Cited by 687Monoclonal and Polyclonal Antibodies ResearchT-cell and B-cell ImmunologyGenomics and Phylogenetic Studies

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The promise and challenge of high-throughput sequencing of the antibody repertoire

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Georgiou, George, Ippolito, Gregory C, Beausang, John, Busse, Christian E, Wardemann, Hedda, Quake, Stephen R·2014·Nature Biotechnology

DOI 10.1038/nbt.2782PMC PMC4113560

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Abstract

Efforts to determine the antibody repertoire encoded by B cells in the blood or lymphoid organs using high-throughput DNA sequencing technologies have been advancing at an extremely rapid pace and are transforming our understanding of humoral immune responses. Information gained from high-throughput DNA sequencing of immunoglobulin genes (Ig-seq) can be applied to detect B-cell malignancies with high sensitivity, to discover antibodies specific for antigens of interest, to guide vaccine development and to understand autoimmunity. Rapid progress in the development of experimental protocols and informatics analysis tools is helping to reduce sequencing artifacts, to achieve more precise quantification of clonal diversity and to extract the most pertinent biological information. That said, broader application of Ig-seq, especially in clinical settings, will require the development of a standardized experimental design framework that will enable the sharing and meta-analysis of sequencing data generated by different laboratories.

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Authors: Georgiou, G., Ippolito, G. C., Beausang, J., Busse, C. E., Wardemann, H., & Quake, S. R.
Affiliations: The University of Texas at Austin (US)
Howard Hughes Medical Institute (US)
Max Planck Institute for Infection Biology (DE)
Corresponding author: Georgiou, George
ORCID: Georgiou, George
Ippolito, Gregory C
Busse, Christian E
Wardemann, Hedda
Quake, Stephen R
Venue: Nature Biotechnology
Publisher: Springer Science and Business Media LLC
Published: 2014-01-19
Volume: 32
Issue: 2
Pages: 158-168
ISSN: 0733-222X (ISSN-L)
1087-0156
1546-1696
DOI: 10.1038/nbt.2782
PMC: PMC4113560
OpenAlex: W1993928780
Cited by: 652
References: 141
Citations by year: 2026: 24 · 2025: 34 · 2024: 50 · 2023: 26 · 2022: 39 · 2021: 49 · 2020: 52 · 2019: 56 · 2018: 74 · 2017: 83 · 2016: 78 · 2015: 54 · 2014: 33
FWCI: 12.91
Type: journal article
Language: en
Open access: bronze
doi.org
Licence: www.springernature.com/gp/researchers/text-and-data-mining (tdm)
www.springernature.com/gp/researchers/text-and-data-mining (vor)
Locations: Nature Biotechnology (publishedVersion)
Europe PMC (PubMed Central) (publishedVersion)
PubMed Central (publishedVersion)
PubMed (publishedVersion)
Max Planck Digital Library (submittedVersion)
MPG.PuRe (Max Planck Society) (submittedVersion)
Funders: Howard Hughes Medical Institute
National Institutes of Health
National Institute of Allergy and Infectious Diseases (U54AI057156)
Topics: Life Sciences › Immunology and Microbiology › Immunology › Monoclonal and Polyclonal Antibodies Research
Life Sciences › Immunology and Microbiology › Immunology › T-cell and B-cell Immunology
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Phylogenetic Studies
Keywords: antibody repertoire, B cells, humoral immune response, B-cell malignancies, antibody discovery, vaccine development, autoimmunity, sequencing artifacts
MeSH terms: Animals, Humans, Immunoglobulins, Immunologic Techniques, Models, Immunological, Biomedical Research, Mice, High-Throughput Nucleotide Sequencing

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The promise and challenge of high-throughput sequencing of the antibody repertoire

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