Cited by 1,876Neuroscience and Neuropharmacology ResearchAdvanced Fluorescence Microscopy TechniquesNeurogenesis and neuroplasticity mechanisms

The DOI matches its Crossref record; 42 of the 42 references it cites were found in a registry or an index.

Transgenic strategies for combinatorial expression of fluorescent proteins in the nervous system

Livet, Jean, Weissman, Tamily A., Kang, Hyuno, Draft, Ryan W., Lu, Ju, Bennis, Robyn A., Sanes, Joshua R., Lichtman, Jeff W.·2007·Nature

DOI 10.1038/nature06293

Open this paper in CoChat — free account

Abstract

Detailed analysis of neuronal network architecture requires the development of new methods. Here we present strategies to visualize synaptic circuits by genetically labelling neurons with multiple, distinct colours. In Brainbow transgenes, Cre/lox recombination is used to create a stochastic choice of expression between three or more fluorescent proteins (XFPs). Integration of tandem Brainbow copies in transgenic mice yielded combinatorial XFP expression, and thus many colours, thereby providing a way to distinguish adjacent neurons and visualize other cellular interactions. As a demonstration, we reconstructed hundreds of neighbouring axons and multiple synaptic contacts in one small volume of a cerebellar lobe exhibiting approximately 90 colours. The expression in some lines also allowed us to map glial territories and follow glial cells and neurons over time in vivo. The ability of the Brainbow system to label uniquely many individual cells within a population may facilitate the analysis of neuronal circuitry on a large scale.

Integrity signals

Registry

Crossref · 10.1038/nature06293

Cites

42 of 42 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Genome-wide atlas of gene expression in the adult mouse brain
Lein, E. S., Hawrylycz, M. J., Ao, N., Ayres, M., Bensinger, A., Bernard, A., Boe, A. F., Boguski, M. S., Brockway, K. S., Byrnes, E. J., et al. (2007) · Nature · doi:10.1038/nature05453 · Cited by 5,572
Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein
Shaner, N. C., Campbell, R. E., Steinbach, P. A., Giepmans, B. N. G., Palmer, A. E., & Tsien, R. Y. (2004) · Nature Biotechnology · doi:10.1038/nbt1037 · Cited by 4,619
The Cerebellum as a Neuronal Machine
Eccles, J. C., Ito, M., & Szentágothai, J. (1967) · Springer eBooks · doi:10.1007/978-3-662-13147-3 · Cited by 3,229
Cre reporter strains produced by targeted insertion of EYFP and ECFP into the ROSA26 locus
Srinivas, S., Watanabe, T., Lin, C.-S., William, C. M., Tanabe, Y., Jessell, T. M., & Costantini, F. (2001) · BMC Developmental Biology · doi:10.1186/1471-213x-1-4 · Cited by 3,121
Imaging Neuronal Subsets in Transgenic Mice Expressing Multiple Spectral Variants of GFP
Feng, G., Mellor, R. H., Bernstein, M., Keller-Peck, C., Nguyen, Q. T., Wallace, M., Nerbonne, J. M., Lichtman, J. W., & Sanes, J. R. (2000) · Neuron · doi:10.1016/s0896-6273(00)00084-2 · Cited by 3,042
A guide to choosing fluorescent proteins
Shaner, N. C., Steinbach, P. A., & Tsien, R. Y. (2005) · Nature Methods · doi:10.1038/nmeth819 · Cited by 2,823
Cerebellar Cortex
Palay, S. L., & Chan‐Palay, V. (1974) · Springer eBooks · doi:10.1007/978-3-642-65581-4 · Cited by 2,468
A monomeric red fluorescent protein
Campbell, R. E., Tour, O., Palmer, A. E., Steinbach, P. A., Baird, G. S., Zacharias, D. A., & Tsien, R. Y. (2002) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.082243699 · Cited by 2,346
Partitioning of Lipid-Modified Monomeric GFPs into Membrane Microdomains of Live Cells
Zacharias, D. A., Violin, J. D., Newton, A. C., & Tsien, R. Y. (2002) · Science · doi:10.1126/science.1068539 · Cited by 2,270
A gene expression atlas of the central nervous system based on bacterial artificial chromosomes
Gong, S., Zheng, C., Doughty, M. L., Losos, K., Didkovsky, N., Schambra, U. B., Nowak, N. J., Joyner, A., Leblanc, G., Hatten, M. E., et al. (2003) · Nature · doi:10.1038/nature02033 · Cited by 2,108

Show all 42 references

Sorted by citations

Items 401–500 of 1,000

Stimulating Cardiogenesis as a Treatment for Heart Failure
Heallen, T. R., Kadow, Z. A., Jong Heon, K. I. M., Wang, J., & Martin, J. F. (2019) · Circulation Research · doi:10.1161/circresaha.118.313573 · Cited by 68
Evaluating alternative stem cell hypotheses for adult corneal epithelial maintenance
West, J. D. (2015) · World Journal of Stem Cells · doi:10.4252/wjsc.v7.i2.281 · Cited by 67
An optogenetic toolbox for unbiased discovery of functionally connected cells in neural circuits
Förster, D., Maschio, M. D., Laurell, E., & Baier, H. (2017) · Nature Communications · doi:10.1038/s41467-017-00160-z · Cited by 64
Neuroculture
Frazzetto, G., & Anker, S. (2009) · Nature reviews. Neuroscience · doi:10.1038/nrn2736 · Cited by 69
Genetic tools for multicolor imaging in zebrafish larvae
Weber, T., & Köster, R. W. (2013) · Methods · doi:10.1016/j.ymeth.2013.07.028 · Cited by 67
In Vivo Three-Photon Microscopy of Subcortical Structures within an Intact Mouse Brain
Horton, N. G., Wang, K., Kobat, D., Wise, F. W., & Xu, C. (2012) · Conference on Lasers and Electro-Optics · doi:10.1364/cleo_si.2012.cth5c.4 · Cited by 67
Getting a grip on Thy-1 signaling
Barker, T. H., & Hagood, J. S. (2008) · Biochimica et Biophysica Acta (BBA) - Molecular Cell Research · doi:10.1016/j.bbamcr.2008.10.004 · Cited by 67
Cre-Lox Neurogenetics: 20 Years of Versatile Applications in Brain Research and Counting…
Tsien, J. Z. (2016) · Frontiers in Genetics · doi:10.3389/fgene.2016.00019 · Cited by 72
Fate Mapping and Quantitation of Hematopoiesis In Vivo
Höfer, T., Busch, K., Klapproth, K., & Rodewald, H.-R. (2016) · Annual Review of Immunology · doi:10.1146/annurev-immunol-032414-112019 · Cited by 71
Large-scale recording of astrocyte activity
Nimmerjahn, A., & Bergles, D. E. (2015) · Current Opinion in Neurobiology · doi:10.1016/j.conb.2015.01.015 · Cited by 69
Microdissection of neural networks by conditional reporter expression from a Brainbow herpesvirus
Card, J. P., Kobiler, O., McCambridge, J., Ebdlahad, S., Shan, Z. J., Raizada, M. K., Sved, A. F., & Enquist, L. W. (2011) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1015033108 · Cited by 67
Signalling mechanisms regulating axonal branching in vivo
Schmidt, H., & Rathjen, F. G. (2010) · BioEssays · doi:10.1002/bies.201000054 · Cited by 69
Live imaging of multicolor-labeled cells in Drosophila
Boulina, M. E., Samarajeewa, H., Baker, J. D., Kim, M. D., & Chiba, A. (2013) · Development · doi:10.1242/dev.088930 · Cited by 66
Morphometric analysis of astrocytes in brainstem respiratory regions
Sheikhbahaei, S., Morris, B., Collina, J., Anjum, S., Znati, S. A., Gamarra, J., Zhang, R., Gourine, A., & Smith, J. (2018) · The Journal of comparative neurology · doi:10.1002/cne.24472 · Cited by 62
Genetic lineage tracing with multiple DNA recombinases: A user's guide for conducting more precise cell fate mapping studies
Liu, K., Jin, H., & Zhou, B. (2020) · Journal of Biological Chemistry · doi:10.1074/jbc.rev120.011631 · Cited by 69
Lineage tracing using a Cas9-deaminase barcoding system targeting endogenous L1 elements
Hwang, B., Lee, W., Yum, S.-Y., Jeon, Y., Cho, N., Jang, G., & Bang, D. (2019) · Nature Communications · doi:10.1038/s41467-019-09203-z · Cited by 65
Different developmental histories of beta-cells generate functional and proliferative heterogeneity during islet growth
Singh, S. P., Janjuha, S., Hartmann, T., Kayisoglu, Ö., Konantz, J., Birke, S., Murawala, P., Alfar, E. A., Murata, K., Eugster, A., et al. (2017) · Nature Communications · doi:10.1038/s41467-017-00461-3 · Cited by 66
The red flour beetle T. castaneum: elaborate genetic toolkit and unbiased large scale RNAi screening to study insect biology and evolution
Klingler, M., & Bucher, G. (2022) · EvoDevo · doi:10.1186/s13227-022-00201-9 · Cited by 65
Spectrum of Fates: a new approach to the study of the developing zebrafish retina
Almeida, A. D., Boije, H., Chow, R., He, J., Tham, J., Suzuki, S. C., & Harris, W. A. (2014) · Development · doi:10.1242/dev.104760 · Cited by 65
Single-Cell Lineage Tracing Reveals that Oriented Cell Division Contributes to Trabecular Morphogenesis and Regional Specification
Li, J., Miao, L., Shieh, D., Spiotto, E., Li, J., Zhou, B., Paul, A., Schwartz, R. J., Firulli, A. B., Singer, H. A., et al. (2016) · Cell Reports · doi:10.1016/j.celrep.2016.03.012 · Cited by 65
Single-particle combinatorial multiplexed liposome fusion mediated by DNA
Malle, M. G., Löffler, P. M. G., Bohr, S. S.-R., Sletfjerding, M. B., Risgaard, N. A., Jensen, S. B., Zhang, M., Hedegård, P., Vogel, S., & Hatzakis, N. S. (2022) · Nature Chemistry · doi:10.1038/s41557-022-00912-5 · Cited by 66
Symbolic recording of signalling and cis-regulatory element activity to DNA
Chen, W., Choi, J., Li, X., Nathans, J. F., Martin, B., Yang, W., Hamazaki, N., Qiu, C., Lalanne, J., Regalado, S. G., et al. (2024) · Nature · doi:10.1038/s41586-024-07706-4 · Cited by 65
Sparse and combinatorial neuron labelling
Jefferis, G. S. X. E., & Livet, J. (2011) · Current Opinion in Neurobiology · doi:10.1016/j.conb.2011.09.010 · Cited by 63
Circuit reconstruction tools today
Smith, S. J. (2007) · Current Opinion in Neurobiology · doi:10.1016/j.conb.2007.11.004 · Cited by 63
Exclusive multipotency and preferential asymmetric divisions in post-embryonic neural stem cells of the fish retina
Centanin, L., Ander, J.-J., Höckendorf, B., Lust, K., Kellner, T., Kraemer, I., Urbany, C., Hasel, E., Harris, W. A., Simons, B. D., et al. (2014) · Development · doi:10.1242/dev.109892 · Cited by 63
Lineage Analysis of Epidermal Stem CellsPreprint
Alcolea, M. P., & Jones, P. H. (2014) · Cold Spring Harbor Perspectives in Medicine · doi:10.1101/cshperspect.a015206 · Cited by 65
Fibroblastic reticular cell lineage convergence in Peyer’s patches governs intestinal immunity
Prados, A., Onder, L., Cheng, H., Mörbe, U., Lütge, M., Gil‐Cruz, C., Perez‐Shibayama, C., Koliaraki, V., Ludewig, B., & Kollias, G. A. (2021) · Nature Immunology · doi:10.1038/s41590-021-00894-5 · Cited by 66
Benchmarked approaches for reconstruction of in vitro cell lineages and in silico models of C. elegans and M. musculus developmental trees
Gong, W., Granados, A. A., Hu, J., Jones, M. G., Raz, O., Salvador-Martínez, I., Zhang, H., Chow, K.-H. K., Kwak, I.-Y., Retkute, R., et al. (2021) · Cell Systems · doi:10.1016/j.cels.2021.05.008 · Cited by 62
The Laminar Organization of Piriform Cortex Follows a Selective Developmental and Migratory Program Established by Cell Lineage
Martín-López, E., Ishiguro, K., & Greer, C. A. (2017) · Cerebral Cortex · doi:10.1093/cercor/bhx291 · Cited by 62
Tissue-Resident PDGFRα+ Progenitor Cells Contribute to Fibrosis versus Healing in a Context- and Spatiotemporally Dependent Manner
Santini, M. P., Malide, D. A., Hoffman, G. E., Pandey, G. S., d’Escamard, V., Nomura-Kitabayashi, A., Rovira, I. I., Kataoka, H., Ochando, J., Harvey, R. P., et al. (2020) · Cell Reports · doi:10.1016/j.celrep.2019.12.045 · Cited by 58
Azo-Enhanced Raman Scattering for Enhancing the Sensitivity and Tuning the Frequency of Molecular Vibrations
Tang, Y., Zhuang, Y., Zhang, S., Smith, Z. J., Li, Y., Mu, X., Li, M., He, C., Zheng, X., Pan, F., et al. (2021) · ACS Central Science · doi:10.1021/acscentsci.1c00117 · Cited by 64
Differentiation and activation of fibroblastic reticular cells
Lütge, M., Pikor, N. B., & Ludewig, B. (2021) · Immunological Reviews · doi:10.1111/imr.12981 · Cited by 62
Cell cycle dynamics control fluidity of the developing mouse neuroepithelium
Bocanegra-Moreno, L., Singh, A., Hannezo, É., Zagórski, M., & Kicheva, A. (2023) · Nature Physics · doi:10.1038/s41567-023-01977-w · Cited by 62
Expression of transgenes in midbrain dopamine neurons using the tyrosine hydroxylase promoter
Oh, M. S., Hong, S. J., Huh, Y., & Kim, K.-S. (2008) · Gene Therapy · doi:10.1038/gt.2008.148 · Cited by 62
A role for Melanin-Concentrating Hormone in learning and memory
Adamantidis, A., & de Lecea, L. (2009) · Peptides · doi:10.1016/j.peptides.2009.06.024 · Cited by 64
Unveiling the Inner Workings of Live Bacteria Using Super-Resolution Microscopy
Tuson, H. H., & Biteen, J. S. (2014) · Analytical Chemistry · doi:10.1021/ac5041346 · Cited by 63
Correlative Light Electron Microscopy: Connecting Synaptic Structure and Function
Begemann, I., & Galic, M. (2016) · Frontiers in Synaptic Neuroscience · doi:10.3389/fnsyn.2016.00028 · Cited by 63
Intravital microscopy as a tool to study drug delivery in preclinical studies
Amornphimoltham, P., Masedunskas, A., & Weigert, R. (2010) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2010.09.009 · Cited by 61
Multiplexing clonality: combining RGB marking and genetic barcoding
Cornils, K., Thielecke, L., Hüser, S., Forgber, M., Thomaschewski, M., Kleist, N., Hussein, K., Riecken, K., Volz, T., Gerdes, S., et al. (2014) · Nucleic Acids Research · doi:10.1093/nar/gku081 · Cited by 61
The mammary cellular hierarchy and breast cancer
Oakes, S. R., Gallego‐Ortega, D., & Ormandy, C. J. (2014) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-014-1674-4 · Cited by 66
PiggyBac transgenic strategies in the developing chicken spinal cord
Lu, Y., Lin, C., & Wang, X. (2009) · Nucleic Acids Research · doi:10.1093/nar/gkp686 · Cited by 61
Synaptic Laminae in the Visual System: Molecular Mechanisms Forming Layers of Perception
Baier, H. (2013) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev-cellbio-101011-155748 · Cited by 64
Salivary Glands
Aure, M. H., Arany, S., & Ovitt, C. E. (2015) · Journal of Dental Research · doi:10.1177/0022034515599770 · Cited by 62
Switch and Trace: Recombinase Genetics in Zebrafish
Carney, T. J., & Mosimann, C. (2018) · Trends in Genetics · doi:10.1016/j.tig.2018.01.004 · Cited by 55
Semi-Automated Reconstruction of Neural Processes from Large Numbers of Fluorescence Images
Lu, J., Fiala, J. C., & Lichtman, J. W. (2009) · PLoS ONE · doi:10.1371/journal.pone.0005655 · Cited by 61
The Gal4/UAS toolbox in zebrafish: new approaches for defining behavioral circuits
Scott, E. K. (2009) · Journal of Neurochemistry · doi:10.1111/j.1471-4159.2009.06161.x · Cited by 64
Reconciling diverse mammalian pigmentation patterns with a fundamental mathematical model
Mort, R. L., Ross, R. J. H., Hainey, K. J., Harrison, O. J., Keighren, M., Landini, G., Baker, R. E., Painter, K. J., Jackson, I. J., & Yates, C. A. (2016) · Nature Communications · doi:10.1038/ncomms10288 · Cited by 60
Neural crest lineage analysis: from past to future trajectory
Tang, W., & Bronner-Fraser, M. E. (2020) · Development · doi:10.1242/dev.193193 · Cited by 63
Advances in understanding the mechanism of zebrafish heart regeneration
Kikuchi, K. (2014) · Stem Cell Research · doi:10.1016/j.scr.2014.07.003 · Cited by 61
FLP/FRT and Cre/lox recombination technology in C. elegans
Hubbard, E. J. A. (2014) · Methods · doi:10.1016/j.ymeth.2014.05.007 · Cited by 58
Advances in cellular, subcellular, and nanoscale imaging in vitro and in vivo
Wessels, J. T., Yamauchi, K., Hoffman, R. M., & Wouters, F. S. (2010) · Cytometry Part A · doi:10.1002/cyto.a.20931 · Cited by 59
Imaging cancer dynamics in vivo at the tumor and cellular level with fluorescent proteins
Hoffman, R. M. (2008) · Clinical & Experimental Metastasis · doi:10.1007/s10585-008-9205-z · Cited by 59
Tracking cells in their native habitat: lineage tracing in epithelial neoplasia
Alcolea, M. P., & Jones, P. H. (2013) · Nature reviews. Cancer · doi:10.1038/nrc3460 · Cited by 58
ATF3 expression in cardiomyocytes preserves homeostasis in the heart and controls peripheral glucose tolerance
Kalfon, R., Koren, L., Aviram, S., Schwartz, O., Hai, T., & Aronheim, A. (2016) · Cardiovascular Research · doi:10.1093/cvr/cvw228 · Cited by 59
Lasing in Live Mitotic and Non-Phagocytic Cells by Efficient Delivery of Microresonators
Schubert, M., Volckaert, K., Karl, M., Morton, A., Liehm, P., Miles, G. B., Powis, S. J., & Gather, M. C. (2017) · Scientific Reports · doi:10.1038/srep40877 · Cited by 59
Dissecting local circuits in vivo: Integrated optogenetic and electrophysiology approaches for exploring inhibitory regulation of cortical activity
Cardin, J. A. (2011) · Journal of Physiology-Paris · doi:10.1016/j.jphysparis.2011.09.005 · Cited by 56
Cellular, subcellular and functional in vivo labeling of the spinal cord using vital dyes
Romanelli, E., Sorbara, C. D., Nikić, I., Dagkalis, A., Misgeld, T., & Kerschensteiner, M. (2013) · Nature Protocols · doi:10.1038/nprot.2013.022 · Cited by 58
Identification, visualization and clonal analysis of intestinal stem cells in fish
Aghaallaei, N., Gruhl, F., Lischik, C. Q., Wernet, T., Weinhardt, V., Centanin, L., Loosli, F., Baumbach, T., & Wittbrodt, J. (2016) · Development · doi:10.1242/dev.134098 · Cited by 58
Concepts in Light Microscopy of Viruses
Witte, R., Andriasyan, V., Georgi, F., Yakimovich, A., & Greber, U. F. (2018) · Viruses · doi:10.3390/v10040202 · Cited by 59
Precise segmentation of densely interweaving neuron clusters using G-Cut
Li, R., Zhu, M., Li, J., Bienkowski, M. S., Foster, N. N., Xu, H., Ard, T., Bowman, I., Zhou, C., Veldman, M. B., et al. (2019) · Nature Communications · doi:10.1038/s41467-019-09515-0 · Cited by 57
The use of lineage tracing to study kidney injury and regeneration
Romagnani, P., Rinkevich, Y., & Dekel, B. (2015) · Nature Reviews Nephrology · doi:10.1038/nrneph.2015.67 · Cited by 58
Three-dimensional mapping of microcircuit correlation structure
Cotton, R. J., Froudarakis, E., Storer, P., Saggau, P., & Tolias, A. S. (2013) · Frontiers in Neural Circuits · doi:10.3389/fncir.2013.00151 · Cited by 60
Mapping the life histories of T cells
Schumacher, T., Gerlach, C., & van Heijst, J. V. (2010) · Nature reviews. Immunology · doi:10.1038/nri2822 · Cited by 63
Clonal Proliferation and Stochastic Pruning Orchestrate Lymph Node Vasculature Remodeling
Mondor, I., Jorquera, A., Sene, C., Adriouch, S., Adams, R. H., Zhou, B., Wienert, S., Klauschen, F., & Bajénoff, M. (2016) · Immunity · doi:10.1016/j.immuni.2016.09.017 · Cited by 55
Light microscopy based approach for mapping connectivity with molecular specificity
Shen, F. Y., Harrington, M. M., Walker, L. A., Cheng, H. P. J., Boyden, E. S., & Cai, D. (2020) · Nature Communications · doi:10.1038/s41467-020-18422-8 · Cited by 57
Intravital and Kidney Slice Imaging of Podocyte Membrane Dynamics
Brähler, S., Yu, H., Suleiman, H. Y., Krishnan, G. M., Saunders, B. T., Kopp, J. B., Miner, J. H., Zinselmeyer, B. H., & Shaw, A. S. (2016) · Journal of the American Society of Nephrology · doi:10.1681/asn.2015121303 · Cited by 56
High density carbon fiber arrays for chronic electrophysiology, fast scan cyclic voltammetry, and correlative anatomy
Patel, P. R., Popov, P., Caldwell, C. M., Welle, E. J., Egert, D., Pettibone, J. R., Roossien, D. H., Becker, J. B., Berke, J. D., Chestek, C. A., et al. (2020) · Journal of Neural Engineering · doi:10.1088/1741-2552/abb1f6 · Cited by 57
Upconversion optogenetic micro-nanosystem optically controls the secretion of light-responsive bacteria for systemic immunity regulation
Yang, C., Cui, M., Zhang, Y., Pan, H., Liu, J., Wang, S., Ma, N., Chang, J. K., Sun, T., & Wang, H. (2020) · Communications Biology · doi:10.1038/s42003-020-01287-4 · Cited by 56
Functional immunoimaging: the revolution continues
Bousso, P., & Moreau, H. D. (2012) · Nature reviews. Immunology · doi:10.1038/nri3342 · Cited by 63
mBeRFP, an Improved Large Stokes Shift Red Fluorescent Protein
Yang, J., Wang, L., Yang, F., Luo, H., Xu, L., Lu, J., Zeng, S., & Zhang, Z. (2013) · PLoS ONE · doi:10.1371/journal.pone.0064849 · Cited by 56
A Dual Infection Pseudorabies Virus Conditional Reporter Approach to Identify Projections to Collateralized Neurons in Complex Neural Circuits
Card, J. P., Kobiler, O., Ludmir, E. B., Desai, V., Sved, A. F., & Enquist, L. W. (2011) · PLoS ONE · doi:10.1371/journal.pone.0021141 · Cited by 56
Distinct Cell-Autonomous Functions of RETINOBLASTOMA-RELATED in Arabidopsis Stem Cells Revealed by the Brother of Brainbow Clonal Analysis System
Wachsman, G., Heidstra, R., & Scheres, B. (2011) · The Plant Cell · doi:10.1105/tpc.111.086199 · Cited by 56
Single cell transcriptomics: moving towards multi-omics
Song, Y., Xu, X., Wang, W., Tian, T., Zhu, Z., & Yang, C. J. (2019) · The Analyst · doi:10.1039/c8an01852a · Cited by 56
Trans-synaptic Neural Circuit-Tracing with Neurotropic Viruses
Li, J., Liu, T., Dong, Y., Kondoh, K., & Lu, Z. (2019) · Neuroscience Bulletin · doi:10.1007/s12264-019-00374-9 · Cited by 54
Live-imaging fluorescent proteins in mouse embryos: multi-dimensional, multi-spectral perspectives
Nowotschin, S., Eakin, G. S., & Hadjantonakis, A. (2009) · Trends in biotechnology · doi:10.1016/j.tibtech.2009.02.006 · Cited by 56
Three-dimensional reconstructions of mechanosensory end organs suggest a unifying mechanism underlying dynamic, light touch
Handler, A., Zhang, Q., Pang, S., Nguyen, T., Iskols, M., Nolan-Tamariz, M., Cattel, S., Plumb, R., Sanchez, B., Ashjian, K., et al. (2023) · Neuron · doi:10.1016/j.neuron.2023.08.023 · Cited by 56
Optogenetic manipulation of neural and non‐neural functions
Yawo, H., Asano, T., Sakai, S., & Ishizuka, T. (2013) · Development Growth & Differentiation · doi:10.1111/dgd.12053 · Cited by 59
Methods for studying the zebrafish brain: past, present and future
Wyatt, C., Bartoszek, E. M., & Yaksi, E. (2015) · European Journal of Neuroscience · doi:10.1111/ejn.12932 · Cited by 58
A Single Vector Platform for High-Level Gene Transduction of Central Neurons: Adeno-Associated Virus Vector Equipped with the Tet-Off System
Sohn, J., Takahashi, M., Okamoto, S., Ishida, Y., Furuta, T., & Hioki, H. (2017) · PLoS ONE · doi:10.1371/journal.pone.0169611 · Cited by 55
Development of the renal vasculature
Mohamed, T. H., & Sequeira-Lόpez, M. L. S. (2018) · Seminars in Cell and Developmental Biology · doi:10.1016/j.semcdb.2018.06.001 · Cited by 57
Modernization of Golgi staining techniques for high-resolution, 3-dimensional imaging of individual neurons
Vints, K., Vandael, D., Baatsen, P., Pavie, B., Vernaillen, F., Corthout, N., Rybakin, V., Munck, S., & Gounko, N. V. (2019) · Scientific Reports · doi:10.1038/s41598-018-37377-x · Cited by 54
BMP-dependent, injury-induced stem cell niche as a mechanism of heterotopic ossification
Chen, K., Ding, N., Yang, J., Tan, Z., McGuire, T. L., Lu, H., Zhang, K., Berger, D. M. P., Kessler, J. A., & Kan, L. (2019) · Stem Cell Research & Therapy · doi:10.1186/s13287-018-1107-7 · Cited by 56
Readout of fluorescence functional signals through highly scattering tissue
Moretti, C., & Gigan, S. (2020) · Nature Photonics · doi:10.1038/s41566-020-0612-2 · Cited by 55
Temporally multiplexed imaging of dynamic signaling networks in living cells
Qian, Y., Celiker, O. T., Wang, Z., Guner‐Ataman, B., & Boyden, E. S. (2023) · Cell · doi:10.1016/j.cell.2023.11.010 · Cited by 52
Scalable spatial single-cell transcriptomics and translatomics in 3D thick tissue blocks
Sui, X., Lo, J. A., Luo, S., He, Y., Tang, Z., Lin, Z., Barabási, D. L., Zhou, Y., Wang, W. X., Jia Liu, B., et al. (2025) · Nature Methods · doi:10.1038/s41592-025-02867-0 · Cited by 60
Immune cell dynamics in the CNS: Learning from the zebrafish
Oosterhof, N., Boddeke, E., & van Ham, T. J. (2014) · Glia · doi:10.1002/glia.22780 · Cited by 53
Single cell detection of latent cytomegalovirus reactivation in host tissue
Marquardt, A., Halle, S., Seckert, C. K., Lemmermann, N. A. W., Veres, T. Z., Braun, A. C., Maus, U. A., Förster, R., Reddehase, M. J., Messerle, M., et al. (2011) · Journal of General Virology · doi:10.1099/vir.0.029827-0 · Cited by 54
A fourth generation of neuroanatomical tracing techniques: Exploiting the offspring of genetic engineering
Wouterlood, F. G., Bloem, B., Mansvelder, H. D., Luchicchi, A., & Deisseroth, K. A. (2014) · Journal of Neuroscience Methods · doi:10.1016/j.jneumeth.2014.07.021 · Cited by 56
Seeing the whole picture: A comprehensive imaging approach to functional mapping of circuits in behaving zebrafish
Feierstein, C. E., Portugues, R., & Orger, M. B. (2014) · Neuroscience · doi:10.1016/j.neuroscience.2014.11.046 · Cited by 60
Optogenetic deconstruction of sleep-wake circuitry in the brain
Adamantidis, A. (2010) · Frontiers in Molecular Neuroscience · doi:10.3389/neuro.02.031.2009 · Cited by 55
Reconstructing Curvilinear Networks Using Path Classifiers and Integer Programming
Türetken, E., Benmansour, F., Andres, B., Głowacki, P., Pfister, H., & Fua, P. (2016) · IEEE Transactions on Pattern Analysis and Machine Intelligence · doi:10.1109/tpami.2016.2519025 · Cited by 54
Obligate Progression Precedes Lung Adenocarcinoma Dissemination
Caswell, D. R., Chuang, C.-H., Yang, D., Chiou, S., Cheemalavagu, S., Kim-Kiselak, C., Connolly, A. J., & Winslow, M. M. (2014) · Cancer Discovery · doi:10.1158/2159-8290.cd-13-0862 · Cited by 52
Two-photon probes for in vivo multicolor microscopy of the structure and signals of brain cells
Ricard, C., Arroyo, E. D., He, C. X., Portera‐Cailliau, C., Lepousez, G., Canepari, M., & Fiole, D. (2018) · Brain Structure and Function · doi:10.1007/s00429-018-1678-1 · Cited by 54
Towards quantitative and multiplexed in vivo functional cancer genomics
Winters, I. P., Murray, C. W., & Winslow, M. M. (2018) · Nature Reviews Genetics · doi:10.1038/s41576-018-0053-7 · Cited by 54
The Source and Dynamics of Adult Hematopoiesis: Insights from Lineage Tracing
Pucella, J. N., Upadhaya, S., & Reizis, B. (2020) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev-cellbio-020520-114601 · Cited by 53
Determining Lineage Pathways from Cellular Barcoding Experiments
Perié, L., Hodgkin, P. D., Naik, S. H., Schumacher, T. N., de Boer, R. J., & Duffy, K. R. (2014) · Cell Reports · doi:10.1016/j.celrep.2014.01.016 · Cited by 49
Anatomy, development, and plasticity of the neurosecretory hypothalamus in zebrafish
Nagpal, J. K., Herget, U., Choi, M. K., & Ryu, S. (2018) · Cell and Tissue Research · doi:10.1007/s00441-018-2900-4 · Cited by 55
Mastering the use of cellular barcoding to explore cancer heterogeneity
Serrano, A., Berthelet, J., Naik, S. H., & Mérino, D. (2022) · Nature reviews. Cancer · doi:10.1038/s41568-022-00500-2 · Cited by 53
Astrocyte structural heterogeneity in the mouse hippocampus
Viana, J. F., Machado, J. L., Abreu, D. S., Veiga, A., Barsanti, S., Tavares, G., Martins, M., Sardinha, V. M., Guerra‐Gomes, S., Domingos, C., et al. (2023) · Glia · doi:10.1002/glia.24362 · Cited by 61
DSCAM Promotes Refinement in the Mouse Retina through Cell Death and Restriction of Exploring Dendrites
Li, S., Sukeena, J. M., Simmons, A. B., Hansen, E. J., Nuhn, R. E., Samuels, I. S., & Fuerst, P. G. (2015) · Journal of Neuroscience · doi:10.1523/jneurosci.2202-14.2015 · Cited by 56

Authors: Livet, J., Weissman, T. A., Kang, H., Draft, R. W., Lu, J., Bennis, R. A., Sanes, J. R., & Lichtman, J. W.
Affiliations: Harvard University (US)
Corresponding author: Lichtman, Jeff W.
ORCID: Livet, Jean
Weissman, Tamily A.
Kang, Hyuno
Draft, Ryan W.
Lu, Ju
Sanes, Joshua R.
Lichtman, Jeff W.
Venue: Nature
Publisher: Springer Science and Business Media LLC
Published: 2007-11
Volume: 450
Issue: 7166
Pages: 56-62
ISSN: 0028-0836 (ISSN-L)
1476-4687
DOI: 10.1038/nature06293
OpenAlex: W2110232607
Cited by: 1,876
References: 42
Citations by year: 2026: 52 · 2025: 76 · 2024: 68 · 2023: 90 · 2022: 88 · 2021: 88 · 2020: 115 · 2019: 104 · 2018: 124 · 2017: 101 · 2016: 117 · 2015: 101 · 2014: 124 · 2013: 161 · 2012: 110
FWCI: 30.01
Type: journal article
Language: en
Open access: closed
Licence: www.springer.com/tdm (tdm)
Locations: Nature (publishedVersion)
PubMed (publishedVersion)
RePEc: Research Papers in Economics (submittedVersion)
Topics: Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Advanced Fluorescence Microscopy Techniques
Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
Keywords: Brainbow, transgenic mice, fluorescent proteins, Cre-loxP recombination, stochastic gene expression, neuronal circuit mapping, axon reconstruction, synaptic circuits, cerebellum, hippocampus
MeSH terms: Animals, Attachment Sites, Microbiological, Axons, Cell Communication, Cell Line, Cerebellum, Color, Genetic Engineering, Humans, Luminescent Proteins, Mice, Transgenic, Nervous System, Neural Pathways, Neuroglia, Recombination, Genetic, Stochastic Processes, Synapses, Time Factors, Gene Expression, Transgenes, Integrases, Mice

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

Transgenic strategies for combinatorial expression of fluorescent proteins in the nervous system

Abstract

Overview

Integrity signals

Verification

Claims the paper makes

Claims other papers make about this one

Citations

Cites

Cited by

Related

Metadata

Record

About these checks