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Molecular memory of prior infections activates the CRISPR/Cas adaptive bacterial immunity system

Datsenko, Kirill A., Pougach, Ksenia, Tikhonov, Anton, Wanner, Barry L., Severinov, Konstantin, Semenova, Ekaterina·2012·Nature Communications
DOI 10.1038/ncomms1937
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  • Mutations on either cas1 or cas2 genes in E. coli CRISPR sequences render spacer acquisition impossible, while overexpression of both Cas1 and Cas2 improved spacer incorporation.

    “Further experiments showed that mutations on either cas1 or cas2 genes in E. coli CRISPR sequences render spacer acquisition impossible, while overexpression of both Cas1 and Cas2 improved spacer incorporation [19]”

    From CRISPR-Cas: Converting A Bacterial Defence Mechanism into A State-of-the-Art Genetic Manipulation Tool (2019) · cites this paper as [19]

    The “Genetic requirements for primed spacer acquisition” section reports that cells lacking cas2 or carrying a Cas1 point mutation did not acquire spacers. It also reports only low-level acquisition when cells expressed just cas1 and cas2, not improved incorporation from overexpressing both. The “An experimental system to monitor spacer acquisition” section describes inducing cas genes more broadly and says there was no gross overproduction of Cas proteins; the copy provides no page numbers for these passages. (LLM, conf=0.87)

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Research works printed but not linked: 28

  1. Mojica, F. J., Díez-Villaseñor, C., Soria, E. & Juez, G. Biological significance of a family of regularly spaced repeats in the genomes of Archaea, Bacteria and mitochondria. Mol. Microbiol. 36, 244–246 (2000). · DOI
  2. Jansen, R., van Embden, J. D., Gaastra, W. & Schouls, L. M. Identification of a novel family of sequence repeats among prokaryotes. OMICS 6, 23–33 (2002). · DOI
  3. Bhaya, D., Davison, M. & Barrangou, R. CRISPR-Cas systems in bacteria and archaea: versatile small RNAs for adaptive defense and regulation. Annu. Rev. Genet. 45, 273–297 (2011). · DOI · Paper page
  4. Jansen, R., Embden, J. D., Gaastra, W. & Schouls, L. M. Identification of genes that are associated with DNA repeats in prokaryotes. Mol. Microbiol. 43, 1565–1575 (2002). · DOI · Paper page
  5. Makarova, K. S. et al. Evolution and classification of the CRISPR-Cas systems. Nat. Rev. Microbiol. 9, 467–477 (2011). · DOI · Paper page
  6. Barrangou, R. et al. CRISPR provides acquired resistance against viruses in prokaryotes. Science 315, 1709–1712 (2007). · DOI · Paper page
  7. van der Oost, J., Jore, M. M., Westra, E. R., Lundgren, M. & Brouns, S. J. CRISPR-based adaptive and heritable immunity in prokaryotes. Trends Biochem. Sci. 34, 401–407 (2009). · DOI
  8. Horvath, P. & Barrangou, R. CRISPR/Cas, the immune system of bacteria and archaea. Science 327, 167–170 (2010). · DOI
  9. Garneau, J. E. et al. The CRISPR/Cas bacterial immune system cleaves bacteriophage and plasmid DNA. Nature 468, 67–71 (2010). · DOI
  10. Deveau, H., Garneau, J. E. & Moineau, S. CRISPR/Cas system and its role in phage-bacteria interactions. Annu. Rev. Microbiol. 64, 475–493 (2010). · DOI
  11. Deveau, H. et al. Phage response to CRISPR-encoded resistance in Streptococcus thermophilus. J. Bacteriol. 190, 1390–1400 (2008). · DOI
  12. Mojica, F. J., Díez-Villaseñor, C., García-Martínez, J. & Almendros, C. Short motif sequences determine the targets of the prokaryotic CRISPR defence system. Microbiology 155, 733–740 (2009). · DOI · Paper page
  13. Semenova, E. et al. Interference by clustered regularly interspaced short palindromic repeat (CRISPR) RNA is governed by a seed sequence. Proc. Natl Acad. Sci. USA 108, 10098–10103 (2011). · DOI · Paper page
  14. Brouns, S. J. et al. Small CRISPR RNAs guide antiviral defense in prokaryotes. Science 321, 960–964 (2008). · DOI · Paper page
  15. Pougach, K. et al. Transcription, processing and function of CRISPR cassettes in Escherichia coli. Mol. Microbiol. 77, 1367–1379 (2010). · DOI
  16. Jore, M. M. et al. Structural basis for CRISPR RNA-guided DNA recognition by Cascade. Nat. Struct. Mol. Biol. 18, 529–536 (2011). · DOI
  17. Wiedenheft, B. et al. Structures of the RNA-guided surveillance complex from a bacterial immune system. Nature 477, 486–489 (2011). · DOI
  18. Westra, E. R. et al. CRISPR immunity relies on the consecutive binding and degradation of negatively supercoiled invader DNA by Cascade and Cas3. Mol. Cell 46, 595–605 (2012). · DOI
  19. Yosef, I., Goren, M. G. & Qimron, U. Proteins and DNA elements essential for the CRISPR adaptation process in Escherichia coli. Nucleic Acids Res doi: 10.1093/nar/gks216 (2012). · DOI · Paper page
  20. Díez-Villaseñor, C., Almendros, C., García-Martínez, J. & Mojica, F. J. Diversity of CRISPR loci in Escherichia coli. Microbiology 156, 1351–1361 (2010). · DOI
  21. Pul, U. et al. Identification and characterization of E. coli CRISPR-cas promoters and their silencing by H-NS. Mol. Microbiol. 75, 1495–1512 (2010). · DOI · Paper page
  22. Westra, E. R. et al. H-NS-mediated repression of CRISPR-based immunity in Escherichia coli K12 can be relieved by the transcription activator LeuO. Mol. Microbiol. 77, 1380–1393 (2010). · DOI
  23. Stern, A., Keren, L., Wurtzel, O., Amitai, G. & Sorek, R. Self-targeting by CRISPR: gene regulation or autoimmunity? Trends Genet. 26, 335–340 (2010). · DOI
  24. Babu, M. et al. A dual function of the CRISPR-Cas system in bacterial antivirus immunity and DNA repair. Mol. Microbiol. 79, 484–502 (2011). · DOI · Paper page
  25. Swarts, D. C., Mosterd, C., van Passel, M. W. & Brouns, S. J. CRISPR interference directs strand specific spacer acquisition. PLoS One 7, e35888 (2012). · DOI
  26. Marraffini, L. A. & Sontheimer, E. J. Self versus non-self discrimination during CRISPR RNA-directed immunity. Nature 463, 568–571 (2010). · DOI
  27. Andersson, A. F. & Banfield, J. F. Virus population dynamics and acquired virus resistance in natural microbial communities. Science 320, 1047–1050 (2008). · DOI
  28. Datsenko, K. A. & Wanner, B. L. One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc. Natl Acad. Sci. USA 97, 6640–6645 (2000). · DOI

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Metadata

Authors
Datsenko, K. A., Pougach, K., Tikhonov, A., Wanner, B. L., Severinov, K., & Semenova, E.
Venue
Nature Communications
Publisher
Springer Science and Business Media LLC
Published
2012-07-10
Volume
3
Issue
1
ISSN
2041-1723
DOI
10.1038/ncomms1937
Cited by
561
References
0
Language
en
Licence

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