem.2008.11.005 · Cited by 861 · see citing works
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Huang, W., Ramsey, K. M., Marcheva, B., & Bass, J. T. (2011) · Journal of Clinical Investigation · doi:10.1172/jci46043 · Cited by 726 · see citing works
Identification of heme as the ligand for the orphan nuclear receptors REV-ERBα and REV-ERBβ
Raghuram, S., Stayrook, K. R., Huang, P., Rogers, P. M., Nosie, A. K., McClure, D. B., Burris, L. L., Khorasanizadeh, S., Burris, T. P., & Rastinejad, F. (2007) · Nature Structural & Molecular Biology · doi:10.1038/nsmb1344 · Cited by 560 · see citing works
Genomics of circadian rhythms in health and disease
Rijo‐Ferreira, F., & Takahashi, J. S. (2019) · Genome Medicine · doi:10.1186/s13073-019-0704-0 · Cited by 537 · see citing works
Metabolism and the Circadian Clock Converge
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Froy, O. (2010) · Endocrine Reviews · doi:10.1210/er.2009-0014 · Cited by 505 · see citing works
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Aryl hydrocarbon receptor nuclear translocator-like (BMAL1) is associated with susceptibility to hypertension and type 2 diabetes
Woon, P. Y., Kaisaki, P. J., Bragança, J., Bihoreau, M., Lévy, J., Farrall, M., & Gauguier, D. (2007) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0703247104 · Cited by 410 · see citing works
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Kang, S., Bennett, C. N., Gerin, I., Rapp, L. A., Hankenson, K. D., & MacDougald, O. A. (2007) · Journal of Biological Chemistry · doi:10.1074/jbc.m700030200 · Cited by 400 · see citing works
The Impact of Sleep and Circadian Disturbance on Hormones and Metabolism
Kim, T., Jeong, J., & Hong, S. (2015) · International Journal of Endocrinology · doi:10.1155/2015/591729 · Cited by 378 · see citing works
Reciprocal Regulation of Brain and Muscle Arnt-Like Protein 1 and Peroxisome Proliferator-Activated Receptor α Defines a Novel Positive Feedback Loop in the Rodent Liver Circadian Clock
Canaple, L., Rambaud, J., Dkhissi‐Benyahya, O., Rayet, B., Tan, N. S., Michalik, L., Delaunay, F., Wahli, W., & Laudet, V. (2006) · Molecular Endocrinology · doi:10.1210/me.2006-0052 · Cited by 353 · see citing works
Sleep and circadian rhythms: key components in the regulation of energy metabolism
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Self-citation
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BOARD-INVITED REVIEW: The biology and regulation of preadipocytes and adipocytes in meat animals1,2
Hausman, G., Dodson, M. V., Ajuwon, K. M., Azain, M. J., Barnes, K. M., Guan, L. L., Jiang, Z., Poulos, S. P., Sainz, R. D., Smith, S. B., et al. (2008) · Journal of Animal Science · doi:10.2527/jas.2008-1427 · Cited by 343 · see citing works
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Sahar, S., Zocchi, L., Kinoshita, C., Borrelli, E., & Sassone‐Corsi, P. (2009) · PLoS ONE · doi:10.1371/journal.pone.0008561 · Cited by 291 · see citing works
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Jagannath, A., Taylor, L., Wakaf, Z., Vasudevan, S. R., & Foster, F. (2017) · Human Molecular Genetics · doi:10.1093/hmg/ddx240 · Cited by 258 · see citing works
Hypoxia, hypoxia-inducible factors (HIF), HIF hydroxylases and oxygen sensing
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Impact of clock-associated Arabidopsis pseudo-response regulators in metabolic coordination
Fukushima, A., Kusano, M., Nakamichi, N., Kobayashi, M., Hayashi, N., Sakakibara, H., Mizuno, T., & Saito, K. (2009) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0900952106 · Cited by 249 · see citing works
Circadian regulation of lipid mobilization in white adipose tissues
Shostak, A., Meyer-Kovac, J., & Oster, H. (2013) · Diabetes · doi:10.2337/db12-1449 · Cited by 243 · see citing works
Circadian rhythms in glucose and lipid metabolism in nocturnal and diurnal mammals
Kumar Jha, P., Challet, E., & Kalsbeek, A. (2015) · Molecular and Cellular Endocrinology · doi:10.1016/j.mce.2015.01.024 · Cited by 235 · see citing works
A sense of time: how molecular clocks organize metabolism
Kohsaka, A., & Bass, J. T. (2006) · Trends in Endocrinology and Metabolism · doi:10.1016/j.tem.2006.11.005 · Cited by 233 · see citing works
Regulation of metabolism: the circadian clock dictates the time
Sahar, S., & Sassone-Corsi, P. (2012) · Trends in Endocrinology & Metabolism · doi:10.1016/j.tem.2011.10.005 · Cited by 222 · see citing works
Impact of nutrients on circadian rhythmicity
Oosterman, J. E., Kalsbeek, A., la Fleur, S. E., & Belsham, D. D. (2015) · American Journal of Physiology-Regulatory, Integrative and Comparative Physiology · doi:10.1152/ajpregu.00322.2014 · Cited by 221 · see citing works
Loss of Nocturnin, a circadian deadenylase, confers resistance to hepatic steatosis and diet-induced obesity
Green, C. B., Douris, N., Kojima, S., Strayer, C. A., Fogerty, J., Lourim, D., Keller, S. R., & Besharse, J. C. (2007) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0702448104 · Cited by 215 · see citing works
The impact of the circadian timing system on cardiovascular and metabolic function
Morris, C. J., Yang, J. N., & Scheer, F. A. J. L. (2012) · Progress in Brain Research · doi:10.1016/b978-0-444-59427-3.00019-8 · Cited by 215 · see citing works
Characterization of the Molecular Clock in Mouse Peritoneal Macrophages
Hayashi, M., Shimba, S., & Tezuka, M. (2007) · Biological and Pharmaceutical Bulletin · doi:10.1248/bpb.30.621 · Cited by 206 · see citing works
Self-citation
THE CROSSTALK BETWEEN PHYSIOLOGY AND CIRCADIAN CLOCK PROTEINS
Duguay, D., & Cermakian, N. (2009) · Chronobiology International · doi:10.3109/07420520903497575 · Cited by 201 · see citing works
Circadian Etiology of Type 2 Diabetes Mellitus
Javeed, N., & Matveyenko, A. V. (2018) · Physiology · doi:10.1152/physiol.00003.2018 · Cited by 205 · see citing works
Association of night eating habits with metabolic syndrome and its components: a longitudinal study
Yoshida, J., Eguchi, E., Nagaoka, K., Ito, T., & Ogino, K. (2018) · BMC Public Health · doi:10.1186/s12889-018-6262-3 · Cited by 198 · see citing works
Circadian disruption and metabolic disease: Findings from animal models
Arble, D. M., Ramsey, K. M., Bass, J., & Turek, F. W. (2010) · Best Practice & Research Clinical Endocrinology & Metabolism · doi:10.1016/j.beem.2010.08.003 · Cited by 192 · see citing works
Cryptochrome, Circadian Cycle, Cell Cycle Checkpoints, and Cancer
Gauger, M. A., & Sancar, A. (2005) · Cancer Research · doi:10.1158/0008-5472.can-05-1119 · Cited by 181 · see citing works
Chronobiological aspects of nutrition, metabolic syndrome and obesity
Garaulet, M., & Madrid, J. A. (2010) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2010.05.005 · Cited by 177 · see citing works
The endogenous molecular clock orchestrates the temporal separation of substrate metabolism in skeletal muscle
Hodge, B. A., Wen, Y., Riley, L. A., Zhang, X., England, J. H., Harfmann, B. D., Schroder, E. A., & Esser, K. A. (2015) · Skeletal Muscle · doi:10.1186/s13395-015-0039-5 · Cited by 175 · see citing works
Rev-erb-α: an integrator of circadian rhythms and metabolism
Duez, H., & Staels, B. (2009) · Journal of Applied Physiology · doi:10.1152/japplphysiol.00570.2009 · Cited by 175 · see citing works
High-fat diet-induced hyperinsulinemia and tissue-specific insulin resistance in Cry -deficient mice
Barclay, J. L., Shostak, A., Leliavski, A., Tsang, A. H., Jöhren, O., Müller-Fielitz, H., Landgraf, D., Naujokat, N., van der Horst, G. T. J., & Oster, H. (2013) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00512.2012 · Cited by 173 · see citing works
Identification and characterization of genes that control fat deposition in chickens
D’Andre, H. C., Paul, W., Shen, X., Jia, X., Zhang, R., Sun, L., & Zhang, X. (2013) · Journal of Animal Science and Biotechnology/Journal of animal science and biotechnology · doi:10.1186/2049-1891-4-43 · Cited by 168 · see citing works
The clock gene, brain and muscle Arnt‐like 1, regulates adipogenesis via Wnt signaling pathway
Guo, B., Chatterjee, S., Li, L., Kim, J. M., Lee, J., Yechoor, V. K., Minze, L. J., Hsueh, W. A., & Ma, K. (2012) · The FASEB Journal · doi:10.1096/fj.12-205781 · Cited by 168 · see citing works
Prevention of Diabetes and Cardiovascular Disease in Obesity
Sala, L. L., & Pontiroli, A. E. (2020) · International Journal of Molecular Sciences · doi:10.3390/ijms21218178 · Cited by 171 · see citing works
When the Clock stops ticking, metabolic syndrome explodes
Staels, B. (2006) · Nature Medicine · doi:10.1038/nm0106-54 · Cited by 167 · see citing works
Disrupted fat absorption attenuates obesity induced by a high‐fat diet in Clock mutant mice
Oishi, K., Atsumi, G.-I., Sugiyama, S., Kodomari, I., Kasamatsu, M., Machida, K., & Ishida, N. (2006) · FEBS Letters · doi:10.1016/j.febslet.2005.11.063 · Cited by 166 · see citing works
Clock Genes and Clock-Controlled Genes in the Regulation of Metabolic Rhythms
Mazzoccoli, G., Pazienza, V., & Vinciguerra, M. (2012) · Chronobiology International · doi:10.3109/07420528.2012.658127 · Cited by 171 · see citing works
Adipose Organ Development and Remodeling
Cinti, S. (2018) · Comprehensive physiology · doi:10.1002/cphy.c170042 · Cited by 187 · see citing works
Free radicals and male reproduction.
Agarwal, A. K., & Allamaneni, S. S. R. (2011) · PubMed · Cited by 154 · see citing works
Circadian rhythms in gene expression: Relationship to physiology, disease, drug disposition and drug action☆
Sukumaran, S., Almon, R. R., DuBois, D. C., & Jusko, W. J. (2010) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2010.05.009 · Cited by 160 · see citing works
Clock genes are implicated in the human metabolic syndrome
Gómez-Abellán, P., Morante, J. J. H., Luján, J., Madrid, J. A., & Garaulet, M. (2007) · International Journal of Obesity · doi:10.1038/sj.ijo.0803689 · Cited by 160 · see citing works
Circadian clocks in the digestive system
Segers, A., & Depoortere, I. (2021) · Nature Reviews Gastroenterology & Hepatology · doi:10.1038/s41575-020-00401-5 · Cited by 160 · see citing works
Torpor induction in mammals: recent discoveries fueling new ideas
Melvin, R. G., & Andrews, M. T. (2009) · Trends in Endocrinology and Metabolism · doi:10.1016/j.tem.2009.09.005 · Cited by 159 · see citing works
Nuclear Hormone Receptors for Heme: REV-ERBα and REV-ERBβ Are Ligand-Regulated Components of the Mammalian Clock
Burris, T. P. (2008) · Molecular Endocrinology · doi:10.1210/me.2007-0519 · Cited by 155 · see citing works
A role of the circadian system and circadian proteins in aging
KONDRATOV, R. (2007) · Ageing Research Reviews · doi:10.1016/j.arr.2007.02.003 · Cited by 149 · see citing works
Circadian Rhythms, Aging, and Life Span in Mammals
Froy, O. (2011) · Physiology · doi:10.1152/physiol.00012.2011 · Cited by 156 · see citing works
Chronobiology, genetics and metabolic syndrome
Garaulet, M., & Madrid, J. A. (2009) · Current Opinion in Lipidology · doi:10.1097/mol.0b013e3283292399 · Cited by 157 · see citing works
The role of the circadian clock system in physiology
Pilorz, V., Helfrich‐Förster, C., & Oster, H. (2018) · Pflügers Archiv - European Journal of Physiology · doi:10.1007/s00424-017-2103-y · Cited by 156 · see citing works
Time-of-day-dependent dietary fat consumption influences multiple cardiometabolic syndrome parameters in mice
Bray, M. S., Tsai, J.-Y., Villegas‐Montoya, C., Boland, B. B., Blasier, Z., Egbejimi, O., Kueht, M., & Young, M. E. (2010) · International Journal of Obesity · doi:10.1038/ijo.2010.63 · Cited by 151 · see citing works
Prolonged daily light exposure increases body fat mass through attenuation of brown adipose tissue activity
Kooijman, S., van den Berg, R., Ramkisoensing, A., Boon, M. R., Kuipers, E. N., Loef, M., Zonneveld, T. C. M., Lucassen, E. A., Sips, H. C. M., Chatzispyrou, I. A., et al. (2015) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1504239112 · Cited by 150 · see citing works
Overexpression of the Circadian Clock Gene Bmal1 Increases Sensitivity to Oxaliplatin in Colorectal Cancer
Zeng, Z.-L., Luo, H., Yang, J., Wu, W.-J., Chen, D.-L., Huang, P., & Xu, R. (2013) · Clinical Cancer Research · doi:10.1158/1078-0432.ccr-13-0171 · Cited by 150 · see citing works
A circadian-regulated gene, Nocturnin , promotes adipogenesis by stimulating PPAR-γ nuclear translocation
Kawai, M., Green, C. B., Lecka‐Czernik, B., Douris, N., Gilbert, M. R., Kojima, S., Ackert‐Bicknell, C. L., Garg, N., Horowitz, M. C., Adamo, M. L., et al. (2010) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1000788107 · Cited by 146 · see citing works
Role of melatonin in metabolic regulation
Korkmaz, A., Topal, T., Tan, D., & Reiter, R. J. (2009) · Reviews in Endocrine and Metabolic Disorders · doi:10.1007/s11154-009-9117-5 · Cited by 145 · see citing works
Chronotherapeutic strategy: Rhythm monitoring, manipulation and disruption☆
Ohdo, S. (2010) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2010.01.006 · Cited by 146 · see citing works
Circadian rhythm in pharmacokinetics and its relevance to chronotherapy
Dong, D., Yang, D., Lin, L., Wang, S., & Wu, B. (2020) · Biochemical Pharmacology · doi:10.1016/j.bcp.2020.114045 · Cited by 146 · see citing works
O-GlcNAcylation, Novel Post-Translational Modification Linking Myocardial Metabolism and Cardiomyocyte Circadian Clock
Durgan, D. J., Pat, B., Laczy, B., Bradley, J. A., Tsai, J.-Y., Grenett, M. H., Ratcliffe, W. F., Brewer, R. A., Nagendran, J., Villegas‐Montoya, C., et al. (2011) · Journal of Biological Chemistry · doi:10.1074/jbc.m111.278903 · Cited by 144 · see citing works
Circadian Rhythms in Diet-Induced Obesity
Engin, A. (2017) · Advances in experimental medicine and biology · doi:10.1007/978-3-319-48382-5_2 · Cited by 144 · see citing works
Metabolic homeostasis in mice with disruptedClockgene expression in peripheral tissues
Kennaway, D. J., Owens, J. A., Voultsios, A., Boden, M. J., & Varcoe, T. J. (2007) · American Journal of Physiology-Regulatory, Integrative and Comparative Physiology · doi:10.1152/ajpregu.00018.2007 · Cited by 140 · see citing works
Effect of feeding regimens on circadian rhythms: Implications for agingand longevity
Froy, O., & Miskin, R. (2010) · Aging · doi:10.18632/aging.100116 · Cited by 141 · see citing works
High-fat diet delays and fasting advances the circadian expression of adiponectin signaling components in mouse liver
Barnea, M., Madar, Z., & Froy, O. (2009) · Endocrinology · doi:10.1210/en.2008-0944 · Cited by 138 · see citing works
Disruption of Bmal1 Impairs Blood–Brain Barrier Integrity via Pericyte Dysfunction
Nakazato, R., Kawabe, K., Yamada, D., Ikeno, S., Mieda, M., Shimba, S., Hinoi, E., Yoneda, Y., & Takarada, T. (2017) · Journal of Neuroscience · doi:10.1523/jneurosci.3639-16.2017 · Cited by 139 · see citing works
Self-citation
Diurnal Regulation of Lipid Metabolism and Applications of Circadian Lipidomics
Gooley, J. J., & Chua, E. C.-P. (2014) · Journal of genetics and genomics/Journal of Genetics and Genomics · doi:10.1016/j.jgg.2014.04.001 · Cited by 136 · see citing works
Circadian Influence on Metabolism and Inflammation in Atherosclerosis
McAlpine, C. S., & Świrski, F. K. (2016) · Circulation Research · doi:10.1161/circresaha.116.308034 · Cited by 138 · see citing works
Circadian Rhythms of Melatonin, Cortisol, and Clock Gene Expression During Simulated Night Shift Work
James, F. O., Cermakian, N., & Boivin, D. B. (2007) · Sleep · doi:10.1093/sleep/30.11.1427 · Cited by 135 · see citing works
Circadian disruption in the pathogenesis of metabolic syndrome
Maury, E., Hong, H. K., & Bass, J. (2014) · Diabetes & Metabolism · doi:10.1016/j.diabet.2013.12.005 · Cited by 132 · see citing works
Circadian clocks during embryonic and fetal development
Serón‐Ferré, M., Valenzuela, G. J., & Torres‐Farfan, C. (2007) · Birth Defects Research Part C Embryo Today Reviews · doi:10.1002/bdrc.20101 · Cited by 130 · see citing works
Bone Resorption Is Regulated by Circadian Clock in Osteoblasts
Takarada, T., Xu, C., Ochi, H., Nakazato, R., Yamada, D., Nakamura, S., Kodama, A., Shimba, S., Mieda, M., Fukasawa, K., et al. (2016) · Journal of Bone and Mineral Research · doi:10.1002/jbmr.3053 · Cited by 126 · see citing works
Self-citation
Direct Regulation of Myocardial Triglyceride Metabolism by the Cardiomyocyte Circadian Clock
Tsai, J.-Y., Kienesberger, P. C., Pulinilkunnil, T., Sailors, M. H., Durgan, D. J., Villegas‐Montoya, C., Jahoor, A., Gonzalez, R., Garvey, M. E., Boland, B. B., et al. (2009) · Journal of Biological Chemistry · doi:10.1074/jbc.m109.077800 · Cited by 125 · see citing works
Melatonin and the circadian entrainment of metabolic and hormonal activities in primary isolated adipocytes
Alonso‐Vale, M. I. C., Andreotti, S., Mukai, P. Y., Borges‐Silva, C. N., Peres, S. B., Cipolla‐Neto, J., & Lima, F. B. (2008) · Journal of Pineal Research · doi:10.1111/j.1600-079x.2008.00610.x · Cited by 125 · see citing works
Chronobesity: role of the circadian system in the obesity epidemic
Laermans, J., & Depoortere, I. (2015) · Obesity Reviews · doi:10.1111/obr.12351 · Cited by 126 · see citing works
Loss of Bmal1 leads to uncoupling and impaired glucose-stimulated insulin secretion in β-cells
Lee, J., Kim, M.-S., Li, R., Liu, V. Y., Fu, L., Moore, D. D., Ma, K., & Yechoor, V. K. (2011) · Islets · doi:10.4161/isl.3.6.18157 · Cited by 124 · see citing works
Influence of intrinsic signals and environmental cues on the endocrine control of feeding in fish: Potential application in aquaculture
Volkoff, H., Hoskins, L. J., & Tuziak, S. M. (2009) · General and Comparative Endocrinology · doi:10.1016/j.ygcen.2009.09.001 · Cited by 122 · see citing works
Chronobiological aspects of food intake and metabolism and their relevance on energy balance and weight regulation
Ekmekçioğlu, C., & Touitou, Y. (2010) · Obesity Reviews · doi:10.1111/j.1467-789x.2010.00716.x · Cited by 122 · see citing works
Effects of the biological clock gene Bmal1 on tumour growth and anti-cancer drug activity
Zeng, Z.-L., Wu, M.-W., Sun, J., Sun, Y., Cai, Y., Huang, Y., & Xian, L. (2010) · The Journal of Biochemistry · doi:10.1093/jb/mvq069 · Cited by 121 · see citing works

The clock gene, brain and muscle Arnt‐like 1, regulates adipogenesis via Wnt signaling pathway
Guo, B., Chatterjee, S., Li, L., Kim, J. M., Lee, J., Yechoor, V. K., Minze, L. J., Hsueh, W. A., & Ma, K. (2012) · The FASEB Journal · doi:10.1096/fj.12-205781 · Cited by 168 · see citing works
Deletion of Arntl, a component of the molecular clock, in adipocytes leads to cellular hypertrophy by increasing insulin sensitivity via FGF21
Ishii, H., Kitaura, S., Wada, T., & Shimba, S. (2025) · npj Biological Timing and Sleep · doi:10.1038/s44323-025-00023-7 · Cited by 2 · see citing works
Self-citation
Deficient of a Clock Gene, Brain and Muscle Arnt-Like Protein-1 (BMAL1), Induces Dyslipidemia and Ectopic Fat Formation
Shimba, S., Ogawa, T., Hitosugi, S., Ichihashi, Y., Nakadaira, Y., Kobayashi, M., Tezuka, M., Kosuge, Y., Ishige, K., Ito, Y., et al. (2011) · PLoS ONE · doi:10.1371/journal.pone.0025231 · Cited by 347 · see citing works
Self-citation
The Circadian Clock Regulates Adipogenesis by a Per3 Crosstalk Pathway to Klf15
Aggarwal, A., Costa, M. J. D. C., Rivero‐Gutiérrez, B., Ji, L., Morgan, S. L., & Feldman, B. J. (2017) · Cell Reports · doi:10.1016/j.celrep.2017.11.004 · Cited by 89 · see citing works
308-OR: The Circadian Clock Exerts Coordinated Control of Beige Adipocyte Development via Cytoskeleton-MRTF/SRF Signaling Cascade
Xiong, X., Li, W., Liu, R., Yechoor, V. K., & MA, K. (2022) · Diabetes · doi:10.2337/db22-308-or

Authors: Shimba, S., Ishii, N., Ohta, Y., Ohno, T., Watabe, Y., Hayashi, M., Wada, T., Aoyagi, T., & Tezuka, M.
Affiliations: Nihon University (JP)
ORCID: Shimba, Shigeki
Ohta, Yuki
Venue: Proceedings of the National Academy of Sciences
Publisher: National Academy of Sciences
Published: 2005-08-10
Volume: 102
Issue: 34
Pages: 12071-12076
ISSN: 0027-8424 (ISSN-L)
1091-6490
DOI: 10.1073/pnas.0502383102
PMC: PMC1189312
OpenAlex: W2047902467
Cited by: 536
References: 39
Citations by year: 2026: 15 · 2025: 11 · 2024: 21 · 2023: 21 · 2022: 22 · 2021: 28 · 2020: 19 · 2019: 16 · 2018: 22 · 2017: 24 · 2016: 19 · 2015: 24 · 2014: 33 · 2013: 40 · 2012: 32
FWCI: 9.44
Type: journal article
Language: en
Open access: green
europepmc.org
Locations: Proceedings of the National Academy of Sciences (publishedVersion)
Europe PMC (PubMed Central) (publishedVersion)
PubMed Central (publishedVersion)
PubMed (publishedVersion)
Funders: Nihon University
Ono Medical Research Foundation
Topics: Life Sciences › Neuroscience › Endocrine and Autonomic Systems › Circadian rhythm and melatonin
Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Peroxisome Proliferator-Activated Receptors
Keywords: Bmal1, circadian clock, adipogenesis, lipogenesis, lipid metabolism, 3T3-L1 cells, adipose tissue, circadian rhythm, transcription factors, adipocytes, lipid droplets, gene knockdown, adenoviral gene transfer
MeSH terms: Adenoviridae, Adipose Tissue, Animals, Brain, Circadian Rhythm, Gene Expression Regulation, Genetic Vectors, Male, Mice, Inbred C57BL, Immunoblotting, 3T3 Cells, DNA Primers, Gene Transfer Techniques, Muscle, Skeletal, Reverse Transcriptase Polymerase Chain Reaction, RNA Interference, Chromatin Immunoprecipitation, Adipogenesis, Lipid Metabolism, Mice, Basic Helix-Loop-Helix Proteins, Basic Helix-Loop-Helix Transcription Factors, ARNTL Transcription Factors

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Brain and muscle Arnt-like protein-1 (BMAL1), a component of the molecular clock, regulates adipogenesis

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