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Draft genome sequence and genetic transformation of the oleaginous alga Nannochloropsis gaditana

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Radakovits, Randor, Jinkerson, Robert E., Fuerstenberg, Susan I., Tae, Hongseok, Settlage, Robert E., Boore, Jeffrey L., Posewitz, Matthew C.·2012·Nature Communications
DOI 10.1038/ncomms1688
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Abstract

The potential use of algae in biofuels applications is receiving significant attention. However, none of the current algal model species are competitive production strains. Here we present a draft genome sequence and a genetic transformation method for the marine microalga Nannochloropsis gaditana CCMP526. We show that N. gaditana has highly favourable lipid yields, and is a promising production organism. The genome assembly includes nuclear (~29 Mb) and organellar genomes, and contains 9,052 gene models. We define the genes required for glycerolipid biogenesis and detail the differential regulation of genes during nitrogen-limited lipid biosynthesis. Phylogenomic analysis identifies genetic attributes of this organism, including unique stramenopile photosynthesis genes and gene expansions that may explain the distinguishing photoautotrophic phenotypes observed. The availability of a genome sequence and transformation methods will facilitate investigations into N. gaditana lipid biosynthesis and permit genetic engineering strategies to further improve this naturally productive alga. Algae show much promise in the production of biofuels owing to their high photoautotrophic biomass and lipid production rates. In this study, the draft genome ofNannochloropsis gaditanaCCMP526 and a method for the transformation of this alga are reported, facilitating the investigation of lipid synthesis and biofuel production.

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Metadata

Authors
Radakovits, R., Jinkerson, R. E., Fuerstenberg, S. I., Tae, H., Settlage, R. E., Boore, J. L., & Posewitz, M. C.
Venue
Nature Communications
Publisher
Springer Science and Business Media LLC
Published
2012-02-21
Volume
3
Issue
1
Pages
686-686
ISSN
2041-1723 (ISSN-L)
DOI
10.1038/ncomms1688
OpenAlex
W2084322857
Cited by
516
References
11
FWCI
19.19
Type
journal article
Language
en
Update notices
correction (2013-09-19)
Open access
hybrid
Licence

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CoChat. (2026). Citation check: Draft genome sequence and genetic transformation of the oleaginous alga Nannochloropsis gaditana. https://app.cochat.ai/papers/10.1038/ncomms1688
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Radakovits, R., Jinkerson, R. E., Fuerstenberg, S. I., Tae, H., Settlage, R. E., Boore, J. L., & Posewitz, M. C. (2012). Draft genome sequence and genetic transformation of the oleaginous alga Nannochloropsis gaditana. Nature Communications. https://doi.org/10.1038/ncomms1688

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