The DOI matches its Crossref record; 58 of the 58 references it cites were found in a registry or an index.
Assembly of functionally integrated human forebrain spheroids
Open accessAbstract
The development of the nervous system involves a coordinated succession of events including the migration of GABAergic (γ-aminobutyric-acid-releasing) neurons from ventral to dorsal forebrain and their integration into cortical circuits. However, these interregional interactions have not yet been modelled with human cells. Here we generate three-dimensional spheroids from human pluripotent stem cells that resemble either the dorsal or ventral forebrain and contain cortical glutamatergic or GABAergic neurons. These subdomain-specific forebrain spheroids can be assembled in vitro to recapitulate the saltatory migration of interneurons observed in the fetal forebrain. Using this system, we find that in Timothy syndrome-a neurodevelopmental disorder that is caused by mutations in the Ca V 1.2 calcium channel-interneurons display abnormal migratory saltations. We also show that after migration, interneurons functionally integrate with glutamatergic neurons to form a microphysiological system. We anticipate that this approach will be useful for studying neural development and disease, and for deriving spheroids that resemble other brain regions to assemble circuits in vitro.
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In the citing paper
“With their 3D structures and cell components resembling the human brain, these organoids can replicate the complex brain microenvironment better than 2D in vitro models (15–17, 19)”
From Understanding monocyte-driven neuroinflammation in Alzheimer’s disease using human cortical organoid microphysiological systems (2025) · cites this paper as [19]
Experimental — accuracy not yet measuredIn the citing paper
“The ‘extrinsic’ organoid model whereby patterning is influenced by externally added molecules rather than relying on intrinsic self-organization shows promise with regard to better reproducibility5,6,25.”
From The use of brain organoids to investigate neural development and disease (2017) · cites this paper as [6]
In the citing paper
“These findings were further supported by another report documenting interneuron migration following fusion of dorsal and ventral brain organoids26, and both studies demonstrated disruption of migration by antagonism of C-X-C chemokine receptor type 4 (CXCR4), which has an important role in interneuron migration6,26.”
From The use of brain organoids to investigate neural development and disease (2017) · cites this paper as [6]
In the citing paper
“In the dorsal-ventral fused model mentioned earlier6, cells that migrated from the subpallial spheroid to the cortical spheroid expressed cortical interneuron markers, and whole-cell voltage-clamp recordings demonstrated that these interneuron-like cells can receive excitatory inputs from cells in the dorsal cortical organoids; conversely, the cells in the dorsal cortical organoids can receive”
From The use of brain organoids to investigate neural development and disease (2017) · cites this paper as [6]
In the citing paper
“The organoid-derived interneurons migrated in a manner similar to that of their in vivo counterparts.”
From The use of brain organoids to investigate neural development and disease (2017) · cites this paper as [6]
In the citing paper
“The cells that had migrated into the cortical spheroids also fired action potentials at twice the rate of cells in unfused subpallial spheres or non-migrated cells in the fused organoid, suggesting maturation of membrane properties.”
From The use of brain organoids to investigate neural development and disease (2017) · cites this paper as [6]
In the citing paper
“For example, the fusion of human cortical spheroids and human subpallial spheroids was used to model the migration of interneurons from the subpallium to the cortex6.”
From The use of brain organoids to investigate neural development and disease (2017) · cites this paper as [6]
In the citing paper
“This revealed a cell-autonomous migration defect in Timothy syndrome-derived interneurons.”
From The use of brain organoids to investigate neural development and disease (2017) · cites this paper as [6]
In the citing paper
“Brain organoid models have been shown to recapitulate cell proliferation effects in microcephaly (Thomas et al., 2017) and have been used to study neural migration defects in Timothy syndrome, a rare ASD form caused by mutations in the Cav1.2 calcium channel gene (Birey et al., 2017).”
From Getting to the cores of autism (2019) · cites this paper as Birey et al., 2017
In the citing paper
“We have previously developed an approach to model complex cellular interactions during human brain development by specifying region-specific spheroids or organoids and then fusing them in vitro to generate three-dimensional (3D) assembloids”
From Generation of Functional Human 3D Cortico-Motor Assembloids (2020) · cites this paper as Birey et al., 2017
In the citing paper
“We previously described the generation of 3D spheroids resembling the cerebral cortex (human cortical spheroids [hCS]) (Birey et al., 2017; Paşca et al., 2015; Sloan et al., 2017, 2018), which contain deep and superficial glutamatergic neurons.”
From Generation of Functional Human 3D Cortico-Motor Assembloids (2020) · cites this paper as Birey et al., 2017
In the citing paper
“We verified expression of cortico-spinal-associated markers in hCS, including FEZF2, BCL11B (CTIP2), and SOX5, using single-cell profiling of hCS (Birey et al., 2017) and found a population of cells co-expressing these genes in the glutamatergic neuron cluster but not in a subpallial-derived GABAergic cluster”
From Generation of Functional Human 3D Cortico-Motor Assembloids (2020) · cites this paper as Birey et al., 2017
In the citing paper
“We previously showed the potential of using region-specific spheroids as a modular approach to study human interneuron migration and identify disease phenotypes (Birey et al., 2017).”
From Generation of Functional Human 3D Cortico-Motor Assembloids (2020) · cites this paper as Birey et al., 2017
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Citations
Cites
(2008) · Journal of Machine Learning Research · Cited by 33,051
Differential expression analysis for sequence count data
Anders, S., & Huber, W. (2010) · Genome Biology · doi:10.1186/gb-2010-11-10-r106 · Cited by 15,581
Shrestha, S., & Offer, S. M. (2016) · Medical Epigenetics · doi:10.1159/000444713 · Cited by 8,951
Biological insights from 108 schizophrenia-associated genetic loci
Schizophrenia Working Group of the Psychiatric Genomics Consortium (2014) · Nature · doi:10.1038/nature13595 · Cited by 7,577
Highly parallel genome-wide expression profiling of individual cells using nanoliter droplets.
Macosko, E. Z., Basu, A., Satija, R., Nemesh, J., Shekhar, K., Goldman, M., Tirosh, I., Bialas, A. R., Kamitaki, N., Martersteck, E. M., et al. (2015) · Cell · doi:10.1016/j.cell.2015.05.002 · Cited by 7,110
Spatial reconstruction of single-cell gene expression data
Satija, R., Farrell, J. A., Gennert, D., Schier, A. F., & Regev, A. (2015) · Nature Biotechnology · doi:10.1038/nbt.3192 · Cited by 7,055
Cerebral organoids model human brain development and microcephaly
Lancaster, M. A., Renner, M., Martin, C.-A., Wenzel, D., Bicknell, L. S., Hurles, M. E., Homfray, T., Penninger, J. M., Jackson, A. P., & Knoblich, J. A. (2013) · Nature · doi:10.1038/nature12517 · Cited by 5,372
Meijer, L., Nelson, D. J., Riazanski, V., Gabdoulkhakova, A. G., Héry-Arnaud, G., Berre, R. L., Loaëc, N., Oumata, N., Galons, H., Nowak, E., et al. (2016) · Journal of Innate Immunity · doi:10.1159/000444256 · Cited by 5,008
(2013) · The Lancet · doi:10.1016/s0140-6736(12)62129-1 · Cited by 3,010
Model of autism: increased ratio of excitation/inhibition in key neural systems
Rubenstein, J. L. R., & Merzenich, M. M. (2003) · Genes, Brain and Behavior · doi:10.1034/j.1601-183x.2003.00037.x · Cited by 2,768
Smart-seq2 for sensitive full-length transcriptome profiling in single cells
Picelli, S., Björklund, Å. K., Faridani, O. R., Sagasser, S., Winberg, G., & Sandberg, R. (2013) · Nature Methods · doi:10.1038/nmeth.2639 · Cited by 2,358
Zhang, Y., Sloan, S. A., Clarke, L. E., Caneda, C., Plaza, C. A., Blumenthal, P. D., Vogel, H., Steinberg, G. K., Edwards, M. S. B., Li, G., et al. (2016) · Neuron · doi:10.1016/j.neuron.2015.11.013 · Cited by 2,295
Brain-Region-Specific Organoids Using Mini-bioreactors for Modeling ZIKV Exposure
Qian, X., Nguyen, H. N., Song, M. M., Hadiono, C., Ogden, S. C., Hammack, C., Yao, B., Hamersky, G. R., Jacob, F., Zhong, C., et al. (2016) · Cell · doi:10.1016/j.cell.2016.04.032 · Cited by 2,127
iDISCO: A Simple, Rapid Method to Immunolabel Large Tissue Samples for Volume Imaging
Renier, N., Wu, Z., Simon, D. J., Yang, J. H., Ariel, P., & Tessier‐Lavigne, M. (2014) · Cell · doi:10.1016/j.cell.2014.10.010 · Cited by 1,856
Functional cortical neurons and astrocytes from human pluripotent stem cells in 3D culture
Paşca, A. M., Sloan, S. A., Clarke, L. E., Tian, Y., Makinson, C. D., Huber, N., Kim, C. H., Park, J.-Y., O'Rourke, N. A., Nguyen, K. D., et al. (2015) · Nature Methods · doi:10.1038/nmeth.3415 · Cited by 1,609
Self-citationCaV1.2 Calcium Channel Dysfunction Causes a Multisystem Disorder Including Arrhythmia and Autism
Splawski, I., Timothy, K. W., Sharpe, L. M., Decher, N., Kumar, P., Bloise, R., Napolitano, C., Schwartz, P. J., Joseph, R. M., Condouris, K., et al. (2004) · Cell · doi:10.1016/j.cell.2004.09.011 · Cited by 1,586
Interneuron dysfunction in psychiatric disorders
Marín, O. (2012) · Nature Reviews Neuroscience · doi:10.1038/nrn3155 · Cited by 1,190
Interneuron cell types are fit to function
Kepecs, A., & Fishell, G. (2014) · Nature · doi:10.1038/nature12983 · Cited by 1,110
Cre-dependent selection yields AAV variants for widespread gene transfer to the adult brain
Deverman, B. E., Pravdo, P. L., Simpson, B. P., Kumar, S. R., Chan, K. Y., Banerjee, A., Wu, W.-L., Yang, B., Huber, N., Pasca, S. P., et al. (2016) · Nature Biotechnology · doi:10.1038/nbt.3440 · Cited by 1,061
Self-citationElectroporation and RNA interference in the rodent retina in vivo and in vitro
Matsuda, T., & Cepko, C. L. (2003) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2235688100 · Cited by 1,032
Kadoshima, T., Sakaguchi, H., Nakano, T., Soen, M., Ando, S., Eiraku, M., & Sasai, Y. (2013) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1315710110 · Cited by 999
The origin and specification of cortical interneurons
Wonders, C. P., & Anderson, S. A. (2006) · Nature Reviews Neuroscience · doi:10.1038/nrn1954 · Cited by 980 · cited 7×
“001), saltation length (n= 44 cells from 3 hiPSC lines; one-way ANOVA, interaction F(2, 91)= 3.”
BackgroundMethodInfluentialAccounting for technical noise in single-cell RNA-seq experiments
Brennecke, P., Anders, S., Kim, J. K., Kołodziejczyk, A. A., Zhang, X., Proserpio, V., Baying, B., Benes, V., Teichmann, S. A., Marioni, J. C., et al. (2013) · Nature Methods · doi:10.1038/nmeth.2645 · Cited by 979
The Cellular and Molecular Landscapes of the Developing Human Central Nervous System
Silbereis, J. C., Pochareddy, S., Zhu, Y., Li, M., & Sestan, N. (2016) · Neuron · doi:10.1016/j.neuron.2015.12.008 · Cited by 818
Using induced pluripotent stem cells to investigate cardiac phenotypes in Timothy syndrome
Yazawa, M., Hsueh, B., Jia, X., Pasca, A. M., Bernstein, J. A., Hallmayer, J., & Dolmetsch, R. E. (2011) · Nature · doi:10.1038/nature09855 · Cited by 677
Self-citationDistinct cortical migrations from the medial and lateral ganglionic eminences
Anderson, S. A., Marín, Ó., Horn, C., Jennings, K., & Rubenstein, J. L. R. (2001) · Development · doi:10.1242/dev.128.3.353 · Cited by 651
Severe arrhythmia disorder caused by cardiac L-type calcium channel mutations
Splawski, I., Timothy, K. W., Decher, N., Kumar, P., Sachse, F. B., Beggs, A. H., Sanguinetti, M. C., & Keating, M. T. (2005) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0502506102 · Cited by 624
Maroof, A. M., Keros, S., Tyson, J. A., Ying, S.-W., Ganat, Y. M., Merkle, F. T., Liu, B., Goulburn, A., Stanley, E. G., Elefanty, A. G., et al. (2013) · Cell Stem Cell · doi:10.1016/j.stem.2013.04.008 · Cited by 596
Using iPSC-derived neurons to uncover cellular phenotypes associated with Timothy syndrome
Paşca, S. P., Portmann, T., Voineagu, I., Yazawa, M., Shcheglovitov, A., Paşca, A. M., Cord, B., Palmer, T. D., Chikahisa, S., Nishino, S., et al. (2011) · Nature Medicine · doi:10.1038/nm.2576 · Cited by 585
Self-citationNicholas, C. R., Chen, J., Tang, Y., Southwell, D. G., Chalmers, N., Vogt, D. L., Arnold, C. M., Chen, Y.-J. J., Stanley, E. G., Elefanty, A. G., et al. (2013) · Cell stem cell · doi:10.1016/j.stem.2013.04.005 · Cited by 528
Modeling human cortical development in vitro using induced pluripotent stem cells
Mariani, J., Simonini, M. V., Palejev, D., Tomasini, L., Coppola, G., Szekely, A. M., Horvath, T. L., & Vaccarino, F. M. (2012) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1202944109 · Cited by 526
Pluripotent stem cells in regenerative medicine: challenges and recent progress
Tabar, V. S., & Studer, L. (2014) · Nature Reviews Genetics · doi:10.1038/nrg3563 · Cited by 467
Combinatorial labeling of single cells for gene expression cytometry
Fan, H. C., Fu, G. K., & Fodor, S. P. A. (2015) · Science · doi:10.1126/science.1258367 · Cited by 455
Self-citationCXCR4 Regulates Interneuron Migration in the Developing Neocortex
Stumm, R. K., Zhou, C., Ara, T., Lazarini, F., Dubois-Dalcq, M. E., Nagasawa, T., Höllt, V., & Schulz, S. (2003) · Journal of Neuroscience · doi:10.1523/jneurosci.23-12-05123.2003 · Cited by 450
Bortone, D., & Polleux, F. (2009) · Neuron · doi:10.1016/j.neuron.2009.01.034 · Cited by 387
Bellion, A. (2005) · Journal of Neuroscience · doi:10.1523/jneurosci.1030-05.2005 · Cited by 354
Single-Synapse Analysis of a Diverse Synapse Population: Proteomic Imaging Methods and Markers
Micheva, K. D., Busse, B. L., Weiler, N. C., O’Rourke, N. A., & Smith, S. J. (2010) · Neuron · doi:10.1016/j.neuron.2010.09.024 · Cited by 344
Self-citationKrey, J. F., Pașca, S. P., Shcheglovitov, A., Yazawa, M., Schwemberger, R., Rasmusson, R. L., & Dolmetsch, R. E. (2013) · Nature Neuroscience · doi:10.1038/nn.3307 · Cited by 261
Self-citationVentricle-directed migration in the developing cerebral cortex
Nadarajah, B., Alifragis, P., Wong, R., & Parnavelas, J. G. (2002) · Nature Neuroscience · doi:10.1038/nn813 · Cited by 224
Romero, I. G., Pavlovic, B. J., Hernando-Herraez, I., Zhou, X., Ward, M. C., Banovich, N. E., Kagan, C. L., Burnett, J. E., Huang, C. H., Mitrano, A., et al. (2015) · eLife · doi:10.7554/elife.07103 · Cited by 197
Integration of GABAergic interneurons into cortical cell assemblies: lessons from embryos and adults
Bartolini, G., Ciceri, G., & Marín, O. (2013) · Neuron · doi:10.1016/j.neuron.2013.08.014 · Cited by 185
Sensory experience regulates cortical inhibition by inducing IGF1 in VIP neurons
Mardinly, A. R., Spiegel, I., Patrizi, A., Centofante, E., Bazinet, J. E., Tzeng, C. P., Mandel-Brehm, C., Harmin, D. A., Adesnik, H., Fagiolini, M., et al. (2016) · Nature · doi:10.1038/nature17187 · Cited by 176 · cited 1×
“The values shown are absolute number of cells that migrated to the other side (approximately the same area and thickness was imaged in each session); one-way ANOVA for cells at 17 days after assembly; F(2, 32)= 8.”
Radial glia require PDGFD-PDGFRβ signalling in human but not mouse neocortex
Lui, J. H., Nowakowski, T. J., Pollen, A. A., Javaherian, A., Kriegstein, A. R., & Oldham, M. C. (2014) · Nature · doi:10.1038/nature13973 · Cited by 166
Sun, Y., Paşca, S. P., Portmann, T., Goold, C., Worringer, K. A., Guan, W., Chan, K. C., Gai, H., Vogt, D., Chen, Y.-J. J., et al. (2016) · eLife · doi:10.7554/elife.13073 · Cited by 141
Self-citationGene Expression in Cortical Interneuron Precursors is Prescient of their Mature Function
Batista‐Brito, R., Machold, R. P., Klein, C., & Fishell, G. (2008) · Cerebral Cortex · doi:10.1093/cercor/bhm258 · Cited by 133
Potter, G. B., Petryniak, M. A., Shevchenko, E., McKinsey, G. L., Ekker, M., & Rubenstein, J. L. R. (2008) · Molecular and Cellular Neuroscience · doi:10.1016/j.mcn.2008.10.003 · Cited by 122
GRIP1 and 2 regulate activity-dependent AMPA receptor recycling via exocyst complex interactions
Mao, L., Takamiya, K., Thomas, G., Lin, D.-T., & Huganir, R. L. (2010) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1013494107 · Cited by 94
Tian, Y., Voineagu, I., Paşca, S. P., Won, H., Chandran, V., Horvath, S., Dolmetsch, R. E., & Geschwind, D. H. (2014) · Genome Medicine · doi:10.1186/s13073-014-0075-5 · Cited by 93
Self-citationTCF4, Schizophrenia, and Pitt-Hopkins Syndrome
Blake, D. J., Forrest, M. P., Chapman, R. M., Tinsley, C. L., O’Donovan, M. C., & Owen, M. J. (2010) · Schizophrenia Bulletin · doi:10.1093/schbul/sbq035 · Cited by 74
Generating Human Neurons In Vitro and Using Them to Understand Neuropsychiatric Disease
Pașca, S. P., Panagiotakos, G., & Dolmetsch, R. E. (2014) · Annual Review of Neuroscience · doi:10.1146/annurev-neuro-062012-170328 · Cited by 68
Self-citationYarotskyy, V, & Elmslie, K. S. (2007) · British Journal of Pharmacology · doi:10.1038/sj.bjp.0707414 · Cited by 49
Yarotskyy, V., Gao, G., Du, L., Ganapathi, S. B., Peterson, B. Z., & Elmslie, K. S. (2010) · Journal of Biological Chemistry · doi:10.1074/jbc.m109.076448 · Cited by 36
Zechel, S., Zając, P., Lönnerberg, P., Ibáñez, C. F., & Linnarsson, S. (2014) · Genome biology · doi:10.1186/s13059-014-0486-z · Cited by 34
Circadian abnormalities in mouse models of smith–magenis syndrome: Evidence for involvement of RAI1
Lacaria, M., Gu, W., & Lupski, J. R. (2013) · American Journal of Medical Genetics Part A · doi:10.1002/ajmg.a.35941 · Cited by 28
Uylings, H. B. M., Delalle, I., Petanjek, Z., & Koenderink, M. J. T. (2002) · Neuroembryology and Aging · doi:10.1159/000066268 · Cited by 17
The Development of Phasic and Tonic Inhibition in the Rat Visual Cortex
Jang, H., Cho, K., Park, S., Kim, M.-J., Yoon, S. H., & Rhie, D. (2010) · Korean Journal of Physiology and Pharmacology · doi:10.4196/kjpp.2010.14.6.399 · Cited by 12
Ex Utero Electroporation and Organotypic Slice Culture of Mouse Hippocampal Tissue
Venkataramanappa, S., Simon, R., & Britsch, S. (2015) · Journal of Visualized Experiments · doi:10.3791/52550 · Cited by 4
Generation and Assembly of Forebrain Spheroids
Birey, F., Andersen, J., & Pașca, S. P. (2017) · Protocol Exchange · doi:10.1038/protex.2017.044 · Cited by 1
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Single-Cell Multi-omic Integration Compares and Contrasts Features of Brain Cell Identity
Welch, J. D., Kozareva, V., Ferreira, A., Vanderburg, C., Martin, C., & Macosko, E. Z. (2019) · Cell · doi:10.1016/j.cell.2019.05.006 · Cited by 1,193
Hofer, M., & Lutolf, M. P. (2021) · Nature Reviews Materials · doi:10.1038/s41578-021-00279-y · Cited by 1,184
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Velasco, S., Kedaigle, A. J., Simmons, S. K., Nash, A., Rocha, M., Quadrato, G., Paulsen, B., Nguyen, L., Adiconis, X., Regev, A., et al. (2019) · Nature · doi:10.1038/s41586-019-1289-x · Cited by 1,094
Trujillo, C. A., Gao, R., Negraes, P. D., Gu, J., Buchanan, J., Preissl, S., Wang, A., Wu, W., Haddad, G. G., Chaim, I. A., et al. (2019) · Cell Stem Cell · doi:10.1016/j.stem.2019.08.002 · Cited by 974
Metadata
- Authors
- Birey, F., Andersen, J., Makinson, C. D., Islam, S., Wei, W., Huber, N., Fan, H. C., Metzler, K. R. C., Panagiotakos, G., Thom, N., O’Rourke, N. A., Steinmetz, L. M., Bernstein, J. A., Hallmayer, J., Huguenard, J. R., & Paşca, S. P.
- Affiliations
- Stanford Medicine (US)
- University of California, San Francisco (US)
- ORCID
- Venue
- Nature
- Published
- 2017-04-26
- Volume
- 545
- Issue
- 7652
- Pages
- 54-59
- ISSN
- 0028-0836 (ISSN-L)
- 1476-4687
- PMC
- PMC5805137
- OpenAlex
- W2609473806
- Cited by
- 1,365
- References
- 58
- Citations by year
- 2026: 148 · 2025: 169 · 2024: 164 · 2023: 135 · 2022: 161 · 2021: 165 · 2020: 168 · 2019: 135 · 2018: 84 · 2017: 34
- FWCI
- 48.69
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- Locations
- Nature (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- RePEc: Research Papers in Economics (submittedVersion)
- Funders
- National Institutes of Health
- National Institute of Mental Health (R33MH100717, R01MH107800, R01MH100900)
- National Human Genome Research Institute (P01HG000205)
- National Institute of Neurological Disorders and Stroke (R01NS090911, R01NS034774)
- Topics
- Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Keywords
- human pluripotent stem cells, GABAergic interneurons, interneuron migration, Timothy syndrome, Cav1.2 calcium channel, glutamatergic neurons, brain organoids, neural development
- MeSH terms
- Autistic Disorder, Cell Line, Cell Movement, Cells, Cultured, Female, Humans, Interneurons, Long QT Syndrome, Male, Models, Biological, Neurons, Synapses, Syndactyly, Prosencephalon, Glutamic Acid, Spheroids, Cellular, Pluripotent Stem Cells, Neurogenesis, GABAergic Neurons
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