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Cell-type-specific metabolic labeling of nascent proteomes in vivo

Alvarez-Castelao, Beatriz, Schanzenbächer, Christoph T, Hanus, Cyril, Glock, Caspar, tom Dieck, Susanne, Dörrbaum, Aline R, Bartnik, Ina, Nassim-Assir, Belquis, Ciirdaeva, Elena, Mueller, Anke, Dieterich, Daniela C, Tirrell, David A, Langer, Julian D, Schuman, Erin M·2017·Nature Biotechnology

DOI 10.1038/nbt.4016

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Abstract

Although advances in protein labeling methods have made it possible to measure the proteome of mixed cell populations, it has not been possible to isolate cell-type-specific proteomes in vivo. This is because the existing methods for metabolic protein labeling in vivo access all cell types. We report the development of a transgenic mouse line where Cre-recombinase-induced expression of a mutant methionyl-tRNA synthetase (L274G) enables the cell-type-specific labeling of nascent proteins with a non-canonical amino-acid and click chemistry. Using immunoblotting, imaging and mass spectrometry, we use our transgenic mouse to label and analyze proteins in excitatory principal neurons and Purkinje neurons in vitro (brain slices) and in vivo. We discover more than 200 proteins that are differentially regulated in hippocampal excitatory neurons by exposing mice to an environment with enriched sensory cues. Our approach can be used to isolate, analyze and quantitate cell-type-specific proteomes and their dynamics in healthy and diseased tissues.

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Authors: Alvarez-Castelao, B., Schanzenbächer, C. T., Hanus, C., Glock, C., tom Dieck, S., Dörrbaum, A. R., Bartnik, I., Nassim-Assir, B., Ciirdaeva, E., Mueller, A., Dieterich, D. C., Tirrell, D. A., Langer, J. D., & Schuman, E. M.
Affiliations: Max Planck Institute of Biophysics (DE)
Max Planck Institute for Brain Research (DE)
Center for Behavioral Brain Sciences (DE)
California Institute of Technology (US)
Corresponding author: Schuman, Erin M
ORCID: Hanus, Cyril
Glock, Caspar
Dörrbaum, Aline R
Ciirdaeva, Elena
Dieterich, Daniela C
Tirrell, David A
Langer, Julian D
Schuman, Erin M
Venue: Nature Biotechnology
Publisher: Springer Science and Business Media LLC
Published: 2017-11-06
Volume: 35
Issue: 12
Pages: 1196-1201
ISSN: 0733-222X (ISSN-L)
1087-0156
1546-1696
DOI: 10.1038/nbt.4016
OpenAlex: W2765371514
Semantic Scholar: 2688eacdb4dbfc63c3da8b32bdde84e7bfbfaea1
Cited by: 219
References: 50
Citations by year: 2026: 15 · 2025: 30 · 2024: 40 · 2023: 27 · 2022: 25 · 2021: 19 · 2020: 26 · 2019: 17 · 2018: 17 · 2017: 2
FWCI: 8.38
Type: journal article
Language: en
Open access: closed
Licence: www.springer.com/tdm (tdm)
Locations: Nature Biotechnology (publishedVersion)
PubMed (publishedVersion)
CaltechAUTHORS (California Institute of Technology) (submittedVersion)
Max Planck Digital Library (submittedVersion)
MPG.PuRe (Max Planck Society) (submittedVersion)
Funders: Agence Nationale de la Recherche (ANR-16-CE16-0009)
Topics: Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
Physical Sciences › Chemistry › Organic Chemistry › Click Chemistry and Applications
Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Biotin and Related Studies
Keywords: click chemistry, transgenic mice, Cre recombinase, Purkinje cells, hippocampus
MeSH terms: Amino Acids, Animals, Female, Gene Expression Regulation, Male, Methionine-tRNA Ligase, Mice, Transgenic, Neurons, Integrases, Proteome, Proteomics, Mice, Click Chemistry

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