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Diverse and heritable lineage imprinting of early haematopoietic progenitors

Naik, Shalin H., Perié, Leïla, Swart, Erwin, Gerlach, Carmen, van Rooij, Nienke, de Boer, Rob J., Schumacher, Ton N.·2013·Nature
DOI 10.1038/nature12013
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Abstract

Haematopoietic stem cells (HSCs) and their subsequent progenitors produce blood cells, but the precise nature and kinetics of this production is a contentious issue. In one model, lymphoid and myeloid production branch after the lymphoid-primed multipotent progenitor (LMPP), with both branches subsequently producing dendritic cells. However, this model is based mainly on in vitro clonal assays and population-based tracking in vivo, which could miss in vivo single-cell complexity. Here we avoid these issues by using a new quantitative version of 'cellular barcoding' to trace the in vivo fate of hundreds of LMPPs and HSCs at the single-cell level. These data demonstrate that LMPPs are highly heterogeneous in the cell types that they produce, separating into combinations of lymphoid-, myeloid- and dendritic-cell-biased producers. Conversely, although we observe a known lineage bias of some HSCs, most cellular output is derived from a small number of HSCs that each generates all cell types. Crucially, in vivo analysis of the output of sibling cells derived from single LMPPs shows that they often share a similar fate, suggesting that the fate of these progenitors was imprinted. Furthermore, as this imprinting is also observed for dendritic-cell-biased LMPPs, dendritic cells may be considered a distinct lineage on the basis of separate ancestry. These data suggest a 'graded commitment' model of haematopoiesis, in which heritable and diverse lineage imprinting occurs earlier than pr…

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Cited by

Metadata

Authors
Naik, S. H., Perié, L., Swart, E., Gerlach, C., van Rooij, N., de Boer, R. J., & Schumacher, T. N.
Venue
Nature
Publisher
Springer Science and Business Media LLC
Published
2013-04
Volume
496
Issue
7444
Pages
229-232
ISSN
  • 0028-0836
  • 1476-4687
DOI
10.1038/nature12013
Cited by
408
References
1
Language
en
Licence
www.springer.com/tdm (tdm)

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